Restriction map for
pF1KE4045
(Circular) (Six-base) MAPSORT of: pF1KE4045.seq Check: 6372 from: 1 to: 3914
pF1KE4045 3914 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 952 952
Size: 3914
Acc65I G'GTAC_C
Cuts at: 930 930
Size: 3914
AclI AA'CG_TT
Cuts at: 3754 3754
Size: 3914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1739 2171 2918 3186 703
Size: 1036 432 747 268 1431
Fragments arranged by size:
1431 1036 747 432 268
AflII C'TTAA_G
Cuts at: 469 469
Size: 3914
AflIII A'CryG_T
Cuts at: 1266 2370 3347 1266
Size: 1104 977 1833
Fragments arranged by size:
1833 1104 977
AgeI A'CCGG_T
Cuts at: 2302 2302
Size: 3914
AlwNI CAG_nnn'CTG
Cuts at: 123 150 379 1219 2786 123
Size: 27 229 840 1567 1251
Fragments arranged by size:
1567 1251 840 229 27
ApaLI G'TGCA_C
Cuts at: 2684 2684
Size: 3914
ApoI r'AATT_y
Cuts at: 918 3267 3523 918
Size: 2349 256 1309
Fragments arranged by size:
2349 1309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3914
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
AvaI C'yCGr_G
Cuts at: 934 934
Size: 3914
BamHI G'GATC_C
Cuts at: 939 939
Size: 3914
BanI G'GyrC_C
Cuts at: 930 1344 1592 1627 930
Size: 414 248 35 3217
Fragments arranged by size:
3217 414 248 35
BanII G_rGCy'C
Cuts at: 500 928 1958 500
Size: 428 1030 2456
Fragments arranged by size:
2456 1030 428
BbeI G_GCGC'C
Cuts at: 1348 1596 1348
Size: 248 3666
BbsI GAAGACnn'nnnn_
Cuts at: 583 740 905 583
Size: 157 165 3592
Fragments arranged by size:
3592 165 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 181 215 181
Size: 34 3880
BciVI GTATCCnnnnn_n'
Cuts at: 1812 2573 3914 1812
Size: 761 1341 1812
Fragments arranged by size:
1812 1341 761
BclI T'GATC_A
Cuts at: 1434 1434
Size: 3914
BglII A'GATC_T
Cuts at: 1429 3466 1429
Size: 2037 1877
BlpI GC'TnA_GC
Cuts at: 1026 1026
Size: 3914
Bme1580I G_kGCm'C
Cuts at: 1539 1632 2688 1539
Size: 93 1056 2765
Fragments arranged by size:
2765 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1126 1207 1542 3686 1126
Size: 81 335 2144 1354
Fragments arranged by size:
2144 1354 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2281 2281
Size: 3914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1969 2461 2759 1090
Size: 879 492 298 2245
Fragments arranged by size:
2245 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 828 3618 828
Size: 2790 1124
BsaAI yAC'GTr
Cuts at: 1267 1267
Size: 3914
BsaBI GATnn'nnATC
Cuts at: 6 1428 3465 6
Size: 1422 2037 455
Fragments arranged by size:
2037 1422 455
BsaHI Gr'CG_yC
Cuts at: 1345 1593 2295 3791 1345
Size: 248 702 1496 1468
Fragments arranged by size:
1496 1468 702 248
BsaWI w'CCGG_w
Cuts at: 1103 1624 2302 2576 2723 3134 1103
Size: 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 507 1339 2097 2674 3170 507
Size: 832 758 577 496 1251
Fragments arranged by size:
1251 832 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 3914
BsiEI CG_ry'CG
Cuts at: 104 201 1456 1502 2710 104
Size: 97 1255 46 1208 1308
Fragments arranged by size:
1308 1255 1208 97 46
BsiHKAI G_wGCw'C
Cuts at: 928 1706 1896 2688 928
Size: 778 190 792 2154
Fragments arranged by size:
2154 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 500 928 1539 1632 1706 1896 1958 2688 500
Size: 428 611 93 74 190 62 730 1726
Fragments arranged by size:
1726 730 611 428 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3134 3134
Size: 3914
BspHI T'CATG_A
Cuts at: 3090 3090
Size: 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 964 1178 1480 1861 964
Size: 214 302 381 3017
Fragments arranged by size:
3017 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2206 2260 3744 3909 50
Size: 2156 54 1484 165 55
Fragments arranged by size:
2156 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1826 1826
Size: 3914
BsrFI r'CCGG_y
Cuts at: 1912 2093 2302 1912
Size: 181 209 3524
Fragments arranged by size:
3524 209 181
BssSI C'ACGA_G
Cuts at: 2185 2543 2185
Size: 358 3556
BstAPI GCAn_nnn'nTGC
Cuts at: 150 1291 150
Size: 1141 2773
BstBI TT'CG_AA
Cuts at: 2275 2275
Size: 3914
BstYI r'GATC_y
Cuts at: 1 376 658 939 1406 1429 1764 2010 3022
Size: 375 282 281 467 23 335 246 1012
Cuts at: 3022 3108 3120 3466 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 375 346 335 282 281
246 86 23 12
BtgI C'CryG_G
Cuts at: 186 224 186
Size: 38 3876
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1864 2045 2208 1864
Size: 181 163 3570
Fragments arranged by size:
3570 181 163
BtsI GCAGTG_nn'
Cuts at: 199 265 1157 199
Size: 66 892 2956
Fragments arranged by size:
2956 892 66
DraI TTT'AAA
Cuts at: 913 913
Size: 3914
DrdI GACnn_nn'nnGTC
Cuts at: 1620 2478 1620
Size: 858 3056
EaeI y'GGCC_r
Cuts at: 1499 1673 2064 2091 3436 1499
Size: 174 391 27 1345 1977
Fragments arranged by size:
1977 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1499 1499
Size: 3914
EarI CTCTTCn'nnn_
Cuts at: 1937 2147 1937
Size: 210 3704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2122 2432 2578 3724 2122
Size: 310 146 1146 2312
Fragments arranged by size:
2312 1146 310 146
EcoICRI GAG'CTC
Cuts at: 926 926
Size: 3914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1739 2171 2918 3186 703
Size: 1036 432 747 268 1431
Fragments arranged by size:
1431 1036 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1053 1053
Size: 3914
EcoRI G'AATT_C
Cuts at: 918 918
Size: 3914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3914
FspI TGC'GCA
Cuts at: 1695 1695
Size: 3914
HaeII r_GCGC'y
Cuts at: 1348 1596 2618 3597 1348
Size: 248 1022 979 1665
Fragments arranged by size:
1665 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1756 1786 1788 1818 1756
Size: 30 2 30 3852
Fragments arranged by size:
3852 30 30 2
HincII GTy'rAC
Cuts at: 953 953
Size: 3914
HindIII A'AGCT_T
Cuts at: 306 306
Size: 3914
KasI G'GCGC_C
Cuts at: 1344 1592 1344
Size: 248 3666
KpnI G_GTAC'C
Cuts at: 934 934
Size: 3914
MluI A'CGCG_T
Cuts at: 3347 3347
Size: 3914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2769 2585
Size: 184 3730
MscI TGG'CCA
Cuts at: 1675 1675
Size: 3914
MslI CAynn'nnrTG
Cuts at: 1903 2030 1903
Size: 127 3787
MspA1I CmG'CkG
Cuts at: 511 1025 1339 1699 2712 2957 511
Size: 514 314 360 1013 245 1468
Fragments arranged by size:
1468 1013 514 360 314 245
NaeI GCC'GGC
Cuts at: 2095 2095
Size: 3914
NarI GG'CG_CC
Cuts at: 1345 1593 1345
Size: 248 3666
NgoMIV G'CCGG_C
Cuts at: 2093 2093
Size: 3914
NspI r_CATG'y
Cuts at: 967 1467 2374 967
Size: 500 907 2507
Fragments arranged by size:
2507 907 500
PciI A'CATG_T
Cuts at: 2370 2370
Size: 3914
PflMI CCAn_nnn'nTGG
Cuts at: 292 292
Size: 3914
PfoI T'CCnGG_A
Cuts at: 787 787
Size: 3914
PmeI GTTT'AAAC
Cuts at: 913 913
Size: 3914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 3882
PstI C_TGCA'G
Cuts at: 961 1646 961
Size: 685 3229
PvuI CG_AT'CG
Cuts at: 104 201 104
Size: 97 3817
PvuII CAG'CTG
Cuts at: 511 1339 1699 511
Size: 828 360 2726
Fragments arranged by size:
2726 828 360
RsrII CG'GwC_CG
Cuts at: 2109 2109
Size: 3914
SacI G_AGCT'C
Cuts at: 928 928
Size: 3914
SalI G'TCGA_C
Cuts at: 951 951
Size: 3914
SapI GCTCTTCn'nnn_
Cuts at: 1937 2147 1937
Size: 210 3704
SbfI CC_TGCA'GG
Cuts at: 961 961
Size: 3914
ScaI AGT'ACT
Cuts at: 3431 3431
Size: 3914
SfcI C'TryA_G
Cuts at: 33 957 1642 2635 2826 33
Size: 924 685 993 191 1121
Fragments arranged by size:
1121 993 924 685 191
SfoI GGC'GCC
Cuts at: 1346 1594 1346
Size: 248 3666
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
SmaI CCC'GGG
Cuts at: 936 936
Size: 3914
SmlI C'TyrA_G
Cuts at: 469 1069 1984 2476 2738 469
Size: 600 915 492 262 1645
Fragments arranged by size:
1645 915 600 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3914
SphI G_CATG'C
Cuts at: 967 1467 967
Size: 500 3414
SspI AAT'ATT
Cuts at: 245 701 245
Size: 456 3458
StyI C'CwwG_G
Cuts at: 1048 1402 1048
Size: 354 3560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1497 1630 2089 2298 1497
Size: 133 459 209 3113
Fragments arranged by size:
3113 459 209 133
TatI w'GTAC_w
Cuts at: 3429 3429
Size: 3914
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 3882
Tth111I GACn'n_nGTC
Cuts at: 1711 1711
Size: 3914
XbaI T'CTAG_A
Cuts at: 945 945
Size: 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1409 1409
Size: 3914
XmaI C'CCGG_G
Cuts at: 934 934
Size: 3914
XmnI GAAnn'nnTTC
Cuts at: 344 344
Size: 3914
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI