Restriction map for
pF1KE3637
(Circular) (Six-base) MAPSORT of: pF1KE3637.seq Check: 5226 from: 1 to: 4667
pF1KE3637 4667 bp
With 182 enzymes: SgfI *
August 9, 2016 17:38 ..
AccI GT'mk_AC
Cuts at: 1705 1705
Size: 4667
Acc65I G'GTAC_C
Cuts at: 1683 1683
Size: 4667
AclI AA'CG_TT
Cuts at: 4507 4507
Size: 4667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1608 2492 2924 3671 3939 1608
Size: 884 432 747 268 2336
Fragments arranged by size:
2336 884 747 432 268
AfeI AGC'GCT
Cuts at: 286 994 286
Size: 708 3959
AflIII A'CryG_T
Cuts at: 2019 3123 4100 2019
Size: 1104 977 2586
Fragments arranged by size:
2586 1104 977
AgeI A'CCGG_T
Cuts at: 3055 3055
Size: 4667
AhdI GACnn_n'nnGTC
Cuts at: 219 219
Size: 4667
AlwNI CAG_nnn'CTG
Cuts at: 652 1972 3539 652
Size: 1320 1567 1780
Fragments arranged by size:
1780 1567 1320
ApaI G_GGCC'C
Cuts at: 120 531 659 850 120
Size: 411 128 191 3937
Fragments arranged by size:
3937 411 191 128
ApaLI G'TGCA_C
Cuts at: 3437 3437
Size: 4667
ApoI r'AATT_y
Cuts at: 1671 4020 4276 1671
Size: 2349 256 2062
Fragments arranged by size:
2349 2062 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4667
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
AvaI C'yCGr_G
Cuts at: 398 1010 1687 398
Size: 612 677 3378
Fragments arranged by size:
3378 677 612
BamHI G'GATC_C
Cuts at: 227 1692 227
Size: 1465 3202
BanI G'GyrC_C
Cuts at: 891 1683 2097 2345 2380 891
Size: 792 414 248 35 3178
Fragments arranged by size:
3178 792 414 248 35
BanII G_rGCy'C
Cuts at: 120 531 566 592 659 692 850 1139 1295
Size: 411 35 26 67 33 158 289 156
Cuts at: 1295 1406 1622 1681 2711 120
Size: 111 216 59 1030 2076
Fragments arranged by size:
2076 1030 411 289 216 158 156 111
67 59 35 33 26
BbeI G_GCGC'C
Cuts at: 2101 2349 2101
Size: 248 4419
BbsI GAAGACnn'nnnn_
Cuts at: 172 172
Size: 4667
BbvCI CC'TCA_GC
Cuts at: 740 740
Size: 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 335 369 335
Size: 34 4633
BciVI GTATCCnnnnn_n'
Cuts at: 2565 3326 4667 2565
Size: 761 1341 2565
Fragments arranged by size:
2565 1341 761
BclI T'GATC_A
Cuts at: 2187 2187
Size: 4667
BglII A'GATC_T
Cuts at: 1559 2182 4219 1559
Size: 623 2037 2007
Fragments arranged by size:
2037 2007 623
BlpI GC'TnA_GC
Cuts at: 1779 1779
Size: 4667
Bme1580I G_kGCm'C
Cuts at: 120 531 659 850 1071 2292 2385 3441 120
Size: 411 128 191 221 1221 93 1056 1346
Fragments arranged by size:
1346 1221 1056 411 221 191 128 93
BmrI ACTGGGnnnn_n'
Cuts at: 847 863 1879 1960 2295 4439 847
Size: 16 1016 81 335 2144 1075
Fragments arranged by size:
2144 1075 1016 335 81 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 4667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 994 1465 503
Size: 491 471 3705
Fragments arranged by size:
3705 491 471
Bpu10I CC'TnA_GC
Cuts at: 725 740 725
Size: 15 4652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1843 2722 3214 3512 1843
Size: 879 492 298 2998
Fragments arranged by size:
2998 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4371 4371
Size: 4667
BsaAI yAC'GTr
Cuts at: 1098 2020 1098
Size: 922 3745
BsaBI GATnn'nnATC
Cuts at: 6 2181 4218 6
Size: 2175 2037 455
Fragments arranged by size:
2175 2037 455
BsaHI Gr'CG_yC
Cuts at: 2098 2346 3048 4544 2098
Size: 248 702 1496 2221
Fragments arranged by size:
2221 1496 702 248
BsaWI w'CCGG_w
Cuts at: 885 1856 2377 3055 3329 3476 3887 885
Size: 971 521 678 274 147 411 1665
Fragments arranged by size:
1665 971 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 991 1021 1518 1548 991
Size: 30 497 30 4110
Fragments arranged by size:
4110 497 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 582 582
Size: 4667
BseYI C'CCAG_C
Cuts at: 387 2092 2850 3427 3923 387
Size: 1705 758 577 496 1131
Fragments arranged by size:
1705 1131 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 481 949 291
Size: 190 468 4009
Fragments arranged by size:
4009 468 190
BsiEI CG_ry'CG
Cuts at: 104 336 420 2209 2255 3463 104
Size: 232 84 1789 46 1208 1308
Fragments arranged by size:
1789 1308 1208 232 84 46
BsiHKAI G_wGCw'C
Cuts at: 923 1139 1295 1622 1681 2459 2649 3441 923
Size: 216 156 327 59 778 190 792 2149
Fragments arranged by size:
2149 792 778 327 216 190 156 59
Bsp1286I G_dGCh'C
Cuts at: 120 531 566 592 659 692 850 923 1071
Size: 411 35 26 67 33 158 73 148
Cuts at: 1071 1139 1295 1406 1622 1681 2292 2385 2459
Size: 68 156 111 216 59 611 93 74
Cuts at: 2459 2649 2711 3441 120
Size: 190 62 730 1346
Fragments arranged by size:
1346 730 611 411 216 190 158 156
148 111 93 74 73 68 67 62
59 35 33 26
BspEI T'CCGG_A
Cuts at: 3887 3887
Size: 4667
BspHI T'CATG_A
Cuts at: 3843 3843
Size: 4667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 611 989 1717 1931 2233 2614 611
Size: 378 728 214 302 381 2664
Fragments arranged by size:
2664 728 381 378 302 214
BsrBI CCG'CTC
Cuts at: 50 729 2959 3013 4497 4662 50
Size: 679 2230 54 1484 165 55
Fragments arranged by size:
2230 1484 679 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1184 2579 1184
Size: 1395 3272
BsrFI r'CCGG_y
Cuts at: 2665 2846 3055 2665
Size: 181 209 4277
Fragments arranged by size:
4277 209 181
BssSI C'ACGA_G
Cuts at: 2938 3296 2938
Size: 358 4309
BstAPI GCAn_nnn'nTGC
Cuts at: 898 927 2044 898
Size: 29 1117 3521
Fragments arranged by size:
3521 1117 29
BstBI TT'CG_AA
Cuts at: 3028 3028
Size: 4667
BstXI CCAn_nnnn'nTGG
Cuts at: 675 675
Size: 4667
BstYI r'GATC_y
Cuts at: 1 227 1559 1692 2159 2182 2517 2763 3775
Size: 226 1332 133 467 23 335 246 1012
Cuts at: 3775 3861 3873 4219 1
Size: 86 12 346 449
Fragments arranged by size:
1332 1012 467 449 346 335 246 226
133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 608 645 608
Size: 37 4630
BtgI C'CryG_G
Cuts at: 107 107
Size: 4667
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1465 2617 2798 2961 1465
Size: 1152 181 163 3171
Fragments arranged by size:
3171 1152 181 163
BtsI GCAGTG_nn'
Cuts at: 360 1910 360
Size: 1550 3117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 189 224 1166 1201 1236 1271 189
Size: 35 942 35 35 35 3585
Fragments arranged by size:
3585 942 35 35 35 35
DraI TTT'AAA
Cuts at: 1666 1666
Size: 4667
DraIII CAC_nnn'GTG
Cuts at: 927 927
Size: 4667
DrdI GACnn_nn'nnGTC
Cuts at: 2373 3231 2373
Size: 858 3809
EaeI y'GGCC_r
Cuts at: 417 677 1396 2252 2426 2817 2844 4189 417
Size: 260 719 856 174 391 27 1345 895
Fragments arranged by size:
1345 895 856 719 391 260 174 27
EagI C'GGCC_G
Cuts at: 417 2252 417
Size: 1835 2832
EarI CTCTTCn'nnn_
Cuts at: 1627 2690 2900 1627
Size: 1063 210 3394
Fragments arranged by size:
3394 1063 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 2875 3185 3331 4477 262
Size: 2613 310 146 1146 452
Fragments arranged by size:
2613 1146 452 310 146
EcoICRI GAG'CTC
Cuts at: 1137 1293 1620 1679 1137
Size: 156 327 59 4125
Fragments arranged by size:
4125 327 156 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 994 1465 1608 2492 2924 3671 3939 503
Size: 491 471 143 884 432 747 268 1231
Fragments arranged by size:
1231 884 747 491 471 432 268 143
EcoNI CCTnn'n_nnAGG
Cuts at: 521 607 1081 521
Size: 86 474 4107
Fragments arranged by size:
4107 474 86
EcoO109I rG'GnC_Cy
Cuts at: 116 117 527 656 698 846 978 1806 116
Size: 1 410 129 42 148 132 828 2977
Fragments arranged by size:
2977 828 410 148 132 129 42 1
EcoRI G'AATT_C
Cuts at: 1671 1671
Size: 4667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 285 1638 2686 285
Size: 1353 1048 2266
Fragments arranged by size:
2266 1353 1048
FspI TGC'GCA
Cuts at: 2448 2448
Size: 4667
HaeII r_GCGC'y
Cuts at: 288 388 996 2101 2349 3371 4350 288
Size: 100 608 1105 248 1022 979 605
Fragments arranged by size:
1105 1022 979 608 605 248 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 392 424 2509 2539 2541 2571 205
Size: 32 155 32 2085 30 2 30 2301
Fragments arranged by size:
2301 2085 155 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1706 1706
Size: 4667
HindIII A'AGCT_T
Cuts at: 1519 1519
Size: 4667
KasI G'GCGC_C
Cuts at: 2097 2345 2097
Size: 248 4419
KpnI G_GTAC'C
Cuts at: 1687 1687
Size: 4667
MluI A'CGCG_T
Cuts at: 4100 4100
Size: 4667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 692 1022 3338 3522 692
Size: 330 2316 184 1837
Fragments arranged by size:
2316 1837 330 184
MscI TGG'CCA
Cuts at: 679 2428 679
Size: 1749 2918
MslI CAynn'nnrTG
Cuts at: 2656 2783 2656
Size: 127 4540
MspA1I CmG'CkG
Cuts at: 471 480 579 777 966 1431 1778 2092 2452
Size: 9 99 198 189 465 347 314 360
Cuts at: 2452 3465 3710 471
Size: 1013 245 1428
Fragments arranged by size:
1428 1013 465 360 347 314 245 198
189 99 9
NaeI GCC'GGC
Cuts at: 2848 2848
Size: 4667
NarI GG'CG_CC
Cuts at: 2098 2346 2098
Size: 248 4419
NcoI C'CATG_G
Cuts at: 107 107
Size: 4667
NgoMIV G'CCGG_C
Cuts at: 2846 2846
Size: 4667
NspI r_CATG'y
Cuts at: 396 426 454 962 1546 1720 2220 3127 396
Size: 30 28 508 584 174 500 907 1936
Fragments arranged by size:
1936 907 584 508 500 174 30 28
PasI CC'CwG_GG
Cuts at: 585 585
Size: 4667
PciI A'CATG_T
Cuts at: 3123 3123
Size: 4667
PflMI CCAn_nnn'nTGG
Cuts at: 1545 1545
Size: 4667
PmeI GTTT'AAAC
Cuts at: 1666 1666
Size: 4667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4635
PpuMI rG'GwC_Cy
Cuts at: 978 978
Size: 4667
PspOMI G'GGCC_C
Cuts at: 116 527 655 846 116
Size: 411 128 191 3937
Fragments arranged by size:
3937 411 191 128
PstI C_TGCA'G
Cuts at: 374 476 1288 1436 1559 1714 2399 374
Size: 102 812 148 123 155 685 2642
Fragments arranged by size:
2642 812 685 155 148 123 102
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4667
PvuII CAG'CTG
Cuts at: 471 579 777 966 1431 2092 2452 471
Size: 108 198 189 465 661 360 2686
Fragments arranged by size:
2686 661 465 360 198 189 108
RsrII CG'GwC_CG
Cuts at: 2862 2862
Size: 4667
SacI G_AGCT'C
Cuts at: 1139 1295 1622 1681 1139
Size: 156 327 59 4125
Fragments arranged by size:
4125 327 156 59
SalI G'TCGA_C
Cuts at: 1704 1704
Size: 4667
SapI GCTCTTCn'nnn_
Cuts at: 1627 2690 2900 1627
Size: 1063 210 3394
Fragments arranged by size:
3394 1063 210
SbfI CC_TGCA'GG
Cuts at: 1714 1714
Size: 4667
ScaI AGT'ACT
Cuts at: 4184 4184
Size: 4667
SexAI A'CCwGG_T
Cuts at: 923 923
Size: 4667
SfcI C'TryA_G
Cuts at: 33 370 472 759 948 1284 1432 1555 1710
Size: 337 102 287 189 336 148 123 155
Cuts at: 1710 2395 3388 3579 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 337 336 287 191 189
155 148 123 102
SfoI GGC'GCC
Cuts at: 2099 2347 2099
Size: 248 4419
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
SmaI CCC'GGG
Cuts at: 1012 1689 1012
Size: 677 3990
SmlI C'TyrA_G
Cuts at: 1822 2737 3229 3491 1822
Size: 915 492 262 2998
Fragments arranged by size:
2998 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4667
SphI G_CATG'C
Cuts at: 454 1720 2220 454
Size: 1266 500 2901
Fragments arranged by size:
2901 1266 500
StyI C'CwwG_G
Cuts at: 107 531 557 680 1277 1511 1658 1801 2155
Size: 424 26 123 597 234 147 143 354
Cuts at: 2155 107
Size: 2619
Fragments arranged by size:
2619 597 424 354 234 147 143 123
26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 803 1524 2250 2383 2842 3051 803
Size: 721 726 133 459 209 2419
Fragments arranged by size:
2419 726 721 459 209 133
TatI w'GTAC_w
Cuts at: 1436 4182 1436
Size: 2746 1921
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4184 4216 4184
Size: 32 4635
Tth111I GACn'n_nGTC
Cuts at: 2464 2464
Size: 4667
XbaI T'CTAG_A
Cuts at: 1698 1698
Size: 4667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1095 2162 1095
Size: 1067 3600
XmaI C'CCGG_G
Cuts at: 1010 1687 1010
Size: 677 3990
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI