Restriction map for
pF1KE3496
(Circular) (Six-base) MAPSORT of: pF1KE3496.seq Check: 5221 from: 1 to: 9023
pF1KE3496 9023 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1433 1628 3248 4908 1433
Size: 195 1620 1660 5548
Fragments arranged by size:
5548 1660 1620 195
AatII G_ACGT'C
Cuts at: 1766 1766
Size: 9023
AccI GT'mk_AC
Cuts at: 539 1546 1810 3198 3692 5665 5810 6061 539
Size: 1007 264 1388 494 1973 145 251 3501
Fragments arranged by size:
3501 1973 1388 1007 494 264 251 145
Acc65I G'GTAC_C
Cuts at: 665 6039 665
Size: 5374 3649
AclI AA'CG_TT
Cuts at: 3601 6017 8863 3601
Size: 2416 2846 3761
Fragments arranged by size:
3761 2846 2416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1144 4761 6848 7280 8027 8295 220
Size: 924 3617 2087 432 747 268 948
Fragments arranged by size:
3617 2087 948 924 747 432 268
AflII C'TTAA_G
Cuts at: 2833 3478 2833
Size: 645 8378
AflIII A'CryG_T
Cuts at: 325 534 1454 6375 7479 8456 325
Size: 209 920 4921 1104 977 892
Fragments arranged by size:
4921 1104 977 920 892 209
AgeI A'CCGG_T
Cuts at: 7411 7411
Size: 9023
AhdI GACnn_n'nnGTC
Cuts at: 347 4360 347
Size: 4013 5010
AleI CACnn'nnGTG
Cuts at: 116 1057 1457 1738 1987 3661 116
Size: 941 400 281 249 1674 5478
Fragments arranged by size:
5478 1674 941 400 281 249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 929 1408 4560 929
Size: 479 3152 5392
Fragments arranged by size:
5392 3152 479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 580 612 669 701 580
Size: 32 57 32 8902
Fragments arranged by size:
8902 57 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1632 1659 2823 6328 7895 1632
Size: 27 1164 3505 1567 2760
Fragments arranged by size:
3505 2760 1567 1164 27
ApaI G_GGCC'C
Cuts at: 2895 2895
Size: 9023
ApaLI G'TGCA_C
Cuts at: 7793 7793
Size: 9023
ApoI r'AATT_y
Cuts at: 1747 2971 4087 6027 8376 8632 1747
Size: 1224 1116 1940 2349 256 2138
Fragments arranged by size:
2349 2138 1940 1224 1116 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 9023
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9023
AvaI C'yCGr_G
Cuts at: 129 285 2351 3355 3551 4821 6043 129
Size: 156 2066 1004 196 1270 1222 3109
Fragments arranged by size:
3109 2066 1270 1222 1004 196 156
BamHI G'GATC_C
Cuts at: 677 4109 6048 677
Size: 3432 1939 3652
Fragments arranged by size:
3652 3432 1939
BanI G'GyrC_C
Cuts at: 665 1351 4693 4867 6039 6453 6701 6736 665
Size: 686 3342 174 1172 414 248 35 2952
Fragments arranged by size:
3342 2952 1172 686 414 248 174 35
BanII G_rGCy'C
Cuts at: 827 913 2288 2648 2895 3375 3488 4819 4913
Size: 86 1375 360 247 480 113 1331 94
Cuts at: 4913 6037 7067 827
Size: 1124 1030 2783
Fragments arranged by size:
2783 1375 1331 1124 1030 480 360 247
113 94 86
BbeI G_GCGC'C
Cuts at: 1355 4697 6457 6705 1355
Size: 3342 1760 248 3673
Fragments arranged by size:
3673 3342 1760 248
BbsI GAAGACnn'nnnn_
Cuts at: 418 1758 1952 2792 3522 3948 418
Size: 1340 194 840 730 426 5493
Fragments arranged by size:
5493 1340 840 730 426 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 8989
BciVI GTATCCnnnnn_n'
Cuts at: 1924 2674 6921 7682 9023 1924
Size: 750 4247 761 1341 1924
Fragments arranged by size:
4247 1924 1341 761 750
BclI T'GATC_A
Cuts at: 2012 2699 6543 2012
