Restriction map for
pF1KE3826
(Circular) (Six-base) MAPSORT of: pF1KE3826.seq Check: 2012 from: 1 to: 11126
pF1KE3826 11126 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 882 1372 3863 3980 4081 4148 5393 6323 7387
Size: 490 2491 117 101 67 1245 930 1064
Cuts at: 7387 882
Size: 4621
Fragments arranged by size:
4621 2491 1245 1064 930 490 117 101
67
AatII G_ACGT'C
Cuts at: 6191 6191
Size: 11126
AccI GT'mk_AC
Cuts at: 4250 4618 5573 7247 7304 8164 4250
Size: 368 955 1674 57 860 7212
Fragments arranged by size:
7212 1674 955 860 368 57
Acc65I G'GTAC_C
Cuts at: 2297 8142 2297
Size: 5845 5281
AclI AA'CG_TT
Cuts at: 10966 10966
Size: 11126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1693 2326 2724 2898 3016 3034 3336 3718 4530
Size: 633 398 174 118 18 302 382 812
Cuts at: 4530 4945 6340 8951 9383 10130 10398 1693
Size: 415 1395 2611 432 747 268 2421
Fragments arranged by size:
2611 2421 1395 812 747 633 432 415
398 382 302 268 174 118 18
AfeI AGC'GCT
Cuts at: 5017 5458 6880 5017
Size: 441 1422 9263
Fragments arranged by size:
9263 1422 441
AflII C'TTAA_G
Cuts at: 7708 7708
Size: 11126
AflIII A'CryG_T
Cuts at: 865 1292 1760 2567 5676 6467 7799 8478 9582
Size: 427 468 807 3109 791 1332 679 1104
Cuts at: 9582 10559 865
Size: 977 1432
Fragments arranged by size:
3109 1432 1332 1104 977 807 791 679
468 427
AgeI A'CCGG_T
Cuts at: 9514 9514
Size: 11126
AhdI GACnn_n'nnGTC
Cuts at: 4296 5448 4296
Size: 1152 9974
AleI CACnn'nnGTG
Cuts at: 1312 2887 4330 4381 5704 6802 1312
Size: 1575 1443 51 1323 1098 5636
Fragments arranged by size:
5636 1575 1443 1323 1098 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1330 2089 2860 3289 3323 4291 4594 5116 5179
Size: 759 771 429 34 968 303 522 63
Cuts at: 5179 5191 5722 1330
Size: 12 531 6734
Fragments arranged by size:
6734 968 771 759 531 522 429 303
63 34 12
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1978 2010 4558 4590 5188 5220 6454 6486 7156
Size: 32 2548 32 598 32 1234 32 670
Cuts at: 7156 7188 1978
Size: 32 5916
Fragments arranged by size:
5916 2548 1234 670 598 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 384 480 687 1533 1629 2370 2484 3148 3399
Size: 96 207 846 96 741 114 664 251
Cuts at: 3399 3801 3840 4005 4611 5124 5745 5838 6180
Size: 402 39 165 606 513 621 93 342
Cuts at: 6180 6315 6546 8431 9998 384
Size: 135 231 1885 1567 1512
Fragments arranged by size:
1885 1567 1512 846 741 664 621 606
513 402 342 251 231 207 165 135
114 96 96 93 39
ApaI G_GGCC'C
Cuts at: 2801 5496 5644 5959 6947 7864 2801
Size: 2695 148 315 988 917 6063
Fragments arranged by size:
6063 2695 988 917 315 148
ApaLI G'TGCA_C
Cuts at: 2458 3967 4266 9896 2458
Size: 1509 299 5630 3688
Fragments arranged by size:
5630 3688 1509 299
ApoI r'AATT_y
Cuts at: 7063 8130 10479 10735 7063
Size: 1067 2349 256 7454
Fragments arranged by size:
7454 2349 1067 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 11126
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11126
AvaI C'yCGr_G
Cuts at: 2573 3321 4454 4633 7297 7583 8146 2573
Size: 748 1133 179 2664 286 563 5553
Fragments arranged by size:
5553 2664 1133 748 563 286 179
AvrII C'CTAG_G
Cuts at: 8085 8085
Size: 11126
BamHI G'GATC_C
Cuts at: 6839 8151 6839
Size: 1312 9814
BanI G'GyrC_C
Cuts at: 1277 1360 2297 3325 3532 3989 6964 7021 7419
Size: 83 937 1028 207 457 2975 57 398
Cuts at: 7419 8142 8556 8804 8839 1277
Size: 723 414 248 35 3564
Fragments arranged by size:
3564 2975 1028 937 723 457 414 398
248 207 83 57 35
BanII G_rGCy'C
Cuts at: 169 227 710 1991 2060 2801 3881 4669 5496
Size: 58 483 1281 69 741 1080 788 827
Cuts at: 5496 5644 5888 5959 6244 6947 7415 7864 8045
Size: 148 244 71 285 703 468 449 181
Cuts at: 8045 8140 9170 169
Size: 95 1030 2125
Fragments arranged by size:
2125 1281 1080 1030 827 788 741 703
483 468 449 285 244 181 148 95
71 69 58
BbeI G_GCGC'C
Cuts at: 3536 3993 8560 8808 3536
Size: 457 4567 248 5854
Fragments arranged by size:
5854 4567 457 248
BbsI GAAGACnn'nnnn_
Cuts at: 1544 2904 3078 3232 5140 6446 1544
Size: 1360 174 154 1908 1306 6224
Fragments arranged by size:
6224 1908 1360 1306 174 154
BbvCI CC'TCA_GC
Cuts at: 1058 6414 1058
Size: 5356 5770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1176 1210 4584 4618 1176
Size: 34 3374 34 7684
Fragments arranged by size:
7684 3374 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9024 9785 11126 9024
Size: 761 1341 9024
Fragments arranged by size:
9024 1341 761
BclI T'GATC_A
Cuts at: 3434 4067 7886 8646 3434
Size: 633 3819 760 5914
Fragments arranged by size:
5914 3819 760 633
BglI GCCn_nnn'nGGC
Cuts at: 931 1130 1347 2248 4702 7231 931
Size: 199 217 901 2454 2529 4826
Fragments arranged by size:
4826 2529 2454 901 217 199
BglII A'GATC_T
Cuts at: 2357 4397 5351 8641 10678 2357
Size: 2040 954 3290 2037 2805
Fragments arranged by size:
3290 2805 2040 2037 954
BlpI GC'TnA_GC
Cuts at: 709 2284 2893 2951 4513 4762 4943 6146 6373
Size: 1575 609 58 1562 249 181 1203 227
Cuts at: 6373 8238 709
Size: 1865 3597
Fragments arranged by size:
3597 1865 1575 1562 1203 609 249 227
181 58
Bme1580I G_kGCm'C
Cuts at: 289 1004 1119 1253 1319 1363 1412 2462 2657
Size: 715 115 134 66 44 49 1050 195
Cuts at: 2657 2801 2873 2922 3330 3971 4270 5496 5644
Size: 144 72 49 408 641 299 1226 148
Cuts at: 5644 5663 5701 5959 6530 6947 6969 7864 8751
Size: 19 38 258 571 417 22 895 887
Cuts at: 8751 8844 9900 289
Size: 93 1056 1515
Fragments arranged by size:
1515 1226 1056 1050 895 887 715 641
571 417 408 299 258 195 148 144
134 115 93 72 66 49 49 44
38 22 19
BmgBI CAC'GTC
Cuts at: 825 866 3417 4293 6470 825
Size: 41 2551 876 2177 5481
Fragments arranged by size:
5481 2551 2177 876 41
BmrI ACTGGGnnnn_n'
Cuts at: 1064 5274 6517 8338 8419 8754 10898 1064
Size: 4210 1243 1821 81 335 2144 1292
Fragments arranged by size:
4210 2144 1821 1292 1243 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6378 9493 6378
Size: 3115 8011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 820 1741 3262 3904 4306 4791 4933 5014
Size: 264 921 1521 642 402 485 142 81
Cuts at: 5014 5176 6273 7756 7810 7966 556
Size: 162 1097 1483 54 156 3716
Fragments arranged by size:
3716 1521 1483 1097 921 642 485 402
264 162 156 142 81 54
Bpu10I CC'TnA_GC
Cuts at: 1058 2155 4979 6414 1058
Size: 1097 2824 1435 5770
Fragments arranged by size:
5770 2824 1435 1097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5113 6067 6391 7264 8029 8302 9181 9673 9971
Size: 954 324 873 765 273 879 492 298
Cuts at: 9971 5113
Size: 6268
Fragments arranged by size:
6268 954 879 873 765 492 324 298
273
BsaI GGTCTCn'nnnn_
Cuts at: 1421 1948 2998 3324 3886 5497 5926 10830 1421
Size: 527 1050 326 562 1611 429 4904 1717
Fragments arranged by size:
4904 1717 1611 1050 562 527 429 326
BsaAI yAC'GTr
Cuts at: 1295 2192 2457 2463 4371 4509 6247 7077 7875
Size: 897 265 6 1908 138 1738 830 798
Cuts at: 7875 8479 1295
Size: 604 3942
Fragments arranged by size:
3942 1908 1738 897 830 798 604 265
138 6
BsaBI GATnn'nnATC
Cuts at: 6 3775 6844 7489 8640 10677 6
Size: 3769 3069 645 1151 2037 455
Fragments arranged by size:
3769 3069 2037 1151 645 455
BsaHI Gr'CG_yC
Cuts at: 1448 3533 3990 6188 8557 8805 9507 11003 1448
Size: 2085 457 2198 2369 248 702 1496 1571
Fragments arranged by size:
2369 2198 2085 1571 1496 702 457 248
BsaWI w'CCGG_w
Cuts at: 2105 4685 5198 8315 8836 9514 9788 9935 10346
Size: 2580 513 3117 521 678 274 147 411
Cuts at: 10346 2105
Size: 2885
Fragments arranged by size:
3117 2885 2580 678 521 513 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 133 163 793 812 823 842 1438 1468 1714
Size: 30 630 19 11 19 596 30 246
Cuts at: 1714 1744 1978 2008 2344 2374 3196 3226 4651
Size: 30 234 30 336 30 822 30 1425
Cuts at: 4651 4681 5074 5104 5494 5524 6079 6109 6616
Size: 30 393 30 390 30 555 30 507
Cuts at: 6616 6646 6682 6712 6778 6808 7156 7186 7240
Size: 30 36 30 66 30 348 30 54
Cuts at: 7240 7270 7309 7339 133
Size: 30 39 30 3920
Fragments arranged by size:
3920 1425 822 630 596 555 507 393
390 348 336 246 234 66 54 39
36 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 817 1007 1075 1345 1348 1738 2683 2992 3532
Size: 190 68 270 3 390 945 309 540
Cuts at: 3532 3583 4621 4930 5095 5610 6358 6361 6445
Size: 51 1038 309 165 515 748 3 84
Cuts at: 6445 6448 7177 7714 817
Size: 3 729 537 4229
Fragments arranged by size:
4229 1038 945 748 729 540 537 515
390 309 309 270 190 165 84 68
51 3 3 3
BseYI C'CCAG_C
Cuts at: 997 1144 1356 2363 2370 2772 2923 2935 4793
Size: 147 212 1007 7 402 151 12 1858
Cuts at: 4793 5495 5637 5644 5880 7578 8030 8551 9309
Size: 702 142 7 236 1698 452 521 758
Cuts at: 9309 9886 10382 997
Size: 577 496 1741
Fragments arranged by size:
1858 1741 1698 1007 758 702 577 521
496 452 402 236 212 151 147 142
12 7 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1269 1888 2437 2812 3565 3829 3836 3841
Size: 803 619 549 375 753 264 7 5
Cuts at: 3841 4000 4018 4624 4802 4954 5458 5701 6625
Size: 159 18 606 178 152 504 243 924
Cuts at: 6625 466
Size: 4967
Fragments arranged by size:
4967 924 803 753 619 606 549 504
375 264 243 178 159 152 18 7
5
BsiEI CG_ry'CG
Cuts at: 104 233 3264 5481 7308 8668 8714 9922 104
Size: 129 3031 2217 1827 1360 46 1208 1308
Fragments arranged by size:
3031 2217 1827 1360 1308 1208 129 46
BsiHKAI G_wGCw'C
Cuts at: 710 1309 1826 2060 2285 2462 3284 3971 4270
Size: 599 517 234 225 177 822 687 299
Cuts at: 4270 5711 7796 8045 8140 8918 9108 9900 710
Size: 1441 2085 249 95 778 190 792 1936
Fragments arranged by size:
2085 1936 1441 822 792 778 687 599
517 299 249 234 225 190 177 95
BsiWI C'GTAC_G
Cuts at: 1262 1262
Size: 11126
BsmI GAATG_Cn'
Cuts at: 1146 1667 1791 6335 1146
Size: 521 124 4544 5937
Fragments arranged by size:
5937 4544 521 124
BsmBI CGTCTCn'nnnn_
Cuts at: 153 815 2515 5818 7159 153
Size: 662 1700 3303 1341 4120
Fragments arranged by size:
4120 3303 1700 1341 662
Bsp1286I G_dGCh'C
Cuts at: 169 227 289 710 1004 1119 1253 1309 1319
Size: 58 62 421 294 115 134 56 10
Cuts at: 1319 1363 1412 1826 1991 2060 2285 2462 2657
Size: 44 49 414 165 69 225 177 195
Cuts at: 2657 2801 2873 2922 3284 3330 3881 3971 4270
Size: 144 72 49 362 46 551 90 299
Cuts at: 4270 4669 5496 5644 5663 5701 5711 5888 5959
Size: 399 827 148 19 38 10 177 71
Cuts at: 5959 6244 6530 6947 6969 7415 7796 7864 8045
Size: 285 286 417 22 446 381 68 181
Cuts at: 8045 8140 8751 8844 8918 9108 9170 9900 169
Size: 95 611 93 74 190 62 730 1395
Fragments arranged by size:
1395 827 730 611 551 446 421 417
414 399 381 362 299 294 286 285
225 195 190 181 177 177 165 148
144 134 115 95 93 90 74 72
71 69 68 62 62 58 56 49
49 46 44 38 22 19 10 10
BspEI T'CCGG_A
Cuts at: 2105 4685 10346 2105
Size: 2580 5661 2885
Fragments arranged by size:
5661 2885 2580
BspHI T'CATG_A
Cuts at: 2217 4070 7116 7502 10302 2217
Size: 1853 3046 386 2800 3041
Fragments arranged by size:
3046 3041 2800 1853 386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 882 1372 1505 2432 3083 3378 3740 3863 3980
Size: 490 133 927 651 295 362 123 117
Cuts at: 3980 4081 4148 4226 5393 5777 6215 6323 7387
Size: 101 67 78 1167 384 438 108 1064
Cuts at: 7387 7682 8176 8390 8692 9073 882
Size: 295 494 214 302 381 2935
Fragments arranged by size:
2935 1167 1064 927 651 494 490 438
384 381 362 302 295 295 214 133
123 117 108 101 78 67
BsrBI CCG'CTC
Cuts at: 50 1338 4920 7208 7464 9418 9472 10956 11121
Size: 1288 3582 2288 256 1954 54 1484 165
Cuts at: 11121 50
Size: 55
Fragments arranged by size:
3582 2288 1954 1484 1288 256 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1194 1826 6761 6826 7206 7950 9038 1194
Size: 632 4935 65 380 744 1088 3282
Fragments arranged by size:
4935 3282 1088 744 632 380 65
BsrFI r'CCGG_y
Cuts at: 721 2257 2493 3452 5285 6125 6324 6566 7307
Size: 1536 236 959 1833 840 199 242 741
Cuts at: 7307 9124 9305 9514 721
Size: 1817 181 209 2333
Fragments arranged by size:
2333 1833 1817 1536 959 840 741 242
236 209 199 181
BssHII G'CGCG_C
Cuts at: 2427 2427
Size: 11126
BssSI C'ACGA_G
Cuts at: 2146 5662 5992 9397 9755 2146
Size: 3516 330 3405 358 3517
Fragments arranged by size:
3517 3516 3405 358 330
BstAPI GCAn_nnn'nTGC
Cuts at: 384 687 3693 3966 4611 5121 5124 6315 8503
Size: 303 3006 273 645 510 3 1191 2188
Cuts at: 8503 384
Size: 3007
Fragments arranged by size:
3007 3006 2188 1191 645 510 303 273
3
BstBI TT'CG_AA
Cuts at: 9487 9487
Size: 11126
BstXI CCAn_nnnn'nTGG
Cuts at: 1473 1998 2607 3771 4737 5313 7053 7101 7341
Size: 525 609 1164 966 576 1740 48 240
Cuts at: 7341 8010 1473
Size: 669 4589
Fragments arranged by size:
4589 1740 1164 966 669 609 576 525
240 48
BstYI r'GATC_y
Cuts at: 1 1092 1421 2357 2522 3017 3740 3776 4397
Size: 1091 329 936 165 495 723 36 621
Cuts at: 4397 5351 5408 6066 6839 8151 8618 8641 8976
Size: 954 57 658 773 1312 467 23 335
Cuts at: 8976 9222 10234 10320 10332 10678 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1312 1091 1012 954 936 773 723 658
621 495 467 449 346 335 329 246
165 86 57 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 7424 7424
Size: 11126
BtgI C'CryG_G
Cuts at: 227 987 1472 2606 2885 3464 3560 4642 4670
Size: 760 485 1134 279 579 96 1082 28
Cuts at: 4670 4697 4730 5513 5861 7017 7046 7509 227
Size: 27 33 783 348 1156 29 463 3844
Fragments arranged by size:
3844 1156 1134 1082 783 760 579 485
463 348 279 96 33 29 28 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1179 1574 2019 4112 6843 9076 9257 9420 1179
Size: 395 445 2093 2731 2233 181 163 2885
Fragments arranged by size:
2885 2731 2233 2093 445 395 181 163
BtsI GCAGTG_nn'
Cuts at: 471 910 1892 2767 3164 3510 3609 4362 4773
Size: 439 982 875 397 346 99 753 411
Cuts at: 4773 5007 5694 7389 8024 8369 471
Size: 234 687 1695 635 345 3228
Fragments arranged by size:
3228 1695 982 875 753 687 635 439
411 397 346 345 234 99
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 7509 7544 7509
Size: 35 11091
DraI TTT'AAA
Cuts at: 8125 8125
Size: 11126
DraIII CAC_nnn'GTG
Cuts at: 3966 5282 5667 3966
Size: 1316 385 9425
Fragments arranged by size:
9425 1316 385
DrdI GACnn_nn'nnGTC
Cuts at: 637 1597 4102 5994 8832 9690 637
Size: 960 2505 1892 2838 858 2073
Fragments arranged by size:
2838 2505 2073 1892 960 858
EaeI y'GGCC_r
Cuts at: 230 932 1463 1877 2345 2804 3261 3977 4223
Size: 702 531 414 468 459 457 716 246
Cuts at: 4223 4703 5375 6740 7203 8711 8885 9276 9303
Size: 480 672 1365 463 1508 174 391 27
Cuts at: 9303 10648 230
Size: 1345 708
Fragments arranged by size:
1508 1365 1345 716 708 702 672 531
480 468 463 459 457 414 391 246
174 27
EagI C'GGCC_G
Cuts at: 230 3261 8711 230
Size: 3031 5450 2645
Fragments arranged by size:
5450 3031 2645
EarI CTCTTCn'nnn_
Cuts at: 280 484 598 2168 2218 3346 3868 4993 6212
Size: 204 114 1570 50 1128 522 1125 1219
Cuts at: 6212 6369 6577 6838 7258 7279 7430 7579 9149
Size: 157 208 261 420 21 151 149 1570
Cuts at: 9149 9359 280
Size: 210 2047
Fragments arranged by size:
2047 1570 1570 1219 1128 1125 522 420
261 210 208 204 157 151 149 114
50 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1756 3040 3217 6880 9334 9644 9790 10936 1756
Size: 1284 177 3663 2454 310 146 1146 1946
Fragments arranged by size:
3663 2454 1946 1284 1146 310 177 146
EcoICRI GAG'CTC
Cuts at: 708 2058 8043 8138 708
Size: 1350 5985 95 3696
Fragments arranged by size:
5985 3696 1350 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 820 1693 1741 2326 2724 2898 3016 3034
Size: 264 873 48 585 398 174 118 18
Cuts at: 3034 3262 3336 3718 3904 4306 4530 4791 4933
Size: 228 74 382 186 402 224 261 142
Cuts at: 4933 4945 5014 5176 6273 6340 7756 7810 7966
Size: 12 69 162 1097 67 1416 54 156
Cuts at: 7966 8951 9383 10130 10398 556
Size: 985 432 747 268 1284
Fragments arranged by size:
1416 1284 1097 985 873 747 585 432
402 398 382 268 264 261 228 224
186 174 162 156 142 118 74 69
67 54 48 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 277 802 1654 1879 4936 6211 6550 7435 277
Size: 525 852 225 3057 1275 339 885 3968
Fragments arranged by size:
3968 3057 1275 885 852 525 339 225
EcoO109I rG'GnC_Cy
Cuts at: 237 1677 1705 2797 2798 2826 3837 5064 5220
Size: 1440 28 1092 1 28 1011 1227 156
Cuts at: 5220 5641 5766 5955 5956 7428 7861 8265 237
Size: 421 125 189 1 1472 433 404 3098
Fragments arranged by size:
3098 1472 1440 1227 1092 1011 433 421
404 189 156 125 28 28 1 1
EcoRI G'AATT_C
Cuts at: 7063 8130 7063
Size: 1067 10059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1407 7305 9145 1407
Size: 5898 1840 3388
Fragments arranged by size:
5898 3388 1840
FspI TGC'GCA
Cuts at: 1975 5167 8907 1975
Size: 3192 3740 4194
Fragments arranged by size:
4194 3740 3192
HaeII r_GCGC'y
Cuts at: 3363 3536 3993 5019 5460 6280 6421 6882 6910
Size: 173 457 1026 441 820 141 461 28
Cuts at: 6910 8560 8808 9830 10809 3363
Size: 1650 248 1022 979 3680
Fragments arranged by size:
3680 1650 1026 1022 979 820 461 457
441 248 173 141 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 648 680 1183 1215 1438 1470 1714
Size: 32 483 32 503 32 223 32 244
Cuts at: 1714 1746 1993 2025 2344 2376 4111 4143 4282
Size: 32 247 32 319 32 1735 32 139
Cuts at: 4282 4314 5434 5466 5494 5526 6079 6111 6616
Size: 32 1120 32 28 32 553 32 505
Cuts at: 6616 6648 6778 6810 7390 7422 8968 8998 9000
Size: 32 130 32 580 32 1546 30 2
Cuts at: 9000 9030 133
Size: 30 2229
Fragments arranged by size:
2229 1735 1546 1120 580 553 505 503
483 319 247 244 223 139 130 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 5526 5574 7305 8165 5526
Size: 48 1731 860 8487
Fragments arranged by size:
8487 1731 860 48
KasI G'GCGC_C
Cuts at: 3532 3989 8556 8804 3532
Size: 457 4567 248 5854
Fragments arranged by size:
5854 4567 457 248
KpnI G_GTAC'C
Cuts at: 2301 8146 2301
Size: 5845 5281
MluI A'CGCG_T
Cuts at: 2567 10559 2567
Size: 7992 3134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2582 3422 5057 9797 9981 2051
Size: 531 840 1635 4740 184 3196
Fragments arranged by size:
4740 3196 1635 840 531 184
MscI TGG'CCA
Cuts at: 1465 2347 3979 4705 5377 8887 1465
Size: 882 1632 726 672 3510 3704
Fragments arranged by size:
3704 3510 1632 882 726 672
MslI CAynn'nnrTG
Cuts at: 446 553 1007 1187 1312 2887 2995 3305 4069
Size: 107 454 180 125 1575 108 310 764
Cuts at: 4069 4330 4381 5098 5704 6134 6802 7051 7057
Size: 261 51 717 606 430 668 249 6
Cuts at: 7057 7501 7627 9115 9242 446
Size: 444 126 1488 127 2330
Fragments arranged by size:
2330 1575 1488 764 717 668 606 454
444 430 310 261 249 180 127 126
125 108 107 51 6
MspA1I CmG'CkG
Cuts at: 212 229 761 1530 2238 2304 2370 2958 3315
Size: 17 532 769 708 66 66 588 357
Cuts at: 3315 3336 3396 4284 4608 4778 4786 4797 5163
Size: 21 60 888 324 170 8 11 366
Cuts at: 5163 5742 6306 6393 6684 8067 8237 8551 8911
Size: 579 564 87 291 1383 170 314 360
Cuts at: 8911 9924 10169 212
Size: 1013 245 1169
Fragments arranged by size:
1383 1169 1013 888 769 708 588 579
564 532 366 360 357 324 314 291
245 170 170 87 66 66 60 21
17 11 8
NaeI GCC'GGC
Cuts at: 723 2259 5287 6127 9307 723
Size: 1536 3028 840 3180 2542
Fragments arranged by size:
3180 3028 2542 1536 840
NarI GG'CG_CC
Cuts at: 3533 3990 8557 8805 3533
Size: 457 4567 248 5854
Fragments arranged by size:
5854 4567 457 248
NcoI C'CATG_G
Cuts at: 2606 2885 3464 4697 4730 7046 7509 2606
Size: 279 579 1233 33 2316 463 6223
Fragments arranged by size:
6223 2316 1233 579 463 279 33
NgoMIV G'CCGG_C
Cuts at: 721 2257 5285 6125 9305 721
Size: 1536 3028 840 3180 2542
Fragments arranged by size:
3180 3028 2542 1536 840
NotI GC'GGCC_GC
Cuts at: 3261 3261
Size: 11126
NspI r_CATG'y
Cuts at: 394 694 1764 2325 3240 3270 6681 7721 7803
Size: 300 1070 561 915 30 3411 1040 82
Cuts at: 7803 8179 8679 9586 394
Size: 376 500 907 1934
Fragments arranged by size:
3411 1934 1070 1040 915 907 561 500
376 300 82 30
PasI CC'CwG_GG
Cuts at: 7341 7341
Size: 11126
PciI A'CATG_T
Cuts at: 1760 7799 9582 1760
Size: 6039 1783 3304
Fragments arranged by size:
6039 3304 1783
PflMI CCAn_nnn'nTGG
Cuts at: 1295 1533 2343 2370 2484 2865 4143 4862 5388
Size: 238 810 27 114 381 1278 719 526
Cuts at: 5388 5928 6180 1295
Size: 540 252 6241
Fragments arranged by size:
6241 1278 810 719 540 526 381 252
238 114 27
PfoI T'CCnGG_A
Cuts at: 1586 1922 1997 3020 3632 3881 4991 5357 5837
Size: 336 75 1023 612 249 1110 366 480
Cuts at: 5837 7430 7982 1586
Size: 1593 552 4730
Fragments arranged by size:
4730 1593 1110 1023 612 552 480 366
336 249 75
PmeI GTTT'AAAC
Cuts at: 8125 8125
Size: 11126
PmlI CAC'GTG
Cuts at: 1295 2192 2457 2463 6247 7077 1295
Size: 897 265 6 3784 830 5344
Fragments arranged by size:
5344 3784 897 830 265 6
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 286 318 1978 2010 7156 7188 10265 10297 286
Size: 32 1660 32 5146 32 3077 32 1115
Fragments arranged by size:
5146 3077 1660 1115 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 237 1705 2826 5064 5220 5766 7428 237
Size: 1468 1121 2238 156 546 1662 3935
Fragments arranged by size:
3935 2238 1662 1468 1121 546 156
PshAI GACnn'nnGTC
Cuts at: 1960 7096 1960
Size: 5136 5990
PspOMI G'GGCC_C
Cuts at: 2797 5492 5640 5955 6943 7860 2797
Size: 2695 148 315 988 917 6063
Fragments arranged by size:
6063 2695 988 917 315 148
PspXI vC'TCGA_Gb
Cuts at: 4633 4633
Size: 11126
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1057 1089 1735 1767 4351 4383 7798 7830 1057
Size: 32 646 32 2584 32 3415 32 4353
Fragments arranged by size:
4353 3415 2584 646 32 32 32 32
PstI C_TGCA'G
Cuts at: 380 389 893 2816 3335 3740 3806 4465 4583
Size: 9 504 1923 519 405 66 659 118
Cuts at: 4583 4616 4895 5117 5741 5774 5834 6080 6212
Size: 33 279 222 624 33 60 246 132
Cuts at: 6212 6284 6311 7384 8173 8858 380
Size: 72 27 1073 789 685 2648
Fragments arranged by size:
2648 1923 1073 789 685 659 624 519
504 405 279 246 222 132 118 72
66 60 33 33 27 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11126
PvuII CAG'CTG
Cuts at: 761 1530 2238 2304 2370 3336 3396 4608 4778
Size: 769 708 66 66 966 60 1212 170
Cuts at: 4778 4786 5163 5742 6306 6393 8551 8911 761
Size: 8 377 579 564 87 2158 360 2976
Fragments arranged by size:
2976 2158 1212 966 769 708 579 564
377 360 170 87 66 66 60 8
RsrII CG'GwC_CG
Cuts at: 2108 9321 2108
Size: 7213 3913
SacI G_AGCT'C
Cuts at: 710 2060 8045 8140 710
Size: 1350 5985 95 3696
Fragments arranged by size:
5985 3696 1350 95
SacII CC_GC'GG
Cuts at: 230 230
Size: 11126
SalI G'TCGA_C
Cuts at: 5572 7303 8163 5572
Size: 1731 860 8535
Fragments arranged by size:
8535 1731 860
SapI GCTCTTCn'nnn_
Cuts at: 484 598 3346 4993 6212 6577 6838 9149 9359
Size: 114 2748 1647 1219 365 261 2311 210
Cuts at: 9359 484
Size: 2251
Fragments arranged by size:
2748 2311 2251 1647 1219 365 261 210
114
SbfI CC_TGCA'GG
Cuts at: 6212 8173 6212
Size: 1961 9165
ScaI AGT'ACT
Cuts at: 319 1162 2167 2341 5947 10643 319
Size: 843 1005 174 3606 4696 802
Fragments arranged by size:
4696 3606 1005 843 802 174
SexAI A'CCwGG_T
Cuts at: 1541 1541
Size: 11126
SfcI C'TryA_G
Cuts at: 33 376 385 889 1037 2292 2733 2812 3331
Size: 343 9 504 148 1255 441 79 519
Cuts at: 3331 3736 3802 4461 4579 4612 4648 4891 5113
Size: 405 66 659 118 33 36 243 222
Cuts at: 5113 5737 5770 5830 6076 6208 6280 6307 6882
Size: 624 33 60 246 132 72 27 575
Cuts at: 6882 7128 7380 8169 8854 9847 10038 33
Size: 246 252 789 685 993 191 1121
Fragments arranged by size:
1255 1121 993 789 685 659 624 575
519 504 441 405 343 252 246 246
243 222 191 148 132 118 79 72
66 60 36 33 33 27 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 1130 1130
Size: 11126
SfoI GGC'GCC
Cuts at: 3534 3991 8558 8806 3534
Size: 457 4567 248 5854
Fragments arranged by size:
5854 4567 457 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11126
SmaI CCC'GGG
Cuts at: 8148 8148
Size: 11126
SmlI C'TyrA_G
Cuts at: 4633 5128 6082 6406 7279 7297 7708 8044 8281
Size: 495 954 324 873 18 411 336 237
Cuts at: 8281 9196 9688 9950 4633
Size: 915 492 262 5809
Fragments arranged by size:
5809 954 915 873 495 492 411 336
324 262 237 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11126
SphI G_CATG'C
Cuts at: 394 694 3240 6681 8179 8679 394
Size: 300 2546 3441 1498 500 2841
Fragments arranged by size:
3441 2841 2546 1498 500 300
StuI AGG'CCT
Cuts at: 1390 2818 4678 5008 6202 1390
Size: 1428 1860 330 1194 6314
Fragments arranged by size:
6314 1860 1428 1194 330
StyI C'CwwG_G
Cuts at: 1097 1373 2606 2885 3464 3539 4496 4697 4706
Size: 276 1233 279 579 75 957 201 9
Cuts at: 4706 4730 5060 5927 7046 7509 8085 8260 8614
Size: 24 330 867 1119 463 576 175 354
Cuts at: 8614 1097
Size: 3609
Fragments arranged by size:
3609 1233 1119 957 867 579 576 463
354 330 279 276 201 175 75 24
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1960 2065 2125 3477 3599 5293 6952 7864 8709
Size: 105 60 1352 122 1694 1659 912 845
Cuts at: 8709 8842 9301 9510 1960
Size: 133 459 209 3576
Fragments arranged by size:
3576 1694 1659 1352 912 845 459 209
133 122 105 60
TatI w'GTAC_w
Cuts at: 317 875 1160 2165 2339 5945 7814 10641 317
Size: 558 285 1005 174 3606 1869 2827 802
Fragments arranged by size:
3606 2827 1869 1005 802 558 285 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 812 844 2863 2895 4651 4683 5074 5106 5833
Size: 32 2019 32 1756 32 391 32 727
Cuts at: 5833 5865 6941 6973 10643 10675 812
Size: 32 1076 32 3670 32 1263
Fragments arranged by size:
3670 2019 1756 1263 1076 727 391 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 145 5508 7402 7764 8923 145
Size: 5363 1894 362 1159 2348
Fragments arranged by size:
5363 2348 1894 1159 362
XbaI T'CTAG_A
Cuts at: 8157 8157
Size: 11126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1458 1947 2613 2862 3177 3216 5067 5385 5389
Size: 489 666 249 315 39 1851 318 4
Cuts at: 5389 5514 5592 5811 5868 6105 6804 6930 8007
Size: 125 78 219 57 237 699 126 1077
Cuts at: 8007 8621 1458
Size: 614 3963
Fragments arranged by size:
3963 1851 1077 699 666 614 489 318
315 249 237 219 126 125 78 57
39 4
XhoI C'TCGA_G
Cuts at: 4633 7297 4633
Size: 2664 8462
XmaI C'CCGG_G
Cuts at: 8146 8146
Size: 11126
XmnI GAAnn'nnTTC
Cuts at: 3029 5353 5419 3029
Size: 2324 66 8736
Fragments arranged by size:
8736 2324 66
ZraI GAC'GTC
Cuts at: 6189 6189
Size: 11126
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BmtI BsrGI BstEII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI NsiI PacI PsiI SanDI SgrAI SnaBI SrfI
SspI SwaI