Restriction map for
pF1KE4446
(Circular) (Six-base) MAPSORT of: pF1KE4446.seq Check: 2674 from: 1 to: 4496
pF1KE4446 4496 bp
With 182 enzymes: SgfI *
October 12, 2018 16:45 ..
AccI GT'mk_AC
Cuts at: 1534 1534
Size: 4496
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2753 3500 3768 2321
Size: 432 747 268 3049
Fragments arranged by size:
3049 747 432 268
AfeI AGC'GCT
Cuts at: 1213 1213
Size: 4496
AflIII A'CryG_T
Cuts at: 1110 1183 1848 2952 3929 1110
Size: 73 665 1104 977 1677
Fragments arranged by size:
1677 1104 977 665 73
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 814 814
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 408 907 1342 1801 3368 408
Size: 499 435 459 1567 1536
Fragments arranged by size:
1567 1536 499 459 435
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 613 847 1500 3849 4105 613
Size: 234 653 2349 256 1004
Fragments arranged by size:
2349 1004 653 256 234
AseI AT'TA_AT
Cuts at: 20 191 1484 20
Size: 171 1293 3032
Fragments arranged by size:
3032 1293 171
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1516 1516
Size: 4496
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 414 619 1512 1926 2174 2209 414
Size: 205 893 414 248 35 2701
Fragments arranged by size:
2701 893 414 248 205 35
BanII G_rGCy'C
Cuts at: 932 1015 1510 2540 932
Size: 83 495 1030 2888
Fragments arranged by size:
2888 1030 495 83
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 346 346
Size: 4496
BbvCI CC'TCA_GC
Cuts at: 776 776
Size: 4496
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 315 349 315
Size: 34 4462
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BfrBI ATG'CAT
Cuts at: 1233 1233
Size: 4496
BglI GCCn_nnn'nGGC
Cuts at: 1347 1347
Size: 4496
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 926 1608 926
Size: 682 3814
Bme1580I G_kGCm'C
Cuts at: 2121 2214 3270 2121
Size: 93 1056 3347
Fragments arranged by size:
3347 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1708 1789 2124 4268 1708
Size: 81 335 2144 1936
Fragments arranged by size:
2144 1936 335 81
BmtI G_CTAG'C
Cuts at: 380 380
Size: 4496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 929 404
Size: 525 3971
Bpu10I CC'TnA_GC
Cuts at: 776 776
Size: 4496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 2551 3043 3341 1672
Size: 879 492 298 2827
Fragments arranged by size:
2827 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 718 1128 4200 718
Size: 410 3072 1014
Fragments arranged by size:
3072 1014 410
BsaAI yAC'GTr
Cuts at: 1849 1849
Size: 4496
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1070 1927 2175 2877 4373 1070
Size: 857 248 702 1496 1193
Fragments arranged by size:
1496 1193 857 702 248
BsaWI w'CCGG_w
Cuts at: 1685 2206 2884 3158 3305 3716 1685
Size: 521 678 274 147 411 2465
Fragments arranged by size:
2465 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 876 1921 2679 3256 3752 876
Size: 1045 758 577 496 1620
Fragments arranged by size:
1620 1045 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 754 421
Size: 333 4163
BsiEI CG_ry'CG
Cuts at: 104 1200 2038 2084 3292 104
Size: 1096 838 46 1208 1308
Fragments arranged by size:
1308 1208 1096 838 46
BsiHKAI G_wGCw'C
Cuts at: 543 1510 2288 2478 3270 543
Size: 967 778 190 792 1769
Fragments arranged by size:
1769 967 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 607 607
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 543 932 1015 1510 2121 2214 2288 2478 2540
Size: 389 83 495 611 93 74 190 62
Cuts at: 2540 3270 543
Size: 730 1769
Fragments arranged by size:
1769 730 611 495 389 190 93 83
74 62
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1229 1546 1760 2062 2443 1229
Size: 317 214 302 381 3282
Fragments arranged by size:
3282 381 317 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 977 2408 977
Size: 1431 3065
BsrFI r'CCGG_y
Cuts at: 862 2494 2675 2884 862
Size: 1632 181 209 2474
Fragments arranged by size:
2474 1632 209 181
BssSI C'ACGA_G
Cuts at: 287 2767 3125 287
Size: 2480 358 1658
Fragments arranged by size:
2480 1658 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1356 1468 1873 1356
Size: 112 405 3979
Fragments arranged by size:
3979 405 112
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstYI r'GATC_y
Cuts at: 1 826 1521 1988 2011 2346 2592 3604 3690
Size: 825 695 467 23 335 246 1012 86
Cuts at: 3690 3702 4048 1
Size: 12 346 449
Fragments arranged by size:
1012 825 695 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2446 2627 2790 2446
Size: 181 163 4152
Fragments arranged by size:
4152 181 163
BtsI GCAGTG_nn'
Cuts at: 1739 1739
Size: 4496
ClaI AT'CG_AT
Cuts at: 674 674
Size: 4496
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 2081 2255 2646 2673 4018 2081
Size: 174 391 27 1345 2559
Fragments arranged by size:
2559 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 122 848 2519 2729 122
Size: 726 1671 210 1889
Fragments arranged by size:
1889 1671 726 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 929 2321 2753 3500 3768 404
Size: 525 1392 432 747 268 1132
Fragments arranged by size:
1392 1132 747 525 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1339 1635 1339
Size: 296 4200
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 240 2515 240
Size: 2275 2221
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1215 1271 1930 2178 3200 4179 1215
Size: 56 659 248 1022 979 1532
Fragments arranged by size:
1532 1022 979 659 248 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 902 934 2338 2368 2370 2400 902
Size: 32 1404 30 2 30 2998
Fragments arranged by size:
2998 1404 32 30 30 2
HincII GTy'rAC
Cuts at: 1535 1535
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 863 3167 3351 863
Size: 2304 184 2008
Fragments arranged by size:
2304 2008 184
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 1109 1238 2485 2612 1109
Size: 129 1247 127 2993
Fragments arranged by size:
2993 1247 129 127
MspA1I CmG'CkG
Cuts at: 755 1607 1921 2281 3294 3539 755
Size: 852 314 360 1013 245 1712
Fragments arranged by size:
1712 1013 852 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NdeI CA'TA_TG
Cuts at: 1171 1171
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NheI G'CTAG_C
Cuts at: 376 376
Size: 4496
NsiI A_TGCA'T
Cuts at: 1235 1235
Size: 4496
NspI r_CATG'y
Cuts at: 795 1114 1187 1237 1549 2049 2956 795
Size: 319 73 50 312 500 907 2335
Fragments arranged by size:
2335 907 500 319 312 73 50
PciI A'CATG_T
Cuts at: 1110 1183 2952 1110
Size: 73 1769 2654
Fragments arranged by size:
2654 1769 73
PflMI CCAn_nnn'nTGG
Cuts at: 1172 1172
Size: 4496
PfoI T'CCnGG_A
Cuts at: 822 822
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PstI C_TGCA'G
Cuts at: 1543 2228 1543
Size: 685 3811
PvuI CG_AT'CG
Cuts at: 104 1200 104
Size: 1096 3400
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 1539 2224 3217 3408 33
Size: 1506 685 993 191 1121
Fragments arranged by size:
1506 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SgrAI Cr'CCGG_yG
Cuts at: 862 862
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1651 2566 3058 3320 1651
Size: 915 492 262 2827
Fragments arranged by size:
2827 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StyI C'CwwG_G
Cuts at: 167 1630 1984 167
Size: 1463 354 2679
Fragments arranged by size:
2679 1463 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1228 2079 2212 2671 2880 1228
Size: 851 133 459 209 2844
Fragments arranged by size:
2844 851 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 174 555 1991 174
Size: 381 1436 2679
Fragments arranged by size:
2679 1436 381
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BmgBI BsaXI BseRI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI