Restriction map for
pF1KE3526
(Circular) (Six-base) MAPSORT of: pF1KE3526.seq Check: 7130 from: 1 to: 6221
pF1KE3526 6221 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1911 1911
Size: 6221
AatII G_ACGT'C
Cuts at: 262 1682 2042 262
Size: 1420 360 4441
Fragments arranged by size:
4441 1420 360
AccI GT'mk_AC
Cuts at: 3259 3259
Size: 6221
Acc65I G'GTAC_C
Cuts at: 665 3237 665
Size: 2572 3649
AclI AA'CG_TT
Cuts at: 6061 6061
Size: 6221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 400 1930 2698 3094 4046 4478 5225 5493
Size: 261 1530 768 396 952 432 747 268
Cuts at: 5493 139
Size: 867
Fragments arranged by size:
1530 952 867 768 747 432 396 268
261
AfeI AGC'GCT
Cuts at: 1067 1504 1067
Size: 437 5784
AflIII A'CryG_T
Cuts at: 1454 3573 4677 5654 1454
Size: 2119 1104 977 2021
Fragments arranged by size:
2119 2021 1104 977
AgeI A'CCGG_T
Cuts at: 4609 4609
Size: 6221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2081 2480 2081
Size: 399 5822
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1792 1824 1792
Size: 32 6189
AlwNI CAG_nnn'CTG
Cuts at: 1416 2287 2952 3526 5093 1416
Size: 871 665 574 1567 2544
Fragments arranged by size:
2544 1567 871 665 574
ApaI G_GGCC'C
Cuts at: 706 2035 2623 2707 2797 706
Size: 1329 588 84 90 4130
Fragments arranged by size:
4130 1329 588 90 84
ApaLI G'TGCA_C
Cuts at: 214 874 2692 4991 214
Size: 660 1818 2299 1444
Fragments arranged by size:
2299 1818 1444 660
ApoI r'AATT_y
Cuts at: 1432 3225 5574 5830 1432
Size: 1793 2349 256 1823
Fragments arranged by size:
2349 1823 1793 256
AscI GG'CGCG_CC
Cuts at: 2180 2180
Size: 6221
AseI AT'TA_AT
Cuts at: 20 20
Size: 6221
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
AvaI C'yCGr_G
Cuts at: 823 1528 1914 2096 3241 823
Size: 705 386 182 1145 3803
Fragments arranged by size:
3803 1145 705 386 182
AvrII C'CTAG_G
Cuts at: 1047 1047
Size: 6221
BamHI G'GATC_C
Cuts at: 1098 1667 3246 1098
Size: 569 1579 4073
Fragments arranged by size:
4073 1579 569
BanI G'GyrC_C
Cuts at: 665 2167 2205 2448 3237 3651 3899 3934 665
Size: 1502 38 243 789 414 248 35 2952
Fragments arranged by size:
2952 1502 789 414 248 243 38 35
BanII G_rGCy'C
Cuts at: 706 830 1586 1753 2035 2311 2623 2707 2722
Size: 124 756 167 282 276 312 84 15
Cuts at: 2722 2797 2840 3235 4265 706
Size: 75 43 395 1030 2662
Fragments arranged by size:
2662 1030 756 395 312 282 276 167
124 84 75 43 15
BbeI G_GCGC'C
Cuts at: 3655 3903 3655
Size: 248 5973
BbsI GAAGACnn'nnnn_
Cuts at: 1191 2786 1191
Size: 1595 4626
BbvCI CC'TCA_GC
Cuts at: 181 181
Size: 6221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6187
BciVI GTATCCnnnnn_n'
Cuts at: 1653 4119 4880 6221 1653
Size: 2466 761 1341 1653
Fragments arranged by size:
2466 1653 1341 761
BclI T'GATC_A
Cuts at: 3741 3741
Size: 6221
BfrBI ATG'CAT
Cuts at: 3213 3213
Size: 6221
BglI GCCn_nnn'nGGC
Cuts at: 637 994 2190 637
Size: 357 1196 4668
Fragments arranged by size:
4668 1196 357
BglII A'GATC_T
Cuts at: 1517 3736 5773 1517
Size: 2219 2037 1965
Fragments arranged by size:
2219 2037 1965
BlpI GC'TnA_GC
Cuts at: 148 299 782 2072 2554 2839 3333 148
Size: 151 483 1290 482 285 494 3036
Fragments arranged by size:
3036 1290 494 483 482 285 151
Bme1580I G_kGCm'C
Cuts at: 218 706 878 2035 2623 2696 2707 2797 3189
Size: 488 172 1157 588 73 11 90 392
Cuts at: 3189 3846 3939 4995 218
Size: 657 93 1056 1444
Fragments arranged by size:
1444 1157 1056 657 588 488 392 172
93 90 73 11
BmrI ACTGGGnnnn_n'
Cuts at: 1660 2101 2618 3433 3514 3849 5993 1660
Size: 441 517 815 81 335 2144 1888
Fragments arranged by size:
2144 1888 815 517 441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 147 1554 2037 4588 147
Size: 1407 483 2551 1780
Fragments arranged by size:
2551 1780 1407 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 1572 1648 1972 2923 2980 2998 1534
Size: 38 76 324 951 57 18 4757
Fragments arranged by size:
4757 951 324 76 57 38 18
Bpu10I CC'TnA_GC
Cuts at: 181 2305 2821 181
Size: 2124 516 3581
Fragments arranged by size:
3581 2124 516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 997 1132 2565 3397 4276 4768 5066 466
Size: 531 135 1433 832 879 492 298 1621
Fragments arranged by size:
1621 1433 879 832 531 492 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 562 1525 2908 5925 562
Size: 963 1383 3017 858
Fragments arranged by size:
3017 1383 963 858
BsaAI yAC'GTr
Cuts at: 3574 3574
Size: 6221
BsaBI GATnn'nnATC
Cuts at: 6 721 3735 5772 6
Size: 715 3014 2037 455
Fragments arranged by size:
3014 2037 715 455
BsaHI Gr'CG_yC
Cuts at: 259 679 1043 1679 1994 2039 2539 2596 3652
Size: 420 364 636 315 45 500 57 1056
Cuts at: 3652 3900 4602 6098 259
Size: 248 702 1496 382
Fragments arranged by size:
1496 1056 702 636 500 420 382 364
315 248 57 45
BsaWI w'CCGG_w
Cuts at: 1810 3410 3931 4609 4883 5030 5441 1810
Size: 1600 521 678 274 147 411 2590
Fragments arranged by size:
2590 1600 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 1723 1753 649
Size: 30 1044 30 5117
Fragments arranged by size:
5117 1044 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 868 1018 2341 2389 2493 2920 2950 2995 3004
Size: 150 1323 48 104 427 30 45 9
Cuts at: 3004 868
Size: 4085
Fragments arranged by size:
4085 1323 427 150 104 48 45 30
9
BseYI C'CCAG_C
Cuts at: 154 1102 1317 1774 2195 2349 3194 3646 4404
Size: 948 215 457 421 154 845 452 758
Cuts at: 4404 4981 5477 154
Size: 577 496 898
Fragments arranged by size:
948 898 845 758 577 496 457 452
421 215 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 1266 1579 836
Size: 430 313 5478
Fragments arranged by size:
5478 430 313
BsiEI CG_ry'CG
Cuts at: 104 1118 1260 2535 3763 3809 5017 104
Size: 1014 142 1275 1228 46 1208 1308
Fragments arranged by size:
1308 1275 1228 1208 1014 142 46
BsiHKAI G_wGCw'C
Cuts at: 218 374 458 512 788 878 892 962 1114
Size: 156 84 54 276 90 14 70 152
Cuts at: 1114 1136 1586 1699 2311 2560 2696 2840 3235
Size: 22 450 113 612 249 136 144 395
Cuts at: 3235 4013 4203 4995 218
Size: 778 190 792 1444
Fragments arranged by size:
1444 792 778 612 450 395 276 249
190 156 152 144 136 113 90 84
70 54 22 14
BsmBI CGTCTCn'nnnn_
Cuts at: 1844 2932 1844
Size: 1088 5133
Bsp1286I G_dGCh'C
Cuts at: 218 374 458 512 706 788 830 878 892
Size: 156 84 54 194 82 42 48 14
Cuts at: 892 962 1114 1136 1586 1699 1753 2035 2311
Size: 70 152 22 450 113 54 282 276
Cuts at: 2311 2560 2623 2696 2707 2722 2797 2840 3189
Size: 249 63 73 11 15 75 43 349
Cuts at: 3189 3235 3846 3939 4013 4203 4265 4995 218
Size: 46 611 93 74 190 62 730 1444
Fragments arranged by size:
1444 730 611 450 349 282 276 249
194 190 156 152 113 93 84 82
75 74 73 70 63 62 54 54
48 46 43 42 22 15 14 11
BspEI T'CCGG_A
Cuts at: 5441 5441
Size: 6221
BspHI T'CATG_A
Cuts at: 1463 3179 5397 1463
Size: 1716 2218 2287
Fragments arranged by size:
2287 2218 1716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 416 633 1043 1355 1911 2558 3038 3271 3485
Size: 217 410 312 556 647 480 233 214
Cuts at: 3485 3787 4168 416
Size: 302 381 2469
Fragments arranged by size:
2469 647 556 480 410 381 312 302
233 217 214
BsrBI CCG'CTC
Cuts at: 50 303 334 1278 2211 2994 4513 4567 6051
Size: 253 31 944 933 783 1519 54 1484
Cuts at: 6051 6216 50
Size: 165 55
Fragments arranged by size:
1519 1484 944 933 783 253 165 55
54 31
BsrDI GCAATG_nn'
Cuts at: 3146 4133 3146
Size: 987 5234
BsrFI r'CCGG_y
Cuts at: 287 359 1549 2091 2189 4219 4400 4609 287
Size: 72 1190 542 98 2030 181 209 1899
Fragments arranged by size:
2030 1899 1190 542 209 181 98 72
BsrGI T'GTAC_A
Cuts at: 767 767
Size: 6221
BssHII G'CGCG_C
Cuts at: 730 2180 730
Size: 1450 4771
BssSI C'ACGA_G
Cuts at: 136 1230 4492 4850 136
Size: 1094 3262 358 1507
Fragments arranged by size:
3262 1507 1094 358
BstAPI GCAn_nnn'nTGC
Cuts at: 882 1602 3598 882
Size: 720 1996 3505
Fragments arranged by size:
3505 1996 720
BstBI TT'CG_AA
Cuts at: 4582 4582
Size: 6221
BstEII G'GTnAC_C
Cuts at: 1404 1832 1404
Size: 428 5793
BstXI CCAn_nnnn'nTGG
Cuts at: 1734 2730 2901 1734
Size: 996 171 5054
Fragments arranged by size:
5054 996 171
BstYI r'GATC_y
Cuts at: 1 1098 1517 1667 3246 3713 3736 4071 4317
Size: 1097 419 150 1579 467 23 335 246
Cuts at: 4317 5329 5415 5427 5773 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1579 1097 1012 467 449 419 346 335
246 150 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1672 2635 1672
Size: 963 5258
BtgI C'CryG_G
Cuts at: 470 758 1204 1976 2129 2265 2369 2444 2452
Size: 288 446 772 153 136 104 75 8
Cuts at: 2452 2529 2683 3059 470
Size: 77 154 376 3632
Fragments arranged by size:
3632 772 446 376 288 154 153 136
104 77 75 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1380 4171 4352 4515 1380
Size: 2791 181 163 3086
Fragments arranged by size:
3086 2791 181 163
BtsI GCAGTG_nn'
Cuts at: 165 1373 1600 2363 2696 3464 165
Size: 1208 227 763 333 768 2922
Fragments arranged by size:
2922 1208 768 763 333 227
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1193 1228 1193
Size: 35 6186
DraI TTT'AAA
Cuts at: 3220 3220
Size: 6221
DraIII CAC_nnn'GTG
Cuts at: 2266 2266
Size: 6221
DrdI GACnn_nn'nnGTC
Cuts at: 3927 4785 3927
Size: 858 5363
EaeI y'GGCC_r
Cuts at: 231 638 986 1115 1257 1418 1952 2081 2089
Size: 407 348 129 142 161 534 129 8
Cuts at: 2089 2126 2532 3806 3980 4371 4398 5743 231
Size: 37 406 1274 174 391 27 1345 709
Fragments arranged by size:
1345 1274 709 534 407 406 391 348
174 161 142 129 129 37 27 8
EagI C'GGCC_G
Cuts at: 1115 1257 2532 3806 1115
Size: 142 1275 1274 3530
Fragments arranged by size:
3530 1275 1274 142
EarI CTCTTCn'nnn_
Cuts at: 188 1303 1345 2655 4244 4454 188
Size: 1115 42 1310 1589 210 1955
Fragments arranged by size:
1955 1589 1310 1115 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2486 4429 4739 4885 6031 2486
Size: 1943 310 146 1146 2676
Fragments arranged by size:
2676 1943 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1584 2309 2838 3233 1584
Size: 725 529 395 4572
Fragments arranged by size:
4572 725 529 395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 400 1534 1572 1648 1930 1972 2698 2923
Size: 261 1134 38 76 282 42 726 225
Cuts at: 2923 2980 2998 3094 4046 4478 5225 5493 139
Size: 57 18 96 952 432 747 268 867
Fragments arranged by size:
1134 952 867 747 726 432 282 268
261 225 96 76 57 42 38 18
EcoNI CCTnn'n_nnAGG
Cuts at: 232 1233 1351 232
Size: 1001 118 5102
Fragments arranged by size:
5102 1001 118
EcoO109I rG'GnC_Cy
Cuts at: 405 702 703 1654 1880 2032 2620 2703 2704
Size: 297 1 951 226 152 588 83 1
Cuts at: 2704 2787 2856 3360 405
Size: 83 69 504 3266
Fragments arranged by size:
3266 951 588 504 297 226 152 83
83 69 1 1
EcoRI G'AATT_C
Cuts at: 3225 3225
Size: 6221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4240 4240
Size: 6221
FseI GG_CCGG'CC
Cuts at: 2095 2193 2095
Size: 98 6123
FspI TGC'GCA
Cuts at: 541 4002 541
Size: 3461 2760
HaeII r_GCGC'y
Cuts at: 175 1069 1292 1506 3655 3903 4925 5904 175
Size: 894 223 214 2149 248 1022 979 492
Fragments arranged by size:
2149 1022 979 894 492 248 223 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 701 733 847 879 1723 1755 2269 2301 4063
Size: 32 114 32 844 32 514 32 1762
Cuts at: 4063 4093 4095 4125 701
Size: 30 2 30 2797
Fragments arranged by size:
2797 1762 844 514 114 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3260 3260
Size: 6221
KasI G'GCGC_C
Cuts at: 3651 3899 3651
Size: 248 5973
KpnI G_GTAC'C
Cuts at: 669 3241 669
Size: 2572 3649
MluI A'CGCG_T
Cuts at: 5654 5654
Size: 6221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1547 1867 3137 4892 5076 1547
Size: 320 1270 1755 184 2692
Fragments arranged by size:
2692 1755 1270 320 184
MscI TGG'CCA
Cuts at: 233 988 1420 1954 2128 3982 233
Size: 755 432 534 174 1854 2472
Fragments arranged by size:
2472 1854 755 534 432 174
MslI CAynn'nnrTG
Cuts at: 763 3028 4210 4337 763
Size: 2265 1182 127 2647
Fragments arranged by size:
2647 2265 1182 127
MspA1I CmG'CkG
Cuts at: 298 944 1120 1626 1774 2065 2076 2199 2256
Size: 646 176 506 148 291 11 123 57
Cuts at: 2256 2371 2531 3198 3332 3646 4006 5019 5264
Size: 115 160 667 134 314 360 1013 245
Cuts at: 5264 298
Size: 1255
Fragments arranged by size:
1255 1013 667 646 506 360 314 291
245 176 160 148 134 123 115 57
11
NaeI GCC'GGC
Cuts at: 361 1551 2093 2191 4402 361
Size: 1190 542 98 2211 2180
Fragments arranged by size:
2211 2180 1190 542 98
NarI GG'CG_CC
Cuts at: 3652 3900 3652
Size: 248 5973
NcoI C'CATG_G
Cuts at: 758 1976 758
Size: 1218 5003
NgoMIV G'CCGG_C
Cuts at: 359 1549 2091 2189 4400 359
Size: 1190 542 98 2211 2180
Fragments arranged by size:
2211 2180 1190 542 98
NotI GC'GGCC_GC
Cuts at: 1257 1257
Size: 6221
NsiI A_TGCA'T
Cuts at: 3215 3215
Size: 6221
NspI r_CATG'y
Cuts at: 1458 1500 3217 3274 3774 4681 1458
Size: 42 1717 57 500 907 2998
Fragments arranged by size:
2998 1717 907 500 57 42
PasI CC'CwG_GG
Cuts at: 1720 1720
Size: 6221
PciI A'CATG_T
Cuts at: 1454 4677 1454
Size: 3223 2998
PflMI CCAn_nnn'nTGG
Cuts at: 804 1416 2202 804
Size: 612 786 4823
Fragments arranged by size:
4823 786 612
PfoI T'CCnGG_A
Cuts at: 971 1988 971
Size: 1017 5204
PmeI GTTT'AAAC
Cuts at: 3220 3220
Size: 6221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2905 2937 5360 5392 2905
Size: 32 2423 32 3734
Fragments arranged by size:
3734 2423 32 32
PpuMI rG'GwC_Cy
Cuts at: 405 1654 1880 2856 405
Size: 1249 226 976 3770
Fragments arranged by size:
3770 1249 976 226
PshAI GACnn'nnGTC
Cuts at: 2415 2415
Size: 6221
PspOMI G'GGCC_C
Cuts at: 702 2031 2619 2703 2793 702
Size: 1329 588 84 90 4130
Fragments arranged by size:
4130 1329 588 90 84
PspXI vC'TCGA_Gb
Cuts at: 823 1914 823
Size: 1091 5130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 760 792 2215 2247 760
Size: 32 1423 32 4734
Fragments arranged by size:
4734 1423 32 32
PstI C_TGCA'G
Cuts at: 297 887 1040 1355 1784 2070 2567 2873 3268
Size: 590 153 315 429 286 497 306 395
Cuts at: 3268 3953 297
Size: 685 2565
Fragments arranged by size:
2565 685 590 497 429 395 315 306
286 153
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6221
PvuII CAG'CTG
Cuts at: 298 1626 1774 2076 2199 3198 3646 4006 298
Size: 1328 148 302 123 999 448 360 2513
Fragments arranged by size:
2513 1328 999 448 360 302 148 123
RsrII CG'GwC_CG
Cuts at: 4416 4416
Size: 6221
SacI G_AGCT'C
Cuts at: 1586 2311 2840 3235 1586
Size: 725 529 395 4572
Fragments arranged by size:
4572 725 529 395
SacII CC_GC'GG
Cuts at: 2372 2532 2372
Size: 160 6061
SalI G'TCGA_C
Cuts at: 3258 3258
Size: 6221
SanDI GG'GwC_CC
Cuts at: 1654 1654
Size: 6221
SapI GCTCTTCn'nnn_
Cuts at: 4244 4454 4244
Size: 210 6011
SbfI CC_TGCA'GG
Cuts at: 1040 3268 1040
Size: 2228 3993
ScaI AGT'ACT
Cuts at: 5738 5738
Size: 6221
SfcI C'TryA_G
Cuts at: 33 293 883 1036 1351 1780 2066 2563 2869
Size: 260 590 153 315 429 286 497 306
Cuts at: 2869 3264 3949 4942 5133 33
Size: 395 685 993 191 1121
Fragments arranged by size:
1121 993 685 590 497 429 395 315
306 286 260 191 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 994 994
Size: 6221
SfoI GGC'GCC
Cuts at: 3653 3901 3653
Size: 248 5973
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
SmaI CCC'GGG
Cuts at: 2098 3243 2098
Size: 1145 5076
SmlI C'TyrA_G
Cuts at: 481 823 1012 1147 1914 2580 3376 4291 4783
Size: 342 189 135 767 666 796 915 492
Cuts at: 4783 5045 481
Size: 262 1657
Fragments arranged by size:
1657 915 796 767 666 492 342 262
189 135
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6221
SphI G_CATG'C
Cuts at: 3274 3774 3274
Size: 500 5721
SrfI GCCC'GGGC
Cuts at: 2098 2098
Size: 6221
StyI C'CwwG_G
Cuts at: 228 401 689 758 848 992 1047 1400 1976
Size: 173 288 69 90 144 55 353 576
Cuts at: 1976 2271 2657 3355 3709 228
Size: 295 386 698 354 2740
Fragments arranged by size:
2740 698 576 386 354 353 295 288
173 144 90 69 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 378 586 1423 2604 3804 3937 4396 4605 378
Size: 208 837 1181 1200 133 459 209 1994
Fragments arranged by size:
1994 1200 1181 837 459 209 208 133
TatI w'GTAC_w
Cuts at: 767 5736 767
Size: 4969 1252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5738 5770 5738
Size: 32 6189
Tth111I GACn'n_nGTC
Cuts at: 258 4018 258
Size: 3760 2461
XbaI T'CTAG_A
Cuts at: 3252 3252
Size: 6221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 162 756 801 855 2242 2898 3716 162
Size: 594 45 54 1387 656 818 2667
Fragments arranged by size:
2667 1387 818 656 594 54 45
XhoI C'TCGA_G
Cuts at: 823 1914 823
Size: 1091 5130
XmaI C'CCGG_G
Cuts at: 2096 3241 2096
Size: 1145 5076
XmnI GAAnn'nnTTC
Cuts at: 907 1471 907
Size: 564 5657
ZraI GAC'GTC
Cuts at: 260 1680 2040 260
Size: 1420 360 4441
Fragments arranged by size:
4441 1420 360
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AhdI AleI BaeI BmgBI BmtI BsiWI BsmI
BstZ17I ClaI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NruI PacI PmlI PsiI SexAI SgrAI SnaBI
SspI StuI SwaI