Restriction map for
pF1KE3552
(Circular) (Six-base) MAPSORT of: pF1KE3552.seq Check: 7456 from: 1 to: 8987
pF1KE3552 8987 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 1425 1555 1425
Size: 130 8857
AccI GT'mk_AC
Cuts at: 4178 4914 6025 4178
Size: 736 1111 7140
Fragments arranged by size:
7140 1111 736
Acc65I G'GTAC_C
Cuts at: 6003 6003
Size: 8987
AclI AA'CG_TT
Cuts at: 8827 8827
Size: 8987
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 1022 2198 2633 2836 2877 3194 3771 3970
Size: 780 1176 435 203 41 317 577 199
Cuts at: 3970 4160 4628 5039 5135 5166 6812 7244 7991
Size: 190 468 411 96 31 1646 432 747
Cuts at: 7991 8259 242
Size: 268 970
Fragments arranged by size:
1646 1176 970 780 747 577 468 435
432 411 317 268 203 199 190 96
41 31
AflII C'TTAA_G
Cuts at: 1144 3928 1144
Size: 2784 6203
AflIII A'CryG_T
Cuts at: 118 5444 6339 7443 8420 118
Size: 5326 895 1104 977 685
Fragments arranged by size:
5326 1104 977 895 685
AgeI A'CCGG_T
Cuts at: 5615 7375 5615
Size: 1760 7227
AhdI GACnn_n'nnGTC
Cuts at: 2862 2935 2862
Size: 73 8914
AleI CACnn'nnGTG
Cuts at: 895 895
Size: 8987
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1426 4345 5549 1426
Size: 2919 1204 4864
Fragments arranged by size:
4864 2919 1204
AlwNI CAG_nnn'CTG
Cuts at: 328 4738 6292 7859 328
Size: 4410 1554 1567 1456
Fragments arranged by size:
4410 1567 1554 1456
ApaLI G'TGCA_C
Cuts at: 7757 7757
Size: 8987
ApoI r'AATT_y
Cuts at: 1136 1242 1751 1862 1899 3966 5109 5556 5991
Size: 106 509 111 37 2067 1143 447 435
Cuts at: 5991 8340 8596 1136
Size: 2349 256 1527
Fragments arranged by size:
2349 2067 1527 1143 509 447 435 256
111 106 37
AseI AT'TA_AT
Cuts at: 20 2659 3595 20
Size: 2639 936 5412
Fragments arranged by size:
5412 2639 936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8987
AvaI C'yCGr_G
Cuts at: 1931 2481 6007 1931
Size: 550 3526 4911
Fragments arranged by size:
4911 3526 550
AvrII C'CTAG_G
Cuts at: 1738 1738
Size: 8987
BamHI G'GATC_C
Cuts at: 6012 6012
Size: 8987
BanI G'GyrC_C
Cuts at: 6003 6417 6665 6700 6003
Size: 414 248 35 8290
Fragments arranged by size:
8290 414 248 35
BanII G_rGCy'C
Cuts at: 4690 4924 5433 6001 7031 4690
Size: 234 509 568 1030 6646
Fragments arranged by size:
6646 1030 568 509 234
BbeI G_GCGC'C
Cuts at: 6421 6669 6421
Size: 248 8739
BbsI GAAGACnn'nnnn_
Cuts at: 593 2861 2883 3182 3958 4046 4757 593
Size: 2268 22 299 776 88 711 4823
Fragments arranged by size:
4823 2268 776 711 299 88 22
BciVI GTATCCnnnnn_n'
Cuts at: 399 6885 7646 8987 399
Size: 6486 761 1341 399
Fragments arranged by size:
6486 1341 761 399
BclI T'GATC_A
Cuts at: 2553 6507 2553
Size: 3954 5033
BglI GCCn_nnn'nGGC
Cuts at: 112 2361 4780 112
Size: 2249 2419 4319
Fragments arranged by size:
4319 2419 2249
BglII A'GATC_T
Cuts at: 1192 1872 6502 8539 1192
Size: 680 4630 2037 1640
Fragments arranged by size:
4630 2037 1640 680
BlpI GC'TnA_GC
Cuts at: 2717 4756 5634 6099 2717
Size: 2039 878 465 5605
Fragments arranged by size:
5605 2039 878 465
Bme1580I G_kGCm'C
Cuts at: 1451 2124 5528 6612 6705 7761 1451
Size: 673 3404 1084 93 1056 2677
Fragments arranged by size:
3404 2677 1084 1056 673 93
BmgBI CAC'GTC
Cuts at: 2440 3146 4763 2440
Size: 706 1617 6664
Fragments arranged by size:
6664 1617 706
BmrI ACTGGGnnnn_n'
Cuts at: 1446 2153 4510 5345 6199 6280 6615 8759 1446
Size: 707 2357 835 854 81 335 2144 1674
Fragments arranged by size:
2357 2144 1674 854 835 707 335 81
BmtI G_CTAG'C
Cuts at: 3196 3196
Size: 8987
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1050 1230 4752 7354 1050
Size: 180 3522 2602 2683
Fragments arranged by size:
3522 2683 2602 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1098 2186 3266 4893 1098
Size: 1088 1080 1627 5192
Fragments arranged by size:
5192 1627 1088 1080
Bpu10I CC'TnA_GC
Cuts at: 3800 5701 3800
Size: 1901 7086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 263 626 1167 1181 1523 3669 6163 7042
Size: 121 363 541 14 342 2146 2494 879
Cuts at: 7042 7534 7832 142
Size: 492 298 1297
Fragments arranged by size:
2494 2146 1297 879 541 492 363 342
298 121 14
BsaI GGTCTCn'nnnn_
Cuts at: 177 2392 3106 5415 8691 177
Size: 2215 714 2309 3276 473
Fragments arranged by size:
3276 2309 2215 714 473
BsaAI yAC'GTr
Cuts at: 2805 6340 2805
Size: 3535 5452
BsaBI GATnn'nnATC
Cuts at: 6 2745 3097 6501 8538 6
Size: 2739 352 3404 2037 455
Fragments arranged by size:
3404 2739 2037 455 352
BsaHI Gr'CG_yC
Cuts at: 1422 1552 2340 3545 6418 6666 7368 8864 1422
Size: 130 788 1205 2873 248 702 1496 1545
Fragments arranged by size:
2873 1545 1496 1205 788 702 248 130
BsaWI w'CCGG_w
Cuts at: 5615 6176 6697 7375 7649 7796 8207 5615
Size: 561 521 678 274 147 411 6395
Fragments arranged by size:
6395 678 561 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 485 515 1773 1803 2389 2419 2616 2637 2646
Size: 30 1258 30 586 30 197 21 9
Cuts at: 2646 2667 485
Size: 21 6805
Fragments arranged by size:
6805 1258 586 197 30 30 30 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 386 761 1847 4489 5723 386
Size: 375 1086 2642 1234 3650
Fragments arranged by size:
3650 2642 1234 1086 375
BseYI C'CCAG_C
Cuts at: 4184 4555 6412 7170 7747 8243 4184
Size: 371 1857 758 577 496 4928
Fragments arranged by size:
4928 1857 758 577 496 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4180 4180
Size: 8987
BsiEI CG_ry'CG
Cuts at: 104 5765 6529 6575 7783 104
Size: 5661 764 46 1208 1308
Fragments arranged by size:
5661 1308 1208 764 46
BsiHKAI G_wGCw'C
Cuts at: 1548 4600 6001 6779 6969 7761 1548
Size: 3052 1401 778 190 792 2774
Fragments arranged by size:
3052 2774 1401 792 778 190
BsmI GAATG_Cn'
Cuts at: 3163 3485 5478 5925 3163
Size: 322 1993 447 6225
Fragments arranged by size:
6225 1993 447 322
BsmBI CGTCTCn'nnnn_
Cuts at: 1559 1559
Size: 8987
Bsp1286I G_dGCh'C
Cuts at: 1451 1548 2124 4600 4690 4924 5433 5528 6001
Size: 97 576 2476 90 234 509 95 473
Cuts at: 6001 6612 6705 6779 6969 7031 7761 1451
Size: 611 93 74 190 62 730 2677
Fragments arranged by size:
2677 2476 730 611 576 509 473 234
190 97 95 93 90 74 62
BspEI T'CCGG_A
Cuts at: 8207 8207
Size: 8987
BspHI T'CATG_A
Cuts at: 1437 2556 3497 8163 1437
Size: 1119 941 4666 2261
Fragments arranged by size:
4666 2261 1119 941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 450 2425 6037 6251 6553 6934 450
Size: 1975 3612 214 302 381 2503
Fragments arranged by size:
3612 2503 1975 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5582 7279 7333 8817 8982 50
Size: 5532 1697 54 1484 165 55
Fragments arranged by size:
5532 1697 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 687 915 1882 4523 5784 6899 687
Size: 228 967 2641 1261 1115 2775
Fragments arranged by size:
2775 2641 1261 1115 967 228
BsrFI r'CCGG_y
Cuts at: 5615 6985 7166 7375 5615
Size: 1370 181 209 7227
Fragments arranged by size:
7227 1370 209 181
BsrGI T'GTAC_A
Cuts at: 463 3023 463
Size: 2560 6427
BssSI C'ACGA_G
Cuts at: 5747 7258 7616 5747
Size: 1511 358 7118
Fragments arranged by size:
7118 1511 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3360 5920 6364 3360
Size: 2560 444 5983
Fragments arranged by size:
5983 2560 444
BstBI TT'CG_AA
Cuts at: 4029 7348 4029
Size: 3319 5668
BstEII G'GTnAC_C
Cuts at: 469 1952 4059 469
Size: 1483 2107 5397
Fragments arranged by size:
5397 2107 1483
BstXI CCAn_nnnn'nTGG
Cuts at: 1122 5814 1122
Size: 4692 4295
BstYI r'GATC_y
Cuts at: 1 1192 1840 1872 3261 3858 4300 5721 6012
Size: 1191 648 32 1389 597 442 1421 291
Cuts at: 6012 6479 6502 6837 7083 8095 8181 8193 8539
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8539 1
Size: 449
Fragments arranged by size:
1421 1389 1191 1012 648 597 467 449
442 346 335 291 246 86 32 23
12
Bsu36I CC'TnA_GG
Cuts at: 2317 4925 2317
Size: 2608 6379
BtgI C'CryG_G
Cuts at: 746 2977 4937 746
Size: 2231 1960 4796
Fragments arranged by size:
4796 2231 1960
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6937 7118 7281 6937
Size: 181 163 8643
Fragments arranged by size:
8643 181 163
BtsI GCAGTG_nn'
Cuts at: 902 1482 1924 6230 902
Size: 580 442 4306 3659
Fragments arranged by size:
4306 3659 580 442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1674 1709 1674
Size: 35 8952
DraI TTT'AAA
Cuts at: 1040 1857 5986 1040
Size: 817 4129 4041
Fragments arranged by size:
4129 4041 817
DraIII CAC_nnn'GTG
Cuts at: 5469 5469
Size: 8987
DrdI GACnn_nn'nnGTC
Cuts at: 132 3051 3247 6693 7551 132
Size: 2919 196 3446 858 1568
Fragments arranged by size:
3446 2919 1568 858 196
EaeI y'GGCC_r
Cuts at: 113 241 2974 4838 5666 6572 6746 7137 7164
Size: 128 2733 1864 828 906 174 391 27
Cuts at: 7164 8509 113
Size: 1345 591
Fragments arranged by size:
2733 1864 1345 906 828 591 391 174
128 27
EagI C'GGCC_G
Cuts at: 6572 6572
Size: 8987
EarI CTCTTCn'nnn_
Cuts at: 3056 3230 3407 3814 4136 4721 5185 5786 7010
Size: 174 177 407 322 585 464 601 1224
Cuts at: 7010 7220 3056
Size: 210 4823
Fragments arranged by size:
4823 1224 601 585 464 407 322 210
177 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2483 7195 7505 7651 8797 2483
Size: 4712 310 146 1146 2673
Fragments arranged by size:
4712 2673 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5999 5999
Size: 8987
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 1022 1098 2186 2198 2633 2836 2877 3194
Size: 780 76 1088 12 435 203 41 317
Cuts at: 3194 3266 3771 3970 4160 4628 4893 5039 5135
Size: 72 505 199 190 468 265 146 96
Cuts at: 5135 5166 6812 7244 7991 8259 242
