Restriction map for
pF1KE3815
(Circular) (Six-base) MAPSORT of: pF1KE3815.seq Check: 474 from: 1 to: 4568
pF1KE3815 4568 bp
With 182 enzymes: SgfI *
July 24, 2018 16:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 204 204
Size: 4568
AccI GT'mk_AC
Cuts at: 1606 1606
Size: 4568
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2825 3572 3840 2393
Size: 432 747 268 3121
Fragments arranged by size:
3121 747 432 268
AflIII A'CryG_T
Cuts at: 1920 3024 4001 1920
Size: 1104 977 2487
Fragments arranged by size:
2487 1104 977
AgeI A'CCGG_T
Cuts at: 2956 2956
Size: 4568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1413 1413
Size: 4568
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 843 875 843
Size: 32 4536
AlwNI CAG_nnn'CTG
Cuts at: 1873 3440 1873
Size: 1567 3001
ApaI G_GGCC'C
Cuts at: 1129 1154 1129
Size: 25 4543
ApaLI G'TGCA_C
Cuts at: 205 223 3338 205
Size: 18 3115 1435
Fragments arranged by size:
3115 1435 18
ApoI r'AATT_y
Cuts at: 268 1572 3921 4177 268
Size: 1304 2349 256 659
Fragments arranged by size:
2349 1304 659 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 335 372 420 547 659 738 1077 1476 1588
Size: 37 48 127 112 79 339 399 112
Cuts at: 1588 335
Size: 3315
Fragments arranged by size:
3315 399 339 127 112 112 79 48
37
BamHI G'GATC_C
Cuts at: 598 1593 598
Size: 995 3573
BanI G'GyrC_C
Cuts at: 734 756 1350 1377 1584 1998 2246 2281 734
Size: 22 594 27 207 414 248 35 3021
Fragments arranged by size:
3021 594 414 248 207 35 27 22
BanII G_rGCy'C
Cuts at: 235 284 473 894 1129 1154 1421 1432 1548
Size: 49 189 421 235 25 267 11 116
Cuts at: 1548 1582 2612 235
Size: 34 1030 2191
Fragments arranged by size:
2191 1030 421 267 235 189 116 49
34 25 11
BbeI G_GCGC'C
Cuts at: 760 1354 1381 2002 2250 760
Size: 594 27 621 248 3078
Fragments arranged by size:
3078 621 594 248 27
BbvCI CC'TCA_GC
Cuts at: 218 514 1525 218
Size: 296 1011 3261
Fragments arranged by size:
3261 1011 296
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3227 4568 2466
Size: 761 1341 2466
Fragments arranged by size:
2466 1341 761
BclI T'GATC_A
Cuts at: 2088 2088
Size: 4568
BglI GCCn_nnn'nGGC
Cuts at: 964 1021 1124 1252 964
Size: 57 103 128 4280
Fragments arranged by size:
4280 128 103 57
BglII A'GATC_T
Cuts at: 707 2083 4120 707
Size: 1376 2037 1155
Fragments arranged by size:
2037 1376 1155
BlpI GC'TnA_GC
Cuts at: 1680 1680
Size: 4568
Bme1580I G_kGCm'C
Cuts at: 209 227 878 1129 1154 2193 2286 3342 209
Size: 18 651 251 25 1039 93 1056 1435
Fragments arranged by size:
1435 1056 1039 651 251 93 25 18
BmgBI CAC'GTC
Cuts at: 1199 1199
Size: 4568
BmrI ACTGGGnnnn_n'
Cuts at: 1780 1861 2196 4340 1780
Size: 81 335 2144 2008
Fragments arranged by size:
2144 2008 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 993 1524 2935 993
Size: 531 1411 2626
Fragments arranged by size:
2626 1411 531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1507 1573 1351
Size: 156 66 4346
Fragments arranged by size:
4346 156 66
Bpu10I CC'TnA_GC
Cuts at: 218 514 1525 218
Size: 296 1011 3261
Fragments arranged by size:
3261 1011 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1142 1744 2623 3115 3413 1142
Size: 602 879 492 298 2297
Fragments arranged by size:
2297 879 602 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4272 4272
Size: 4568
BsaAI yAC'GTr
Cuts at: 873 1921 873
Size: 1048 3520
BsaBI GATnn'nnATC
Cuts at: 6 706 2082 4119 6
Size: 700 1376 2037 455
Fragments arranged by size:
2037 1376 700 455
BsaHI Gr'CG_yC
Cuts at: 757 1307 1351 1378 1999 2247 2949 4445 757
Size: 550 44 27 621 248 702 1496 880
Fragments arranged by size:
1496 880 702 621 550 248 44 27
BsaWI w'CCGG_w
Cuts at: 465 595 1029 1757 2278 2956 3230 3377 3788
Size: 130 434 728 521 678 274 147 411
Cuts at: 3788 465
Size: 1245
Fragments arranged by size:
1245 728 678 521 434 411 274 147
130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 679 1016 1049 1570 607
Size: 72 337 33 521 3605
Fragments arranged by size:
3605 521 337 72 33
BseYI C'CCAG_C
Cuts at: 949 1223 1289 1993 2751 3328 3824 949
Size: 274 66 704 758 577 496 1693
Fragments arranged by size:
1693 758 704 577 496 274 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 801 1373 176
Size: 625 572 3371
Fragments arranged by size:
3371 625 572
BsiEI CG_ry'CG
Cuts at: 104 277 2110 2156 3364 104
Size: 173 1833 46 1208 1308
Fragments arranged by size:
1833 1308 1208 173 46
BsiHKAI G_wGCw'C
Cuts at: 209 227 1421 1582 2360 2550 3342 209
Size: 18 1194 161 778 190 792 1435
Fragments arranged by size:
1435 1194 792 778 190 161 18
BsmI GAATG_Cn'
Cuts at: 461 461
Size: 4568
BsmBI CGTCTCn'nnnn_
Cuts at: 546 673 546
Size: 127 4441
Bsp1286I G_dGCh'C
Cuts at: 209 227 235 284 473 878 894 1129 1154
Size: 18 8 49 189 405 16 235 25
Cuts at: 1154 1421 1432 1548 1582 2193 2286 2360 2550
Size: 267 11 116 34 611 93 74 190
Cuts at: 2550 2612 3342 209
Size: 62 730 1435
Fragments arranged by size:
1435 730 611 405 267 235 190 189
116 93 74 62 49 34 25 18
16 11 8
BspEI T'CCGG_A
Cuts at: 465 1029 3788 465
Size: 564 2759 1245
Fragments arranged by size:
2759 1245 564
BspHI T'CATG_A
Cuts at: 3744 3744
Size: 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 204 993 1618 1832 2134 2515 204
Size: 789 625 214 302 381 2257
Fragments arranged by size:
2257 789 625 381 302 214
BsrBI CCG'CTC
Cuts at: 50 408 1156 2860 2914 4398 4563 50
Size: 358 748 1704 54 1484 165 55
Fragments arranged by size:
1704 1484 748 358 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2480 2480
Size: 4568
BsrFI r'CCGG_y
Cuts at: 237 524 572 838 963 1374 2566 2747 2956
Size: 287 48 266 125 411 1192 181 209
Cuts at: 2956 237
Size: 1849
Fragments arranged by size:
1849 1192 411 287 266 209 181 125
48
BssHII G'CGCG_C
Cuts at: 881 994 881
Size: 113 4455
BssSI C'ACGA_G
Cuts at: 816 2839 3197 816
Size: 2023 358 2187
Fragments arranged by size:
2187 2023 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1945 1945
Size: 4568
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstEII G'GTnAC_C
Cuts at: 802 802
Size: 4568
BstYI r'GATC_y
Cuts at: 1 598 707 974 1593 2060 2083 2418 2664
Size: 597 109 267 619 467 23 335 246
Cuts at: 2664 3676 3762 3774 4120 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 619 597 467 449 346 335 267
246 109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1052 1052
Size: 4568
BtgI C'CryG_G
Cuts at: 326 1213 326
Size: 887 3681
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 304 411 636 2518 2699 2862 304
Size: 107 225 1882 181 163 2010
Fragments arranged by size:
2010 1882 225 181 163 107
BtsI GCAGTG_nn'
Cuts at: 209 1811 209
Size: 1602 2966
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3132 2274
Size: 858 3710
EaeI y'GGCC_r
Cuts at: 274 671 2153 2327 2718 2745 4090 274
Size: 397 1482 174 391 27 1345 752
Fragments arranged by size:
1482 1345 752 397 391 174 27
EagI C'GGCC_G
Cuts at: 274 2153 274
Size: 1879 2689
EarI CTCTTCn'nnn_
Cuts at: 1493 2591 2801 1493
Size: 1098 210 3260
Fragments arranged by size:
3260 1098 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1302 2776 3086 3232 4378 1302
Size: 1474 310 146 1146 1492
Fragments arranged by size:
1492 1474 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1419 1580 1419
Size: 161 4407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 1507 1573 2393 2825 3572 3840 1351
Size: 156 66 820 432 747 268 2079
Fragments arranged by size:
2079 820 747 432 268 156 66
EcoNI CCTnn'n_nnAGG
Cuts at: 143 1528 143
Size: 1385 3183
EcoO109I rG'GnC_Cy
Cuts at: 387 1101 1126 1234 1473 1707 387
Size: 714 25 108 239 234 3248
Fragments arranged by size:
3248 714 239 234 108 25
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 657 1201 2587 657
Size: 544 1386 2638
Fragments arranged by size:
2638 1386 544
FspI TGC'GCA
Cuts at: 2349 2349
Size: 4568
HaeII r_GCGC'y
Cuts at: 760 1042 1354 1366 1381 2002 2250 3272 4251
Size: 282 312 12 15 621 248 1022 979
Cuts at: 4251 760
Size: 1077
Fragments arranged by size:
1077 1022 979 621 312 282 248 15
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 2410 2440 2442 2472 344
Size: 32 2034 30 2 30 2440
Fragments arranged by size:
2440 2034 32 30 30 2
HincII GTy'rAC
Cuts at: 1607 1607
Size: 4568
KasI G'GCGC_C
Cuts at: 756 1350 1377 1998 2246 756
Size: 594 27 621 248 3078
Fragments arranged by size:
3078 621 594 248 27
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 4001 4001
Size: 4568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3239 3423 3239
Size: 184 4384
MscI TGG'CCA
Cuts at: 673 2329 673
Size: 1656 2912
MslI CAynn'nnrTG
Cuts at: 289 2557 2684 289
Size: 2268 127 2173
Fragments arranged by size:
2268 2173 127
MspA1I CmG'CkG
Cuts at: 328 513 1679 1993 2353 3366 3611 328
Size: 185 1166 314 360 1013 245 1285
Fragments arranged by size:
1285 1166 1013 360 314 245 185
NaeI GCC'GGC
Cuts at: 239 574 965 1376 2749 239
Size: 335 391 411 1373 2058
Fragments arranged by size:
2058 1373 411 391 335
NarI GG'CG_CC
Cuts at: 757 1351 1378 1999 2247 757
Size: 594 27 621 248 3078
Fragments arranged by size:
3078 621 594 248 27
NgoMIV G'CCGG_C
Cuts at: 237 572 963 1374 2747 237
Size: 335 391 411 1373 2058
Fragments arranged by size:
2058 1373 411 391 335
NspI r_CATG'y
Cuts at: 1621 2121 3028 1621
Size: 500 907 3161
Fragments arranged by size:
3161 907 500
PasI CC'CwG_GG
Cuts at: 473 1203 473
Size: 730 3838
PciI A'CATG_T
Cuts at: 3024 3024
Size: 4568
PfoI T'CCnGG_A
Cuts at: 689 697 1367 689
Size: 8 670 3890
Fragments arranged by size:
3890 670 8
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PmlI CAC'GTG
Cuts at: 873 873
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4536
PpuMI rG'GwC_Cy
Cuts at: 1101 1234 1101
Size: 133 4435
PshAI GACnn'nnGTC
Cuts at: 589 589
Size: 4568
PspOMI G'GGCC_C
Cuts at: 1125 1150 1125
Size: 25 4543
PstI C_TGCA'G
Cuts at: 947 1445 1615 2300 947
Size: 498 170 685 3215
Fragments arranged by size:
3215 685 498 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 513 1993 2353 513
Size: 1480 360 2728
Fragments arranged by size:
2728 1480 360
RsrII CG'GwC_CG
Cuts at: 584 2763 584
Size: 2179 2389
SacI G_AGCT'C
Cuts at: 1421 1582 1421
Size: 161 4407
SacII CC_GC'GG
Cuts at: 329 329
Size: 4568
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SanDI GG'GwC_CC
Cuts at: 1101 1234 1101
Size: 133 4435
SapI GCTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4568
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SfcI C'TryA_G
Cuts at: 33 943 1441 1611 2296 3289 3480 33
Size: 910 498 170 685 993 191 1121
Fragments arranged by size:
1121 993 910 685 498 191 170
SfiI GGCCn_nnn'nGGCC
Cuts at: 1124 1124
Size: 4568
SfoI GGC'GCC
Cuts at: 758 1352 1379 2000 2248 758
Size: 594 27 621 248 3078
Fragments arranged by size:
3078 621 594 248 27
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SgrAI Cr'CCGG_yG
Cuts at: 524 838 524
Size: 314 4254
SmaI CCC'GGG
Cuts at: 337 422 549 661 740 1079 1478 1590 337
Size: 85 127 112 79 339 399 112 3315
Fragments arranged by size:
3315 399 339 127 112 112 85 79
SmlI C'TyrA_G
Cuts at: 1157 1723 2638 3130 3392 1157
Size: 566 915 492 262 2333
Fragments arranged by size:
2333 915 566 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4068
StuI AGG'CCT
Cuts at: 182 1492 182
Size: 1310 3258
StyI C'CwwG_G
Cuts at: 1176 1702 2056 1176
Size: 526 354 3688
Fragments arranged by size:
3688 526 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1209 2151 2284 2743 2952 1209
Size: 942 133 459 209 2825
Fragments arranged by size:
2825 942 459 209 133
TatI w'GTAC_w
Cuts at: 4083 4083
Size: 4568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4085 4117 4085
Size: 32 4536
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4568
XbaI T'CTAG_A
Cuts at: 1599 1599
Size: 4568
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 2063 441
Size: 1622 2946
XmaI C'CCGG_G
Cuts at: 335 420 547 659 738 1077 1476 1588 335
Size: 85 127 112 79 339 399 112 3315
Fragments arranged by size:
3315 399 339 127 112 112 85 79
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsaXI BsiWI BsrGI BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PsiI PspXI PsrI SexAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI