Restriction map for
pF1KE3491
(Circular) (Six-base) MAPSORT of: pF1KE3491.seq Check: 7684 from: 1 to: 8708
pF1KE3491 8708 bp
With 182 enzymes: SgfI *
March 10, 2017 18:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2421 2421
Size: 8708
AatII G_ACGT'C
Cuts at: 1595 1595
Size: 8708
AccI GT'mk_AC
Cuts at: 1931 3344 5746 1931
Size: 1413 2402 4893
Fragments arranged by size:
4893 2402 1413
Acc65I G'GTAC_C
Cuts at: 5724 5724
Size: 8708
AclI AA'CG_TT
Cuts at: 1701 8548 1701
Size: 6847 1861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 1003 2117 2184 2266 2738 2849 4727 5508
Size: 524 1114 67 82 472 111 1878 781
Cuts at: 5508 5599 5615 5629 6533 6965 7712 7980 479
Size: 91 16 14 904 432 747 268 1207
Fragments arranged by size:
1878 1207 1114 904 781 747 524 472
432 268 111 91 82 67 16 14
AfeI AGC'GCT
Cuts at: 1957 1957
Size: 8708
AflII C'TTAA_G
Cuts at: 2272 2272
Size: 8708
AflIII A'CryG_T
Cuts at: 654 2684 6060 7164 8141 654
Size: 2030 3376 1104 977 1221
Fragments arranged by size:
3376 2030 1221 1104 977
AgeI A'CCGG_T
Cuts at: 7096 7096
Size: 8708
AleI CACnn'nnGTG
Cuts at: 643 643
Size: 8708
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4557 4589 5003 5035 4557
Size: 32 414 32 8230
Fragments arranged by size:
8230 414 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1410 4150 6013 7580 1410
Size: 2740 1863 1567 2538
Fragments arranged by size:
2740 2538 1863 1567
ApaLI G'TGCA_C
Cuts at: 7478 7478
Size: 8708
ApoI r'AATT_y
Cuts at: 209 393 541 929 1507 2373 2671 3670 3695
Size: 184 148 388 578 866 298 999 25
Cuts at: 3695 4132 4306 4414 5129 5284 5427 5655 5712
Size: 437 174 108 715 155 143 228 57
Cuts at: 5712 8061 8317 209
Size: 2349 256 600
Fragments arranged by size:
2349 999 866 715 600 578 437 388
298 256 228 184 174 155 148 143
108 57 25
AseI AT'TA_AT
Cuts at: 20 917 1711 2995 20
Size: 897 794 1284 5733
Fragments arranged by size:
5733 1284 897 794
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8708
AvaI C'yCGr_G
Cuts at: 5728 5728
Size: 8708
BamHI G'GATC_C
Cuts at: 1417 5733 1417
Size: 4316 4392
BanI G'GyrC_C
Cuts at: 4165 5724 6138 6386 6421 4165
Size: 1559 414 248 35 6452
Fragments arranged by size:
6452 1559 414 248 35
BanII G_rGCy'C
Cuts at: 370 1139 5722 6752 370
Size: 769 4583 1030 2326
Fragments arranged by size:
4583 2326 1030 769
BbeI G_GCGC'C
Cuts at: 6142 6390 6142
Size: 248 8460
BbsI GAAGACnn'nnnn_
Cuts at: 4631 4898 4631
Size: 267 8441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 686 720 686
Size: 34 8674
BciVI GTATCCnnnnn_n'
Cuts at: 4749 6606 7367 8708 4749
Size: 1857 761 1341 4749
Fragments arranged by size:
4749 1857 1341 761
BclI T'GATC_A
Cuts at: 6228 6228
Size: 8708
BfrBI ATG'CAT
Cuts at: 1321 1503 2962 3256 1321
Size: 182 1459 294 6773
Fragments arranged by size:
6773 1459 294 182
BglII A'GATC_T
Cuts at: 1074 2078 2816 3816 5574 6223 8260 1074
Size: 1004 738 1000 1758 649 2037 1522
Fragments arranged by size:
2037 1758 1522 1004 1000 738 649
BlpI GC'TnA_GC
Cuts at: 5820 5820
Size: 8708