Size: 31 1646 432 747 268 970
Fragments arranged by size:
1646 1088 970 780 747 505 468 435
432 317 268 265 203 199 190 146
96 76 72 41 31 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3166 3166
Size: 8987
EcoO109I rG'GnC_Cy
Cuts at: 247 439 833 985 2610 5643 5831 6126 247
Size: 192 394 152 1625 3033 188 295 3108
Fragments arranged by size:
3108 3033 1625 394 295 192 188 152
EcoRI G'AATT_C
Cuts at: 5991 5991
Size: 8987
EcoRV GAT'ATC
Cuts at: 4580 4580
Size: 8987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 4165 7006 2935
Size: 1230 2841 4916
Fragments arranged by size:
4916 2841 1230
FspI TGC'GCA
Cuts at: 6768 6768
Size: 8987
HaeII r_GCGC'y
Cuts at: 6421 6669 7691 8670 6421
Size: 248 1022 979 6738
Fragments arranged by size:
6738 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 946 978 1165 1197 2389 2421 2475 2507 2674
Size: 32 187 32 1192 32 54 32 167
Cuts at: 2674 2706 2848 2866 2880 2898 2921 2953 4918
Size: 32 142 18 14 18 23 32 1965
Cuts at: 4918 4950 5578 5610 5699 5731 6829 6859 6861
Size: 32 628 32 89 32 1098 30 2
Cuts at: 6861 6891 946
Size: 30 3042
Fragments arranged by size:
3042 1965 1192 1098 628 187 167 142
89 54 32 32 32 32 32 32
32 32 32 30 30 23 18 18
14 2
HincII GTy'rAC
Cuts at: 3603 4915 5297 5333 6026 3603
Size: 1312 382 36 693 6564
Fragments arranged by size:
6564 1312 693 382 36
HindIII A'AGCT_T
Cuts at: 668 5880 668
Size: 5212 3775
HpaI GTT'AAC
Cuts at: 5297 5297
Size: 8987
KasI G'GCGC_C
Cuts at: 6417 6665 6417
Size: 248 8739
KpnI G_GTAC'C
Cuts at: 6007 6007
Size: 8987
MluI A'CGCG_T
Cuts at: 118 8420 118
Size: 8302 685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 103 1303 2952 3173 3398 3573 7658 7842 103
Size: 1200 1649 221 225 175 4085 184 1248
Fragments arranged by size:
4085 1649 1248 1200 225 221 184 175
MscI TGG'CCA
Cuts at: 115 243 4840 5668 6748 115
Size: 128 4597 828 1080 2354
Fragments arranged by size:
4597 2354 1080 828 128
MslI CAynn'nnrTG
Cuts at: 895 1172 3917 4066 5531 6976 7103 895
Size: 277 2745 149 1465 1445 127 2779
Fragments arranged by size:
2779 2745 1465 1445 277 149 127
MspA1I CmG'CkG
Cuts at: 4354 4661 5608 6098 6412 6772 7785 8030 4354
Size: 307 947 490 314 360 1013 245 5311
Fragments arranged by size:
5311 1013 947 490 360 314 307 245
NaeI GCC'GGC
Cuts at: 7168 7168
Size: 8987
NarI GG'CG_CC
Cuts at: 6418 6666 6418
Size: 248 8739
NcoI C'CATG_G
Cuts at: 746 2977 746
Size: 2231 6756
NdeI CA'TA_TG
Cuts at: 2751 3607 2751
Size: 856 8131
NgoMIV G'CCGG_C
Cuts at: 7166 7166
Size: 8987
NheI G'CTAG_C
Cuts at: 3192 3192
Size: 8987
NspI r_CATG'y
Cuts at: 2005 4071 5448 6040 6540 7447 2005
Size: 2066 1377 592 500 907 3545
Fragments arranged by size:
3545 2066 1377 907 592 500
PciI A'CATG_T
Cuts at: 5444 7443 5444
Size: 1999 6988
PfoI T'CCnGG_A
Cuts at: 3212 3663 5218 3212
Size: 451 1555 6981
Fragments arranged by size:
6981 1555 451
PmeI GTTT'AAAC
Cuts at: 5986 5986
Size: 8987
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8126 8158 8126
Size: 32 8955
PpuMI rG'GwC_Cy
Cuts at: 247 439 985 2610 5643 247
Size: 192 546 1625 3033 3591
Fragments arranged by size:
3591 3033 1625 546 192
PshAI GACnn'nnGTC
Cuts at: 4234 4910 4234
Size: 676 8311
PsiI TTA'TAA
Cuts at: 481 1382 481
Size: 901 8086
PspXI vC'TCGA_Gb
Cuts at: 2481 2481
Size: 8987
PstI C_TGCA'G
Cuts at: 1110 5916 6034 6719 1110
Size: 4806 118 685 3378
Fragments arranged by size:
4806 3378 685 118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8987
PvuII CAG'CTG
Cuts at: 4354 4661 5608 6412 6772 4354
Size: 307 947 804 360 6569
Fragments arranged by size:
6569 947 804 360 307
RsrII CG'GwC_CG
Cuts at: 7182 7182
Size: 8987
SacI G_AGCT'C
Cuts at: 6001 6001
Size: 8987
SalI G'TCGA_C
Cuts at: 4913 6024 4913
Size: 1111 7876
SapI GCTCTTCn'nnn_
Cuts at: 3056 4136 5185 7010 7220 3056
Size: 1080 1049 1825 210 4823
Fragments arranged by size:
4823 1825 1080 1049 210
SbfI CC_TGCA'GG
Cuts at: 1110 6034 1110
Size: 4924 4063
ScaI AGT'ACT
Cuts at: 815 8504 815
Size: 7689 1298
SexAI A'CCwGG_T
Cuts at: 825 1385 825
Size: 560 8427
SfcI C'TryA_G
Cuts at: 33 604 837 1106 3530 5912 6030 6715 7708
Size: 571 233 269 2424 2382 118 685 993
Cuts at: 7708 7899 33
Size: 191 1121
Fragments arranged by size:
2424 2382 1121 993 685 571 269 233
191 118
SfoI GGC'GCC
Cuts at: 6419 6667 6419
Size: 248 8739
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8987
SmaI CCC'GGG
Cuts at: 6009 6009
Size: 8987
SmlI C'TyrA_G
Cuts at: 157 278 641 1144 1182 1196 1538 2481 3684
Size: 121 363 503 38 14 342 943 1203
Cuts at: 3684 3928 6142 7057 7549 7811 157
Size: 244 2214 915 492 262 1333
Fragments arranged by size:
2214 1333 1203 943 915 503 492 363
342 262 244 121 38 14
SpeI A'CTAG_T
Cuts at: 67 4267 67
Size: 4200 4787
SphI G_CATG'C
Cuts at: 6040 6540 6040
Size: 500 8487
StuI AGG'CCT
Cuts at: 163 4774 163
Size: 4611 4376
StyI C'CwwG_G
Cuts at: 746 1738 2906 2977 5813 6121 6475 746
Size: 992 1168 71 2836 308 354 3258
Fragments arranged by size:
3258 2836 1168 992 354 308 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 488 4078 5367 5779 6570 6703 7162 7371 488
Size: 3590 1289 412 791 133 459 209 2104
Fragments arranged by size:
3590 2104 1289 791 459 412 209 133
TatI w'GTAC_w
Cuts at: 463 805 813 3023 4079 8502 463
Size: 342 8 2210 1056 4423 948
Fragments arranged by size:
4423 2210 1056 948 342 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 3917 3949 8504 8536 2103
Size: 32 1782 32 4555 32 2554
Fragments arranged by size:
4555 2554 1782 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1421 2403 3051 6784 1421
Size: 982 648 3733 3624
Fragments arranged by size:
3733 3624 982 648
XbaI T'CTAG_A
Cuts at: 3864 6018 3864
Size: 2154 6833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1782 2692 4698 6482 1782
Size: 910 2006 1784 4287
Fragments arranged by size:
4287 2006 1784 910
XhoI C'TCGA_G
Cuts at: 2481 2481
Size: 8987
XmaI C'CCGG_G
Cuts at: 6007 6007
Size: 8987
XmnI GAAnn'nnTTC
Cuts at: 590 1587 1977 2874 590
Size: 997 390 897 6703
Fragments arranged by size:
6703 997 897 390
ZraI GAC'GTC
Cuts at: 1423 1553 1423
Size: 130 8857
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BcgI
BfrBI BsiWI BssHII BstZ17I ClaI FseI FspAI MfeI
NotI NruI NsiI PacI PasI PflMI PmlI PspOMI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI