Restriction map for
pF1KE3466
(Circular) (Six-base) MAPSORT of: pF1KE3466.seq Check: 5742 from: 1 to: 7283
pF1KE3466 7283 bp
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AccI GT'mk_AC
Cuts at: 473 3110 4321 473
Size: 2637 1211 3435
Fragments arranged by size:
3435 2637 1211
Acc65I G'GTAC_C
Cuts at: 4299 4299
Size: 7283
AclI AA'CG_TT
Cuts at: 7123 7123
Size: 7283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1042 2298 2729 2795 5108 5540 6287 6555
Size: 717 1256 431 66 2313 432 747 268
Cuts at: 6555 325
Size: 1053
Fragments arranged by size:
2313 1256 1053 747 717 432 431 268
66
AflIII A'CryG_T
Cuts at: 537 2194 3197 3791 4635 5739 6716 537
Size: 1657 1003 594 844 1104 977 1104
Fragments arranged by size:
1657 1104 1104 1003 977 844 594
AgeI A'CCGG_T
Cuts at: 677 5671 677
Size: 4994 2289
AhdI GACnn_n'nnGTC
Cuts at: 570 570
Size: 7283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 160 160
Size: 7283
AlwNI CAG_nnn'CTG
Cuts at: 950 1165 4588 6155 950
Size: 215 3423 1567 2078
Fragments arranged by size:
3423 2078 1567 215
ApaI G_GGCC'C
Cuts at: 1089 3326 1089
Size: 2237 5046
ApaLI G'TGCA_C
Cuts at: 1823 6053 1823
Size: 4230 3053
ApoI r'AATT_y
Cuts at: 711 1320 1539 1891 2163 2345 2409 2895 3419
Size: 609 219 352 272 182 64 486 524
Cuts at: 3419 3606 3751 4287 6636 6892 711
Size: 187 145 536 2349 256 1102
Fragments arranged by size:
2349 1102 609 536 524 486 352 272
256 219 187 182 145 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 7283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7283
AvaI C'yCGr_G
Cuts at: 2424 4303 2424
Size: 1879 5404
BamHI G'GATC_C
Cuts at: 2073 2582 4308 2073
Size: 509 1726 5048
Fragments arranged by size:
5048 1726 509
BanI G'GyrC_C
Cuts at: 301 1727 4067 4299 4713 4961 4996 301
Size: 1426 2340 232 414 248 35 2588
Fragments arranged by size:
2588 2340 1426 414 248 232 35
BanII G_rGCy'C
Cuts at: 614 1089 3326 3951 4297 5327 614
Size: 475 2237 625 346 1030 2570
Fragments arranged by size:
2570 2237 1030 625 475 346
BbeI G_GCGC'C
Cuts at: 4717 4965 4717
Size: 248 7035
BbsI GAAGACnn'nnnn_
Cuts at: 341 3655 4014 341
Size: 3314 359 3610
Fragments arranged by size:
3610 3314 359
BciVI GTATCCnnnnn_n'
Cuts at: 3194 4211 5181 5942 7283 3194
Size: 1017 970 761 1341 3194
Fragments arranged by size:
3194 1341 1017 970 761
BclI T'GATC_A
Cuts at: 489 4803 489
Size: 4314 2969
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 7283
BglII A'GATC_T
Cuts at: 1784 2994 3597 3645 4798 6835 1784
Size: 1210 603 48 1153 2037 2232
Fragments arranged by size:
2232 2037 1210 1153 603 48
BlpI GC'TnA_GC
Cuts at: 4395 4395
Size: 7283
Bme1580I G_kGCm'C
Cuts at: 1089 1827 3066 3326 4908 5001 6057 1089
Size: 738 1239 260 1582 93 1056 2315
Fragments arranged by size:
2315 1582 1239 1056 738 260 93
BmrI ACTGGGnnnn_n'
Cuts at: 374 1634 3320 4495 4576 4911 7055 374
Size: 1260 1686 1175 81 335 2144 602
Fragments arranged by size:
2144 1686 1260 1175 602 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5650 5650
Size: 7283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1249 1553 1015
Size: 234 304 6745
Fragments arranged by size:
6745 304 234
Bpu10I CC'TnA_GC
Cuts at: 1762 1762
Size: 7283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4459 5338 5830 6128 4459
Size: 879 492 298 5614
Fragments arranged by size:
5614 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1445 3566 6987 1445
Size: 2121 3421 1741
Fragments arranged by size:
3421 2121 1741
BsaAI yAC'GTr
Cuts at: 4636 4636
Size: 7283
BsaBI GATnn'nnATC
Cuts at: 6 1352 4797 6834 6
Size: 1346 3445 2037 455
Fragments arranged by size:
3445 2037 1346 455
BsaHI Gr'CG_yC
Cuts at: 4714 4962 5664 7160 4714
Size: 248 702 1496 4837
Fragments arranged by size:
4837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 677 4472 4993 5671 5945 6092 6503 677
Size: 3795 521 678 274 147 411 1457
Fragments arranged by size:
3795 1457 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 641 671 641
Size: 30 7253
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 3029 3913 3959 183
Size: 2846 884 46 3507
Fragments arranged by size:
3507 2846 884 46
BseYI C'CCAG_C
Cuts at: 613 1088 1862 2241 4708 5466 6043 6539 613
Size: 475 774 379 2467 758 577 496 1357
Fragments arranged by size:
2467 1357 774 758 577 496 475 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1920 3199 3325 184
Size: 1736 1279 126 4142
Fragments arranged by size:
4142 1736 1279 126
BsiEI CG_ry'CG
Cuts at: 104 1750 4825 4871 6079 104
Size: 1646 3075 46 1208 1308
Fragments arranged by size:
3075 1646 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 1295 1827 1960 2383 3279 3923 4151 4297
Size: 1146 532 133 423 896 644 228 146
Cuts at: 4297 5075 5265 6057 149
Size: 778 190 792 1375
Fragments arranged by size:
1375 1146 896 792 778 644 532 423
228 190 146 133
BsmI GAATG_Cn'
Cuts at: 741 972 1198 1762 741
Size: 231 226 564 6262
Fragments arranged by size:
6262 564 231 226
Bsp1286I G_dGCh'C
Cuts at: 149 614 1089 1295 1827 1960 2383 3066 3279
Size: 465 475 206 532 133 423 683 213
Cuts at: 3279 3326 3923 3951 4151 4297 4908 5001 5075
Size: 47 597 28 200 146 611 93 74
Cuts at: 5075 5265 5327 6057 149
Size: 190 62 730 1375
Fragments arranged by size:
1375 730 683 611 597 532 475 465
423 213 206 200 190 146 133 93
74 62 47 28
BspEI T'CCGG_A
Cuts at: 6503 6503
Size: 7283
BspHI T'CATG_A
Cuts at: 1287 3497 6459 1287
Size: 2210 2962 2111
Fragments arranged by size:
2962 2210 2111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 535 1488 2813 4333 4547 4849 5230 535
Size: 953 1325 1520 214 302 381 2588
Fragments arranged by size:
2588 1520 1325 953 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1766 5575 5629 7113 7278 50
Size: 1716 3809 54 1484 165 55
Fragments arranged by size:
3809 1716 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 180 736 2673 5195 180
Size: 556 1937 2522 2268
Fragments arranged by size:
2522 2268 1937 556
BsrFI r'CCGG_y
Cuts at: 677 5281 5462 5671 677
Size: 4604 181 209 2289
Fragments arranged by size:
4604 2289 209 181
BsrGI T'GTAC_A
Cuts at: 2613 3816 2613
Size: 1203 6080
BssHII G'CGCG_C
Cuts at: 1339 1339
Size: 7283
BssSI C'ACGA_G
Cuts at: 142 5554 5912 142
Size: 5412 358 1513
Fragments arranged by size:
5412 1513 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1691 4660 1691
Size: 2969 4314
BstBI TT'CG_AA
Cuts at: 3603 3615 5644 3603
Size: 12 2029 5242
Fragments arranged by size:
5242 2029 12
BstXI CCAn_nnnn'nTGG
Cuts at: 3851 3851
Size: 7283
BstYI r'GATC_y
Cuts at: 1 1027 1643 1784 2073 2328 2582 2994 3597
Size: 1026 616 141 289 255 254 412 603
Cuts at: 3597 3645 4308 4775 4798 5133 5379 6391 6477
Size: 48 663 467 23 335 246 1012 86
Cuts at: 6477 6489 6835 1
Size: 12 346 449
Fragments arranged by size:
1026 1012 663 616 603 467 449 412
346 335 289 255 254 246 141 86
48 23 12
BtgI C'CryG_G
Cuts at: 248 2037 248
Size: 1789 5494
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2467 5233 5414 5577 2467
Size: 2766 181 163 4173
Fragments arranged by size:
4173 2766 181 163
BtsI GCAGTG_nn'
Cuts at: 582 1293 3009 3308 4526 582
Size: 711 1716 299 1218 3339
Fragments arranged by size:
3339 1716 1218 711 299
ClaI AT'CG_AT
Cuts at: 1182 1182
Size: 7283
DraI TTT'AAA
Cuts at: 326 1901 2204 4282 326
Size: 1575 303 2078 3327
Fragments arranged by size:
3327 2078 1575 303
DrdI GACnn_nn'nnGTC
Cuts at: 801 3198 4989 5847 801
Size: 2397 1791 858 2237
Fragments arranged by size:
2397 2237 1791 858
EaeI y'GGCC_r
Cuts at: 2034 4868 5042 5433 5460 6805 2034
Size: 2834 174 391 27 1345 2512
Fragments arranged by size:
2834 2512 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4868 4868
Size: 7283
EarI CTCTTCn'nnn_
Cuts at: 602 3357 5306 5516 602
Size: 2755 1949 210 2369
Fragments arranged by size:
2755 2369 1949 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5491 5801 5947 7093 5491
Size: 310 146 1146 5681
Fragments arranged by size:
5681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4295 4295
Size: 7283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1015 1042 1249 1553 2298 2729 2795 5108
Size: 690 27 207 304 745 431 66 2313
Cuts at: 5108 5540 6287 6555 325
Size: 432 747 268 1053
Fragments arranged by size:
2313 1053 747 745 690 432 431 304
268 207 66 27
EcoNI CCTnn'n_nnAGG
Cuts at: 343 343
Size: 7283
EcoO109I rG'GnC_Cy
Cuts at: 114 524 1085 4422 114
Size: 410 561 3337 2975
Fragments arranged by size:
3337 2975 561 410
EcoRI G'AATT_C
Cuts at: 2163 2409 4287 2163
Size: 246 1878 5159
Fragments arranged by size:
5159 1878 246
EcoRV GAT'ATC
Cuts at: 749 4118 749
Size: 3369 3914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5302 5302
Size: 7283
FspI TGC'GCA
Cuts at: 5064 5064
Size: 7283
HaeII r_GCGC'y
Cuts at: 644 4717 4965 5987 6966 644
Size: 4073 248 1022 979 961
Fragments arranged by size:
4073 1022 979 961 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 641 673 1761 1793 3615 3647 3892
Size: 32 53 32 1088 32 1822 32 245
Cuts at: 3892 3924 5125 5155 5157 5187 556
Size: 32 1201 30 2 30 2652
Fragments arranged by size:
2652 1822 1201 1088 245 53 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 474 516 1218 1242 1389 2479 4322 474
Size: 42 702 24 147 1090 1843 3435
Fragments arranged by size:
3435 1843 1090 702 147 42 24
KasI G'GCGC_C
Cuts at: 4713 4961 4713
Size: 248 7035
KpnI G_GTAC'C
Cuts at: 4303 4303
Size: 7283
MfeI C'AATT_G
Cuts at: 2180 2180
Size: 7283
MluI A'CGCG_T
Cuts at: 6716 6716
Size: 7283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 554 2377 2718 3380 3658 5954 6138 554
Size: 1823 341 662 278 2296 184 1699
Fragments arranged by size:
2296 1823 1699 662 341 278 184
MscI TGG'CCA
Cuts at: 2036 5044 2036
Size: 3008 4275
MslI CAynn'nnrTG
Cuts at: 142 457 985 1974 2440 3347 3518 5272 5399
Size: 315 528 989 466 907 171 1754 127
Cuts at: 5399 142
Size: 2026
Fragments arranged by size:
2026 1754 989 907 528 466 315 171
127
MspA1I CmG'CkG
Cuts at: 121 250 2532 3567 4394 4708 5068 6081 6326
Size: 129 2282 1035 827 314 360 1013 245
Cuts at: 6326 121
Size: 1078
Fragments arranged by size:
2282 1078 1035 1013 827 360 314 245
129
NaeI GCC'GGC
Cuts at: 5464 5464
Size: 7283
NarI GG'CG_CC
Cuts at: 4714 4962 4714
Size: 248 7035
NdeI CA'TA_TG
Cuts at: 1855 1855
Size: 7283
NgoMIV G'CCGG_C
Cuts at: 5462 5462
Size: 7283
NspI r_CATG'y
Cuts at: 541 2198 2477 3201 3313 3795 4336 4836 5743
Size: 1657 279 724 112 482 541 500 907
Cuts at: 5743 541
Size: 2081
Fragments arranged by size:
2081 1657 907 724 541 500 482 279
112
PasI CC'CwG_GG
Cuts at: 645 645
Size: 7283
PciI A'CATG_T
Cuts at: 537 2194 3197 3791 5739 537
Size: 1657 1003 594 1948 2081
Fragments arranged by size:
2081 1948 1657 1003 594
PflMI CCAn_nnn'nTGG
Cuts at: 1654 1654
Size: 7283
PfoI T'CCnGG_A
Cuts at: 1547 4111 1547
Size: 2564 4719
PmeI GTTT'AAAC
Cuts at: 4282 4282
Size: 7283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6422 6454 6422
Size: 32 7251
PpuMI rG'GwC_Cy
Cuts at: 524 524
Size: 7283
PspOMI G'GGCC_C
Cuts at: 1085 3322 1085
Size: 2237 5046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1350 1382 1350
Size: 32 7251
PstI C_TGCA'G
Cuts at: 412 1669 1687 4330 5015 412
Size: 1257 18 2643 685 2680
Fragments arranged by size:
2680 2643 1257 685 18
PvuI CG_AT'CG
Cuts at: 104 1750 104
Size: 1646 5637
PvuII CAG'CTG
Cuts at: 2532 4708 5068 2532
Size: 2176 360 4747
Fragments arranged by size:
4747 2176 360
RsrII CG'GwC_CG
Cuts at: 5478 5478
Size: 7283
SacI G_AGCT'C
Cuts at: 4297 4297
Size: 7283
SacII CC_GC'GG
Cuts at: 251 251
Size: 7283
SalI G'TCGA_C
Cuts at: 472 4320 472
Size: 3848 3435
SapI GCTCTTCn'nnn_
Cuts at: 602 5306 5516 602
Size: 4704 210 2369
Fragments arranged by size:
4704 2369 210
SbfI CC_TGCA'GG
Cuts at: 4330 4330
Size: 7283
ScaI AGT'ACT
Cuts at: 6800 6800
Size: 7283
SfcI C'TryA_G
Cuts at: 33 408 1665 1683 3083 3107 3810 4326 5011
Size: 375 1257 18 1400 24 703 516 685
Cuts at: 5011 6004 6195 33
Size: 993 191 1121
Fragments arranged by size:
1400 1257 1121 993 703 685 516 375
191 24 18
SfoI GGC'GCC
Cuts at: 4715 4963 4715
Size: 248 7035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7283
SmaI CCC'GGG
Cuts at: 4305 4305
Size: 7283
SmlI C'TyrA_G
Cuts at: 4438 5353 5845 6107 4438
Size: 915 492 262 5614
Fragments arranged by size:
5614 915 492 262
SpeI A'CTAG_T
Cuts at: 67 3214 67
Size: 3147 4136
SphI G_CATG'C
Cuts at: 4336 4836 4336
Size: 500 6783
StyI C'CwwG_G
Cuts at: 158 297 1920 2865 4417 4771 158
Size: 139 1623 945 1552 354 2670
Fragments arranged by size:
2670 1623 1552 945 354 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 273 398 3499 4866 4999 5458 5667 273
Size: 125 3101 1367 133 459 209 1889
Fragments arranged by size:
3101 1889 1367 459 209 133 125
TatI w'GTAC_w
Cuts at: 356 2056 2197 2613 2676 3030 3816 6798 356
Size: 1700 141 416 63 354 786 2982 841
Fragments arranged by size:
2982 1700 841 786 416 354 141 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 1971 2003 6800 6832 122
Size: 32 1817 32 4797 32 573
Fragments arranged by size:
4797 1817 573 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5080 5080
Size: 7283
XbaI T'CTAG_A
Cuts at: 912 2167 4314 912
Size: 1255 2147 3881
Fragments arranged by size:
3881 2147 1255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4262 4778 4262
Size: 516 6767
XmaI C'CCGG_G
Cuts at: 4303 4303
Size: 7283
XmnI GAAnn'nnTTC
Cuts at: 338 3011 338
Size: 2673 4610
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BstEII BstZ17I Bsu36I CspCI DraIII FseI FspAI HindIII
HpaI NcoI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI