Restriction map for
pF1KE4281
(Circular) (Six-base) MAPSORT of: pF1KE4281.seq Check: 9897 from: 1 to: 7619
pF1KE4281 7619 bp
With 182 enzymes: SgfI *
March 8, 2012 13:22 ..
AccI GT'mk_AC
Cuts at: 4393 4657 4393
Size: 264 7355
Acc65I G'GTAC_C
Cuts at: 4635 4635
Size: 7619
AclI AA'CG_TT
Cuts at: 7459 7459
Size: 7619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 2168 2834 3353 3374 4528 5444 5876 6623
Size: 1590 666 519 21 1154 916 432 747
Cuts at: 6623 6891 578
Size: 268 1306
Fragments arranged by size:
1590 1306 1154 916 747 666 519 432
268 21
AflII C'TTAA_G
Cuts at: 1735 1857 1735
Size: 122 7497
AflIII A'CryG_T
Cuts at: 817 1774 3859 4971 6075 7052 817
Size: 957 2085 1112 1104 977 1384
Fragments arranged by size:
2085 1384 1112 1104 977 957
AgeI A'CCGG_T
Cuts at: 6007 6007
Size: 7619
AleI CACnn'nnGTG
Cuts at: 2076 2076
Size: 7619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1838 1838
Size: 7619
AlwNI CAG_nnn'CTG
Cuts at: 1368 2434 2761 3020 3906 4924 6491 1368
Size: 1066 327 259 886 1018 1567 2496
Fragments arranged by size:
2496 1567 1066 1018 886 327 259
ApaLI G'TGCA_C
Cuts at: 6389 6389
Size: 7619
ApoI r'AATT_y
Cuts at: 374 438 640 1405 1427 1658 1991 2125 2326
Size: 64 202 765 22 231 333 134 201
Cuts at: 2326 2848 2946 3467 3955 4234 4623 6972 7228
Size: 522 98 521 488 279 389 2349 256
Cuts at: 7228 374
Size: 765
Fragments arranged by size:
2349 765 765 522 521 488 389 333
279 256 231 202 201 134 98 64
22
AseI AT'TA_AT
Cuts at: 20 1292 2200 20
Size: 1272 908 5439
Fragments arranged by size:
5439 1272 908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7619
AvaI C'yCGr_G
Cuts at: 3332 4639 3332
Size: 1307 6312
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 826 859 1860 1893 826
Size: 33 1001 33 6552
Fragments arranged by size:
6552 1001 33 33
BamHI G'GATC_C
Cuts at: 3363 4596 4644 3363
Size: 1233 48 6338
Fragments arranged by size:
6338 1233 48
BanI G'GyrC_C
Cuts at: 2252 3336 4536 4635 5049 5297 5332 2252
Size: 1084 1200 99 414 248 35 4539
Fragments arranged by size:
4539 1200 1084 414 248 99 35
BanII G_rGCy'C
Cuts at: 2404 4107 4633 5663 2404
Size: 1703 526 1030 4360
Fragments arranged by size:
4360 1703 1030 526
BbeI G_GCGC'C
Cuts at: 5053 5301 5053
Size: 248 7371
BbsI GAAGACnn'nnnn_
Cuts at: 3684 4198 3684
Size: 514 7105
BbvCI CC'TCA_GC
Cuts at: 4568 4568
Size: 7619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2682 2716 2682
Size: 34 7585
BciVI GTATCCnnnnn_n'
Cuts at: 3173 5517 6278 7619 3173
Size: 2344 761 1341 3173
Fragments arranged by size:
3173 2344 1341 761
BclI T'GATC_A
Cuts at: 315 513 630 4044 5139 315
Size: 198 117 3414 1095 2795
Fragments arranged by size:
3414 2795 1095 198 117
BfrBI ATG'CAT
Cuts at: 593 593
Size: 7619
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 7619
BglII A'GATC_T
Cuts at: 1620 4125 5134 7171 1620
Size: 2505 1009 2037 2068
Fragments arranged by size:
2505 2068 2037 1009
BlpI GC'TnA_GC
Cuts at: 277 4731 277
Size: 4454 3165
Bme1580I G_kGCm'C
Cuts at: 5244 5337 6393 5244
Size: 93 1056 6470
Fragments arranged by size:
6470 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 339 4831 4912 5247 7391 339
Size: 4492 81 335 2144 567
Fragments arranged by size:
4492 2144 567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5986 5986
Size: 7619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4121 3883
Size: 238 7381
Bpu10I CC'TnA_GC
Cuts at: 1321 4568 1321
Size: 3247 4372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2460 2516 4795 5674 6166 6464 2460
Size: 56 2279 879 492 298 3615
Fragments arranged by size:
3615 2279 879 492 298 56
BsaI GGTCTCn'nnnn_
Cuts at: 4356 7323 4356
Size: 2967 4652
BsaAI yAC'GTr
Cuts at: 3862 4269 4972 3862
Size: 407 703 6509
Fragments arranged by size:
6509 703 407
BsaBI GATnn'nnATC
Cuts at: 6 728 1619 3362 4049 5133 7170 6
Size: 722 891 1743 687 1084 2037 455
Fragments arranged by size:
2037 1743 1084 891 722 687 455
BsaHI Gr'CG_yC
Cuts at: 5050 5298 6000 7496 5050
Size: 248 702 1496 5173
Fragments arranged by size:
5173 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4808 5329 6007 6281 6428 6839 4808
Size: 521 678 274 147 411 5588
Fragments arranged by size:
5588 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 4454 4484 287
Size: 30 4137 30 3422
Fragments arranged by size:
4137 3422 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1398 2375 4484 1398
Size: 977 2109 4533
Fragments arranged by size:
4533 2109 977
BseYI C'CCAG_C
Cuts at: 5044 5802 6379 6875 5044
Size: 758 577 496 5788
Fragments arranged by size:
5788 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 812 967 482
Size: 330 155 7134
Fragments arranged by size:
7134 330 155
BsiEI CG_ry'CG
Cuts at: 104 2014 5161 5207 6415 104
Size: 1910 3147 46 1208 1308
Fragments arranged by size:
3147 1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1327 4633 5411 5601 6393 1327
Size: 3306 778 190 792 2553
Fragments arranged by size:
3306 2553 792 778 190
BsmI GAATG_Cn'
Cuts at: 373 721 1596 2705 373
Size: 348 875 1109 5287
Fragments arranged by size:
5287 1109 875 348
Bsp1286I G_dGCh'C
Cuts at: 1327 2404 4107 4633 5244 5337 5411 5601 5663
Size: 1077 1703 526 611 93 74 190 62
Cuts at: 5663 6393 1327
Size: 730 2553
Fragments arranged by size:
2553 1703 1077 730 611 526 190 93
74 62
BspEI T'CCGG_A
Cuts at: 6839 6839
Size: 7619
BspHI T'CATG_A
Cuts at: 3189 6795 3189
Size: 3606 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2313 4669 4883 5185 5566 2313
Size: 2356 214 302 381 4366
Fragments arranged by size:
4366 2356 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5911 5965 7449 7614 50
Size: 5861 54 1484 165 55
Fragments arranged by size:
5861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2290 2825 4558 5531 2290
Size: 535 1733 973 4378
Fragments arranged by size:
4378 1733 973 535
BsrFI r'CCGG_y
Cuts at: 2013 5617 5798 6007 2013
Size: 3604 181 209 3625
Fragments arranged by size:
3625 3604 209 181
BsrGI T'GTAC_A
Cuts at: 500 1359 1795 2742 3099 500
Size: 859 436 947 357 5020
Fragments arranged by size:
5020 947 859 436 357
BssSI C'ACGA_G
Cuts at: 5890 6248 5890
Size: 358 7261
BstAPI GCAn_nnn'nTGC
Cuts at: 4996 4996
Size: 7619
BstBI TT'CG_AA
Cuts at: 3471 5980 3471
Size: 2509 5110
BstYI r'GATC_y
Cuts at: 1 1620 2469 2484 3363 3699 3903 4125 4134
Size: 1619 849 15 879 336 204 222 9
Cuts at: 4134 4596 4644 5111 5134 5469 5715 6727 6813
Size: 462 48 467 23 335 246 1012 86
Cuts at: 6813 6825 7171 1
Size: 12 346 449
Fragments arranged by size:
1619 1012 879 849 467 462 449 346
336 335 246 222 204 86 48 23
15 12 9
BtgI C'CryG_G
Cuts at: 1453 1453
Size: 7619
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5569 5750 5913 5569
Size: 181 163 7275
Fragments arranged by size:
7275 181 163
BtsI GCAGTG_nn'