Size: 687 3844 4492
Fragments arranged by size:
4492 3844 687
BglI GCCn_nnn'nGGC
Cuts at: 362 653 2798 362
Size: 291 2145 6587
Fragments arranged by size:
6587 2145 291
BglII A'GATC_T
Cuts at: 776 4199 6538 8575 776
Size: 3423 2339 2037 1224
Fragments arranged by size:
3423 2339 2037 1224
BlpI GC'TnA_GC
Cuts at: 2935 6135 2935
Size: 3200 5823
Bme1580I G_kGCm'C
Cuts at: 875 1250 2047 2895 3473 6648 6741 7797 875
Size: 375 797 848 578 3175 93 1056 2101
Fragments arranged by size:
3175 2101 1056 848 797 578 375 93
BmgBI CAC'GTC
Cuts at: 1814 2048 2931 4161 4181 4260 4419 1814
Size: 234 883 1230 20 79 159 6418
Fragments arranged by size:
6418 1230 883 234 159 79 20
BmrI ACTGGGnnnn_n'
Cuts at: 1406 4622 4700 4745 6235 6316 6651 8795 1406
Size: 3216 78 45 1490 81 335 2144 1634
Fragments arranged by size:
3216 2144 1634 1490 335 81 78 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4932 7390 4932
Size: 2458 6565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1654 1792 2302 3044 3610 4135 4261 5789
Size: 633 138 510 742 566 525 126 1528
Cuts at: 5789 1021
Size: 4255
Fragments arranged by size:
4255 1528 742 633 566 525 510 138
126
Bpu10I CC'TnA_GC
Cuts at: 3004 3004
Size: 9023
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 4824 6199 7078 7570 7868 1099
Size: 3725 1375 879 492 298 2254
Fragments arranged by size:
3725 2254 1375 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4473 4808 8727 4473
Size: 335 3919 4769
Fragments arranged by size:
4769 3919 335
BsaAI yAC'GTr
Cuts at: 396 6376 396
Size: 5980 3043
BsaBI GATnn'nnATC
Cuts at: 6 2446 4069 4345 6537 8574 6
Size: 2440 1623 276 2192 2037 455
Fragments arranged by size:
2440 2192 2037 1623 455 276
BsaHI Gr'CG_yC
Cuts at: 1107 1352 1763 4694 6454 6702 7404 8900 1107
Size: 245 411 2931 1760 248 702 1496 1230
Fragments arranged by size:
2931 1760 1496 1230 702 411 248 245
BsaWI w'CCGG_w
Cuts at: 189 2422 2519 3776 6212 6733 7411 7685 7832
Size: 2233 97 1257 2436 521 678 274 147
Cuts at: 7832 8243 189
Size: 411 969
Fragments arranged by size:
2436 2233 1257 969 678 521 411 274
147 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3310 3340 4249 4279 5913 5943 3310
Size: 30 909 30 1634 30 6390
Fragments arranged by size:
6390 1634 909 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 595 814 1024 2137 2358 3289 3934 6025 595
Size: 219 210 1113 221 931 645 2091 3593
Fragments arranged by size:
3593 2091 1113 931 645 221 219 210
BseYI C'CCAG_C
Cuts at: 813 3462 4212 4485 4879 5029 5050 5071 5092
Size: 2649 750 273 394 150 21 21 21
Cuts at: 5092 5113 5134 5197 5218 5239 5260 5302 5386
Size: 21 21 63 21 21 21 42 84
Cuts at: 5386 5407 5491 5575 5587 5596 5650 6448 7206
Size: 21 84 84 12 9 54 798 758
Cuts at: 7206 7783 8279 813
Size: 577 496 1557
Fragments arranged by size:
2649 1557 798 758 750 577 496 394
273 150 84 84 84 63 54 42
21 21 21 21 21 21 21 21
21 12 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 1483 1741 2788 3103 350
Size: 1133 258 1047 315 6270
Fragments arranged by size:
6270 1133 1047 315 258
BsiEI CG_ry'CG
Cuts at: 104 4505 6565 6611 7819 104
Size: 4401 2060 46 1208 1308
Fragments arranged by size:
4401 2060 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3005 3488 3658 3878 6037 6815 7005 7797 3005
Size: 483 170 220 2159 778 190 792 4231
Fragments arranged by size:
4231 2159 792 778 483 220 190 170
BsiWI C'GTAC_G
Cuts at: 4170 4170
Size: 9023
BsmI GAATG_Cn'
Cuts at: 1532 2336 3078 1532
Size: 804 742 7477
Fragments arranged by size:
7477 804 742
BsmBI CGTCTCn'nnnn_
Cuts at: 235 1630 1754 4111 5198 235
Size: 1395 124 2357 1087 4060
Fragments arranged by size:
4060 2357 1395 1087 124
Bsp1286I G_dGCh'C
Cuts at: 827 875 913 1250 2047 2288 2648 2895 3005
Size: 48 38 337 797 241 360 247 110
Cuts at: 3005 3375 3473 3488 3658 3878 4819 4913 6037
Size: 370 98 15 170 220 941 94 1124
Cuts at: 6037 6648 6741 6815 7005 7067 7797 827
Size: 611 93 74 190 62 730 2053
Fragments arranged by size:
2053 1124 941 797 730 611 370 360
337 247 241 220 190 170 110 98
94 93 74 62 48 38 15
BspEI T'CCGG_A
Cuts at: 189 2422 8243 189
Size: 2233 5821 969
Fragments arranged by size:
5821 2233 969
BspHI T'CATG_A
Cuts at: 2091 3890 4388 8199 2091
Size: 1799 498 3811 2915
Fragments arranged by size:
3811 2915 1799 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1433 1628 3062 3125 3248 3408 4908 6073 6287
Size: 195 1434 63 123 160 1500 1165 214
Cuts at: 6287 6589 6970 1433
Size: 302 381 3486
Fragments arranged by size:
3486 1500 1434 1165 381 302 214 195
160 123 63
BsrBI CCG'CTC
Cuts at: 50 306 879 1253 1776 2967 2979 3550 4245
Size: 256 573 374 523 1191 12 571 695
Cuts at: 4245 4916 7315 7369 8853 9018 50
Size: 671 2399 54 1484 165 55
Fragments arranged by size:
2399 1484 1191 695 671 573 571 523
374 256 165 55 54 12
BsrDI GCAATG_nn'
Cuts at: 990 1013 1256 2183 2459 3344 6935 990
Size: 23 243 927 276 885 3591 3078
Fragments arranged by size:
3591 3078 927 885 276 243 23
BsrFI r'CCGG_y
Cuts at: 1432 1484 1836 2593 4504 4603 4738 7021 7202
Size: 52 352 757 1911 99 135 2283 181
Cuts at: 7202 7411 1432
Size: 209 3044
Fragments arranged by size:
3044 2283 1911 757 352 209 181 135
99 52
BsrGI T'GTAC_A
Cuts at: 4028 4028
Size: 9023
BssSI C'ACGA_G
Cuts at: 7294 7652 7294
Size: 358 8665
BstAPI GCAn_nnn'nTGC
Cuts at: 4514 4684 6400 4514
Size: 170 1716 7137
Fragments arranged by size:
7137 1716 170
BstBI TT'CG_AA
Cuts at: 7384 7384
Size: 9023
BstXI CCAn_nnnn'nTGG
Cuts at: 1059 1992 3132 3436 4635 1059
Size: 933 1140 304 1199 5447
Fragments arranged by size:
5447 1199 1140 933 304
BstYI r'GATC_y
Cuts at: 1 627 677 746 776 1649 4109 4199 4562
Size: 626 50 69 30 873 2460 90 363
Cuts at: 4562 6048 6515 6538 6873 7119 8131 8217 8229
Size: 1486 467 23 335 246 1012 86 12
Cuts at: 8229 8575 1
Size: 346 449
Fragments arranged by size:
2460 1486 1012 873 626 467 449 363
346 335 246 90 86 69 50 30
23 12
BstZ17I GTA'TAC
Cuts at: 5666 5666
Size: 9023
Bsu36I CC'TnA_GG
Cuts at: 614 614
Size: 9023
BtgI C'CryG_G
Cuts at: 648 1055 1540 1664 2565 2627 2708 3788 4634
Size: 407 485 124 901 62 81 1080 846
Cuts at: 4634 648
Size: 5037
Fragments arranged by size:
5037 1080 901 846 485 407 124 81
62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3914 6973 7154 7317 3914
Size: 3059 181 163 5620
Fragments arranged by size:
5620 3059 181 163
BtsI GCAGTG_nn'
Cuts at: 1745 6266 1745
Size: 4521 4502
DraI TTT'AAA
Cuts at: 6022 6022
Size: 9023
DraIII CAC_nnn'GTG
Cuts at: 3254 4444 4864 3254
Size: 1190 420 7413
Fragments arranged by size:
7413 1190 420
DrdI GACnn_nn'nnGTC
Cuts at: 2659 3034 6729 7587 2659
Size: 375 3695 858 4095
Fragments arranged by size:
4095 3695 858 375
EaeI y'GGCC_r
Cuts at: 312 354 363 377 838 1049 1661 1838 4332
Size: 42 9 14 461 211 612 177 2494
Cuts at: 4332 4343 4502 6608 6782 7173 7200 8545 312
Size: 11 159 2106 174 391 27 1345 790
Fragments arranged by size:
2494 2106 1345 790 612 461 391 211
177 174 159 42 27 14 11 9
EagI C'GGCC_G
Cuts at: 4502 6608 4502
Size: 2106 6917
EarI CTCTTCn'nnn_
Cuts at: 2113 3232 3920 7046 7256 2113
Size: 1119 688 3126 210 3880
Fragments arranged by size:
3880 3126 1119 688 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7231 7541 7687 8833 7231
Size: 310 146 1146 7421
Fragments arranged by size:
7421 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3486 6035 3486
Size: 2549 6474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1021 1144 1654 1792 2302 3044 3610 4135
Size: 801 123 510 138 510 742 566 525
Cuts at: 4135 4261 4761 5789 6848 7280 8027 8295 220
Size: 126 500 1028 1059 432 747 268 948
Fragments arranged by size:
1059 1028 948 801 747 742 566 525
510 510 500 432 268 138 126 123
EcoNI CCTnn'n_nnAGG
Cuts at: 550 2152 2923 4859 550
Size: 1602 771 1936 4714
Fragments arranged by size:
4714 1936 1602 771
EcoO109I rG'GnC_Cy
Cuts at: 1827 2891 2892 2916 4095 4236 4826 4834 6162
Size: 1064 1 24 1179 141 590 8 1328
Cuts at: 6162 1827
Size: 4688
Fragments arranged by size:
4688 1328 1179 1064 590 141 24 8
1
EcoRI G'AATT_C
Cuts at: 4087 6027 4087
Size: 1940 7083
EcoRV GAT'ATC
Cuts at: 492 492
Size: 9023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1896 2886 4416 7042 1896
Size: 990 1530 2626 3877
Fragments arranged by size:
3877 2626 1530 990
FspI TGC'GCA
Cuts at: 154 1327 1744 3350 3383 6804 154
Size: 1173 417 1606 33 3421 2373
Fragments arranged by size:
3421 2373 1606 1173 417 33
HaeII r_GCGC'y
Cuts at: 1355 4697 6457 6705 7727 8706 1355
Size: 3342 1760 248 1022 979 1672
Fragments arranged by size:
3342 1760 1672 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 333 365 954 986 1506 1538 1634 1666 2514
Size: 32 589 32 520 32 96 32 848
Cuts at: 2514 2546 3310 3342 4346 4378 6865 6895 6897
Size: 32 764 32 1004 32 2487 30 2
Cuts at: 6897 6927 333
Size: 30 2429
Fragments arranged by size:
2487 2429 1004 848 764 589 520 96
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1811 3199 4365 6062 1811
Size: 1388 1166 1697 4772
Fragments arranged by size:
4772 1697 1388 1166
HindIII A'AGCT_T
Cuts at: 265 265
Size: 9023
KasI G'GCGC_C
Cuts at: 1351 4693 6453 6701 1351
Size: 3342 1760 248 3673
Fragments arranged by size:
3673 3342 1760 248
KpnI G_GTAC'C
Cuts at: 669 6043 669
Size: 5374 3649
MfeI C'AATT_G
Cuts at: 1908 1908
Size: 9023
MluI A'CGCG_T
Cuts at: 8456 8456
Size: 9023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2451 2867 2942 3137 4001 5023 5380 5506
Size: 310 416 75 195 864 1022 357 126
Cuts at: 5506 5578 7694 7878 2141
Size: 72 2116 184 3286
Fragments arranged by size:
3286 2116 1022 864 416 357 310 195
184 126 75 72
MscI TGG'CCA
Cuts at: 356 365 379 1051 1663 4334 4345 6784 356
Size: 9 14 672 612 2671 11 2439 2595
Fragments arranged by size:
2671 2595 2439 672 612 14 11 9
MslI CAynn'nnrTG
Cuts at: 116 722 1057 1457 1738 1937 1987 2273 3130
Size: 606 335 400 281 199 50 286 857
Cuts at: 3130 3409 3661 4606 7012 7139 116
Size: 279 252 945 2406 127 2000
Fragments arranged by size:
2406 2000 945 857 606 400 335 286
281 279 252 199 127 50
MspA1I CmG'CkG
Cuts at: 150 317 975 1003 1131 2053 2691 2772 4494
Size: 167 658 28 128 922 638 81 1722
Cuts at: 4494 4772 6134 6448 6808 7821 8066 150
Size: 278 1362 314 360 1013 245 1107
Fragments arranged by size:
1722 1362 1107 1013 922 658 638 360
314 278 245 167 128 81 28
NaeI GCC'GGC
Cuts at: 1838 2595 4506 4740 7204 1838
Size: 757 1911 234 2464 3657
Fragments arranged by size:
3657 2464 1911 757 234
NarI GG'CG_CC
Cuts at: 1352 4694 6454 6702 1352
Size: 3342 1760 248 3673
Fragments arranged by size:
3673 3342 1760 248
NcoI C'CATG_G
Cuts at: 648 1055 1540 1664 2627 2708 3788 4634 648
Size: 407 485 124 963 81 1080 846 5037
Fragments arranged by size:
5037 1080 963 846 485 407 124 81
NdeI CA'TA_TG
Cuts at: 565 565
Size: 9023
NgoMIV G'CCGG_C
Cuts at: 1836 2593 4504 4738 7202 1836
Size: 757 1911 234 2464 3657
Fragments arranged by size:
3657 2464 1911 757 234
NspI r_CATG'y
Cuts at: 146 329 538 1458 3984 4035 4611 6076 6576
Size: 183 209 920 2526 51 576 1465 500
Cuts at: 6576 7483 146
Size: 907 1686
Fragments arranged by size:
2526 1686 1465 920 907 576 500 209
183 51
PasI CC'CwG_GG
Cuts at: 2293 4729 2293
Size: 2436 6587
PciI A'CATG_T
Cuts at: 325 534 1454 7479 325
Size: 209 920 6025 1869
Fragments arranged by size:
6025 1869 920 209
PflMI CCAn_nnn'nTGG
Cuts at: 1659 2628 2823 4341 1659
Size: 969 195 1518 6341
Fragments arranged by size:
6341 1518 969 195
PfoI T'CCnGG_A
Cuts at: 1652 1769 4112 1652
Size: 117 2343 6563
Fragments arranged by size:
6563 2343 117
PmeI GTTT'AAAC
Cuts at: 6022 6022
Size: 9023
PmlI CAC'GTG
Cuts at: 396 396
Size: 9023
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2848 2880 3316 3348 8162 8194 2848
Size: 32 436 32 4814 32 3677
Fragments arranged by size:
4814 3677 436 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1827 2916 4826 4834 1827
Size: 1089 1910 8 6016
Fragments arranged by size:
6016 1910 1089 8
PspOMI G'GGCC_C
Cuts at: 2891 2891
Size: 9023
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1327 1359 1327
Size: 32 8991
PstI C_TGCA'G
Cuts at: 1430 2912 4004 4103 6070 6755 1430
Size: 1482 1092 99 1967 685 3698
Fragments arranged by size:
3698 1967 1482 1092 685 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9023
PvuII CAG'CTG
Cuts at: 975 2053 2772 4494 6448 6808 975
Size: 1078 719 1722 1954 360 3190
Fragments arranged by size:
3190 1954 1722 1078 719 360
RsrII CG'GwC_CG
Cuts at: 7218 7218
Size: 9023
SacI G_AGCT'C
Cuts at: 3488 6037 3488
Size: 2549 6474
SalI G'TCGA_C
Cuts at: 1809 3197 6060 1809
Size: 1388 2863 4772
Fragments arranged by size:
4772 2863 1388
SanDI GG'GwC_CC
Cuts at: 4826 4826
Size: 9023
SapI GCTCTTCn'nnn_
Cuts at: 3232 7046 7256 3232
Size: 3814 210 4999
Fragments arranged by size:
4999 3814 210
SbfI CC_TGCA'GG
Cuts at: 4004 4103 6070 4004
Size: 99 1967 6957
Fragments arranged by size:
6957 1967 99
ScaI AGT'ACT
Cuts at: 5686 5728 5833 5875 8540 5686
Size: 42 105 42 2665 6169
Fragments arranged by size:
6169 2665 105 42 42
SexAI A'CCwGG_T
Cuts at: 1235 1896 2759 2918 4860 1235
Size: 661 863 159 1942 5398
Fragments arranged by size:
5398 1942 863 661 159
SfcI C'TryA_G
Cuts at: 33 1426 2908 4000 4099 5013 5391 5454 5580
Size: 1393 1482 1092 99 914 378 63 126
Cuts at: 5580 5601 5967 6066 6751 7744 7935 33
Size: 21 366 99 685 993 191 1121
Fragments arranged by size:
1482 1393 1121 1092 993 914 685 378
366 191 126 99 99 63 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 362 362
Size: 9023
SfoI GGC'GCC
Cuts at: 1353 4695 6455 6703 1353
Size: 3342 1760 248 3673
Fragments arranged by size:
3673 3342 1760 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9023
SmaI CCC'GGG
Cuts at: 3357 4823 6045 3357
Size: 1466 1222 6335
Fragments arranged by size:
6335 1466 1222
SmlI C'TyrA_G
Cuts at: 1114 2833 3478 3551 4839 6178 7093 7585 7847
Size: 1719 645 73 1288 1339 915 492 262
Cuts at: 7847 1114
Size: 2290
Fragments arranged by size:
2290 1719 1339 1288 915 645 492 262
73
SpeI A'CTAG_T
Cuts at: 67 1320 5422 67
Size: 1253 4102 3668
Fragments arranged by size:
4102 3668 1253
SphI G_CATG'C
Cuts at: 146 6076 6576 146
Size: 5930 500 2593
Fragments arranged by size:
5930 2593 500
SspI AAT'ATT
Cuts at: 5707 5707
Size: 9023
StuI AGG'CCT
Cuts at: 3298 3298
Size: 9023
StyI C'CwwG_G
Cuts at: 506 648 1055 1540 1664 1985 2627 2708 3390
Size: 142 407 485 124 321 642 81 682
Cuts at: 3390 3569 3788 4634 4663 6157 6511 506
Size: 179 219 846 29 1494 354 3018
Fragments arranged by size:
3018 1494 846 682 642 485 407 354
321 219 179 142 124 81 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1945 2619 3421 3454 3672 4627 4641 5043 5064
Size: 674 802 33 218 955 14 402 21
Cuts at: 5064 5097 5160 5253 5307 5328 5349 5400 5421
Size: 33 63 93 54 21 21 51 21
Cuts at: 5421 5505 5589 5631 5727 5769 6606 6739 7198
Size: 84 84 42 96 42 837 133 459
Cuts at: 7198 7407 1945
Size: 209 3561
Fragments arranged by size:
3561 955 837 802 674 459 402 218
209 133 96 93 84 84 63 54
51 42 42 33 33 21 21 21
21 14
TatI w'GTAC_w
Cuts at: 1358 4028 5621 5684 5726 5831 5873 8538 1358
Size: 2670 1593 63 42 105 42 2665 1843
Fragments arranged by size:
2670 2665 1843 1593 105 63 42 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1492 1524 3229 3261 3565 3597 4249 4281 4659
Size: 32 1705 32 304 32 652 32 378
Cuts at: 4659 4691 8540 8572 1492
Size: 32 3849 32 1943
Fragments arranged by size:
3849 1943 1705 652 378 304 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6820 6820
Size: 9023
XbaI T'CTAG_A
Cuts at: 6054 6054
Size: 9023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 459 655 1992 2760 3132 4191 6518 459
Size: 196 1337 768 372 1059 2327 2964
Fragments arranged by size:
2964 2327 1337 1059 768 372 196
XhoI C'TCGA_G
Cuts at: 3551 3551
Size: 9023
XmaI C'CCGG_G
Cuts at: 3355 4821 6043 3355
Size: 1466 1222 6335
Fragments arranged by size:
6335 1466 1222
XmnI GAAnn'nnTTC
Cuts at: 2863 3085 3424 2863
Size: 222 339 8462
Fragments arranged by size:
8462 339 222
ZraI GAC'GTC
Cuts at: 1764 1764
Size: 9023
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BmtI BssHII
BstEII ClaI CspCI FseI FspAI HpaI NheI NotI
NruI NsiI PacI PshAI PsiI PspXI SacII SgrAI
SnaBI SrfI SwaI