Restriction map for
pF1KE4227
(Circular) (Six-base) MAPSORT of: pF1KE4227.seq Check: 1394 from: 1 to: 5519
pF1KE4227 5519 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 132 2052 132
Size: 1920 3599
AccI GT'mk_AC
Cuts at: 1502 1967 2557 1502
Size: 465 590 4464
Fragments arranged by size:
4464 590 465
Acc65I G'GTAC_C
Cuts at: 2535 2535
Size: 5519
AclI AA'CG_TT
Cuts at: 5359 5359
Size: 5519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1437 1717 1739 1966 2101 3344 3776 4523
Size: 1190 280 22 227 135 1243 432 747
Cuts at: 4523 4791 247
Size: 268 975
Fragments arranged by size:
1243 1190 975 747 432 280 268 227
135 22
AfeI AGC'GCT
Cuts at: 1141 1141
Size: 5519
AflIII A'CryG_T
Cuts at: 2105 2871 3975 4952 2105
Size: 766 1104 977 2672
Fragments arranged by size:
2672 1104 977 766
AgeI A'CCGG_T
Cuts at: 3907 3907
Size: 5519
AhdI GACnn_n'nnGTC
Cuts at: 852 2331 852
Size: 1479 4040
AleI CACnn'nnGTG
Cuts at: 661 661
Size: 5519
AlwNI CAG_nnn'CTG
Cuts at: 299 618 868 1365 1419 1431 1554 1623 2154
Size: 319 250 497 54 12 123 69 531
Cuts at: 2154 2824 4391 299
Size: 670 1567 1427
Fragments arranged by size:
1567 1427 670 531 497 319 250 123
69 54 12
ApaI G_GGCC'C
Cuts at: 1057 2166 1057
Size: 1109 4410
ApaLI G'TGCA_C
Cuts at: 4289 4289
Size: 5519
ApoI r'AATT_y
Cuts at: 747 2523 4872 5128 747
Size: 1776 2349 256 1138
Fragments arranged by size:
2349 1776 1138 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5519
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
AvaI C'yCGr_G
Cuts at: 234 565 659 758 794 1056 1381 2539 234
Size: 331 94 99 36 262 325 1158 3214
Fragments arranged by size:
3214 1158 331 325 262 99 94 36
BamHI G'GATC_C
Cuts at: 2544 2544
Size: 5519
BanI G'GyrC_C
Cuts at: 427 781 956 1914 2403 2535 2949 3197 3232
Size: 354 175 958 489 132 414 248 35
Cuts at: 3232 427
Size: 2714
Fragments arranged by size:
2714 958 489 414 354 248 175 132
35
BanII G_rGCy'C
Cuts at: 241 288 635 902 1057 1717 2166 2533 3563
Size: 47 347 267 155 660 449 367 1030
Cuts at: 3563 241
Size: 2197
Fragments arranged by size:
2197 1030 660 449 367 347 267 155
47
BbeI G_GCGC'C
Cuts at: 960 2953 3201 960
Size: 1993 248 3278
Fragments arranged by size:
3278 1993 248
BbsI GAAGACnn'nnnn_
Cuts at: 573 1727 1733 2326 573
Size: 1154 6 593 3766
Fragments arranged by size:
3766 1154 593 6
BbvCI CC'TCA_GC
Cuts at: 2437 2437
Size: 5519
BciVI GTATCCnnnnn_n'
Cuts at: 3417 4178 5519 3417
Size: 761 1341 3417
Fragments arranged by size:
3417 1341 761
BclI T'GATC_A
Cuts at: 1259 3039 1259
Size: 1780 3739
BglI GCCn_nnn'nGGC
Cuts at: 1741 2050 1741
Size: 309 5210
BglII A'GATC_T
Cuts at: 3034 5071 3034
Size: 2037 3482
BlpI GC'TnA_GC
Cuts at: 1216 1330 2054 2631 1216
Size: 114 724 577 4104
Fragments arranged by size:
4104 724 577 114
Bme1580I G_kGCm'C
Cuts at: 432 765 1057 2166 2209 2408 3144 3237 4293
Size: 333 292 1109 43 199 736 93 1056
Cuts at: 4293 432
Size: 1658
Fragments arranged by size:
1658 1109 1056 736 333 292 199 93
43
BmgBI CAC'GTC
Cuts at: 1008 1467 1008
Size: 459 5060
BmrI ACTGGGnnnn_n'
Cuts at: 158 235 2506 2731 2812 3147 5291 158
Size: 77 2271 225 81 335 2144 386
Fragments arranged by size:
2271 2144 386 335 225 81 77
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 685 3886 685
Size: 3201 2318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 466 477 1441 1453 1582 1618 2101 2196
Size: 348 11 964 12 129 36 483 95
Cuts at: 2196 118
Size: 3441
Fragments arranged by size:
3441 964 483 348 129 95 36 12
11
Bpu10I CC'TnA_GC
Cuts at: 619 680 1825 2074 2437 619
Size: 61 1145 249 363 3701
Fragments arranged by size:
3701 1145 363 249 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 1741 2695 3574 4066 4364 1657
Size: 84 954 879 492 298 2812
Fragments arranged by size:
2812 954 879 492 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 5223 5223
Size: 5519
BsaAI yAC'GTr
Cuts at: 2106 2872 2106
Size: 766 4753
BsaBI GATnn'nnATC
Cuts at: 6 3033 5070 6
Size: 3027 2037 455
Fragments arranged by size:
3027 2037 455
BsaHI Gr'CG_yC
Cuts at: 957 1202 2950 3198 3900 5396 957
Size: 245 1748 248 702 1496 1080
Fragments arranged by size:
1748 1496 1080 702 248 245
BsaWI w'CCGG_w
Cuts at: 784 2708 3229 3907 4181 4328 4739 784
Size: 1924 521 678 274 147 411 1564
Fragments arranged by size:
1924 1564 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 187 217 496 526 1234 1264 1889 1919 187
Size: 30 279 30 708 30 625 30 3787
Fragments arranged by size:
3787 708 625 279 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 643 1060 1063 1096 1615 2104 2113 289
Size: 354 417 3 33 519 489 9 3695
Fragments arranged by size:
3695 519 489 417 354 33 9 3
BseYI C'CCAG_C
Cuts at: 394 431 438 1117 2944 3702 4279 4775 394
Size: 37 7 679 1827 758 577 496 1138
Fragments arranged by size:
1827 1138 758 679 577 496 37 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1789 2278 706
Size: 1083 489 3947
Fragments arranged by size:
3947 1083 489
BsiEI CG_ry'CG
Cuts at: 104 3061 3107 4315 104
Size: 2957 46 1208 1308
Fragments arranged by size:
2957 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 625 946 1396 2533 3311 3501 4293 625
Size: 321 450 1137 778 190 792 1851
Fragments arranged by size:
1851 1137 792 778 450 321 190
BsiWI C'GTAC_G
Cuts at: 1188 1188
Size: 5519
Bsp1286I G_dGCh'C
Cuts at: 241 288 432 625 635 765 902 946 1057
Size: 47 144 193 10 130 137 44 111
Cuts at: 1057 1396 1717 2166 2209 2408 2533 3144 3237
Size: 339 321 449 43 199 125 611 93
Cuts at: 3237 3311 3501 3563 4293 241
Size: 74 190 62 730 1467
Fragments arranged by size:
1467 730 611 449 339 321 199 193
190 144 137 130 125 111 93 74
62 47 44 43 10
BspEI T'CCGG_A
Cuts at: 4739 4739
Size: 5519
BspHI T'CATG_A
Cuts at: 4695 4695
Size: 5519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 1514 2052 2569 2783 3085 3466 132
Size: 1382 538 517 214 302 381 2185
Fragments arranged by size:
2185 1382 538 517 381 302 214
BsrBI CCG'CTC
Cuts at: 50 684 842 954 3811 3865 5349 5514 50
Size: 634 158 112 2857 54 1484 165 55
Fragments arranged by size:
2857 1484 634 165 158 112 55 54
BsrDI GCAATG_nn'
Cuts at: 1785 3431 1785
Size: 1646 3873
BsrFI r'CCGG_y
Cuts at: 3517 3698 3907 3517
Size: 181 209 5129
Fragments arranged by size:
5129 209 181
BsrGI T'GTAC_A
Cuts at: 1788 1788
Size: 5519
BssSI C'ACGA_G
Cuts at: 878 3790 4148 878
Size: 2912 358 2249
Fragments arranged by size:
2912 2249 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 2896 1936
Size: 960 4559
BstBI TT'CG_AA
Cuts at: 3880 3880
Size: 5519
BstXI CCAn_nnnn'nTGG
Cuts at: 148 2496 148
Size: 2348 3171
BstYI r'GATC_y
Cuts at: 1 1835 2192 2544 3011 3034 3369 3615 4627
Size: 1834 357 352 467 23 335 246 1012
Cuts at: 4627 4713 4725 5071 1
Size: 86 12 346 449
Fragments arranged by size:
1834 1012 467 449 357 352 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 495 1099 2127 107
Size: 388 604 1028 3499
Fragments arranged by size:
3499 1028 604 388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2202 3469 3650 3813 2202
Size: 1267 181 163 3908
Fragments arranged by size:
3908 1267 181 163
BtsI GCAGTG_nn'
Cuts at: 161 188 1366 1479 1918 2290 2762 161
Size: 27 1178 113 439 372 472 2918
Fragments arranged by size:
2918 1178 472 439 372 113 27
DraI TTT'AAA
Cuts at: 2518 2518
Size: 5519
DrdI GACnn_nn'nnGTC
Cuts at: 458 2233 3225 4083 458
Size: 1775 992 858 1894
Fragments arranged by size:
1894 1775 992 858
EaeI y'GGCC_r
Cuts at: 340 412 594 859 1010 1208 1733 2100 3104
Size: 72 182 265 151 198 525 367 1004
Cuts at: 3104 3278 3669 3696 5041 340
Size: 174 391 27 1345 818
Fragments arranged by size:
1345 1004 818 525 391 367 265 198
182 174 151 72 27
EagI C'GGCC_G
Cuts at: 3104 3104
Size: 5519
EarI CTCTTCn'nnn_
Cuts at: 542 732 914 1795 1984 2417 3542 3752 542
Size: 190 182 881 189 433 1125 210 2309
Fragments arranged by size:
2309 1125 881 433 210 190 189 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 759 2022 2365 3727 4037 4183 5329 759
Size: 1263 343 1362 310 146 1146 949
Fragments arranged by size:
1362 1263 1146 949 343 310 146
EcoICRI GAG'CTC
Cuts at: 2531 2531
Size: 5519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 247 466 477 1437 1441 1453 1582 1618
Size: 129 219 11 960 4 12 129 36
Cuts at: 1618 1717 1739 1966 2101 2196 3344 3776 4523
Size: 99 22 227 135 95 1148 432 747
Cuts at: 4523 4791 118
Size: 268 846
Fragments arranged by size:
1148 960 846 747 432 268 227 219
135 129 129 99 95 36 22 12
11 4
EcoNI CCTnn'n_nnAGG
Cuts at: 326 991 1656 326
Size: 665 665 4189
Fragments arranged by size:
4189 665 665
EcoO109I rG'GnC_Cy
Cuts at: 311 451 552 615 676 1053 1416 2070 2342
Size: 140 101 63 61 377 363 654 272
Cuts at: 2342 2658 311
Size: 316 3172
Fragments arranged by size:
3172 654 377 363 316 272 140 101
63 61
EcoRI G'AATT_C
Cuts at: 747 2523 747
Size: 1776 3743
EcoRV GAT'ATC
Cuts at: 1179 1179
Size: 5519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1857 3538 1857
Size: 1681 3838
FspI TGC'GCA
Cuts at: 3300 3300
Size: 5519
HaeII r_GCGC'y
Cuts at: 486 693 960 1019 1143 1554 2444 2953 3201
Size: 207 267 59 124 411 890 509 248
Cuts at: 3201 4223 5202 486
Size: 1022 979 803
Fragments arranged by size:
1022 979 890 803 509 411 267 248
207 124 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 496 528 838 870 1323 1355 2317
Size: 32 277 32 310 32 453 32 962
Cuts at: 2317 2349 3361 3391 3393 3423 187
Size: 32 1012 30 2 30 2283
Fragments arranged by size:
2283 1012 962 453 310 277 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2558 2558
Size: 5519
KasI G'GCGC_C
Cuts at: 956 2949 3197 956
Size: 1993 248 3278
Fragments arranged by size:
3278 1993 248
KpnI G_GTAC'C
Cuts at: 2539 2539
Size: 5519
MluI A'CGCG_T
Cuts at: 4952 4952
Size: 5519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4190 4374 4190
Size: 184 5335
MscI TGG'CCA
Cuts at: 342 414 596 1012 1210 1735 3280 342
Size: 72 182 416 198 525 1545 2581
Fragments arranged by size:
2581 1545 525 416 198 182 72
MslI CAynn'nnrTG
Cuts at: 661 2300 3508 3635 661
Size: 1639 1208 127 2545
Fragments arranged by size:
2545 1639 1208 127
MspA1I CmG'CkG
Cuts at: 299 380 410 1101 1199 1428 1863 1880 2630
Size: 81 30 691 98 229 435 17 750
Cuts at: 2630 2944 3304 4317 4562 299
Size: 314 360 1013 245 1256
Fragments arranged by size:
1256 1013 750 691 435 360 314 245
229 98 81 30 17
NaeI GCC'GGC
Cuts at: 3700 3700
Size: 5519
NarI GG'CG_CC
Cuts at: 957 2950 3198 957
Size: 1993 248 3278
Fragments arranged by size:
3278 1993 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5519
NgoMIV G'CCGG_C
Cuts at: 3698 3698
Size: 5519
NspI r_CATG'y
Cuts at: 2572 3072 3979 2572
Size: 500 907 4112
Fragments arranged by size:
4112 907 500
PasI CC'CwG_GG
Cuts at: 306 635 306
Size: 329 5190
PciI A'CATG_T
Cuts at: 3975 3975
Size: 5519
PflMI CCAn_nnn'nTGG
Cuts at: 868 868
Size: 5519
PfoI T'CCnGG_A
Cuts at: 937 1559 2459 937
Size: 622 900 3997
Fragments arranged by size:
3997 900 622
PmeI GTTT'AAAC
Cuts at: 2518 2518
Size: 5519
PmlI CAC'GTG
Cuts at: 2106 2106
Size: 5519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 4658
Size: 32 5487
PpuMI rG'GwC_Cy
Cuts at: 311 451 552 676 1416 2070 2342 311
Size: 140 101 124 740 654 272 3488
Fragments arranged by size:
3488 740 654 272 140 124 101
PspOMI G'GGCC_C
Cuts at: 1053 2162 1053
Size: 1109 4410
PstI C_TGCA'G
Cuts at: 1241 1415 1929 2063 2090 2566 3251 1241
Size: 174 514 134 27 476 685 3509
Fragments arranged by size:
3509 685 514 476 174 134 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5519
PvuII CAG'CTG
Cuts at: 410 2944 3304 410
Size: 2534 360 2625
Fragments arranged by size:
2625 2534 360
RsrII CG'GwC_CG
Cuts at: 3714 3714
Size: 5519
SacI G_AGCT'C
Cuts at: 2533 2533
Size: 5519
SacII CC_GC'GG
Cuts at: 1102 1102
Size: 5519
SalI G'TCGA_C
Cuts at: 2556 2556
Size: 5519
SanDI GG'GwC_CC
Cuts at: 311 451 311
Size: 140 5379
SapI GCTCTTCn'nnn_
Cuts at: 914 3542 3752 914
Size: 2628 210 2681
Fragments arranged by size:
2681 2628 210
SbfI CC_TGCA'GG
Cuts at: 1415 2566 1415
Size: 1151 4368
ScaI AGT'ACT
Cuts at: 5036 5036
Size: 5519
SfcI C'TryA_G
Cuts at: 33 176 1237 1411 1925 2059 2086 2562 3247
Size: 143 1061 174 514 134 27 476 685
Cuts at: 3247 4240 4431 33
Size: 993 191 1121
Fragments arranged by size:
1121 1061 993 685 514 476 191 174
143 134 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 1741 1741
Size: 5519
SfoI GGC'GCC
Cuts at: 958 2951 3199 958
Size: 1993 248 3278
Fragments arranged by size:
3278 1993 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
SmaI CCC'GGG
Cuts at: 760 796 1058 2541 760
Size: 36 262 1483 3738
Fragments arranged by size:
3738 1483 262 36
SmlI C'TyrA_G
Cuts at: 1672 1756 2674 3589 4081 4343 1672
Size: 84 918 915 492 262 2848
Fragments arranged by size:
2848 918 915 492 262 84
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5519
SphI G_CATG'C
Cuts at: 2572 3072 2572
Size: 500 5019
SrfI GCCC'GGGC
Cuts at: 760 760
Size: 5519
StuI AGG'CCT
Cuts at: 1273 1528 1744 2413 1273
Size: 255 216 669 4379
Fragments arranged by size:
4379 669 255 216
StyI C'CwwG_G
Cuts at: 107 327 1739 2312 2653 3007 107
Size: 220 1412 573 341 354 2619
Fragments arranged by size:
2619 1412 573 354 341 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2312 2391 3102 3235 3694 3903 2312
Size: 79 711 133 459 209 3928
Fragments arranged by size:
3928 711 459 209 133 79
TatI w'GTAC_w
Cuts at: 1788 5034 1788
Size: 3246 2273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1345 1377 2160 2192 5036 5068 1345
Size: 32 783 32 2844 32 1796
Fragments arranged by size:
2844 1796 783 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3316 3316
Size: 5519
XbaI T'CTAG_A
Cuts at: 2550 2550
Size: 5519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2493 3014 2493
Size: 521 4998
XmaI C'CCGG_G
Cuts at: 758 794 1056 2539 758
Size: 36 262 1483 3738
Fragments arranged by size:
3738 1483 262 36
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
PspXI PsrI SexAI SgrAI SnaBI SspI SwaI XhoI
XmnI ZraI