Restriction map for
pF1KE4378
(Circular) (Six-base) MAPSORT of: pF1KE4378.seq Check: 9548 from: 1 to: 4310
pF1KE4378 4310 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 233 1348 233
Size: 1115 3195
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 963 2135 2567 3314 3582 819
Size: 144 1172 432 747 268 1547
Fragments arranged by size:
1547 1172 747 432 268 144
AflIII A'CryG_T
Cuts at: 580 1662 2766 3743 580
Size: 1082 1104 977 1147
Fragments arranged by size:
1147 1104 1082 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 500 1615 3182 500
Size: 1115 1567 1628
Fragments arranged by size:
1628 1567 1115
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 1314 3663 3919 1314
Size: 2349 256 1705
Fragments arranged by size:
2349 1705 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 279 1330 279
Size: 1051 3259
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 599 1326 1740 1988 2023 599
Size: 727 414 248 35 2886
Fragments arranged by size:
2886 727 414 248 35
BanII G_rGCy'C
Cuts at: 1324 2354 1324
Size: 1030 3280
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BbsI GAAGACnn'nnnn_
Cuts at: 372 497 583 757 786 372
Size: 125 86 174 29 3896
Fragments arranged by size:
3896 174 125 86 29
BbvCI CC'TCA_GC
Cuts at: 1220 1220
Size: 4310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 858 892 858
Size: 34 4276
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglI GCCn_nnn'nGGC
Cuts at: 1185 1185
Size: 4310
BglII A'GATC_T
Cuts at: 587 1825 3862 587
Size: 1238 2037 1035
Fragments arranged by size:
2037 1238 1035
BlpI GC'TnA_GC
Cuts at: 1244 1422 1244
Size: 178 4132
Bme1580I G_kGCm'C
Cuts at: 604 790 1935 2028 3084 604
Size: 186 1145 93 1056 1830
Fragments arranged by size:
1830 1145 1056 186 93
BmgBI CAC'GTC
Cuts at: 581 581
Size: 4310
BmrI ACTGGGnnnn_n'
Cuts at: 784 1522 1603 1938 4082 784
Size: 738 81 335 2144 1012
Fragments arranged by size:
2144 1012 738 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
Bpu10I CC'TnA_GC
Cuts at: 1220 1220
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 431 1486 2365 2857 3155 298
Size: 133 1055 879 492 298 1453
Fragments arranged by size:
1453 1055 879 492 298 133
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 810 906 1663 810
Size: 96 757 3457
Fragments arranged by size:
3457 757 96
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 1741 1989 2691 4187 1741
Size: 248 702 1496 1864
Fragments arranged by size:
1864 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1499 2020 2698 2972 3119 3530 1499
Size: 521 678 274 147 411 2279
Fragments arranged by size:
2279 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 742 142
Size: 600 3710
BseYI C'CCAG_C
Cuts at: 332 384 1735 2493 3070 3566 332
Size: 52 1351 758 577 496 1076
Fragments arranged by size:
1351 1076 758 577 496 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 760
Size: 4310
BsiEI CG_ry'CG
Cuts at: 104 1852 1898 3106 104
Size: 1748 46 1208 1308
Fragments arranged by size:
1748 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 467 1324 2102 2292 3084 467
Size: 857 778 190 792 1693
Fragments arranged by size:
1693 857 792 778 190
BsiWI C'GTAC_G
Cuts at: 906 906
Size: 4310
BsmI GAATG_Cn'
Cuts at: 741 741
Size: 4310
Bsp1286I G_dGCh'C
Cuts at: 467 604 790 1324 1935 2028 2102 2292 2354
Size: 137 186 534 611 93 74 190 62
Cuts at: 2354 3084 467
Size: 730 1693
Fragments arranged by size:
1693 730 611 534 190 186 137 93
74 62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 413 3486 413
Size: 3073 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 763 1166 1360 1574 1876 2257 763
Size: 403 194 214 302 381 2816
Fragments arranged by size:
2816 403 381 302 214 194
BsrBI CCG'CTC
Cuts at: 50 284 2602 2656 4140 4305 50
Size: 234 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 2308 2489 2698 2308
Size: 181 209 3920
Fragments arranged by size:
3920 209 181
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstXI CCAn_nnnn'nTGG
Cuts at: 340 340
Size: 4310
BstYI r'GATC_y
Cuts at: 1 587 1335 1802 1825 2160 2406 3418 3504
Size: 586 748 467 23 335 246 1012 86
Cuts at: 3504 3516 3862 1
Size: 12 346 449
Fragments arranged by size:
1012 748 586 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 605 936 107
Size: 498 331 3481
Fragments arranged by size:
3481 498 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 303 912 1553 303
Size: 609 641 3060
Fragments arranged by size:
3060 641 609
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DraIII CAC_nnn'GTG
Cuts at: 999 999
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 134 323 1139 1895 2069 2460 2487 3832 134
Size: 189 816 756 174 391 27 1345 612
Fragments arranged by size:
1345 816 756 612 391 189 174 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 151 944 1033 2333 2543 151
Size: 793 89 1300 210 1918
Fragments arranged by size:
1918 1300 793 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 963 2135 2567 3314 3582 819
Size: 144 1172 432 747 268 1547
Fragments arranged by size:
1547 1172 747 432 268 144
EcoNI CCTnn'n_nnAGG
Cuts at: 724 1030 724
Size: 306 4004
EcoO109I rG'GnC_Cy
Cuts at: 318 1449 318
Size: 1131 3179
EcoRI G'AATT_C
Cuts at: 1314 1314
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 459 2091 459
Size: 1632 2678
HaeII r_GCGC'y
Cuts at: 1744 1992 3014 3993 1744
Size: 248 1022 979 2061
Fragments arranged by size:
2061 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2152 2182 2184 2214 2152
Size: 30 2 30 4248
Fragments arranged by size:
4248 30 30 2
HincII GTy'rAC
Cuts at: 708 1349 708
Size: 641 3669
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 231 2981 3165 231
Size: 2750 184 1376
Fragments arranged by size:
2750 1376 184
MscI TGG'CCA
Cuts at: 1141 2071 1141
Size: 930 3380
MslI CAynn'nnrTG
Cuts at: 1060 2299 2426 1060
Size: 1239 127 2944
Fragments arranged by size:
2944 1239 127
MspA1I CmG'CkG
Cuts at: 219 1421 1735 2095 3108 3353 219
Size: 1202 314 360 1013 245 1176
Fragments arranged by size:
1202 1176 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NcoI C'CATG_G
Cuts at: 107 936 107
Size: 829 3481
NdeI CA'TA_TG
Cuts at: 481 481
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 1363 1863 2770 1363
Size: 500 907 2903
Fragments arranged by size:
2903 907 500
PasI CC'CwG_GG
Cuts at: 390 508 1275 390
Size: 118 767 3425
Fragments arranged by size:
3425 767 118
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PflMI CCAn_nnn'nTGG
Cuts at: 222 881 222
Size: 659 3651
PfoI T'CCnGG_A
Cuts at: 122 122
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PspXI vC'TCGA_Gb
Cuts at: 279 279
Size: 4310
PstI C_TGCA'G
Cuts at: 919 1286 1357 2042 919
Size: 367 71 685 3187
Fragments arranged by size:
3187 685 367 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 219 1735 2095 219
Size: 1516 360 2434
Fragments arranged by size:
2434 1516 360
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1286 1357 1286
Size: 71 4239
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SexAI A'CCwGG_T
Cuts at: 719 719
Size: 4310
SfcI C'TryA_G
Cuts at: 33 915 1282 1353 2038 3031 3222 33
Size: 882 367 71 685 993 191 1121
Fragments arranged by size:
1121 993 882 685 367 191 71
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 279 313 446 1465 2380 2872 3134 279
Size: 34 133 1019 915 492 262 1455
Fragments arranged by size:
1455 1019 915 492 262 133 34
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
StyI C'CwwG_G
Cuts at: 107 531 936 1444 1798 107
Size: 424 405 508 354 2619
Fragments arranged by size:
2619 508 424 405 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 237 482 1893 2026 2485 2694 237
Size: 245 1411 133 459 209 1853
Fragments arranged by size:
1853 1411 459 245 209 133
TatI w'GTAC_w
Cuts at: 3825 3825
Size: 4310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 104 136 3827 3859 104
Size: 32 3691 32 555
Fragments arranged by size:
3691 555 32 32
Tth111I GACn'n_nGTC
Cuts at: 376 2107 376
Size: 1731 2579
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 996 1272 1805 996
Size: 276 533 3501
Fragments arranged by size:
3501 533 276
XhoI C'TCGA_G
Cuts at: 279 279
Size: 4310
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
XmnI GAAnn'nnTTC
Cuts at: 1207 1207
Size: 4310
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmtI BpmI BsaXI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PmlI PpuMI PshAI PsiI PspOMI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI ZraI