Restriction map for
pF1KE3557
(Circular) (Six-base) MAPSORT of: pF1KE3557.seq Check: 2023 from: 1 to: 4757
pF1KE3557 4757 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AatII G_ACGT'C
Cuts at: 1376 1376
Size: 4757
AccI GT'mk_AC
Cuts at: 1795 1795
Size: 4757
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2582 3014 3761 4029 1417
Size: 1165 432 747 268 2145
Fragments arranged by size:
2145 1165 747 432 268
AfeI AGC'GCT
Cuts at: 988 988
Size: 4757
AflIII A'CryG_T
Cuts at: 2109 3213 4190 2109
Size: 1104 977 2676
Fragments arranged by size:
2676 1104 977
AgeI A'CCGG_T
Cuts at: 3145 3145
Size: 4757
AhdI GACnn_n'nnGTC
Cuts at: 975 975
Size: 4757
AleI CACnn'nnGTG
Cuts at: 644 644
Size: 4757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 662 1627 662
Size: 965 3792
AlwNI CAG_nnn'CTG
Cuts at: 556 890 924 1134 1499 2062 3629 556
Size: 334 34 210 365 563 1567 1684
Fragments arranged by size:
1684 1567 563 365 334 210 34
ApaI G_GGCC'C
Cuts at: 792 1054 1640 792
Size: 262 586 3909
Fragments arranged by size:
3909 586 262
ApaLI G'TGCA_C
Cuts at: 3527 3527
Size: 4757
ApoI r'AATT_y
Cuts at: 1761 4110 4366 1761
Size: 2349 256 2152
Fragments arranged by size:
2349 2152 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 160 175 196 1420 1654 1777 160
Size: 15 21 1224 234 123 3140
Fragments arranged by size:
3140 1224 234 123 21 15
BamHI G'GATC_C
Cuts at: 1782 1782
Size: 4757
BanI G'GyrC_C
Cuts at: 1212 1773 2187 2435 2470 1212
Size: 561 414 248 35 3499
Fragments arranged by size:
3499 561 414 248 35
BanII G_rGCy'C
Cuts at: 127 223 236 792 1054 1395 1427 1640 1715
Size: 96 13 556 262 341 32 213 75
Cuts at: 1715 1771 2801 127
Size: 56 1030 2083
Fragments arranged by size:
2083 1030 556 341 262 213 96 75
56 32 13
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BbvCI CC'TCA_GC
Cuts at: 766 766
Size: 4757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4723
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3416 4757 2655
Size: 761 1341 2655
Fragments arranged by size:
2655 1341 761
BclI T'GATC_A
Cuts at: 2277 2277
Size: 4757
BglI GCCn_nnn'nGGC
Cuts at: 842 1058 1249 842
Size: 216 191 4350
Fragments arranged by size:
4350 216 191
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 792 1054 1640 2382 2475 3531 792
Size: 262 586 742 93 1056 2018
Fragments arranged by size:
2018 1056 742 586 262 93
BmrI ACTGGGnnnn_n'
Cuts at: 1969 2050 2385 4529 1969
Size: 81 335 2144 2197
Fragments arranged by size:
2197 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 405 555 3124 405
Size: 150 2569 2038
Fragments arranged by size:
2569 2038 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 470 663 685 844 1084 1093 364
Size: 106 193 22 159 240 9 4028
Fragments arranged by size:
4028 240 193 159 106 22 9
Bpu10I CC'TnA_GC
Cuts at: 121 766 908 121
Size: 645 142 3970
Fragments arranged by size:
3970 645 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1933 2812 3304 3602 1095
Size: 838 879 492 298 2250
Fragments arranged by size:
2250 879 838 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1660 4461 1660
Size: 2801 1956
BsaAI yAC'GTr
Cuts at: 2110 2110
Size: 4757
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 674 1373 2188 2436 3138 4634 674
Size: 699 815 248 702 1496 797
Fragments arranged by size:
1496 815 797 702 699 248
BsaWI w'CCGG_w
Cuts at: 629 1387 1946 2467 3145 3419 3566 3977 629
Size: 758 559 521 678 274 147 411 1409
Fragments arranged by size:
1409 758 678 559 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 378 505 961 1027 1218 214
Size: 164 127 456 66 191 3753
Fragments arranged by size:
3753 456 191 164 127 66
BseYI C'CCAG_C
Cuts at: 468 501 527 1364 2182 2940 3517 4013 468
Size: 33 26 837 818 758 577 496 1212
Fragments arranged by size:
1212 837 818 758 577 496 33 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 1633 1246
Size: 387 4370
BsiEI CG_ry'CG
Cuts at: 104 1027 2299 2345 3553 104
Size: 923 1272 46 1208 1308
Fragments arranged by size:
1308 1272 1208 923 46
BsiHKAI G_wGCw'C
Cuts at: 593 1589 1715 1771 2549 2739 3531 593
Size: 996 126 56 778 190 792 1819
Fragments arranged by size:
1819 996 792 778 190 126 56
BsmI GAATG_Cn'
Cuts at: 1035 1035
Size: 4757
BsmBI CGTCTCn'nnnn_
Cuts at: 157 157
Size: 4757
Bsp1286I G_dGCh'C
Cuts at: 127 223 236 593 792 1054 1395 1427 1589
Size: 96 13 357 199 262 341 32 162
Cuts at: 1589 1640 1715 1771 2382 2475 2549 2739 2801
Size: 51 75 56 611 93 74 190 62
Cuts at: 2801 3531 127
Size: 730 1353
Fragments arranged by size:
1353 730 611 357 341 262 199 190
162 96 93 75 74 62 56 51
32 13
BspEI T'CCGG_A
Cuts at: 629 3977 629
Size: 3348 1409
BspHI T'CATG_A
Cuts at: 3933 3933
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1807 2021 2323 2704 1807
Size: 214 302 381 3860
Fragments arranged by size:
3860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1531 3049 3103 4587 4752 50
Size: 1481 1518 54 1484 165 55
Fragments arranged by size:
1518 1484 1481 165 55 54
BsrDI GCAATG_nn'
Cuts at: 902 1457 2669 902
Size: 555 1212 2990
Fragments arranged by size:
2990 1212 555
BsrFI r'CCGG_y
Cuts at: 2755 2936 3145 2755
Size: 181 209 4367
Fragments arranged by size:
4367 209 181
BssHII G'CGCG_C
Cuts at: 1175 1175
Size: 4757
BssSI C'ACGA_G
Cuts at: 3028 3386 3028
Size: 358 4399
BstAPI GCAn_nnn'nTGC
Cuts at: 1134 2134 1134
Size: 1000 3757
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstXI CCAn_nnnn'nTGG
Cuts at: 573 1686 573
Size: 1113 3644
BstYI r'GATC_y
Cuts at: 1 1782 2249 2272 2607 2853 3865 3951 3963
Size: 1781 467 23 335 246 1012 86 12
Cuts at: 3963 4309 1
Size: 346 449
Fragments arranged by size:
1781 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 721 721
Size: 4757
BtgI C'CryG_G
Cuts at: 145 215 354 1055 1254 1685 145
Size: 70 139 701 199 431 3217
Fragments arranged by size:
3217 701 431 199 139 70
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 580 2707 2888 3051 580
Size: 2127 181 163 2286
Fragments arranged by size:
2286 2127 181 163
BtsI GCAGTG_nn'
Cuts at: 337 1123 1237 1732 2000 337
Size: 786 114 495 268 3094
Fragments arranged by size:
3094 786 495 268 114
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 2463 3321 2463
Size: 858 3899
EaeI y'GGCC_r
Cuts at: 1181 1298 1463 1622 2342 2516 2907 2934 4279
Size: 117 165 159 720 174 391 27 1345
Cuts at: 4279 1181
Size: 1659
Fragments arranged by size:
1659 1345 720 391 174 165 159 117
27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 1408 1435 1564 1658 2780 2990 1408
Size: 27 129 94 1122 210 3175
Fragments arranged by size:
3175 1122 210 129 94 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1438 2965 3275 3421 4567 1438
Size: 1527 310 146 1146 1628
Fragments arranged by size:
1628 1527 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1713 1769 1713
Size: 56 4701
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 470 663 685 844 1084 1093 1417 2582
Size: 106 193 22 159 240 9 324 1165
Cuts at: 2582 3014 3761 4029 364
Size: 432 747 268 1092
Fragments arranged by size:
1165 1092 747 432 324 268 240 193
159 106 22 9
EcoO109I rG'GnC_Cy
Cuts at: 141 348 414 498 789 1051 1896 141
Size: 207 66 84 291 262 845 3002
Fragments arranged by size:
3002 845 291 262 207 84 66
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 384 1422 2776 384
Size: 1038 1354 2365
Fragments arranged by size:
2365 1354 1038
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 990 1344 1365 2191 2439 3461 4440 990
Size: 354 21 826 248 1022 979 1307
Fragments arranged by size:
1307 1022 979 826 354 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 961 993 2599 2629 2631 2661 961
Size: 32 1606 30 2 30 3057
Fragments arranged by size:
3057 1606 32 30 30 2
HincII GTy'rAC
Cuts at: 1796 1796
Size: 4757
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3428 3612 3428
Size: 184 4573
MscI TGG'CCA
Cuts at: 1300 1624 2518 1300
Size: 324 894 3539
Fragments arranged by size:
3539 894 324
MslI CAynn'nnrTG
Cuts at: 644 1609 1678 1684 2746 2873 644
Size: 965 69 6 1062 127 2528
Fragments arranged by size:
2528 1062 965 127 69 6
MspA1I CmG'CkG
Cuts at: 147 553 1256 1499 1868 2182 2542 3555 3800
Size: 406 703 243 369 314 360 1013 245
Cuts at: 3800 147
Size: 1104
Fragments arranged by size:
1104 1013 703 406 369 360 314 245
243
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 1685 1685
Size: 4757
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NspI r_CATG'y
Cuts at: 1608 1677 1810 2310 3217 1608
Size: 69 133 500 907 3148
Fragments arranged by size:
3148 907 500 133 69
PasI CC'CwG_GG
Cuts at: 136 1047 136
Size: 911 3846
PciI A'CATG_T
Cuts at: 3213 3213
Size: 4757
PflMI CCAn_nnn'nTGG
Cuts at: 1707 1707
Size: 4757
PfoI T'CCnGG_A
Cuts at: 652 917 652
Size: 265 4492
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4725
PpuMI rG'GwC_Cy
Cuts at: 141 348 414 498 141
Size: 207 66 84 4400
Fragments arranged by size:
4400 207 84 66
PspOMI G'GGCC_C
Cuts at: 788 1050 1636 788
Size: 262 586 3909
Fragments arranged by size:
3909 586 262
PstI C_TGCA'G
Cuts at: 714 1130 1724 1804 2489 714
Size: 416 594 80 685 2982
Fragments arranged by size:
2982 685 594 416 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 553 2182 2542 553
Size: 1629 360 2768
Fragments arranged by size:
2768 1629 360
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1715 1771 1715
Size: 56 4701
SacII CC_GC'GG
Cuts at: 148 1257 148
Size: 1109 3648
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SanDI GG'GwC_CC
Cuts at: 141 141
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 1408 1564 2780 2990 1408
Size: 156 1216 210 3175
Fragments arranged by size:
3175 1216 210 156
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SexAI A'CCwGG_T
Cuts at: 1160 1700 1160
Size: 540 4217
SfcI C'TryA_G
Cuts at: 33 710 1126 1720 1800 2485 3478 3669 33
Size: 677 416 594 80 685 993 191 1121
Fragments arranged by size:
1121 993 685 677 594 416 191 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 1058 1058
Size: 4757
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 1779 1779
Size: 4757
SmlI C'TyrA_G
Cuts at: 1074 1912 2827 3319 3581 1074
Size: 838 915 492 262 2250
Fragments arranged by size:
2250 915 838 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1677 1810 2310 1677
Size: 133 500 4124
Fragments arranged by size:
4124 500 133
StyI C'CwwG_G
Cuts at: 572 1685 1891 2245 572
Size: 1113 206 354 3084
Fragments arranged by size:
3084 1113 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 324 1041 2340 2473 2932 3141 324
Size: 717 1299 133 459 209 1940
Fragments arranged by size:
1940 1299 717 459 209 133
TatI w'GTAC_w
Cuts at: 752 4272 752
Size: 3520 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4274 4306 4274
Size: 32 4725
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2252
Size: 4757
XmaI C'CCGG_G
Cuts at: 1777 1777
Size: 4757
XmnI GAAnn'nnTTC
Cuts at: 1402 1402
Size: 4757
ZraI GAC'GTC
Cuts at: 1374 1374
Size: 4757
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AflII AloI AscI AvrII BaeI BbsI BfrBI
BmgBI BmtI BsaXI BsiWI BsrGI BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI