Restriction map for
pF1KE4538
(Circular) (Six-base) MAPSORT of: pF1KE4538.seq Check: 4506 from: 1 to: 4973
pF1KE4538 4973 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 1637 2011 1637
Size: 374 4599
Acc65I G'GTAC_C
Cuts at: 1784 1989 1784
Size: 205 4768
AclI AA'CG_TT
Cuts at: 4813 4813
Size: 4973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 310 490 887 1208 1483 1800 1879 2798
Size: 14 180 397 321 275 317 79 919
Cuts at: 2798 3230 3977 4245 296
Size: 432 747 268 1024
Fragments arranged by size:
1024 919 747 432 397 321 317 275
268 180 79 14
AfeI AGC'GCT
Cuts at: 929 1506 929
Size: 577 4396
AflIII A'CryG_T
Cuts at: 357 582 2325 3429 4406 357
Size: 225 1743 1104 977 924
Fragments arranged by size:
1743 1104 977 924 225
AgeI A'CCGG_T
Cuts at: 3361 3361
Size: 4973
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 4973
AlwNI CAG_nnn'CTG
Cuts at: 468 1687 2278 3845 468
Size: 1219 591 1567 1596
Fragments arranged by size:
1596 1567 1219 591
ApaLI G'TGCA_C
Cuts at: 3743 3743
Size: 4973
ApoI r'AATT_y
Cuts at: 506 1387 1977 4326 4582 506
Size: 881 590 2349 256 897
Fragments arranged by size:
2349 897 881 590 256
AseI AT'TA_AT
Cuts at: 20 1823 20
Size: 1803 3170
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
AvaI C'yCGr_G
Cuts at: 1993 1993
Size: 4973
BamHI G'GATC_C
Cuts at: 1860 1998 1860
Size: 138 4835
BanI G'GyrC_C
Cuts at: 1784 1989 2403 2651 2686 1784
Size: 205 414 248 35 4071
Fragments arranged by size:
4071 414 248 205 35
BanII G_rGCy'C
Cuts at: 845 1760 1987 3017 845
Size: 915 227 1030 2801
Fragments arranged by size:
2801 1030 915 227
BbeI G_GCGC'C
Cuts at: 2407 2655 2407
Size: 248 4725
BbsI GAAGACnn'nnnn_
Cuts at: 722 722
Size: 4973
BbvCI CC'TCA_GC
Cuts at: 1076 1076
Size: 4973
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1665 1699 1665
Size: 34 4939
BciVI GTATCCnnnnn_n'
Cuts at: 2871 3632 4973 2871
Size: 761 1341 2871
Fragments arranged by size:
2871 1341 761
BclI T'GATC_A
Cuts at: 2493 2493
Size: 4973
BfrBI ATG'CAT
Cuts at: 1422 1422
Size: 4973
BglII A'GATC_T
Cuts at: 2488 4525 2488
Size: 2037 2936
BlpI GC'TnA_GC
Cuts at: 2085 2085
Size: 4973
Bme1580I G_kGCm'C
Cuts at: 551 2598 2691 3747 551
Size: 2047 93 1056 1777
Fragments arranged by size:
2047 1777 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1029 2185 2266 2601 4745 1029
Size: 1156 81 335 2144 1257
Fragments arranged by size:
2144 1257 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1306 3340 1306
Size: 2034 2939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 1212 1276 1774 1789 1084
Size: 128 64 498 15 4268
Fragments arranged by size:
4268 498 128 64 15
Bpu10I CC'TnA_GC
Cuts at: 1076 1076
Size: 4973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2149 3028 3520 3818 2149
Size: 879 492 298 3304
Fragments arranged by size:
3304 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4677 4677
Size: 4973
BsaAI yAC'GTr
Cuts at: 1564 2326 1564
Size: 762 4211
BsaBI GATnn'nnATC
Cuts at: 6 1232 2487 4524 6
Size: 1226 1255 2037 455
Fragments arranged by size:
2037 1255 1226 455
BsaHI Gr'CG_yC
Cuts at: 2404 2652 3354 4850 2404
Size: 248 702 1496 2527
Fragments arranged by size:
2527 1496 702 248
BsaWI w'CCGG_w
Cuts at: 920 2162 2683 3361 3635 3782 4193 920
Size: 1242 521 678 274 147 411 1700
Fragments arranged by size:
1700 1242 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1619 1649 1619
Size: 30 4943
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 148 637 1265 1499 126
Size: 22 489 628 234 3600
Fragments arranged by size:
3600 628 489 234 22
BseYI C'CCAG_C
Cuts at: 550 2398 3156 3733 4229 550
Size: 1848 758 577 496 1294
Fragments arranged by size:
1848 1294 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 366
Size: 4973
BsiEI CG_ry'CG
Cuts at: 104 2515 2561 3769 104
Size: 2411 46 1208 1308
Fragments arranged by size:
2411 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 845 1760 1987 2765 2955 3747 845
Size: 915 227 778 190 792 2071
Fragments arranged by size:
2071 915 792 778 227 190
BsmI GAATG_Cn'
Cuts at: 1610 1610
Size: 4973
Bsp1286I G_dGCh'C
Cuts at: 551 845 1760 1987 2598 2691 2765 2955 3017
Size: 294 915 227 611 93 74 190 62
Cuts at: 3017 3747 551
Size: 730 1777
Fragments arranged by size:
1777 915 730 611 294 227 190 93
74 62
BspEI T'CCGG_A
Cuts at: 920 4193 920
Size: 3273 1700
BspHI T'CATG_A
Cuts at: 4149 4149
Size: 4973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2023 2237 2539 2920 2023
Size: 214 302 381 4076
Fragments arranged by size:
4076 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3265 3319 4803 4968 50
Size: 3215 54 1484 165 55
Fragments arranged by size:
3215 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2885 2885
Size: 4973
BsrFI r'CCGG_y
Cuts at: 2971 3152 3361 2971
Size: 181 209 4583
Fragments arranged by size:
4583 209 181
BssSI C'ACGA_G
Cuts at: 3244 3602 3244
Size: 358 4615
BstAPI GCAn_nnn'nTGC
Cuts at: 2350 2350
Size: 4973
BstBI TT'CG_AA
Cuts at: 3334 3334
Size: 4973
BstYI r'GATC_y
Cuts at: 1 1227 1860 1998 2465 2488 2823 3069 4081
Size: 1226 633 138 467 23 335 246 1012
Cuts at: 4081 4167 4179 4525 1
Size: 86 12 346 449
Fragments arranged by size:
1226 1012 633 467 449 346 335 246
138 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2923 3104 3267 2923
Size: 181 163 4629
Fragments arranged by size:
4629 181 163
BtsI GCAGTG_nn'
Cuts at: 815 2216 815
Size: 1401 3572
ClaI AT'CG_AT
Cuts at: 1236 1425 1236
Size: 189 4784
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 983 1018 983
Size: 35 4938
DraI TTT'AAA
Cuts at: 1972 1972
Size: 4973
DraIII CAC_nnn'GTG
Cuts at: 344 344
Size: 4973
DrdI GACnn_nn'nnGTC
Cuts at: 2679 3537 2679
Size: 858 4115
EaeI y'GGCC_r
Cuts at: 887 1598 2558 2732 3123 3150 4495 887
Size: 711 960 174 391 27 1345 1365
Fragments arranged by size:
1365 1345 960 711 391 174 27
EagI C'GGCC_G
Cuts at: 2558 2558
Size: 4973
EarI CTCTTCn'nnn_
Cuts at: 272 554 1313 2996 3206 272
Size: 282 759 1683 210 2039
Fragments arranged by size:
2039 1683 759 282 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 3181 3491 3637 4783 145
Size: 3036 310 146 1146 335
Fragments arranged by size:
3036 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 843 1758 1985 843
Size: 915 227 3831
Fragments arranged by size:
3831 915 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 310 490 887 1084 1208 1212 1276 1483
Size: 14 180 397 197 124 4 64 207
Cuts at: 1483 1774 1789 1800 1879 2798 3230 3977 4245
Size: 291 15 11 79 919 432 747 268
Cuts at: 4245 296
Size: 1024
Fragments arranged by size:
1024 919 747 432 397 291 268 207
197 180 124 79 64 15 14 11
4
EcoO109I rG'GnC_Cy
Cuts at: 2112 2112
Size: 4973
EcoRI G'AATT_C
Cuts at: 1977 1977
Size: 4973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1176 2992 1176
Size: 1816 3157
FspI TGC'GCA
Cuts at: 418 2754 418
Size: 2336 2637
HaeII r_GCGC'y
Cuts at: 931 1508 2407 2655 3677 4656 931
Size: 577 899 248 1022 979 1248
Fragments arranged by size:
1248 1022 979 899 577 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 526 558 1619 1651 2815 2845 2847 2877 526
Size: 32 1061 32 1164 30 2 30 2622
Fragments arranged by size:
2622 1164 1061 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2012 2012
Size: 4973
HindIII A'AGCT_T
Cuts at: 690 1055 1220 690
Size: 365 165 4443
Fragments arranged by size:
4443 365 165
KasI G'GCGC_C
Cuts at: 2403 2651 2403
Size: 248 4725
KpnI G_GTAC'C
Cuts at: 1788 1993 1788
Size: 205 4768
MluI A'CGCG_T
Cuts at: 4406 4406
Size: 4973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 932 3644 3828 932
Size: 2712 184 2077
Fragments arranged by size:
2712 2077 184
MscI TGG'CCA
Cuts at: 889 2734 889
Size: 1845 3128
MslI CAynn'nnrTG
Cuts at: 424 895 2962 3089 424
Size: 471 2067 127 2308
Fragments arranged by size:
2308 2067 471 127
MspA1I CmG'CkG
Cuts at: 213 780 2084 2398 2758 3771 4016 213
Size: 567 1304 314 360 1013 245 1170
Fragments arranged by size:
1304 1170 1013 567 360 314 245
NaeI GCC'GGC
Cuts at: 3154 3154
Size: 4973
NarI GG'CG_CC
Cuts at: 2404 2652 2404
Size: 248 4725
NgoMIV G'CCGG_C
Cuts at: 3152 3152
Size: 4973
NsiI A_TGCA'T
Cuts at: 1424 1424
Size: 4973
NspI r_CATG'y
Cuts at: 361 586 2026 2526 3433 361
Size: 225 1440 500 907 1901
Fragments arranged by size:
1901 1440 907 500 225
PciI A'CATG_T
Cuts at: 357 582 3429 357
Size: 225 2847 1901
Fragments arranged by size:
2847 1901 225
PfoI T'CCnGG_A
Cuts at: 257 257
Size: 4973
PmeI GTTT'AAAC
Cuts at: 1972 1972
Size: 4973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 4112
Size: 32 4941
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 458 490 458
Size: 32 4941
PstI C_TGCA'G
Cuts at: 155 2020 2705 155
Size: 1865 685 2423
Fragments arranged by size:
2423 1865 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4973
PvuII CAG'CTG
Cuts at: 780 2398 2758 780
Size: 1618 360 2995
Fragments arranged by size:
2995 1618 360
RsrII CG'GwC_CG
Cuts at: 3168 3168
Size: 4973
SacI G_AGCT'C
Cuts at: 845 1760 1987 845
Size: 915 227 3831
Fragments arranged by size:
3831 915 227
SalI G'TCGA_C
Cuts at: 2010 2010
Size: 4973
SapI GCTCTTCn'nnn_
Cuts at: 554 2996 3206 554
Size: 2442 210 2321
Fragments arranged by size:
2442 2321 210
SbfI CC_TGCA'GG
Cuts at: 2020 2020
Size: 4973
ScaI AGT'ACT
Cuts at: 1018 4490 1018
Size: 3472 1501
SfcI C'TryA_G
Cuts at: 33 151 2016 2701 3694 3885 33
Size: 118 1865 685 993 191 1121
Fragments arranged by size:
1865 1121 993 685 191 118
SfoI GGC'GCC
Cuts at: 2405 2653 2405
Size: 248 4725
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
SmaI CCC'GGG
Cuts at: 1995 1995
Size: 4973
SmlI C'TyrA_G
Cuts at: 2128 3043 3535 3797 2128
Size: 915 492 262 3304
Fragments arranged by size:
3304 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4973
SphI G_CATG'C
Cuts at: 2026 2526 2026
Size: 500 4473
SspI AAT'ATT
Cuts at: 750 750
Size: 4973
StyI C'CwwG_G
Cuts at: 914 2107 2461 914
Size: 1193 354 3426
Fragments arranged by size:
3426 1193 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 436 2556 2689 3148 3357 436
Size: 2120 133 459 209 2052
Fragments arranged by size:
2120 2052 459 209 133
TatI w'GTAC_w
Cuts at: 1016 4488 1016
Size: 3472 1501
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4490 4522 4490
Size: 32 4941
Tth111I GACn'n_nGTC
Cuts at: 2770 2770
Size: 4973
XbaI T'CTAG_A
Cuts at: 2004 2004
Size: 4973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2468
Size: 4973
XmaI C'CCGG_G
Cuts at: 1993 1993
Size: 4973
XmnI GAAnn'nnTTC
Cuts at: 571 1221 571
Size: 650 4323
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI