Restriction map for
pF1KE3827
(Circular) (Six-base) MAPSORT of: pF1KE3827.seq Check: 5482 from: 1 to: 11564
pF1KE3827 11564 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 6683 8602 6683
Size: 1919 9645
Acc65I G'GTAC_C
Cuts at: 8580 8580
Size: 11564
AclI AA'CG_TT
Cuts at: 6393 11404 6393
Size: 5011 6553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 206 383 556 1127 1294 1429 1585 2659
Size: 15 177 173 571 167 135 156 1074
Cuts at: 2659 2855 2921 3025 3752 4187 4223 4258 4754
Size: 196 66 104 727 435 36 35 496
Cuts at: 4754 5653 6859 7396 9389 9821 10568 10836 191
Size: 899 1206 537 1993 432 747 268 919
Fragments arranged by size:
1993 1206 1074 919 899 747 727 571
537 496 435 432 268 196 177 173
167 156 135 104 66 36 35 15
AfeI AGC'GCT
Cuts at: 7719 7719
Size: 11564
AflII C'TTAA_G
Cuts at: 3079 3079
Size: 11564
AflIII A'CryG_T
Cuts at: 3938 5873 8916 10020 10997 3938
Size: 1935 3043 1104 977 4505
Fragments arranged by size:
4505 3043 1935 1104 977
AgeI A'CCGG_T
Cuts at: 9952 9952
Size: 11564
AhdI GACnn_n'nnGTC
Cuts at: 1160 1416 6948 1160
Size: 256 5532 5776
Fragments arranged by size:
5776 5532 256
AleI CACnn'nnGTG
Cuts at: 4735 4735
Size: 11564
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 341 3695 4901 341
Size: 3354 1206 7004
Fragments arranged by size:
7004 3354 1206
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 243 275 2535 2567 2955 2987 3310 3342 243
Size: 32 2260 32 388 32 323 32 8465
Fragments arranged by size:
8465 2260 388 323 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1540 1882 2101 3091 3156 7770 7869 8016 8869
Size: 342 219 990 65 4614 99 147 853
Cuts at: 8869 10436 1540
Size: 1567 2668
Fragments arranged by size:
4614 2668 1567 990 853 342 219 147
99 65
ApaI G_GGCC'C
Cuts at: 3732 3732
Size: 11564
ApaLI G'TGCA_C
Cuts at: 1067 1669 10334 1067
Size: 602 8665 2297
Fragments arranged by size:
8665 2297 602
ApoI r'AATT_y
Cuts at: 495 1860 2019 2598 5890 6533 7145 8568 10917
Size: 1365 159 579 3292 643 612 1423 2349
Cuts at: 10917 11173 495
Size: 256 886
Fragments arranged by size:
3292 2349 1423 1365 886 643 612 579
256 159
AseI AT'TA_AT
Cuts at: 20 5191 20
Size: 5171 6393
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11564
AvaI C'yCGr_G
Cuts at: 7193 7812 7903 8584 7193
Size: 619 91 681 10173
Fragments arranged by size:
10173 681 619 91
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3597 3630 3597
Size: 33 11531
BamHI G'GATC_C
Cuts at: 1023 4516 8207 8589 1023
Size: 3493 3691 382 3998
Fragments arranged by size:
3998 3691 3493 382
BanI G'GyrC_C
Cuts at: 530 2427 3159 4862 8580 8994 9242 9277 530
Size: 1897 732 1703 3718 414 248 35 2817
Fragments arranged by size:
3718 2817 1897 1703 732 414 248 35
BanII G_rGCy'C
Cuts at: 684 1079 1144 1565 3416 3692 3710 3732 3762
Size: 395 65 421 1851 276 18 22 30
Cuts at: 3762 4342 4768 5009 7194 7712 7819 8039 8240
Size: 580 426 241 2185 518 107 220 201
Cuts at: 8240 8578 9608 684
Size: 338 1030 2640
Fragments arranged by size:
2640 2185 1851 1030 580 518 426 421
395 338 276 241 220 201 107 65
30 22 18
BbeI G_GCGC'C
Cuts at: 8998 9246 8998
Size: 248 11316
BbsI GAAGACnn'nnnn_
Cuts at: 374 1751 2243 2636 5485 6409 374
Size: 1377 492 393 2849 924 5529
Fragments arranged by size:
5529 2849 1377 924 492 393
BbvCI CC'TCA_GC
Cuts at: 637 1088 3629 4661 7714 637
Size: 451 2541 1032 3053 4487
Fragments arranged by size:
4487 3053 2541 1032 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 757 791 2125 2159 757
Size: 34 1334 34 10162
Fragments arranged by size:
10162 1334 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 700 2271 6271 9462 10223 11564 700
Size: 1571 4000 3191 761 1341 700
Fragments arranged by size:
4000 3191 1571 1341 761 700
BclI T'GATC_A
Cuts at: 1293 5103 6254 7019 9084 1293
Size: 3810 1151 765 2065 3773
Fragments arranged by size:
3810 3773 2065 1151 765
BfrBI ATG'CAT
Cuts at: 612 612
Size: 11564
BglI GCCn_nnn'nGGC
Cuts at: 2045 2812 3208 7975 2045
Size: 767 396 4767 5634
Fragments arranged by size:
5634 4767 767 396
BglII A'GATC_T
Cuts at: 5122 6636 7593 8549 9079 11116 5122
Size: 1514 957 956 530 2037 5570
Fragments arranged by size:
5570 2037 1514 957 956 530
BlpI GC'TnA_GC
Cuts at: 3410 7777 8143 8676 3410
Size: 4367 366 533 6298
Fragments arranged by size:
6298 4367 533 366
Bme1580I G_kGCm'C
Cuts at: 1038 1071 1342 1673 1719 3732 4867 5495 8056
Size: 33 271 331 46 2013 1135 628 2561
Cuts at: 8056 9189 9282 10338 1038
Size: 1133 93 1056 2264
Fragments arranged by size:
2561 2264 2013 1135 1133 1056 628 331
271 93 46 33
BmgBI CAC'GTC
Cuts at: 647 1668 1674 4158 4704 647
Size: 1021 6 2484 546 7507
Fragments arranged by size:
7507 2484 1021 546 6
BmrI ACTGGGnnnn_n'
Cuts at: 1473 1510 4381 4442 6010 6574 7890 8219 8776
Size: 37 2871 61 1568 564 1316 329 557
Cuts at: 8776 8857 9192 11336 1473
Size: 81 335 2144 1701
Fragments arranged by size:
2871 2144 1701 1568 1316 564 557 335
329 81 61 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4404 7326 8259 9931 4404
Size: 2922 933 1672 6037
Fragments arranged by size:
6037 2922 1672 933
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 816 932 1511 1676 1832 2096 2723 3613
Size: 16 116 579 165 156 264 627 890
Cuts at: 3613 3926 5092 5873 6310 7870 8023 8092 800
Size: 313 1166 781 437 1560 153 69 4272
Fragments arranged by size:
4272 1560 1166 890 781 627 579 437
313 264 165 156 153 116 69 16
Bpu10I CC'TnA_GC
Cuts at: 637 1088 1423 2876 3629 4133 4661 7714 637
Size: 451 335 1453 753 504 528 3053 4487
Fragments arranged by size:
4487 3053 1453 753 528 504 451 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 2395 3119 6142 6672 8122 8740 9619 10111
Size: 1431 724 3023 530 1450 618 879 492
Cuts at: 10111 10409 964
Size: 298 2119
Fragments arranged by size:
3023 2119 1450 1431 879 724 618 530
492 298
BsaI GGTCTCn'nnnn_
Cuts at: 268 3813 7510 11268 268
Size: 3545 3697 3758 564
Fragments arranged by size:
3758 3697 3545 564
BsaAI yAC'GTr
Cuts at: 6820 8917 6820
Size: 2097 9467
BsaBI GATnn'nnATC
Cuts at: 6 890 6259 7096 8206 9078 11115 6
Size: 884 5369 837 1110 872 2037 455
Fragments arranged by size:
5369 2037 1110 884 872 837 455
BsaHI Gr'CG_yC
Cuts at: 712 8995 9243 9945 11441 712
Size: 8283 248 702 1496 835
Fragments arranged by size:
8283 1496 835 702 248
BsaWI w'CCGG_w
Cuts at: 1071 4513 4790 4847 8753 9274 9952 10226 10373
Size: 3442 277 57 3906 521 678 274 147
Cuts at: 10373 10784 1071
Size: 411 1851
Fragments arranged by size:
3906 3442 1851 678 521 411 277 274
147 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 267 297 1424 1454 2535 2565 2955 2985 3208
Size: 30 1127 30 1081 30 390 30 223
Cuts at: 3208 3238 4937 4967 267
Size: 30 1699 30 6864
Fragments arranged by size:
6864 1699 1127 1081 390 223 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 784 1204 1207 1846 3287 3868 4111 4723 4726
Size: 420 3 639 1441 581 243 612 3
Cuts at: 4726 4729 4958 7015 7702 7921 8002 8005 8047
Size: 3 229 2057 687 219 81 3 42
Cuts at: 8047 8248 784
Size: 201 4100
Fragments arranged by size:
4100 2057 1441 687 639 612 581 420
243 229 219 201 81 42 3 3
3 3
BseYI C'CCAG_C
Cuts at: 627 2200 3732 4802 5804 7979 8989 9747 10324
Size: 1573 1532 1070 1002 2175 1010 758 577
Cuts at: 10324 10820 627
Size: 496 1371
Fragments arranged by size:
2175 1573 1532 1371 1070 1010 1002 758
577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 736 2114 4897 7305 8017 8237 337
Size: 399 1378 2783 2408 712 220 3664
Fragments arranged by size:
3664 2783 2408 1378 712 399 220
BsiEI CG_ry'CG
Cuts at: 104 9106 9152 10360 104
Size: 9002 46 1208 1308
Fragments arranged by size:
9002 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 684 1071 1123 1673 3218 3692 3710 3787 3912
Size: 387 52 550 1545 474 18 77 125
Cuts at: 3912 4768 5009 7712 7819 8039 8578 9356 9546
Size: 856 241 2703 107 220 539 778 190
Cuts at: 9546 10338 684
Size: 792 1910
Fragments arranged by size:
2703 1910 1545 856 792 778 550 539
474 387 241 220 190 125 107 77
52 18
BsmI GAATG_Cn'
Cuts at: 523 780 1406 3492 6390 6849 7554 523
Size: 257 626 2086 2898 459 705 4533
Fragments arranged by size:
4533 2898 2086 705 626 459 257
BsmBI CGTCTCn'nnnn_
Cuts at: 908 2072 6724 908
Size: 1164 4652 5748
Fragments arranged by size:
5748 4652 1164
Bsp1286I G_dGCh'C
Cuts at: 684 1038 1071 1079 1123 1144 1342 1565 1673
Size: 354 33 8 44 21 198 223 108
Cuts at: 1673 1719 3218 3416 3692 3710 3732 3762 3787
Size: 46 1499 198 276 18 22 30 25
Cuts at: 3787 3912 4342 4768 4867 5009 5495 7194 7712
Size: 125 430 426 99 142 486 1699 518
Cuts at: 7712 7819 8039 8056 8240 8578 9189 9282 9356
Size: 107 220 17 184 338 611 93 74
Cuts at: 9356 9546 9608 10338 684
Size: 190 62 730 1910
Fragments arranged by size:
1910 1699 1499 730 611 518 486 430
426 354 338 276 223 220 198 198
190 184 142 125 108 107 99 93
74 62 46 44 33 30 25 22
21 18 17 8
BspEI T'CCGG_A
Cuts at: 4790 10784 4790
Size: 5994 5570
BspHI T'CATG_A
Cuts at: 291 894 1565 2997 6164 6758 10740 291
Size: 603 671 1432 3167 594 3982 1115
Fragments arranged by size:
3982 3167 1432 1115 671 603 594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1758 7668 7886 8614 8828 9130 9511 1758
Size: 5910 218 728 214 302 381 3811
Fragments arranged by size:
5910 3811 728 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 3229 6294 7343 7962 8028 8139 8498 9856
Size: 3179 3065 1049 619 66 111 359 1358
Cuts at: 9856 9910 11394 11559 50
Size: 54 1484 165 55
Fragments arranged by size:
3179 3065 1484 1358 1049 619 359 165
111 66 55 54
BsrDI GCAATG_nn'
Cuts at: 278 1955 5868 9476 278
Size: 1677 3913 3608 2366
Fragments arranged by size:
3913 3608 2366 1677
BsrFI r'CCGG_y
Cuts at: 9562 9743 9952 9562
Size: 181 209 11174
Fragments arranged by size:
11174 209 181
BsrGI T'GTAC_A
Cuts at: 238 4288 5149 6176 238
Size: 4050 861 1027 5626
Fragments arranged by size:
5626 4050 1027 861
BssSI C'ACGA_G
Cuts at: 7940 9835 10193 7940
Size: 1895 358 9311
Fragments arranged by size:
9311 1895 358
BstAPI GCAn_nnn'nTGC
Cuts at: 424 1127 2101 3070 8941 424
Size: 703 974 969 5871 3047
Fragments arranged by size:
5871 3047 974 969 703
BstBI TT'CG_AA
Cuts at: 2602 9925 2602
Size: 7323 4241
BstEII G'GTnAC_C
Cuts at: 2901 2901
Size: 11564
BstXI CCAn_nnnn'nTGG
Cuts at: 794 1734 3702 3724 6030 794
Size: 940 1968 22 2306 6328
Fragments arranged by size:
6328 2306 1968 940 22
BstYI r'GATC_y
Cuts at: 1 1023 2211 4516 5067 5122 6198 6636 7038
Size: 1022 1188 2305 551 55 1076 438 402
Cuts at: 7038 7406 7593 8207 8549 8589 9056 9079 9414
Size: 368 187 614 342 40 467 23 335
Cuts at: 9414 9660 10672 10758 10770 11116 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2305 1188 1076 1022 1012 614 551 467
449 438 402 368 346 342 335 246
187 86 55 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 3833 4052 4759 8231 3833
Size: 219 707 3472 7166
Fragments arranged by size:
7166 3472 707 219
BtgI C'CryG_G
Cuts at: 545 675 1079 3617 4061 4647 5696 545
Size: 130 404 2538 444 586 1049 6413
Fragments arranged by size:
6413 2538 1049 586 444 404 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4844 9514 9695 9858 4844
Size: 4670 181 163 6550
Fragments arranged by size:
6550 4670 181 163
BtsI GCAGTG_nn'
Cuts at: 1434 1538 2490 2660 3124 3910 4578 5595 5689
Size: 104 952 170 464 786 668 1017 94
Cuts at: 5689 6610 8807 1434
Size: 921 2197 4191
Fragments arranged by size:
4191 2197 1017 952 921 786 668 464
170 104 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 6402 6437 6402
Size: 35 11529
DraI TTT'AAA
Cuts at: 939 4598 6398 6968 8563 939
Size: 3659 1800 570 1595 3940
Fragments arranged by size:
3940 3659 1800 1595 570
DraIII CAC_nnn'GTG
Cuts at: 551 1127 3061 4450 551
Size: 576 1934 1389 7665
Fragments arranged by size:
7665 1934 1389 576
DrdI GACnn_nn'nnGTC
Cuts at: 1672 9270 10128 1672
Size: 7598 858 3108
Fragments arranged by size:
7598 3108 858
EaeI y'GGCC_r
Cuts at: 672 1050 2620 3179 7587 7871 9149 9323 9714
Size: 378 1570 559 4408 284 1278 174 391
Cuts at: 9714 9741 11086 672
Size: 27 1345 1150
Fragments arranged by size:
4408 1570 1345 1278 1150 559 391 378
284 174 27
EagI C'GGCC_G
Cuts at: 9149 9149
Size: 11564
EarI CTCTTCn'nnn_
Cuts at: 167 1103 1244 2787 2940 2948 3414 4325 4484
Size: 936 141 1543 153 8 466 911 159
Cuts at: 4484 4997 5057 5279 5332 5395 6763 7244 7342
Size: 513 60 222 53 63 1368 481 98
Cuts at: 7342 7349 7568 8540 9587 9797 167
Size: 7 219 972 1047 210 1934
Fragments arranged by size:
1934 1543 1368 1047 972 936 911 513
481 466 222 219 210 159 153 141
98 63 60 53 8 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6211 9772 10082 10228 11374 6211
Size: 3561 310 146 1146 6401
Fragments arranged by size:
6401 3561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 682 3690 3708 4766 5007 7710 7817 8037 8576
Size: 3008 18 1058 241 2703 107 220 539
Cuts at: 8576 682
Size: 3670
Fragments arranged by size:
3670 3008 2703 1058 539 241 220 107
18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 191 206 383 556 800 816 932 1127 1294
Size: 15 177 173 244 16 116 195 167
Cuts at: 1294 1429 1511 1585 1676 1832 2096 2659 2723
Size: 135 82 74 91 156 264 563 64
Cuts at: 2723 2855 2921 3025 3613 3752 3926 4187 4223
Size: 132 66 104 588 139 174 261 36
Cuts at: 4223 4258 4754 5092 5653 5873 6310 6859 7396
Size: 35 496 338 561 220 437 549 537
Cuts at: 7396 7870 8023 8092 9389 9821 10568 10836 191
Size: 474 153 69 1297 432 747 268 919
Fragments arranged by size:
1297 919 747 588 563 561 549 537
496 474 437 432 338 268 264 261
244 220 195 177 174 173 167 156
153 139 135 132 116 104 91 82
74 69 66 64 36 35 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4791 6661 7627 8020 8173 4791
Size: 1870 966 393 153 8182
Fragments arranged by size:
8182 1870 966 393 153
EcoO109I rG'GnC_Cy
Cuts at: 339 475 2728 3728 3729 3964 4129 4807 5521
Size: 136 2253 1000 1 235 165 678 714
Cuts at: 5521 7372 7866 7968 8013 8166 8344 8406 8703
Size: 1851 494 102 45 153 178 62 297
Cuts at: 8703 339
Size: 3200
Fragments arranged by size:
3200 2253 1851 1000 714 678 494 297
235 178 165 153 136 102 62 45
1
EcoRI G'AATT_C
Cuts at: 495 1860 6533 7145 8568 495
Size: 1365 4673 612 1423 3491
Fragments arranged by size:
4673 3491 1423 1365 612
EcoRV GAT'ATC
Cuts at: 4509 4509
Size: 11564
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 358 2863 4420 5683 6895 6945 7228 9583 358
Size: 2505 1557 1263 1212 50 283 2355 2339
Fragments arranged by size:
2505 2355 2339 1557 1263 1212 283 50
FspI TGC'GCA
Cuts at: 9345 9345
Size: 11564
HaeII r_GCGC'y
Cuts at: 7721 7792 7863 8998 9246 10268 11247 7721
Size: 71 71 1135 248 1022 979 8038
Fragments arranged by size:
8038 1135 1022 979 248 71 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 831 863 978 1010 1146 1178 1402
Size: 32 532 32 115 32 136 32 224
Cuts at: 1402 1434 1442 1474 1936 1968 3415 3447 3552
Size: 32 8 32 462 32 1447 32 105
Cuts at: 3552 3584 4300 4332 4937 4969 5059 5088 5091
Size: 32 716 32 605 32 90 29 3
Cuts at: 5091 5120 5671 5703 5860 5892 6004 6036 6183
Size: 29 551 32 157 32 112 32 147
Cuts at: 6183 6215 6736 6768 6934 6966 7023 7055 7416
Size: 32 521 32 166 32 57 32 361
Cuts at: 7416 7448 9406 9436 9438 9468 267
Size: 32 1958 30 2 30 2363
Fragments arranged by size:
2363 1958 1447 716 605 551 532 521
462 361 224 166 157 147 136 115
112 105 90 57 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
30 29 29 8 3 2
HincII GTy'rAC
Cuts at: 644 2110 5040 5487 5945 6953 8447 8603 644
Size: 1466 2930 447 458 1008 1494 156 3605
Fragments arranged by size:
3605 2930 1494 1466 1008 458 447 156
HindIII A'AGCT_T
Cuts at: 6811 6811
Size: 11564
HpaI GTT'AAC
Cuts at: 8447 8447
Size: 11564
KasI G'GCGC_C
Cuts at: 8994 9242 8994
Size: 248 11316
KpnI G_GTAC'C
Cuts at: 8584 8584
Size: 11564
MluI A'CGCG_T
Cuts at: 10997 10997
Size: 11564
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 836 978 3288 3528 4347 4935 6031 7160 7226
Size: 142 2310 240 819 588 1096 1129 66
Cuts at: 7226 7370 10235 10419 836
Size: 144 2865 184 1981
Fragments arranged by size:
2865 2310 1981 1129 1096 819 588 240
184 144 142 66
MscI TGG'CCA
Cuts at: 1052 3181 7589 7873 9325 1052
Size: 2129 4408 284 1452 3291
Fragments arranged by size:
4408 3291 2129 1452 284
MslI CAynn'nnrTG
Cuts at: 3700 3722 4571 4735 6763 7030 9553 9680 3700
Size: 22 849 164 2028 267 2523 127 5584
Fragments arranged by size:
5584 2523 2028 849 267 164 127 22
MspA1I CmG'CkG
Cuts at: 677 1304 1654 1879 3016 3611 3736 3904 4802
Size: 627 350 225 1137 595 125 168 898
Cuts at: 4802 5531 6030 6564 7088 8121 8151 8675 8989
Size: 729 499 534 524 1033 30 524 314
Cuts at: 8989 9349 10362 10607 677
Size: 360 1013 245 1634
Fragments arranged by size:
1634 1137 1033 1013 898 729 627 595
534 524 524 499 360 350 314 245
225 168 125 30
NaeI GCC'GGC
Cuts at: 9745 9745
Size: 11564
NarI GG'CG_CC
Cuts at: 8995 9243 8995
Size: 248 11316
NcoI C'CATG_G
Cuts at: 3617 4647 3617
Size: 1030 10534
NdeI CA'TA_TG
Cuts at: 6408 6408
Size: 11564
NgoMIV G'CCGG_C
Cuts at: 9743 9743
Size: 11564
NsiI A_TGCA'T
Cuts at: 614 614
Size: 11564
NspI r_CATG'y
Cuts at: 2108 3942 5877 8617 9117 10024 2108
Size: 1834 1935 2740 500 907 3648
Fragments arranged by size:
3648 2740 1935 1834 907 500
PasI CC'CwG_GG
Cuts at: 1135 3330 1135
Size: 2195 9369
PciI A'CATG_T
Cuts at: 3938 5873 10020 3938
Size: 1935 4147 5482
Fragments arranged by size:
5482 4147 1935
PflMI CCAn_nnn'nTGG
Cuts at: 551 910 1586 2974 3156 4653 5023 7770 7869
Size: 359 676 1388 182 1497 370 2747 99
Cuts at: 7869 551
Size: 4246
Fragments arranged by size:
4246 2747 1497 1388 676 370 359 182
99
PfoI T'CCnGG_A
Cuts at: 383 793 909 2973 4358 5208 7268 383
Size: 410 116 2064 1385 850 2060 4679
Fragments arranged by size:
4679 2064 2060 1385 850 410 116
PmeI GTTT'AAAC
Cuts at: 8563 8563
Size: 11564
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1547 1579 2535 2567 2764 2796 2955 2987 8305
Size: 32 956 32 197 32 159 32 5318
Cuts at: 8305 8337 10703 10735 1547
Size: 32 2366 32 2376
Fragments arranged by size:
5318 2376 2366 956 197 159 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 475 2728 3964 5521 7372 7866 8013 8166 475
Size: 2253 1236 1557 1851 494 147 153 3873
Fragments arranged by size:
3873 2253 1851 1557 1236 494 153 147
PshAI GACnn'nnGTC
Cuts at: 8171 8171
Size: 11564
PspOMI G'GGCC_C
Cuts at: 3728 3728
Size: 11564
PspXI vC'TCGA_Gb
Cuts at: 7903 7903
Size: 11564
PstI C_TGCA'G
Cuts at: 1545 2670 2874 3286 3674 7727 7742 7883 8611
Size: 1125 204 412 388 4053 15 141 728
Cuts at: 8611 9296 1545
Size: 685 3813
Fragments arranged by size:
4053 3813 1125 728 685 412 388 204
141 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11564
PvuII CAG'CTG
Cuts at: 1304 1654 1879 3016 3611 3736 4802 5531 6030
Size: 350 225 1137 595 125 1066 729 499
Cuts at: 6030 7088 8121 8989 9349 1304
Size: 1058 1033 868 360 3519
Fragments arranged by size:
3519 1137 1066 1058 1033 868 729 595
499 360 350 225 125
RsrII CG'GwC_CG
Cuts at: 5744 9759 5744
Size: 4015 7549
SacI G_AGCT'C
Cuts at: 684 3692 3710 4768 5009 7712 7819 8039 8578
Size: 3008 18 1058 241 2703 107 220 539
Cuts at: 8578 684
Size: 3670
Fragments arranged by size:
3670 3008 2703 1058 539 241 220 107
18
SacII CC_GC'GG
Cuts at: 678 678
Size: 11564
SalI G'TCGA_C
Cuts at: 8601 8601
Size: 11564
SanDI GG'GwC_CC
Cuts at: 2728 5521 2728
Size: 2793 8771
SapI GCTCTTCn'nnn_
Cuts at: 2948 4484 4997 5395 9587 9797 2948
Size: 1536 513 398 4192 210 4715
Fragments arranged by size:
4715 4192 1536 513 398 210
SbfI CC_TGCA'GG
Cuts at: 8611 8611
Size: 11564
ScaI AGT'ACT
Cuts at: 246 2457 4011 4047 4882 11081 246
Size: 2211 1554 36 835 6199 729
Fragments arranged by size:
6199 2211 1554 835 729 36
SexAI A'CCwGG_T
Cuts at: 7646 7646
Size: 11564
SfcI C'TryA_G
Cuts at: 33 1212 1541 2570 2666 2870 3282 3514 3670
Size: 1179 329 1029 96 204 412 232 156
Cuts at: 3670 4214 4641 5532 6680 7723 7738 7879 8607
Size: 544 427 891 1148 1043 15 141 728
Cuts at: 8607 9292 10285 10476 33
Size: 685 993 191 1121
Fragments arranged by size:
1179 1148 1121 1043 1029 993 891 728
685 544 427 412 329 232 204 191
156 141 96 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 7975 7975
Size: 11564
SfoI GGC'GCC
Cuts at: 8996 9244 8996
Size: 248 11316
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11564
SmaI CCC'GGG
Cuts at: 8586 8586
Size: 11564
SmlI C'TyrA_G
Cuts at: 979 2374 3079 3134 6157 6651 7903 8101 8719
Size: 1395 705 55 3023 494 1252 198 618
Cuts at: 8719 9634 10126 10388 979
Size: 915 492 262 2155
Fragments arranged by size:
3023 2155 1395 1252 915 705 618 494
492 262 198 55
SpeI A'CTAG_T
Cuts at: 67 3340 67
Size: 3273 8291
SphI G_CATG'C
Cuts at: 8617 9117 8617
Size: 500 11064
SspI AAT'ATT
Cuts at: 1644 5786 6599 1644
Size: 4142 813 6609
Fragments arranged by size:
6609 4142 813
StuI AGG'CCT
Cuts at: 1349 2645 3931 1349
Size: 1296 1286 8982
Fragments arranged by size:
8982 1296 1286
StyI C'CwwG_G
Cuts at: 728 2498 3026 3203 3617 3683 4647 5729 6467
Size: 1770 528 177 414 66 964 1082 738
Cuts at: 6467 6788 8698 9052 728
Size: 321 1910 354 3240
Fragments arranged by size:
3240 1910 1770 1082 964 738 528 414
354 321 177 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 917 2073 3457 3610 4461 4568 5030 5732 7477
Size: 1156 1384 153 851 107 462 702 1745
Cuts at: 7477 9147 9280 9739 9948 917
Size: 1670 133 459 209 2533
Fragments arranged by size:
2533 1745 1670 1384 1156 851 702 462
459 209 153 133 107
TatI w'GTAC_w
Cuts at: 238 244 1615 2455 4009 4045 4288 4401 4880
Size: 6 1371 840 1554 36 243 113 479
Cuts at: 4880 5149 6176 6923 11079 238
Size: 269 1027 747 4156 723
Fragments arranged by size:
4156 1554 1371 1027 840 747 723 479
269 243 113 36 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1424 1456 3208 3240 3647 3679 5216 5248 6756
Size: 32 1752 32 407 32 1537 32 1508
Cuts at: 6756 6788 11081 11113 1424
Size: 32 4293 32 1875
Fragments arranged by size:
4293 1875 1752 1537 1508 407 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1456 9361 1456
Size: 7905 3659
XbaI T'CTAG_A
Cuts at: 4926 4977 8595 4926
Size: 51 3618 7895
Fragments arranged by size:
7895 3618 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1486 3870 3957 4455 5514 9059 1486
Size: 2384 87 498 1059 3545 3991
Fragments arranged by size:
3991 3545 2384 1059 498 87
XhoI C'TCGA_G
Cuts at: 7903 7903
Size: 11564
XmaI C'CCGG_G
Cuts at: 8584 8584
Size: 11564
XmnI GAAnn'nnTTC
Cuts at: 371 5551 7145 7211 371
Size: 5180 1594 66 4724
Fragments arranged by size:
5180 4724 1594 66
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AscI AvrII BmtI BsiWI BssHII BstZ17I
ClaI FseI FspAI MfeI NheI NotI NruI PacI
PmlI PsiI PsrI SgrAI SnaBI SrfI SwaI ZraI