Cuts at: 2529 2840 4444 4862 2529
Size: 311 1604 418 5286
Fragments arranged by size:
5286 1604 418 311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4469 4504 4529 4564 4469
Size: 35 25 35 7524
Fragments arranged by size:
7524 35 35 25
DraI TTT'AAA
Cuts at: 2067 3284 4618 2067
Size: 1217 1334 5068
Fragments arranged by size:
5068 1334 1217
DraIII CAC_nnn'GTG
Cuts at: 3121 3807 3121
Size: 686 6933
DrdI GACnn_nn'nnGTC
Cuts at: 5325 6183 5325
Size: 858 6761
EaeI y'GGCC_r
Cuts at: 1785 1851 2011 4348 5204 5378 5769 5796 7141
Size: 66 160 2337 856 174 391 27 1345
Cuts at: 7141 1785
Size: 2263
Fragments arranged by size:
2337 2263 1345 856 391 174 160 66
27
EagI C'GGCC_G
Cuts at: 2011 5204 2011
Size: 3193 4426
EarI CTCTTCn'nnn_
Cuts at: 1109 1163 1375 1565 2625 4160 5642 5852 1109
Size: 54 212 190 1060 1535 1482 210 2876
Fragments arranged by size:
2876 1535 1482 1060 212 210 190 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1317 5827 6137 6283 7429 1317
Size: 4510 310 146 1146 1507
Fragments arranged by size:
4510 1507 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4631 4631
Size: 7619
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 578 2168 2834 3353 3374 3883 4121 4528 5444
Size: 1590 666 519 21 509 238 407 916
Cuts at: 5444 5876 6623 6891 578
Size: 432 747 268 1306
Fragments arranged by size:
1590 1306 916 747 666 519 509 432
407 268 238 21
EcoO109I rG'GnC_Cy
Cuts at: 4758 4758
Size: 7619
EcoRI G'AATT_C
Cuts at: 4623 4623
Size: 7619
EcoRV GAT'ATC
Cuts at: 1373 1373
Size: 7619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1603 1915 3721 5638 1603
Size: 312 1806 1917 3584
Fragments arranged by size:
3584 1917 1806 312
FspI TGC'GCA
Cuts at: 5400 5400
Size: 7619
HaeII r_GCGC'y
Cuts at: 5053 5301 6323 7302 5053
Size: 248 1022 979 5370
Fragments arranged by size:
5370 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 788 820 948 980 1708 1740 2011 2043 3514
Size: 32 128 32 728 32 271 32 1471
Cuts at: 3514 3546 4263 4295 4318 4350 4375 4407 5461
Size: 32 717 32 23 32 25 32 1054
Cuts at: 5461 5491 5493 5523 788
Size: 30 2 30 2884
Fragments arranged by size:
2884 1471 1054 728 717 271 128 32
32 32 32 32 32 32 32 30
30 25 23 2
HincII GTy'rAC
Cuts at: 807 1896 4658 807
Size: 1089 2762 3768
Fragments arranged by size:
3768 2762 1089
HindIII A'AGCT_T
Cuts at: 1196 1694 2150 2687 4112 1196
Size: 498 456 537 1425 4703
Fragments arranged by size:
4703 1425 537 498 456
HpaI GTT'AAC
Cuts at: 1896 1896
Size: 7619
KasI G'GCGC_C
Cuts at: 5049 5297 5049
Size: 248 7371
KpnI G_GTAC'C
Cuts at: 4639 4639
Size: 7619
MfeI C'AATT_G
Cuts at: 2624 3086 3290 2624
Size: 462 204 6953
Fragments arranged by size:
6953 462 204
MluI A'CGCG_T
Cuts at: 7052 7052
Size: 7619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 141 183 824 1823 3136 6290 6474 141
Size: 42 641 999 1313 3154 184 1286
Fragments arranged by size:
3154 1313 1286 999 641 184 42
MscI TGG'CCA
Cuts at: 1787 4350 5380 1787
Size: 2563 1030 4026
Fragments arranged by size:
4026 2563 1030
MslI CAynn'nnrTG
Cuts at: 1807 2076 2987 3194 3626 5608 5735 1807
Size: 269 911 207 432 1982 127 3691
Fragments arranged by size:
3691 1982 911 432 269 207 127
MspA1I CmG'CkG
Cuts at: 466 2748 3916 4004 4572 4730 5044 5404 6417
Size: 2282 1168 88 568 158 314 360 1013
Cuts at: 6417 6662 466
Size: 245 1423
Fragments arranged by size:
2282 1423 1168 1013 568 360 314 245
158 88
NaeI GCC'GGC
Cuts at: 5800 5800
Size: 7619
NarI GG'CG_CC
Cuts at: 5050 5298 5050
Size: 248 7371
NcoI C'CATG_G
Cuts at: 1453 1453
Size: 7619
NgoMIV G'CCGG_C
Cuts at: 5798 5798
Size: 7619
NsiI A_TGCA'T
Cuts at: 595 595
Size: 7619
NspI r_CATG'y
Cuts at: 821 1778 4672 5172 6079 821
Size: 957 2894 500 907 2361
Fragments arranged by size:
2894 2361 957 907 500
PciI A'CATG_T
Cuts at: 817 1774 6075 817
Size: 957 4301 2361
Fragments arranged by size:
4301 2361 957
PflMI CCAn_nnn'nTGG
Cuts at: 3906 3906
Size: 7619
PfoI T'CCnGG_A
Cuts at: 3899 4187 3899
Size: 288 7331
PmeI GTTT'AAAC
Cuts at: 4618 4618
Size: 7619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6758 6790 6758
Size: 32 7587
PsiI TTA'TAA
Cuts at: 893 893
Size: 7619
PstI C_TGCA'G
Cuts at: 4666 5351 4666
Size: 685 6934
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7619
PvuII CAG'CTG
Cuts at: 466 2748 3916 4572 5044 5404 466
Size: 2282 1168 656 472 360 2681
Fragments arranged by size:
2681 2282 1168 656 472 360
RsrII CG'GwC_CG
Cuts at: 5814 5814
Size: 7619
SacI G_AGCT'C
Cuts at: 4633 4633
Size: 7619
SalI G'TCGA_C
Cuts at: 4656 4656
Size: 7619
SapI GCTCTTCn'nnn_
Cuts at: 5642 5852 5642
Size: 210 7409
SbfI CC_TGCA'GG
Cuts at: 4666 4666
Size: 7619
ScaI AGT'ACT
Cuts at: 7136 7136
Size: 7619
SfcI C'TryA_G
Cuts at: 33 3609 4094 4662 5347 6340 6531 33
Size: 3576 485 568 685 993 191 1121
Fragments arranged by size:
3576 1121 993 685 568 485 191
SfoI GGC'GCC
Cuts at: 5051 5299 5051
Size: 248 7371
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7619
SmaI CCC'GGG
Cuts at: 3334 4641 3334
Size: 1307 6312
SmlI C'TyrA_G
Cuts at: 1735 1857 2439 2531 4774 5689 6181 6443 1735
Size: 122 582 92 2243 915 492 262 2911
Fragments arranged by size:
2911 2243 915 582 492 262 122 92
SpeI A'CTAG_T
Cuts at: 67 2646 67
Size: 2579 5040
SphI G_CATG'C
Cuts at: 4672 5172 4672
Size: 500 7119
SspI AAT'ATT
Cuts at: 253 2606 4029 253
Size: 2353 1423 3843
Fragments arranged by size:
3843 2353 1423
StuI AGG'CCT
Cuts at: 3555 3555
Size: 7619
StyI C'CwwG_G
Cuts at: 1261 1453 2256 3495 4753 5107 1261
Size: 192 803 1239 1258 354 3773
Fragments arranged by size:
3773 1258 1239 803 354 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2066 3508 5202 5335 5794 6003 2066
Size: 1442 1694 133 459 209 3682
Fragments arranged by size:
3682 1694 1442 459 209 133
TatI w'GTAC_w
Cuts at: 326 500 1359 1795 2742 3099 7134 326
Size: 174 859 436 947 357 4035 811
Fragments arranged by size:
4035 947 859 811 436 357 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1453 1485 4508 4540 7136 7168 1453
Size: 32 3023 32 2596 32 1904
Fragments arranged by size:
3023 2596 1904 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5416 5416
Size: 7619
XbaI T'CTAG_A
Cuts at: 1164 2922 4650 1164
Size: 1758 1728 4133
Fragments arranged by size:
4133 1758 1728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1451 2623 5114 1451
Size: 1172 2491 3956
Fragments arranged by size:
3956 2491 1172
XmaI C'CCGG_G
Cuts at: 3332 4639 3332
Size: 1307 6312
XmnI GAAnn'nnTTC
Cuts at: 1427 1574 1727 2648 3621 4205 1427
Size: 147 153 921 973 584 4841
Fragments arranged by size:
4841 973 921 584 153 147
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BmgBI BmtI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI EcoNI FseI FspAI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI