Restriction map for
pF1KE3547
(Circular) (Six-base) MAPSORT of: pF1KE3547.seq Check: 7250 from: 1 to: 9302
pF1KE3547 9302 bp
With 182 enzymes: SgfI *
July 9, 2014 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1627 1759 3834 1627
Size: 132 2075 7095
Fragments arranged by size:
7095 2075 132
AatII G_ACGT'C
Cuts at: 3125 4871 5804 3125
Size: 1746 933 6623
Fragments arranged by size:
6623 1746 933
AccI GT'mk_AC
Cuts at: 1476 5303 5492 5798 6340 1476
Size: 3827 189 306 542 4438
Fragments arranged by size:
4438 3827 542 306 189
Acc65I G'GTAC_C
Cuts at: 2095 6318 2095
Size: 4223 5079
AclI AA'CG_TT
Cuts at: 9142 9142
Size: 9302
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1819 2234 2369 3415 3799 4087 4582 7127 7559
Size: 415 135 1046 384 288 495 2545 432
Cuts at: 7559 8306 8574 1819
Size: 747 268 2547
Fragments arranged by size:
2547 2545 1046 747 495 432 415 384
288 268 135
AfeI AGC'GCT
Cuts at: 169 5885 169
Size: 5716 3586
AflIII A'CryG_T
Cuts at: 2973 5360 5660 5753 6654 7758 8735 2973
Size: 2387 300 93 901 1104 977 3540
Fragments arranged by size:
3540 2387 1104 977 901 300 93
AgeI A'CCGG_T
Cuts at: 4022 7690 4022
Size: 3668 5634
AhdI GACnn_n'nnGTC
Cuts at: 1320 2239 2450 2844 5031 6254 1320
Size: 919 211 394 2187 1223 4368
Fragments arranged by size:
4368 2187 1223 919 394 211
AleI CACnn'nnGTG
Cuts at: 3155 3155
Size: 9302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2100 3388 4612 5879 2100
Size: 1288 1224 1267 5523
Fragments arranged by size:
5523 1288 1267 1224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3136 3168 3136
Size: 32 9270
AlwNI CAG_nnn'CTG
Cuts at: 1834 2023 2062 2257 2462 3393 3711 4317 4380
Size: 189 39 195 205 931 318 606 63
Cuts at: 4380 6607 8174 1834
Size: 2227 1567 2962
Fragments arranged by size:
2962 2227 1567 931 606 318 205 195
189 63 39
ApaI G_GGCC'C
Cuts at: 302 460 789 880 2187 2641 2772 2958 302
Size: 158 329 91 1307 454 131 186 6646
Fragments arranged by size:
6646 1307 454 329 186 158 131 91
ApaLI G'TGCA_C
Cuts at: 5815 8072 5815
Size: 2257 7045
ApoI r'AATT_y
Cuts at: 6306 8655 8911 6306
Size: 2349 256 6697
Fragments arranged by size:
6697 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9302
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9302
AvaI C'yCGr_G
Cuts at: 184 257 410 593 788 930 985 1988 2850
Size: 73 153 183 195 142 55 1003 862
Cuts at: 2850 2951 3083 3115 3460 4435 4777 4950 5049
Size: 101 132 32 345 975 342 173 99
Cuts at: 5049 5115 5273 6106 6322 184
Size: 66 158 833 216 3164
Fragments arranged by size:
3164 1003 975 862 833 345 342 216
195 183 173 158 153 142 132 101
99 73 66 55 32
BamHI G'GATC_C
Cuts at: 1848 3743 6327 1848
Size: 1895 2584 4823
Fragments arranged by size:
4823 2584 1895
BanI G'GyrC_C
Cuts at: 120 571 1429 1837 1924 2095 2218 2530 2737
Size: 451 858 408 87 171 123 312 207
Cuts at: 2737 3018 3105 4441 4773 4839 5004 5218 5551
Size: 281 87 1336 332 66 165 214 333
Cuts at: 5551 5917 5979 6146 6318 6732 6980 7015 120
Size: 366 62 167 172 414 248 35 2407
Fragments arranged by size:
2407 1336 858 451 414 408 366 333
332 312 281 248 214 207 172 171
167 165 123 87 87 66 62 35
BanII G_rGCy'C
Cuts at: 302 460 789 880 900 992 1087 1094 1113
Size: 158 329 91 20 92 95 7 19
Cuts at: 1113 1388 1588 1820 2015 2187 2392 2401 2414
Size: 275 200 232 195 172 205 9 13
Cuts at: 2414 2641 2654 2672 2699 2772 2958 3090 3467
Size: 227 13 18 27 73 186 132 377
Cuts at: 3467 3546 4643 4850 5244 5326 5566 5638 6316
Size: 79 1097 207 394 82 240 72 678
Cuts at: 6316 7346 302
Size: 1030 2258
Fragments arranged by size:
2258 1097 1030 678 394 377 329 275
240 232 227 207 205 200 195 186
172 158 132 95 92 91 82 79
73 72 27 20 19 18 13 13
9 7
BbeI G_GCGC'C
Cuts at: 6736 6984 6736
Size: 248 9054
BbsI GAAGACnn'nnnn_
Cuts at: 4181 5027 4181
Size: 846 8456
BbvCI CC'TCA_GC
Cuts at: 190 1561 2324 2424 3221 4819 190
Size: 1371 763 100 797 1598 4673
Fragments arranged by size:
4673 1598 1371 797 763 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 486 520 2542 2576 4861 4895 486
Size: 34 2022 34 2285 34 4893
Fragments arranged by size:
4893 2285 2022 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7200 7961 9302 7200
Size: 761 1341 7200
Fragments arranged by size:
7200 1341 761
BclI T'GATC_A
Cuts at: 3725 6822 3725
Size: 3097 6205
BglI GCCn_nnn'nGGC
Cuts at: 273 1262 3646 3886 4854 273
Size: 989 2384 240 968 4721
Fragments arranged by size:
4721 2384 989 968 240
BglII A'GATC_T
Cuts at: 3443 6817 8854 3443
Size: 3374 2037 3891
Fragments arranged by size:
3891 3374 2037
BlpI GC'TnA_GC
Cuts at: 3851 4699 6414 3851
Size: 848 1715 6739
Fragments arranged by size:
6739 1715 848
Bme1580I G_kGCm'C
Cuts at: 123 302 460 789 880 1929 2187 2223 2641
Size: 179 158 329 91 1049 258 36 418
Cuts at: 2641 2757 2772 2958 2996 4444 4778 5009 5341
Size: 116 15 186 38 1448 334 231 332
Cuts at: 5341 5554 5751 5819 5920 5982 6927 7020 8076
Size: 213 197 68 101 62 945 93 1056
Cuts at: 8076 123
Size: 1349
Fragments arranged by size:
1448 1349 1056 1049 945 418 334 332
329 258 231 213 197 186 179 158
116 101 93 91 68 62 38 36
15
BmgBI CAC'GTC
Cuts at: 4722 6024 4722
Size: 1302 8000
BmrI ACTGGGnnnn_n'
Cuts at: 1980 2369 3166 6514 6595 6930 9074 1980
Size: 389 797 3348 81 335 2144 2208
Fragments arranged by size:
3348 2208 2144 797 389 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 3565 4844 7669 2031
Size: 1534 1279 2825 3664
Fragments arranged by size:
3664 2825 1534 1279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 192 504 840 914 1445 2663 2952 3490
Size: 8 312 336 74 531 1218 289 538
Cuts at: 3490 3511 3841 3934 4435 4814 5076 5238 5395
Size: 21 330 93 501 379 262 162 157
Cuts at: 5395 5674 5697 6145 184
Size: 279 23 448 3341
Fragments arranged by size:
3341 1218 538 531 501 448 379 336
330 312 289 279 262 162 157 93
74 23 21 8
Bpu10I CC'TnA_GC
Cuts at: 190 991 1088 1561 2063 2324 2424 3023 3221
Size: 801 97 473 502 261 100 599 198
Cuts at: 3221 4819 5632 190
Size: 1598 813 3860
Fragments arranged by size:
3860 1598 813 801 599 502 473 261
198 100 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1288 1693 3649 5305 6478 7357 7849 8147
Size: 1048 405 1956 1656 1173 879 492 298
Cuts at: 8147 240
Size: 1395
Fragments arranged by size:
1956 1656 1395 1173 1048 879 492 405
298
BsaI GGTCTCn'nnnn_
Cuts at: 676 1291 1510 2194 4031 5104 5134 9006 676
Size: 615 219 684 1837 1073 30 3872 972
Fragments arranged by size:
3872 1837 1073 972 684 615 219 30
BsaAI yAC'GTr
Cuts at: 3315 4680 5361 5670 5754 6655 3315
Size: 1365 681 309 84 901 5962
Fragments arranged by size:
5962 1365 901 681 309 84
BsaBI GATnn'nnATC
Cuts at: 6 6816 8853 6
Size: 6810 2037 455
Fragments arranged by size:
6810 2037 455
BsaHI Gr'CG_yC
Cuts at: 292 422 616 3122 4478 4868 4966 5801 6733
Size: 130 194 2506 1356 390 98 835 932
Cuts at: 6733 6981 7683 9179 292
Size: 248 702 1496 415
Fragments arranged by size:
2506 1496 1356 932 835 702 415 390
248 194 130 98
BsaWI w'CCGG_w
Cuts at: 3154 4022 4842 6491 7012 7690 7964 8111 8522
Size: 868 820 1649 521 678 274 147 411
Cuts at: 8522 3154
Size: 3934
Fragments arranged by size:
3934 1649 868 820 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 3136 3166 5308 5338 5356 5386 5471
Size: 30 2724 30 2142 30 18 30 85
Cuts at: 5471 5501 5647 5677 5893 5923 6121 6151 382
Size: 30 146 30 216 30 198 30 3533
Fragments arranged by size:
3533 2724 2142 216 198 146 85 30
30 30 30 30 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 482 792 795 798 877 886 918 1223
Size: 271 310 3 3 79 9 32 305
Cuts at: 1223 1800 1858 2023 2955 3898 4799 4836 5079
Size: 577 58 165 932 943 901 37 243
Cuts at: 5079 5122 5170 5173 5798 5914 6271 211
Size: 43 48 3 625 116 357 3242
Fragments arranged by size:
3242 943 932 901 625 577 357 310
305 271 243 165 116 79 58 48
43 37 32 9 3 3 3
BseYI C'CCAG_C
Cuts at: 131 403 459 478 529 559 971 1343 1375
Size: 272 56 19 51 30 412 372 32
Cuts at: 1375 1674 1680 2599 2620 2883 3527 5041 5334
Size: 299 6 919 21 263 644 1514 293
Cuts at: 5334 6727 7485 8062 8558 131
Size: 1393 758 577 496 875
Fragments arranged by size:
1514 1393 919 875 758 644 577 496
412 372 299 293 272 263 56 51
32 30 21 19 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 3133 3191 3757 4041 1903
Size: 1230 58 566 284 7164
Fragments arranged by size:
7164 1230 566 284 58
BsiEI CG_ry'CG
Cuts at: 104 244 437 452 706 948 4007 6844 6890
Size: 140 193 15 254 242 3059 2837 46
Cuts at: 6890 8098 104
Size: 1208 1308
Fragments arranged by size:
3059 2837 1308 1208 254 242 193 140
46 15
BsiHKAI G_wGCw'C
Cuts at: 1588 1797 1820 2015 3419 3467 3476 5326 5660
Size: 209 23 195 1404 48 9 1850 334
Cuts at: 5660 5819 6131 6316 7094 7284 8076 1588
Size: 159 312 185 778 190 792 2814
Fragments arranged by size:
2814 1850 1404 792 778 334 312 209
195 190 185 159 48 23 9
BsmBI CGTCTCn'nnnn_
Cuts at: 153 407 944 2909 4828 6254 153
Size: 254 537 1965 1919 1426 3201
Fragments arranged by size:
3201 1965 1919 1426 537 254
Bsp1286I G_dGCh'C
Cuts at: 123 302 460 789 880 900 992 1087 1094
Size: 179 158 329 91 20 92 95 7
Cuts at: 1094 1113 1388 1588 1797 1820 1929 2015 2187
Size: 19 275 200 209 23 109 86 172
Cuts at: 2187 2223 2392 2401 2414 2641 2654 2672 2699
Size: 36 169 9 13 227 13 18 27
Cuts at: 2699 2757 2772 2958 2996 3090 3419 3467 3476
Size: 58 15 186 38 94 329 48 9
Cuts at: 3476 3546 4444 4643 4778 4850 5009 5244 5326
Size: 70 898 199 135 72 159 235 82
Cuts at: 5326 5341 5554 5566 5638 5660 5751 5819 5920
Size: 15 213 12 72 22 91 68 101
Cuts at: 5920 5982 6131 6316 6927 7020 7094 7284 7346
Size: 62 149 185 611 93 74 190 62
Cuts at: 7346 8076 123
Size: 730 1349
Fragments arranged by size:
1349 898 730 611 329 329 275 235
227 213 209 200 199 190 186 185
179 172 169 159 158 149 135 109
101 95 94 93 92 91 91 86
82 74 72 72 70 68 62 62
58 48 38 36 27 23 22 20
19 18 15 15 13 13 12 9
9 7
BspEI T'CCGG_A
Cuts at: 8522 8522
Size: 9302
BspHI T'CATG_A
Cuts at: 3722 4577 8478 3722
Size: 855 3901 4546
Fragments arranged by size:
4546 3901 855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1627 1759 3155 3486 3834 5300 5440 6074 6352
Size: 132 1396 331 348 1466 140 634 278
Cuts at: 6352 6566 6868 7249 1627
Size: 214 302 381 3680
Fragments arranged by size:
3680 1466 1396 634 381 348 331 302
278 214 140 132
BsrBI CCG'CTC
Cuts at: 50 176 330 723 1358 5252 7594 7648 9132
Size: 126 154 393 635 3894 2342 54 1484
Cuts at: 9132 9297 50
Size: 165 55
Fragments arranged by size:
3894 2342 1484 635 393 165 154 126
55 54
BsrDI GCAATG_nn'
Cuts at: 2283 4940 5109 7214 2283
Size: 2657 169 2105 4371
Fragments arranged by size:
4371 2657 2105 169
BsrFI r'CCGG_y
Cuts at: 239 701 4022 5075 6016 7300 7481 7690 239
Size: 462 3321 1053 941 1284 181 209 1851
Fragments arranged by size:
3321 1851 1284 1053 941 462 209 181
BsrGI T'GTAC_A
Cuts at: 4109 4109
Size: 9302
BssHII G'CGCG_C
Cuts at: 4707 4707
Size: 9302
BssSI C'ACGA_G
Cuts at: 234 3478 7573 7931 234
Size: 3244 4095 358 1605
Fragments arranged by size:
4095 3244 1605 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3138 3281 3510 3625 3684 4760 5823 6679 3138
Size: 143 229 115 59 1076 1063 856 5761
Fragments arranged by size:
5761 1076 1063 856 229 143 115 59
BstBI TT'CG_AA
Cuts at: 7663 7663
Size: 9302
BstEII G'GTnAC_C
Cuts at: 4984 4984
Size: 9302
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 5229 2155
Size: 3074 6228
BstYI r'GATC_y
Cuts at: 1 1848 1910 3443 3455 3743 3945 6327 6794
Size: 1847 62 1533 12 288 202 2382 467
Cuts at: 6794 6817 7152 7398 8410 8496 8508 8854 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2382 1847 1533 1012 467 449 346 335
288 246 202 86 62 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 2777 3236 3791 4649 5309 2777
Size: 459 555 858 660 6770
Fragments arranged by size:
6770 858 660 555 459
BtgI C'CryG_G
Cuts at: 107 248 431 437 915 1269 1596 1766 2148
Size: 141 183 6 478 354 327 170 382
Cuts at: 2148 2429 2607 5132 5259 5974 107
Size: 281 178 2525 127 715 3435
Fragments arranged by size:
3435 2525 715 478 382 354 327 281
183 178 170 141 127 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 798 1961 3095 4719 7252 7433 7596 798
Size: 1163 1134 1624 2533 181 163 2504
Fragments arranged by size:
2533 2504 1624 1163 1134 181 163
BtsI GCAGTG_nn'
Cuts at: 942 2481 2798 3504 4327 4596 6231 6249 6545
Size: 1539 317 706 823 269 1635 18 296
Cuts at: 6545 942
Size: 3699
Fragments arranged by size:
3699 1635 1539 823 706 317 296 269
18
ClaI AT'CG_AT
Cuts at: 3602 3602
Size: 9302
DraI TTT'AAA
Cuts at: 6301 6301
Size: 9302
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 9302
DrdI GACnn_nn'nnGTC
Cuts at: 421 4051 7008 7866 421
Size: 3630 2957 858 1857
Fragments arranged by size:
3630 2957 1857 858
EaeI y'GGCC_r
Cuts at: 241 305 434 449 703 921 963 1223 1250
Size: 64 129 15 254 218 42 260 27
Cuts at: 1250 3317 3638 5471 6003 6018 6887 7061 7452
Size: 2067 321 1833 532 15 869 174 391
Cuts at: 7452 7479 8824 241
Size: 27 1345 719
Fragments arranged by size:
2067 1833 1345 869 719 532 391 321
260 254 218 174 129 64 42 27
27 15 15
EagI C'GGCC_G
Cuts at: 241 434 449 703 6887 241
Size: 193 15 254 6184 2656
Fragments arranged by size:
6184 2656 254 193 15
EarI CTCTTCn'nnn_
Cuts at: 508 863 2345 2474 4036 7325 7535 508
Size: 355 1482 129 1562 3289 210 2275
Fragments arranged by size:
3289 2275 1562 1482 355 210 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 1142 2215 2851 3904 4737 7510 7820 7966
Size: 1015 1073 636 1053 833 2773 310 146
Cuts at: 7966 9112 127
Size: 1146 317
Fragments arranged by size:
2773 1146 1073 1053 1015 833 636 317
310 146
EcoICRI GAG'CTC
Cuts at: 1586 1818 2013 3465 5324 6314 1586
Size: 232 195 1452 1859 990 4574
Fragments arranged by size:
4574 1859 1452 990 232 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 192 504 840 914 1445 1819 2234 2369
Size: 8 312 336 74 531 374 415 135
Cuts at: 2369 2663 2952 3415 3490 3511 3799 3841 3934
Size: 294 289 463 75 21 288 42 93
Cuts at: 3934 4087 4435 4582 4814 5076 5238 5395 5674
Size: 153 348 147 232 262 162 157 279
Cuts at: 5674 5697 6145 7127 7559 8306 8574 184
Size: 23 448 982 432 747 268 912
Fragments arranged by size:
982 912 747 531 463 448 432 415
374 348 336 312 294 289 288 279
268 262 232 162 157 153 147 135
93 75 74 42 23 21 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2776 3373 3493 3820 3976 4249 4978 6004 2776
Size: 597 120 327 156 273 729 1026 6074
Fragments arranged by size:
6074 1026 729 597 327 273 156 120
EcoO109I rG'GnC_Cy
Cuts at: 299 407 456 785 876 1168 1420 1831 2020
Size: 108 49 329 91 292 252 411 189
Cuts at: 2020 2184 2303 2524 2637 2638 2768 2769 3162
Size: 164 119 221 113 1 130 1 393
Cuts at: 3162 3771 3954 4254 4335 4359 5289 5520 6103
Size: 609 183 300 81 24 930 231 583
Cuts at: 6103 6154 6441 299
Size: 51 287 3160
Fragments arranged by size:
3160 930 609 583 411 393 329 300
292 287 252 231 221 189 183 164
130 119 113 108 91 81 51 49
24 1 1
EcoRI G'AATT_C
Cuts at: 6306 6306
Size: 9302
EcoRV GAT'ATC
Cuts at: 1506 2494 1506
Size: 988 8314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1251 7321 1251
Size: 6070 3232
FspI TGC'GCA
Cuts at: 496 3364 7083 496
Size: 2868 3719 2715
Fragments arranged by size:
3719 2868 2715
HaeII r_GCGC'y
Cuts at: 171 372 536 2331 2883 3712 3897 4381 5887
Size: 201 164 1795 552 829 185 484 1506
Cuts at: 5887 6736 6984 8006 8985 171
Size: 849 248 1022 979 488
Fragments arranged by size:
1795 1506 1022 979 849 829 552 488
484 248 201 185 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 382 414 529 561 1306 1338 1495 1527 1615
Size: 32 115 32 745 32 157 32 88
Cuts at: 1615 1647 1710 1742 2225 2257 2436 2468 2830
Size: 32 63 32 483 32 179 32 362
Cuts at: 2830 2862 3909 3941 5005 5017 5037 5049 5612
Size: 32 1047 32 1064 12 20 12 563
Cuts at: 5612 5644 6240 6272 7144 7174 7176 7206 382
Size: 32 596 32 872 30 2 30 2478
Fragments arranged by size:
2478 1064 1047 872 745 596 563 483
362 179 157 115 88 63 32 32
32 32 32 32 32 32 32 32
32 32 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 1477 5304 6341 1477
Size: 3827 1037 4438
Fragments arranged by size:
4438 3827 1037
KasI G'GCGC_C
Cuts at: 6732 6980 6732
Size: 248 9054
KpnI G_GTAC'C
Cuts at: 2099 6322 2099
Size: 4223 5079
MluI A'CGCG_T
Cuts at: 8735 8735
Size: 9302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4346 5399 6240 7973 8157 4346
Size: 1053 841 1733 184 5491
Fragments arranged by size:
5491 1733 1053 841 184
MscI TGG'CCA
Cuts at: 1225 1252 3319 3640 5473 7063 1225
Size: 27 2067 321 1833 1590 3464
Fragments arranged by size:
3464 2067 1833 1590 321 27
MslI CAynn'nnrTG
Cuts at: 490 2104 2987 3004 3155 3370 3902 3994 5575
Size: 1614 883 17 151 215 532 92 1581
Cuts at: 5575 7291 7418 490
Size: 1716 127 2374
Fragments arranged by size:
2374 1716 1614 1581 883 532 215 151
127 92 17
MspA1I CmG'CkG
Cuts at: 403 433 439 739 888 971 1271 1482 1854
Size: 30 6 300 149 83 300 211 372
Cuts at: 1854 1998 2715 3393 3741 3831 4170 4345 4631
Size: 144 717 678 348 90 339 175 286
Cuts at: 4631 4879 5652 5690 5787 5969 6048 6413 6727
Size: 248 773 38 97 182 79 365 314
Cuts at: 6727 7087 8100 8345 403
Size: 360 1013 245 1360
Fragments arranged by size:
1360 1013 773 717 678 372 365 360
348 339 314 300 300 286 248 245
211 182 175 149 144 97 90 83
79 38 30 6
NaeI GCC'GGC
Cuts at: 241 703 7483 241
Size: 462 6780 2060
Fragments arranged by size:
6780 2060 462
NarI GG'CG_CC
Cuts at: 6733 6981 6733
Size: 248 9054
NcoI C'CATG_G
Cuts at: 107 1766 2148 2429 107
Size: 1659 382 281 6980
Fragments arranged by size:
6980 1659 382 281
NgoMIV G'CCGG_C
Cuts at: 239 701 7481 239
Size: 462 6780 2060
Fragments arranged by size:
6780 2060 462
NotI GC'GGCC_GC
Cuts at: 434 434
Size: 9302
NspI r_CATG'y
Cuts at: 635 3141 3189 3306 3387 3513 3526 3687 4599
Size: 2506 48 117 81 126 13 161 912
Cuts at: 4599 5419 5664 6355 6855 7762 635
Size: 820 245 691 500 907 2175
Fragments arranged by size:
2506 2175 912 907 820 691 500 245
161 126 117 81 48 13
PasI CC'CwG_GG
Cuts at: 2155 2535 4266 5556 2155
Size: 380 1731 1290 5901
Fragments arranged by size:
5901 1731 1290 380
PciI A'CATG_T
Cuts at: 5660 7758 5660
Size: 2098 7204
PflMI CCAn_nnn'nTGG
Cuts at: 742 1920 4317 5188 5233 742
Size: 1178 2397 871 45 4811
Fragments arranged by size:
4811 2397 1178 871 45
PfoI T'CCnGG_A
Cuts at: 520 1130 1868 3467 3488 520
Size: 610 738 1599 21 6334
Fragments arranged by size:
6334 1599 738 610 21
PmeI GTTT'AAAC
Cuts at: 6301 6301
Size: 9302
PmlI CAC'GTG
Cuts at: 3315 5361 5754 3315
Size: 2046 393 6863
Fragments arranged by size:
6863 2046 393
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3136 3168 8441 8473 3136
Size: 32 5273 32 3965
Fragments arranged by size:
5273 3965 32 32
PpuMI rG'GwC_Cy
Cuts at: 407 1168 1420 2303 3162 3771 4254 4335 4359
Size: 761 252 883 859 609 483 81 24
Cuts at: 4359 5289 5520 407
Size: 930 231 4189
Fragments arranged by size:
4189 930 883 859 761 609 483 252
231 81 24
PspOMI G'GGCC_C
Cuts at: 298 456 785 876 2183 2637 2768 2954 298
Size: 158 329 91 1307 454 131 186 6646
Fragments arranged by size:
6646 1307 454 329 186 158 131 91
PspXI vC'TCGA_Gb
Cuts at: 3115 3115
Size: 9302
PstI C_TGCA'G
Cuts at: 1027 3255 3286 3914 4787 4884 5063 5651 6042
Size: 2228 31 628 873 97 179 588 391
Cuts at: 6042 6349 7034 1027
Size: 307 685 3295
Fragments arranged by size:
3295 2228 873 685 628 588 391 307
179 97 31
PvuI CG_AT'CG
Cuts at: 104 4007 104
Size: 3903 5399
PvuII CAG'CTG
Cuts at: 888 4345 4631 5652 5787 5969 6048 6727 7087
Size: 3457 286 1021 135 182 79 679 360
Cuts at: 7087 888
Size: 3103
Fragments arranged by size:
3457 3103 1021 679 360 286 182 135
79
RsrII CG'GwC_CG
Cuts at: 7497 7497
Size: 9302
SacI G_AGCT'C
Cuts at: 1588 1820 2015 3467 5326 6316 1588
Size: 232 195 1452 1859 990 4574
Fragments arranged by size:
4574 1859 1452 990 232 195
SacII CC_GC'GG
Cuts at: 434 440 1272 434
Size: 6 832 8464
Fragments arranged by size:
8464 832 6
SalI G'TCGA_C
Cuts at: 1475 5302 6339 1475
Size: 3827 1037 4438
Fragments arranged by size:
4438 3827 1037
SanDI GG'GwC_CC
Cuts at: 407 5520 407
Size: 5113 4189
SapI GCTCTTCn'nnn_
Cuts at: 508 2345 7325 7535 508
Size: 1837 4980 210 2275
Fragments arranged by size:
4980 2275 1837 210
SbfI CC_TGCA'GG
Cuts at: 6349 6349
Size: 9302
ScaI AGT'ACT
Cuts at: 8819 8819
Size: 9302
SexAI A'CCwGG_T
Cuts at: 1575 2214 2733 5453 1575
Size: 639 519 2720 5424
Fragments arranged by size:
5424 2720 639 519
SfcI C'TryA_G
Cuts at: 33 1023 2298 3251 3282 3910 4783 4880 5059
Size: 990 1275 953 31 628 873 97 179
Cuts at: 5059 5647 6038 6345 7030 8023 8214 33
Size: 588 391 307 685 993 191 1121
Fragments arranged by size:
1275 1121 993 990 953 873 685 628
588 391 307 191 179 97 31
SfoI GGC'GCC
Cuts at: 6734 6982 6734
Size: 248 9054
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9302
SmaI CCC'GGG
Cuts at: 186 259 790 987 4779 5275 6324 186
Size: 73 531 197 3792 496 1049 3164
Fragments arranged by size:
3792 3164 1049 531 496 197 73
SmlI C'TyrA_G
Cuts at: 219 1303 1708 3115 3664 5284 6457 7372 7864
Size: 1084 405 1407 549 1620 1173 915 492
Cuts at: 7864 8126 219
Size: 262 1395
Fragments arranged by size:
1620 1407 1395 1173 1084 915 549 492
405 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9302
SphI G_CATG'C
Cuts at: 3513 3526 3687 4599 5419 6355 6855 3513
Size: 13 161 912 820 936 500 5960
Fragments arranged by size:
5960 936 912 820 500 161 13
SrfI GCCC'GGGC
Cuts at: 790 987 4779 790
Size: 197 3792 5313
Fragments arranged by size:
5313 3792 197
StuI AGG'CCT
Cuts at: 996 1029 2296 3654 4501 4729 5314 5999 6171
Size: 33 1267 1358 847 228 585 685 172
Cuts at: 6171 996
Size: 4127
Fragments arranged by size:
4127 1358 1267 847 685 585 228 172
33
StyI C'CwwG_G
Cuts at: 107 1226 1766 2148 2429 3566 3641 4296 4485
Size: 1119 540 382 281 1137 75 655 189
Cuts at: 4485 5123 5546 6142 6436 6790 107
Size: 638 423 596 294 354 2619
Fragments arranged by size:
2619 1137 1119 655 638 596 540 423
382 354 294 281 189 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 700 962 1912 5569 5578 5710 5718 5901 6885
Size: 262 950 3657 9 132 8 183 984
Cuts at: 6885 7018 7477 7686 700
Size: 133 459 209 2316
Fragments arranged by size:
3657 2316 984 950 459 262 209 183
133 132 9 8
TatI w'GTAC_w
Cuts at: 3134 4109 4145 8817 3134
Size: 975 36 4672 3619
Fragments arranged by size:
4672 3619 975 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5471 5503 5893 5925 6121 6153 8819 8851 5471
Size: 32 390 32 196 32 2666 32 5922
Fragments arranged by size:
5922 2666 390 196 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7099 7099
Size: 9302
XbaI T'CTAG_A
Cuts at: 4545 6333 4545
Size: 1788 7514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1936 2152 2427 3327 4521 5574 6052 6797 1936
Size: 216 275 900 1194 1053 478 745 4441
Fragments arranged by size:
4441 1194 1053 900 745 478 275 216
XhoI C'TCGA_G
Cuts at: 3115 3115
Size: 9302
XmaI C'CCGG_G
Cuts at: 184 257 788 985 4777 5273 6322 184
Size: 73 531 197 3792 496 1049 3164
Fragments arranged by size:
3792 3164 1049 531 496 197 73
ZraI GAC'GTC
Cuts at: 3123 4869 5802 3123
Size: 1746 933 6623
Fragments arranged by size:
6623 1746 933
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BfrBI BmtI BsiWI BsmI
BstZ17I CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PshAI PsiI PsrI SfiI
SgrAI SnaBI SspI SwaI XmnI