Bme1580I G_kGCm'C
Cuts at: 6333 6426 7482 6333
Size: 93 1056 7559
Fragments arranged by size:
7559 1056 93
BmgBI CAC'GTC
Cuts at: 3317 3317
Size: 8708
BmrI ACTGGGnnnn_n'
Cuts at: 4077 5920 6001 6336 8480 4077
Size: 1843 81 335 2144 4305
Fragments arranged by size:
4305 2144 1843 335 81
BmtI G_CTAG'C
Cuts at: 1084 3881 5356 1084
Size: 2797 1475 4436
Fragments arranged by size:
4436 2797 1475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 685 7075 685
Size: 6390 2318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 647 1598 1746 2274 4178 5300 629
Size: 18 951 148 528 1904 1122 4037
Fragments arranged by size:
4037 1904 1122 951 528 148 18
Bpu10I CC'TnA_GC
Cuts at: 802 802
Size: 8708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2456 4001 5884 6763 7255 7553 2456
Size: 1545 1883 879 492 298 3611
Fragments arranged by size:
3611 1883 1545 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4732 8412 4732
Size: 3680 5028
BsaAI yAC'GTr
Cuts at: 6061 6061
Size: 8708
BsaBI GATnn'nnATC
Cuts at: 6 1268 2480 5639 6222 8259 6
Size: 1262 1212 3159 583 2037 455
Fragments arranged by size:
3159 2037 1262 1212 583 455
BsaHI Gr'CG_yC
Cuts at: 1592 6139 6387 7089 8585 1592
Size: 4547 248 702 1496 1715
Fragments arranged by size:
4547 1715 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4662 5897 6418 7096 7370 7517 7928 4662
Size: 1235 521 678 274 147 411 5442
Fragments arranged by size:
5442 1235 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3743 3773 4412 4442 3743
Size: 30 639 30 8009
Fragments arranged by size:
8009 639 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 644 1763 3485 3996 4972 4982 644
Size: 1119 1722 511 976 10 4370
Fragments arranged by size:
4370 1722 1119 976 511 10
BseYI C'CCAG_C
Cuts at: 2833 5490 6133 6891 7468 7964 2833
Size: 2657 643 758 577 496 3577
Fragments arranged by size:
3577 2657 758 643 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1283 1283
Size: 8708
BsiEI CG_ry'CG
Cuts at: 104 6250 6296 7504 104
Size: 6146 46 1208 1308
Fragments arranged by size:
6146 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 5722 6500 6690 7482 5722
Size: 778 190 792 6948
Fragments arranged by size:
6948 792 778 190
BsmI GAATG_Cn'
Cuts at: 831 3731 4814 831
Size: 2900 1083 4725
Fragments arranged by size:
4725 2900 1083
BsmBI CGTCTCn'nnnn_
Cuts at: 1583 1583
Size: 8708
Bsp1286I G_dGCh'C
Cuts at: 370 1139 5722 6333 6426 6500 6690 6752 7482
Size: 769 4583 611 93 74 190 62 730
Cuts at: 7482 370
Size: 1596
Fragments arranged by size:
4583 1596 769 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 7928 7928
Size: 8708
BspHI T'CATG_A
Cuts at: 1791 3180 3474 3618 4632 7884 1791
Size: 1389 294 144 1014 3252 2615
Fragments arranged by size:
3252 2615 1389 1014 294 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2001 2275 2421 4119 5758 5972 6274 6655 2001
Size: 274 146 1698 1639 214 302 381 4054
Fragments arranged by size:
4054 1698 1639 381 302 274 214 146
BsrBI CCG'CTC
Cuts at: 50 7000 7054 8538 8703 50
Size: 6950 54 1484 165 55
Fragments arranged by size:
6950 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1370 6620 1370
Size: 5250 3458
BsrFI r'CCGG_y
Cuts at: 6706 6887 7096 6706
Size: 181 209 8318
Fragments arranged by size:
8318 209 181
BsrGI T'GTAC_A
Cuts at: 522 2320 2733 3354 522
Size: 1798 413 621 5876
Fragments arranged by size:
5876 1798 621 413
BssSI C'ACGA_G
Cuts at: 3902 6979 7337 3902
Size: 3077 358 5273
Fragments arranged by size:
5273 3077 358
BstAPI GCAn_nnn'nTGC
Cuts at: 280 477 2068 6085 280
Size: 197 1591 4017 2903
Fragments arranged by size:
4017 2903 1591 197
BstBI TT'CG_AA
Cuts at: 7069 7069
Size: 8708
BstEII G'GTnAC_C
Cuts at: 310 310
Size: 8708
BstXI CCAn_nnnn'nTGG
Cuts at: 3927 3927
Size: 8708
BstYI r'GATC_y
Cuts at: 1 1074 1417 2078 2534 2816 3816 4077 5467
Size: 1073 343 661 456 282 1000 261 1390
Cuts at: 5467 5574 5733 6200 6223 6558 6804 7816 7902
Size: 107 159 467 23 335 246 1012 86
Cuts at: 7902 7914 8260 1
Size: 12 346 449
Fragments arranged by size:
1390 1073 1012 1000 661 467 456 449
346 343 335 282 261 246 159 107
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 5618 5618
Size: 8708
BtgI C'CryG_G
Cuts at: 724 2216 2540 724
Size: 1492 324 6892
Fragments arranged by size:
6892 1492 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6658 6839 7002 6658
Size: 181 163 8364
Fragments arranged by size:
8364 181 163
BtsI GCAGTG_nn'
Cuts at: 1300 1411 3640 3854 5951 1300
Size: 111 2229 214 2097 4057
Fragments arranged by size:
4057 2229 2097 214 111
DraI TTT'AAA
Cuts at: 2553 4464 5707 2553
Size: 1911 1243 5554
Fragments arranged by size:
5554 1911 1243
DrdI GACnn_nn'nnGTC
Cuts at: 6414 7272 6414
Size: 858 7850
EaeI y'GGCC_r
Cuts at: 945 1176 1749 2174 3098 3804 6293 6467 6858
Size: 231 573 425 924 706 2489 174 391
Cuts at: 6858 6885 8230 945
Size: 27 1345 1423
Fragments arranged by size:
2489 1423 1345 924 706 573 425 391
231 174 27
EagI C'GGCC_G
Cuts at: 6293 6293
Size: 8708
EarI CTCTTCn'nnn_
Cuts at: 891 1999 2525 3623 3683 4016 4164 4865 4974
Size: 1108 526 1098 60 333 148 701 109
Cuts at: 4974 5336 5575 6731 6941 891
Size: 362 239 1156 210 2658
Fragments arranged by size:
2658 1156 1108 1098 701 526 362 333
239 210 148 109 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 895 6916 7226 7372 8518 895
Size: 6021 310 146 1146 1085
Fragments arranged by size:
6021 1146 1085 310 146
EcoICRI GAG'CTC
Cuts at: 5720 5720
Size: 8708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 479 629 647 1003 1598 1746 2117 2184 2266
Size: 150 18 356 595 148 371 67 82
Cuts at: 2266 2274 2738 2849 4178 4727 5300 5508 5599
Size: 8 464 111 1329 549 573 208 91
Cuts at: 5599 5615 5629 6533 6965 7712 7980 479
Size: 16 14 904 432 747 268 1207
Fragments arranged by size:
1329 1207 904 747 595 573 549 464
432 371 356 268 208 150 148 111
91 82 67 18 16 14 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1692 4838 1692
Size: 3146 5562
EcoO109I rG'GnC_Cy
Cuts at: 180 3067 5847 180
Size: 2887 2780 3041
Fragments arranged by size:
3041 2887 2780
EcoRI G'AATT_C
Cuts at: 929 3695 5712 929
Size: 2766 2017 3925
Fragments arranged by size:
3925 2766 2017
EcoRV GAT'ATC
Cuts at: 5019 5019
Size: 8708
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1453 4042 6727 1453
Size: 2589 2685 3434
Fragments arranged by size:
3434 2685 2589
FspI TGC'GCA
Cuts at: 6489 6489
Size: 8708
HaeII r_GCGC'y
Cuts at: 1959 6142 6390 7412 8391 1959
Size: 4183 248 1022 979 2276
Fragments arranged by size:
4183 2276 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1610 1642 3114 3146 3743 3775 4669 4701 6550
Size: 32 1472 32 597 32 894 32 1849
Cuts at: 6550 6580 6582 6612 1610
Size: 30 2 30 3706
Fragments arranged by size:
3706 1849 1472 894 597 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1368 3323 3762 5747 1368
Size: 1955 439 1985 4329
Fragments arranged by size:
4329 1985 1955 439
HindIII A'AGCT_T
Cuts at: 2620 5155 5560 2620
Size: 2535 405 5768
Fragments arranged by size:
5768 2535 405
KasI G'GCGC_C
Cuts at: 6138 6386 6138
Size: 248 8460
KpnI G_GTAC'C
Cuts at: 5728 5728
Size: 8708
MfeI C'AATT_G
Cuts at: 2663 2894 2663
Size: 231 8477
MluI A'CGCG_T
Cuts at: 8141 8141
Size: 8708
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 639 846 1395 1582 3729 3851 5350 7379 7563
Size: 207 549 187 2147 122 1499 2029 184
Cuts at: 7563 639
Size: 1784
Fragments arranged by size:
2147 2029 1784 1499 549 207 187 184
122
MscI TGG'CCA
Cuts at: 947 1178 3100 3806 6469 947
Size: 231 1922 706 2663 3186
Fragments arranged by size:
3186 2663 1922 706 231
MslI CAynn'nnrTG
Cuts at: 643 847 1353 2221 2545 3118 6697 6824 643
Size: 204 506 868 324 573 3579 127 2527
Fragments arranged by size:
3579 2527 868 573 506 324 204 127
MspA1I CmG'CkG
Cuts at: 4777 5819 6133 6493 7506 7751 4777
Size: 1042 314 360 1013 245 5734
Fragments arranged by size:
5734 1042 1013 360 314 245
NaeI GCC'GGC
Cuts at: 6889 6889
Size: 8708
NarI GG'CG_CC
Cuts at: 6139 6387 6139
Size: 248 8460
NcoI C'CATG_G
Cuts at: 724 2216 2540 724
Size: 1492 324 6892
Fragments arranged by size:
6892 1492 324
NdeI CA'TA_TG
Cuts at: 444 2097 2964 3541 444
Size: 1653 867 577 5611
Fragments arranged by size:
5611 1653 867 577
NgoMIV G'CCGG_C
Cuts at: 6887 6887
Size: 8708
NheI G'CTAG_C
Cuts at: 1080 3877 5352 1080
Size: 2797 1475 4436
Fragments arranged by size:
4436 2797 1475
NsiI A_TGCA'T
Cuts at: 1323 1505 2964 3258 1323
Size: 182 1459 294 6773
Fragments arranged by size:
6773 1459 294 182
NspI r_CATG'y
Cuts at: 262 537 658 682 1503 4905 5761 6261 7168
Size: 275 121 24 821 3402 856 500 907
Cuts at: 7168 262
Size: 1802
Fragments arranged by size:
3402 1802 907 856 821 500 275 121
24
PciI A'CATG_T
Cuts at: 654 7164 654
Size: 6510 2198
PflMI CCAn_nnn'nTGG
Cuts at: 4150 4150
Size: 8708
PfoI T'CCnGG_A
Cuts at: 121 198 1614 121
Size: 77 1416 7215
Fragments arranged by size:
7215 1416 77
PmeI GTTT'AAAC
Cuts at: 5707 5707
Size: 8708
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7847 7879 7847
Size: 32 8676
PpuMI rG'GwC_Cy
Cuts at: 180 3067 180
Size: 2887 5821
PsiI TTA'TAA
Cuts at: 5093 5093
Size: 8708
PstI C_TGCA'G
Cuts at: 600 1074 4116 5755 6440 600
Size: 474 3042 1639 685 2868
Fragments arranged by size:
3042 2868 1639 685 474
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8708
PvuII CAG'CTG
Cuts at: 4777 6133 6493 4777
Size: 1356 360 6992
Fragments arranged by size:
6992 1356 360
RsrII CG'GwC_CG
Cuts at: 6903 6903
Size: 8708
SacI G_AGCT'C
Cuts at: 5722 5722
Size: 8708
SalI G'TCGA_C
Cuts at: 5745 5745
Size: 8708
SapI GCTCTTCn'nnn_
Cuts at: 3623 4974 6731 6941 3623
Size: 1351 1757 210 5390
Fragments arranged by size:
5390 1757 1351 210
SbfI CC_TGCA'GG
Cuts at: 5755 5755
Size: 8708
ScaI AGT'ACT
Cuts at: 8225 8225
Size: 8708
SexAI A'CCwGG_T
Cuts at: 2117 5002 2117
Size: 2885 5823
SfcI C'TryA_G
Cuts at: 33 417 596 1070 1677 4112 4394 5553 5751
Size: 384 179 474 607 2435 282 1159 198
Cuts at: 5751 6436 7429 7620 33
Size: 685 993 191 1121
Fragments arranged by size:
2435 1159 1121 993 685 607 474 384
282 198 191 179
SfoI GGC'GCC
Cuts at: 6140 6388 6140
Size: 248 8460
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8708
SmaI CCC'GGG
Cuts at: 5730 5730
Size: 8708
SmlI C'TyrA_G
Cuts at: 2272 2471 4016 5863 6778 7270 7532 2272
Size: 199 1545 1847 915 492 262 3448
Fragments arranged by size:
3448 1847 1545 915 492 262 199
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8708
SphI G_CATG'C
Cuts at: 537 1503 5761 6261 537
Size: 966 4258 500 2984
Fragments arranged by size:
4258 2984 966 500
SspI AAT'ATT
Cuts at: 3017 3432 4056 4238 3017
Size: 415 624 182 7487
Fragments arranged by size:
7487 624 415 182
StuI AGG'CCT
Cuts at: 4925 4925
Size: 8708
StyI C'CwwG_G
Cuts at: 176 724 1421 2216 2540 5206 5842 6196 176
Size: 548 697 795 324 2666 636 354 2688
Fragments arranged by size:
2688 2666 795 697 636 548 354 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 569 3239 4896 6291 6424 6883 7092 298
Size: 271 2670 1657 1395 133 459 209 1914
Fragments arranged by size:
2670 1914 1657 1395 459 271 209 133
TatI w'GTAC_w
Cuts at: 522 2320 2733 3354 4318 4992 8223 522
Size: 1798 413 621 964 674 3231 1007
Fragments arranged by size:
3231 1798 1007 964 674 621 413
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3080 3112 3712 3744 4552 4584 8225 8257 3080
Size: 32 600 32 808 32 3641 32 3531
Fragments arranged by size:
3641 3531 808 600 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6505 6505
Size: 8708
XbaI T'CTAG_A
Cuts at: 5739 5739
Size: 8708
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1524 4354 6203 1524
Size: 2830 1849 4029
Fragments arranged by size:
4029 2830 1849
XmaI C'CCGG_G
Cuts at: 5728 5728
Size: 8708
XmnI GAAnn'nnTTC
Cuts at: 464 1016 1190 464
Size: 552 174 7982
Fragments arranged by size:
7982 552 174
ZraI GAC'GTC
Cuts at: 1593 1593
Size: 8708
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AhdI AlfI ApaI AscI AvrII BaeI BbvCI BglI
BsiWI BssHII BstZ17I ClaI CspCI DraIII FseI FspAI
HpaI NotI NruI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI