Restriction map for
pF1KE4513
(Circular) (Six-base) MAPSORT of: pF1KE4513.seq Check: 256 from: 1 to: 4739
pF1KE4513 4739 bp
With 182 enzymes: SgfI *
December 8, 2011 13:20 ..
AccI GT'mk_AC
Cuts at: 407 504 739 1777 407
Size: 97 235 1038 3369
Fragments arranged by size:
3369 1038 235 97
Acc65I G'GTAC_C
Cuts at: 1755 1755
Size: 4739
AclI AA'CG_TT
Cuts at: 4579 4579
Size: 4739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 597 946 1368 2564 2996 3743 4011 490
Size: 107 349 422 1196 432 747 268 1218
Fragments arranged by size:
1218 1196 747 432 422 349 268 107
AflII C'TTAA_G
Cuts at: 1236 1236
Size: 4739
AflIII A'CryG_T
Cuts at: 2091 3195 4172 2091
Size: 1104 977 2658
Fragments arranged by size:
2658 1104 977
AgeI A'CCGG_T
Cuts at: 1117 3127 1117
Size: 2010 2729
AhdI GACnn_n'nnGTC
Cuts at: 1091 1091
Size: 4739
AleI CACnn'nnGTG
Cuts at: 1312 1312
Size: 4739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 4739
AlwNI CAG_nnn'CTG
Cuts at: 162 2044 3611 162
Size: 1882 1567 1290
Fragments arranged by size:
1882 1567 1290
ApaI G_GGCC'C
Cuts at: 304 304
Size: 4739
ApaLI G'TGCA_C
Cuts at: 3509 3509
Size: 4739
ApoI r'AATT_y
Cuts at: 1156 1743 4092 4348 1156
Size: 587 2349 256 1547
Fragments arranged by size:
2349 1547 587 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4739
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
AvaI C'yCGr_G
Cuts at: 748 1558 1759 748
Size: 810 201 3728
Fragments arranged by size:
3728 810 201
BamHI G'GATC_C
Cuts at: 1694 1764 1694
Size: 70 4669
BanI G'GyrC_C
Cuts at: 323 711 1755 2169 2417 2452 323
Size: 388 1044 414 248 35 2610
Fragments arranged by size:
2610 1044 414 388 248 35
BanII G_rGCy'C
Cuts at: 304 521 998 1729 1753 2783 304
Size: 217 477 731 24 1030 2260
Fragments arranged by size:
2260 1030 731 477 217 24
BbeI G_GCGC'C
Cuts at: 2173 2421 2173
Size: 248 4491
BbsI GAAGACnn'nnnn_
Cuts at: 633 843 942 1628 633
Size: 210 99 686 3744
Fragments arranged by size:
3744 686 210 99
BciVI GTATCCnnnnn_n'
Cuts at: 1058 2637 3398 4739 1058
Size: 1579 761 1341 1058
Fragments arranged by size:
1579 1341 1058 761
BclI T'GATC_A
Cuts at: 2259 2259
Size: 4739
BglI GCCn_nnn'nGGC
Cuts at: 398 1300 398
Size: 902 3837
BglII A'GATC_T
Cuts at: 221 1019 2254 4291 221
Size: 798 1235 2037 669
Fragments arranged by size:
2037 1235 798 669
BlpI GC'TnA_GC
Cuts at: 982 1851 982
Size: 869 3870
Bme1580I G_kGCm'C
Cuts at: 304 1319 2364 2457 3513 304
Size: 1015 1045 93 1056 1530
Fragments arranged by size:
1530 1056 1045 1015 93
BmrI ACTGGGnnnn_n'
Cuts at: 187 477 801 1951 2032 2367 4511 187
Size: 290 324 1150 81 335 2144 415
Fragments arranged by size:
2144 1150 415 335 324 290 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 997 1186 1715 3106 997
Size: 189 529 1391 2630
Fragments arranged by size:
2630 1391 529 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1713 1165
Size: 548 4191
Bpu10I CC'TnA_GC
Cuts at: 682 682
Size: 4739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1915 2794 3286 3584 1915
Size: 879 492 298 3070
Fragments arranged by size:
3070 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 524 1582 4443 524
Size: 1058 2861 820
Fragments arranged by size:
2861 1058 820
BsaAI yAC'GTr
Cuts at: 2092 2092
Size: 4739
BsaBI GATnn'nnATC
Cuts at: 6 1371 2253 4290 6
Size: 1365 882 2037 455
Fragments arranged by size:
2037 1365 882 455
BsaHI Gr'CG_yC
Cuts at: 2170 2418 3120 4616 2170
Size: 248 702 1496 2293
Fragments arranged by size:
2293 1496 702 248
BsaWI w'CCGG_w
Cuts at: 601 1061 1117 1928 2449 3127 3401 3548 3959
Size: 460 56 811 521 678 274 147 411
Cuts at: 3959 601
Size: 1381
Fragments arranged by size:
1381 811 678 521 460 411 274 147
56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 167 197 256 286 506 536 167
Size: 30 59 30 220 30 4370
Fragments arranged by size:
4370 220 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1006 1546 1006
