Restriction map for
pF1KE4379
(Circular) (Six-base) MAPSORT of: pF1KE4379.seq Check: 5751 from: 1 to: 4310
pF1KE4379 4310 bp
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AccI GT'mk_AC
Cuts at: 1348 1348
Size: 4310
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 1244 2135 2567 3314 3582 275
Size: 969 891 432 747 268 1003
Fragments arranged by size:
1003 969 891 747 432 268
AflII C'TTAA_G
Cuts at: 457 457
Size: 4310
AflIII A'CryG_T
Cuts at: 1662 2766 3743 1662
Size: 1104 977 2229
Fragments arranged by size:
2229 1104 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 1123 1133 1615 3182 1123
Size: 10 482 1567 2251
Fragments arranged by size:
2251 1567 482 10
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 688 955 1314 3663 3919 688
Size: 267 359 2349 256 1079
Fragments arranged by size:
2349 1079 359 267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 983 1330 983
Size: 347 3963
BamHI G'GATC_C
Cuts at: 908 1335 908
Size: 427 3883
BanI G'GyrC_C
Cuts at: 1326 1740 1988 2023 1326
Size: 414 248 35 3613
Fragments arranged by size:
3613 414 248 35
BanII G_rGCy'C
Cuts at: 779 1324 2354 779
Size: 545 1030 2735
Fragments arranged by size:
2735 1030 545
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BbsI GAAGACnn'nnnn_
Cuts at: 281 542 599 1072 281
Size: 261 57 473 3519
Fragments arranged by size:
3519 473 261 57
BbvCI CC'TCA_GC
Cuts at: 1172 1172
Size: 4310
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 1935 2028 3084 1935
Size: 93 1056 3161
Fragments arranged by size:
3161 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 433 535 1068 1522 1603 1938 4082 433
Size: 102 533 454 81 335 2144 661
Fragments arranged by size:
2144 661 533 454 335 102 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1201 982
Size: 219 4091
Bpu10I CC'TnA_GC
Cuts at: 1172 1172
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1486 2365 2857 3155 358
Size: 1128 879 492 298 1513
Fragments arranged by size:
1513 1128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 1663 1663
Size: 4310
BsaBI GATnn'nnATC
Cuts at: 6 1157 1824 3861 6
Size: 1151 667 2037 455
Fragments arranged by size:
2037 1151 667 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1499 2020 2698 2972 3119 3530 1499
Size: 521 678 274 147 411 2279
Fragments arranged by size:
2279 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 826 1189 826
Size: 363 3947
BseYI C'CCAG_C
Cuts at: 1735 2493 3070 3566 1735
Size: 758 577 496 2479
Fragments arranged by size:
2479 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1324 2102 2292 3084 1324
Size: 778 190 792 2550
Fragments arranged by size:
2550 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 779 1324 1935 2028 2102 2292 2354 3084 779
Size: 545 611 93 74 190 62 730 2005
Fragments arranged by size:
2005 730 611 545 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 228 1360 1574 1876 2257 228
Size: 1132 214 302 381 2281
Fragments arranged by size:
2281 1132 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2602 2656 4140 4305 50
Size: 2552 54 1484 165 55
Fragments arranged by size:
2552 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 779 2222 779
Size: 1443 2867
BsrFI r'CCGG_y
Cuts at: 282 2308 2489 2698 282
Size: 2026 181 209 1894
Fragments arranged by size:
2026 1894 209 181
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 581 2671 581
Size: 2090 2220
BstXI CCAn_nnnn'nTGG
Cuts at: 423 423
Size: 4310
BstYI r'GATC_y
Cuts at: 1 525 908 965 1335 1802 1825 2160 2406
Size: 524 383 57 370 467 23 335 246
Cuts at: 2406 3418 3504 3516 3862 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 524 467 449 383 370 346 335
246 86 57 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 1553 1553
Size: 4310
DraI TTT'AAA
Cuts at: 245 1309 245
Size: 1064 3246
DraIII CAC_nnn'GTG
Cuts at: 918 918
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 1895 2069 2460 2487 3832 1895
Size: 174 391 27 1345 2373
Fragments arranged by size:
2373 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 106 161 803 928 1262 2333 2543 106
Size: 55 642 125 334 1071 210 1873
Fragments arranged by size:
1873 1071 642 334 210 125 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 982 1201 1244 2135 2567 3314 3582 275
Size: 707 219 43 891 432 747 268 1003
Fragments arranged by size:
1003 891 747 707 432 268 219 43
EcoNI CCTnn'n_nnAGG
Cuts at: 156 973 156
Size: 817 3493
EcoO109I rG'GnC_Cy
Cuts at: 354 1449 354
Size: 1095 3215
EcoRI G'AATT_C
Cuts at: 955 1314 955
Size: 359 3951
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 190 427 2329 190
Size: 237 1902 2171
Fragments arranged by size:
2171 1902 237
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 1744 1992 3014 3993 1744
Size: 248 1022 979 2061
Fragments arranged by size:
2061 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 2152 2182 2184 2214 271
Size: 32 1849 30 2 30 2367
Fragments arranged by size:
2367 1849 32 30 30 2
HincII GTy'rAC
Cuts at: 750 1349 750
Size: 599 3711
HindIII A'AGCT_T
Cuts at: 409 409
Size: 4310
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 342 1189 2981 3165 342
Size: 847 1792 184 1487
Fragments arranged by size:
1792 1487 847 184
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 2299 2426 2299
Size: 127 4183
MspA1I CmG'CkG
Cuts at: 499 664 1421 1735 2095 3108 3353 499
Size: 165 757 314 360 1013 245 1456
Fragments arranged by size:
1456 1013 757 360 314 245 165
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NdeI CA'TA_TG
Cuts at: 189 189
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 1363 1863 2770 1363
Size: 500 907 2903
Fragments arranged by size:
2903 907 500
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PflMI CCAn_nnn'nTGG
Cuts at: 918 1099 918
Size: 181 4129
PfoI T'CCnGG_A
Cuts at: 959 959
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 354 354
Size: 4310
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 499 664 1735 2095 499
Size: 165 1071 360 2714
Fragments arranged by size:
2714 1071 360 165
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 371 1353 2038 3031 3222 33
Size: 338 982 685 993 191 1121
Fragments arranged by size:
1121 993 982 685 338 191
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 985 1332 985
Size: 347 3963
SmlI C'TyrA_G
Cuts at: 337 457 1465 2380 2872 3134 337
Size: 120 1008 915 492 262 1513
Fragments arranged by size:
1513 1008 915 492 262 120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
StyI C'CwwG_G
Cuts at: 1444 1798 1444
Size: 354 3956
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 218 1893 2026 2485 2694 218
Size: 1675 133 459 209 1834
Fragments arranged by size:
1834 1675 459 209 133
TatI w'GTAC_w
Cuts at: 3825 3825
Size: 4310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4278
Tth111I GACn'n_nGTC
Cuts at: 1129 2107 1129
Size: 978 3332
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1805 1805
Size: 4310
XmaI C'CCGG_G
Cuts at: 983 1330 983
Size: 347 3963
XmnI GAAnn'nnTTC
Cuts at: 229 1069 229
Size: 840 3470
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI