Restriction map for
pF1KE4271
(Circular) (Six-base) MAPSORT of: pF1KE4271.seq Check: 3690 from: 1 to: 6524
pF1KE4271 6524 bp
With 182 enzymes: SgfI *
March 15, 2013 17:10 ..
AccI GT'mk_AC
Cuts at: 196 960 2397 3562 196
Size: 764 1437 1165 3158
Fragments arranged by size:
3158 1437 1165 764
Acc65I G'GTAC_C
Cuts at: 3540 3540
Size: 6524
AclI AA'CG_TT
Cuts at: 6364 6364
Size: 6524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 677 4349 4781 5528 5796 677
Size: 3672 432 747 268 1405
Fragments arranged by size:
3672 1405 747 432 268
AflIII A'CryG_T
Cuts at: 3876 4980 5957 3876
Size: 1104 977 4443
Fragments arranged by size:
4443 1104 977
AgeI A'CCGG_T
Cuts at: 4912 4912
Size: 6524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 857 857
Size: 6524
AlwNI CAG_nnn'CTG
Cuts at: 160 268 3829 5396 160
Size: 108 3561 1567 1288
Fragments arranged by size:
3561 1567 1288 108
ApaLI G'TGCA_C
Cuts at: 5294 5294
Size: 6524
ApoI r'AATT_y
Cuts at: 523 741 1463 1642 1677 1705 2059 2129 2261
Size: 218 722 179 35 28 354 70 132
Cuts at: 2261 2545 3350 3528 5877 6133 523
Size: 284 805 178 2349 256 914
Fragments arranged by size:
2349 914 805 722 354 284 256 218
179 178 132 70 35 28
AseI AT'TA_AT
Cuts at: 20 677 1718 3394 20
Size: 657 1041 1676 3150
Fragments arranged by size:
3150 1676 1041 657
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
AvaI C'yCGr_G
Cuts at: 908 3544 908
Size: 2636 3888
BamHI G'GATC_C
Cuts at: 3231 3549 3231
Size: 318 6206
BanI G'GyrC_C
Cuts at: 2696 3540 3954 4202 4237 2696
Size: 844 414 248 35 4983
Fragments arranged by size:
4983 844 414 248 35
BanII G_rGCy'C
Cuts at: 917 2818 3538 4568 917
Size: 1901 720 1030 2873
Fragments arranged by size:
2873 1901 1030 720
BbeI G_GCGC'C
Cuts at: 3958 4206 3958
Size: 248 6276
BbsI GAAGACnn'nnnn_
Cuts at: 619 619
Size: 6524
BciVI GTATCCnnnnn_n'
Cuts at: 4422 5183 6524 4422
Size: 761 1341 4422
Fragments arranged by size:
4422 1341 761
BclI T'GATC_A
Cuts at: 4044 4044
Size: 6524
BfrBI ATG'CAT
Cuts at: 2292 3447 2292
Size: 1155 5369
BglI GCCn_nnn'nGGC
Cuts at: 283 283
Size: 6524
BglII A'GATC_T
Cuts at: 4039 6076 4039
Size: 2037 4487
BlpI GC'TnA_GC
Cuts at: 153 2630 3636 153
Size: 2477 1006 3041
Fragments arranged by size:
3041 2477 1006
Bme1580I G_kGCm'C
Cuts at: 4149 4242 5298 4149
Size: 93 1056 5375
Fragments arranged by size:
5375 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3736 3817 4152 6296 3736
Size: 81 335 2144 3964
Fragments arranged by size:
3964 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4891 4891
Size: 6524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 3365 132
Size: 3233 3291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3109 3403 3700 4579 5071 5369 3109
Size: 294 297 879 492 298 4264
Fragments arranged by size:
4264 879 492 298 297 294
BsaI GGTCTCn'nnnn_
Cuts at: 366 6228 366
Size: 5862 662
BsaAI yAC'GTr
Cuts at: 342 1954 3877 342
Size: 1612 1923 2989
Fragments arranged by size:
2989 1923 1612
BsaBI GATnn'nnATC
Cuts at: 6 1139 2845 4038 6075 6
Size: 1133 1706 1193 2037 455
Fragments arranged by size:
2037 1706 1193 1133 455
BsaHI Gr'CG_yC
Cuts at: 3955 4203 4905 6401 3955
Size: 248 702 1496 4078
Fragments arranged by size:
4078 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2111 2791 3713 4234 4912 5186 5333 5744 2111
Size: 680 922 521 678 274 147 411 2891
Fragments arranged by size:
2891 922 680 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1121 1151 1121
Size: 30 6494
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 210 213 2131 3493 210
Size: 3 1918 1362 3241
Fragments arranged by size:
3241 1918 1362 3
BseYI C'CCAG_C
Cuts at: 297 306 326 1850 2718 3949 4707 5284 5780
Size: 9 20 1524 868 1231 758 577 496
Cuts at: 5780 297
Size: 1041
Fragments arranged by size:
1524 1231 1041 868 758 577 496 20
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 1306 1471 2315 254
Size: 1052 165 844 4463
Fragments arranged by size:
4463 1052 844 165
BsiEI CG_ry'CG
Cuts at: 104 278 287 4066 4112 5320 104
Size: 174 9 3779 46 1208 1308
Fragments arranged by size:
3779 1308 1208 174 46 9
BsiHKAI G_wGCw'C
Cuts at: 917 2818 3055 3538 4316 4506 5298 917
Size: 1901 237 483 778 190 792 2143
Fragments arranged by size:
2143 1901 792 778 483 237 190
BsmI GAATG_Cn'
Cuts at: 648 2294 648
Size: 1646 4878
BsmBI CGTCTCn'nnnn_
Cuts at: 1818 1818
Size: 6524
Bsp1286I G_dGCh'C
Cuts at: 917 2818 3055 3538 4149 4242 4316 4506 4568
Size: 1901 237 483 611 93 74 190 62
Cuts at: 4568 5298 917
Size: 730 2143
Fragments arranged by size:
2143 1901 730 611 483 237 190 93
74 62
BspEI T'CCGG_A
Cuts at: 2111 5744 2111
Size: 3633 2891
BspHI T'CATG_A
Cuts at: 735 3057 5700 735
Size: 2322 2643 1559
Fragments arranged by size:
2643 2322 1559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2085 3470 3574 3788 4090 4471 2085
Size: 1385 104 214 302 381 4138
Fragments arranged by size:
4138 1385 381 302 214 104
BsrBI CCG'CTC
Cuts at: 50 4816 4870 6354 6519 50
Size: 4766 54 1484 165 55
Fragments arranged by size:
4766 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3071 4436 3071
Size: 1365 5159
BsrFI r'CCGG_y
Cuts at: 4522 4703 4912 4522
Size: 181 209 6134
Fragments arranged by size:
6134 209 181
BsrGI T'GTAC_A
Cuts at: 2658 2658
Size: 6524
BssSI C'ACGA_G
Cuts at: 4795 5153 4795
Size: 358 6166
BstAPI GCAn_nnn'nTGC
Cuts at: 268 643 901 3901 268
Size: 375 258 3000 2891
Fragments arranged by size:
3000 2891 375 258
BstBI TT'CG_AA
Cuts at: 664 4885 664
Size: 4221 2303
BstEII G'GTnAC_C
Cuts at: 1845 1845
Size: 6524
BstXI CCAn_nnnn'nTGG
Cuts at: 2408 2408
Size: 6524
BstYI r'GATC_y
Cuts at: 1 2282 3231 3549 4016 4039 4374 4620 5632
Size: 2281 949 318 467 23 335 246 1012
Cuts at: 5632 5718 5730 6076 1
Size: 86 12 346 449
Fragments arranged by size:
2281 1012 949 467 449 346 335 318
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 961 2398 961
Size: 1437 5087
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4474 4655 4818 4474
Size: 181 163 6180
Fragments arranged by size:
6180 181 163
BtsI GCAGTG_nn'
Cuts at: 2523 2585 3767 2523
Size: 62 1182 5280
Fragments arranged by size:
5280 1182 62
DraI TTT'AAA
Cuts at: 1683 2217 3523 1683
Size: 534 1306 4684
Fragments arranged by size:
4684 1306 534
DraIII CAC_nnn'GTG
Cuts at: 2703 3180 2703
Size: 477 6047
DrdI GACnn_nn'nnGTC
Cuts at: 4230 5088 4230
Size: 858 5666
EaeI y'GGCC_r
Cuts at: 275 284 2618 4109 4283 4674 4701 6046 275
Size: 9 2334 1491 174 391 27 1345 753
Fragments arranged by size:
2334 1491 1345 753 391 174 27 9
EagI C'GGCC_G
Cuts at: 275 284 4109 275
Size: 9 3825 2690
Fragments arranged by size:
3825 2690 9
EarI CTCTTCn'nnn_
Cuts at: 364 3036 3515 4547 4757 364
Size: 2672 479 1032 210 2131
Fragments arranged by size:
2672 2131 1032 479 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3131 4732 5042 5188 6334 3131
Size: 1601 310 146 1146 3321
Fragments arranged by size:
3321 1601 1146 310 146
EcoICRI GAG'CTC
Cuts at: 915 2816 3536 915
Size: 1901 720 3903
Fragments arranged by size:
3903 1901 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 677 3365 4349 4781 5528 5796 132
Size: 545 2688 984 432 747 268 860
Fragments arranged by size:
2688 984 860 747 545 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1295 2620 1295
Size: 1325 5199
EcoO109I rG'GnC_Cy
Cuts at: 3663 3663
Size: 6524
EcoRI G'AATT_C
Cuts at: 523 1463 1642 3528 523
Size: 940 179 1886 3519
Fragments arranged by size:
3519 1886 940 179
EcoRV GAT'ATC
Cuts at: 621 621
Size: 6524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4543 4543
Size: 6524
FspI TGC'GCA
Cuts at: 4305 4305
Size: 6524
HaeII r_GCGC'y
Cuts at: 260 295 304 1423 3958 4206 5228 6207 260
Size: 35 9 1119 2535 248 1022 979 577
Fragments arranged by size:
2535 1119 1022 979 577 248 35 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 555 587 1882 1914 1929 1961 3039 3071 3097
Size: 32 1295 32 15 32 1078 32 26
Cuts at: 3097 3129 3191 3223 4366 4396 4398 4428 555
Size: 32 62 32 1143 30 2 30 2651
Fragments arranged by size:
2651 1295 1143 1078 62 32 32 32
32 32 32 30 30 26 15 2
HincII GTy'rAC
Cuts at: 1551 1587 2601 3563 1551
Size: 36 1014 962 4512
Fragments arranged by size:
4512 1014 962 36
HpaI GTT'AAC
Cuts at: 1587 1587
Size: 6524
KasI G'GCGC_C
Cuts at: 3954 4202 3954
Size: 248 6276
KpnI G_GTAC'C
Cuts at: 3544 3544
Size: 6524
MfeI C'AATT_G
Cuts at: 2891 2891
Size: 6524
MluI A'CGCG_T
Cuts at: 5957 5957
Size: 6524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 444 2400 5195 5379 444
Size: 1956 2795 184 1589
Fragments arranged by size:
2795 1956 1589 184
MscI TGG'CCA
Cuts at: 2620 4285 2620
Size: 1665 4859
MslI CAynn'nnrTG
Cuts at: 820 839 2920 4513 4640 820
Size: 19 2081 1593 127 2704
Fragments arranged by size:
2704 2081 1593 127 19
MspA1I CmG'CkG
Cuts at: 265 274 283 330 3635 3949 4309 5322 5567
Size: 9 9 47 3305 314 360 1013 245
Cuts at: 5567 265
Size: 1222
Fragments arranged by size:
3305 1222 1013 360 314 245 47 9
9
NaeI GCC'GGC
Cuts at: 4705 4705
Size: 6524
NarI GG'CG_CC
Cuts at: 3955 4203 3955
Size: 248 6276
NgoMIV G'CCGG_C
Cuts at: 4703 4703
Size: 6524
NotI GC'GGCC_GC
Cuts at: 275 284 275
Size: 9 6515
NsiI A_TGCA'T
Cuts at: 2294 3449 2294
Size: 1155 5369
NspI r_CATG'y
Cuts at: 3577 4077 4984 3577
Size: 500 907 5117
Fragments arranged by size:
5117 907 500
PciI A'CATG_T
Cuts at: 4980 4980
Size: 6524
PfoI T'CCnGG_A
Cuts at: 1087 1322 2492 3342 1087
Size: 235 1170 850 4269
Fragments arranged by size:
4269 1170 850 235
PmeI GTTT'AAAC
Cuts at: 3523 3523
Size: 6524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5663 5695 5663
Size: 32 6492
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1889 1921 1889
Size: 32 6492
PstI C_TGCA'G
Cuts at: 264 273 578 1047 1373 2518 2650 2880 2934
Size: 9 305 469 326 1145 132 230 54
Cuts at: 2934 3467 3571 4256 264
Size: 533 104 685 2532
Fragments arranged by size:
2532 1145 685 533 469 326 305 230
132 104 54 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6524
PvuII CAG'CTG
Cuts at: 330 3949 4309 330
Size: 3619 360 2545
Fragments arranged by size:
3619 2545 360
RsrII CG'GwC_CG
Cuts at: 2114 4719 2114
Size: 2605 3919
SacI G_AGCT'C
Cuts at: 917 2818 3538 917
Size: 1901 720 3903
Fragments arranged by size:
3903 1901 720
SalI G'TCGA_C
Cuts at: 3561 3561
Size: 6524
SapI GCTCTTCn'nnn_
Cuts at: 4547 4757 4547
Size: 210 6314
SbfI CC_TGCA'GG
Cuts at: 3571 3571
Size: 6524
ScaI AGT'ACT
Cuts at: 6041 6041
Size: 6524
SexAI A'CCwGG_T
Cuts at: 242 3176 242
Size: 2934 3590
SfcI C'TryA_G
Cuts at: 33 260 269 347 574 1043 1369 1750 1983
Size: 227 9 78 227 469 326 381 233
Cuts at: 1983 2514 2646 2876 2930 3463 3567 4252 5245
Size: 531 132 230 54 533 104 685 993
Cuts at: 5245 5436 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 533 531 469 381 326
233 230 227 227 191 132 104 78
54 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 283 283
Size: 6524
SfoI GGC'GCC
Cuts at: 3956 4204 3956
Size: 248 6276
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
SmaI CCC'GGG
Cuts at: 3546 3546
Size: 6524
SmlI C'TyrA_G
Cuts at: 3088 3418 3679 4594 5086 5348 3088
Size: 330 261 915 492 262 4264
Fragments arranged by size:
4264 915 492 330 262 261
SnaBI TAC'GTA
Cuts at: 1954 1954
Size: 6524
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6524
SphI G_CATG'C
Cuts at: 3577 4077 3577
Size: 500 6024
SspI AAT'ATT
Cuts at: 921 921
Size: 6524
StyI C'CwwG_G
Cuts at: 2621 3017 3658 4012 2621
Size: 396 641 354 5133
Fragments arranged by size:
5133 641 396 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1833 2794 2914 3148 4107 4240 4699 4908 1833
Size: 961 120 234 959 133 459 209 3449
Fragments arranged by size:
3449 961 959 459 234 209 133 120
TatI w'GTAC_w
Cuts at: 604 2658 2956 6039 604
Size: 2054 298 3083 1089
Fragments arranged by size:
3083 2054 1089 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1001 1033 1121 1153 3123 3155 3305 3337 6041
Size: 32 88 32 1970 32 150 32 2704
Cuts at: 6041 6073 1001
Size: 32 1452
Fragments arranged by size:
2704 1970 1452 150 88 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4321 4321
Size: 6524
XbaI T'CTAG_A
Cuts at: 2538 3555 2538
Size: 1017 5507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 250 4019 250
Size: 3769 2755
XmaI C'CCGG_G
Cuts at: 3544 3544
Size: 6524
XmnI GAAnn'nnTTC
Cuts at: 523 523
Size: 6524
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NsiI NspI PciI PfoI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsiWI BssHII Bsu36I BtgI ClaI CspCI FseI FspAI
HindIII NcoI NdeI NheI NruI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SgrAI SrfI StuI SwaI XhoI ZraI