Size: 540 4199
BseYI C'CCAG_C
Cuts at: 401 1209 2164 2922 3499 3995 401
Size: 808 955 758 577 496 1145
Fragments arranged by size:
1145 955 808 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 626
Size: 4739
BsiEI CG_ry'CG
Cuts at: 104 2281 2327 3535 104
Size: 2177 46 1208 1308
Fragments arranged by size:
2177 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 293 688 998 1753 2531 2721 3513 293
Size: 395 310 755 778 190 792 1519
Fragments arranged by size:
1519 792 778 755 395 310 190
Bsp1286I G_dGCh'C
Cuts at: 293 304 521 688 998 1319 1729 1753 2364
Size: 11 217 167 310 321 410 24 611
Cuts at: 2364 2457 2531 2721 2783 3513 293
Size: 93 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 410 321 310 217 190
167 93 74 62 24 11
BspEI T'CCGG_A
Cuts at: 1061 3959 1061
Size: 2898 1841
BspHI T'CATG_A
Cuts at: 3915 3915
Size: 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 490 577 587 1016 1789 2003 2305 2686
Size: 269 87 10 429 773 214 302 381
Cuts at: 2686 221
Size: 2274
Fragments arranged by size:
2274 773 429 381 302 269 214 87
10
BsrBI CCG'CTC
Cuts at: 50 3031 3085 4569 4734 50
Size: 2981 54 1484 165 55
Fragments arranged by size:
2981 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2651 2651
Size: 4739
BsrFI r'CCGG_y
Cuts at: 1117 1250 2737 2918 3127 1117
Size: 133 1487 181 209 2729
Fragments arranged by size:
2729 1487 209 181 133
BsrGI T'GTAC_A
Cuts at: 336 336
Size: 4739
BssSI C'ACGA_G
Cuts at: 520 3010 3368 520
Size: 2490 358 1891
Fragments arranged by size:
2490 1891 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2116 2116
Size: 4739
BstBI TT'CG_AA
Cuts at: 3100 3100
Size: 4739
BstXI CCAn_nnnn'nTGG
Cuts at: 636 636
Size: 4739
BstYI r'GATC_y
Cuts at: 1 122 221 1019 1694 1764 2231 2254 2589
Size: 121 99 798 675 70 467 23 335
Cuts at: 2589 2835 3847 3933 3945 4291 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 798 675 467 449 346 335 246
121 99 86 70 23 12
BstZ17I GTA'TAC
Cuts at: 505 505
Size: 4739
BtgI C'CryG_G
Cuts at: 296 380 296
Size: 84 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2689 2870 3033 2689
Size: 181 163 4395
Fragments arranged by size:
4395 181 163
BtsI GCAGTG_nn'
Cuts at: 1572 1982 1572
Size: 410 4329
DraI TTT'AAA
Cuts at: 1738 1738
Size: 4739
DrdI GACnn_nn'nnGTC
Cuts at: 879 2445 3303 879
Size: 1566 858 2315
Fragments arranged by size:
2315 1566 858
EaeI y'GGCC_r
Cuts at: 200 549 768 1028 2324 2498 2889 2916 4261
Size: 349 219 260 1296 174 391 27 1345
Cuts at: 4261 200
Size: 678
Fragments arranged by size:
1345 1296 678 391 349 260 219 174
27
EagI C'GGCC_G
Cuts at: 2324 2324
Size: 4739
EarI CTCTTCn'nnn_
Cuts at: 962 1399 1582 2762 2972 962
Size: 437 183 1180 210 2729
Fragments arranged by size:
2729 1180 437 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 332 2947 3257 3403 4549 332
Size: 2615 310 146 1146 522
Fragments arranged by size:
2615 1146 522 310 146
EcoICRI GAG'CTC
Cuts at: 996 1751 996
Size: 755 3984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 597 946 1165 1368 1713 2564 2996 3743
Size: 107 349 219 203 345 851 432 747
Cuts at: 3743 4011 490
Size: 268 1218
Fragments arranged by size:
1218 851 747 432 349 345 268 219
203 107
EcoNI CCTnn'n_nnAGG
Cuts at: 1012 1689 1012
Size: 677 4062
EcoO109I rG'GnC_Cy
Cuts at: 280 921 1878 280
Size: 641 957 3141
Fragments arranged by size:
3141 957 641
EcoRI G'AATT_C
Cuts at: 1743 1743
Size: 4739
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4739
FspI TGC'GCA
Cuts at: 2520 2520
Size: 4739
HaeII r_GCGC'y
Cuts at: 2173 2421 3443 4422 2173
Size: 248 1022 979 2490
Fragments arranged by size:
2490 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1077 1109 1280 1312 1581 1613 2581 2611 2613
Size: 32 171 32 269 32 968 30 2
Cuts at: 2613 2643 1077
Size: 30 3173
Fragments arranged by size:
3173 968 269 171 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 740 827 1167 1778 740
Size: 87 340 611 3701
Fragments arranged by size:
3701 611 340 87
HpaI GTT'AAC
Cuts at: 827 827
Size: 4739
KasI G'GCGC_C
Cuts at: 2169 2417 2169
Size: 248 4491
KpnI G_GTAC'C
Cuts at: 1759 1759
Size: 4739
MluI A'CGCG_T
Cuts at: 4172 4172
Size: 4739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 3410 3594 485
Size: 2925 184 1630
Fragments arranged by size:
2925 1630 184
MscI TGG'CCA
Cuts at: 202 770 1030 2500 202
Size: 568 260 1470 2441
Fragments arranged by size:
2441 1470 568 260
MslI CAynn'nnrTG
Cuts at: 634 867 1312 2728 2855 634
Size: 233 445 1416 127 2518
Fragments arranged by size:
2518 1416 445 233 127
MspA1I CmG'CkG
Cuts at: 596 1270 1388 1491 1850 2164 2524 3537 3782
Size: 674 118 103 359 314 360 1013 245
Cuts at: 3782 596
Size: 1553
Fragments arranged by size:
1553 1013 674 360 359 314 245 118
103
NaeI GCC'GGC
Cuts at: 1252 2920 1252
Size: 1668 3071
NarI GG'CG_CC
Cuts at: 2170 2418 2170
Size: 248 4491
NcoI C'CATG_G
Cuts at: 296 380 296
Size: 84 4655
NgoMIV G'CCGG_C
Cuts at: 1250 2918 1250
Size: 1668 3071
NspI r_CATG'y
Cuts at: 169 1792 2292 3199 169
Size: 1623 500 907 1709
Fragments arranged by size:
1709 1623 907 500
PciI A'CATG_T
Cuts at: 3195 3195
Size: 4739
PflMI CCAn_nnn'nTGG
Cuts at: 890 890
Size: 4739
PfoI T'CCnGG_A
Cuts at: 125 125
Size: 4739
PmeI GTTT'AAAC
Cuts at: 1738 1738
Size: 4739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4707
PspOMI G'GGCC_C
Cuts at: 300 300
Size: 4739
PstI C_TGCA'G
Cuts at: 221 574 824 1013 1786 2471 221
Size: 353 250 189 773 685 2489
Fragments arranged by size:
2489 773 685 353 250 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4739
PvuII CAG'CTG
Cuts at: 1388 2164 2524 1388
Size: 776 360 3603
Fragments arranged by size:
3603 776 360
RsrII CG'GwC_CG
Cuts at: 2934 2934
Size: 4739
SacI G_AGCT'C
Cuts at: 998 1753 998
Size: 755 3984
SalI G'TCGA_C
Cuts at: 738 1776 738
Size: 1038 3701
SapI GCTCTTCn'nnn_
Cuts at: 2762 2972 2762
Size: 210 4529
SbfI CC_TGCA'GG
Cuts at: 1786 1786
Size: 4739
ScaI AGT'ACT
Cuts at: 4256 4256
Size: 4739
SfcI C'TryA_G
Cuts at: 33 217 570 820 1009 1131 1702 1782 2467
Size: 184 353 250 189 122 571 80 685
Cuts at: 2467 3460 3651 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 571 353 250 191 189
184 122 80
SfoI GGC'GCC
Cuts at: 2171 2419 2171
Size: 248 4491
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
SmaI CCC'GGG
Cuts at: 1761 1761
Size: 4739
SmlI C'TyrA_G
Cuts at: 1236 1894 2809 3301 3563 1236
Size: 658 915 492 262 2412
Fragments arranged by size:
2412 915 658 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4739
SphI G_CATG'C
Cuts at: 1792 2292 1792
Size: 500 4239
StuI AGG'CCT
Cuts at: 1684 1721 1684
Size: 37 4702
StyI C'CwwG_G
Cuts at: 283 296 380 419 1025 1031 1319 1873 2227
Size: 13 84 39 606 6 288 554 354
Cuts at: 2227 283
Size: 2795
Fragments arranged by size:
2795 606 554 354 288 84 39 13
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 385 699 1135 2322 2455 2914 3123 276
Size: 109 314 436 1187 133 459 209 1892
Fragments arranged by size:
1892 1187 459 436 314 209 133 109
TatI w'GTAC_w
Cuts at: 336 1040 4254 336
Size: 704 3214 821
Fragments arranged by size:
3214 821 704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 256 288 436 468 506 538 1466 1498 4256
Size: 32 148 32 38 32 928 32 2758
Cuts at: 4256 4288 256
Size: 32 707
Fragments arranged by size:
2758 928 707 148 38 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2536 2536
Size: 4739
XbaI T'CTAG_A
Cuts at: 1770 1770
Size: 4739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 891 1189 2234 891
Size: 298 1045 3396
Fragments arranged by size:
3396 1045 298
XmaI C'CCGG_G
Cuts at: 1759 1759
Size: 4739
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BssHII
BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI