Restriction map for
pF1KE3380
(Circular) (Six-base) MAPSORT of: pF1KE3380.seq Check: 6726 from: 1 to: 6509
pF1KE3380 6509 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 3547 3547
Size: 6509
Acc65I G'GTAC_C
Cuts at: 2262 2326 3525 2262
Size: 64 1199 5246
Fragments arranged by size:
5246 1199 64
AclI AA'CG_TT
Cuts at: 6349 6349
Size: 6509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 548 929 1366 1503 1626 2227 2743 2792
Size: 340 381 437 137 123 601 516 49
Cuts at: 2792 4334 4766 5513 5781 208
Size: 1542 432 747 268 936
Fragments arranged by size:
1542 936 747 601 516 437 432 381
340 268 137 123 49
AflII C'TTAA_G
Cuts at: 862 862
Size: 6509
AflIII A'CryG_T
Cuts at: 2567 3861 4965 5942 2567
Size: 1294 1104 977 3134
Fragments arranged by size:
3134 1294 1104 977
AgeI A'CCGG_T
Cuts at: 4897 4897
Size: 6509
AleI CACnn'nnGTG
Cuts at: 343 2433 343
Size: 2090 4419
AlwNI CAG_nnn'CTG
Cuts at: 3814 5381 3814
Size: 1567 4942
ApaLI G'TGCA_C
Cuts at: 5279 5279
Size: 6509
ApoI r'AATT_y
Cuts at: 913 992 1996 2292 2298 2656 3286 3513 5862
Size: 79 1004 296 6 358 630 227 2349
Cuts at: 5862 6118 913
Size: 256 1304
Fragments arranged by size:
2349 1304 1004 630 358 296 256 227
79 6
AseI AT'TA_AT
Cuts at: 20 1210 2887 20
Size: 1190 1677 3642
Fragments arranged by size:
3642 1677 1190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
AvaI C'yCGr_G
Cuts at: 518 3529 518
Size: 3011 3498
AvrII C'CTAG_G
Cuts at: 2595 2595
Size: 6509
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 331 364 850 883 331
Size: 33 486 33 5957
Fragments arranged by size:
5957 486 33 33
BamHI G'GATC_C
Cuts at: 2625 3534 2625
Size: 909 5600
BanI G'GyrC_C
Cuts at: 2262 2326 2590 2608 3525 3939 4187 4222 2262
Size: 64 264 18 917 414 248 35 4549
Fragments arranged by size:
4549 917 414 264 248 64 35 18
BanII G_rGCy'C
Cuts at: 3523 4553 3523
Size: 1030 5479
BbeI G_GCGC'C
Cuts at: 3943 4191 3943
Size: 248 6261
BbsI GAAGACnn'nnnn_
Cuts at: 402 1632 2168 2176 2624 402
Size: 1230 536 8 448 4287
Fragments arranged by size:
4287 1230 536 448 8
BciVI GTATCCnnnnn_n'
Cuts at: 404 4407 5168 6509 404
Size: 4003 761 1341 404
Fragments arranged by size:
4003 1341 761 404
BclI T'GATC_A
Cuts at: 3407 4029 3407
Size: 622 5887
BfrBI ATG'CAT
Cuts at: 2091 2276 2091
Size: 185 6324
BglII A'GATC_T
Cuts at: 1533 2517 3398 4024 6061 1533
Size: 984 881 626 2037 1981
Fragments arranged by size:
2037 1981 984 881 626
BlpI GC'TnA_GC
Cuts at: 3621 3621
Size: 6509
Bme1580I G_kGCm'C
Cuts at: 2595 4134 4227 5283 2595
Size: 1539 93 1056 3821
Fragments arranged by size:
3821 1539 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2741 3721 3802 4137 6281 2741
Size: 980 81 335 2144 2969
Fragments arranged by size:
2969 2144 980 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1712 4876 1712
Size: 3164 3345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1259 2522 3158 3521 1259
Size: 1263 636 363 4247
Fragments arranged by size:
4247 1263 636 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4564 5056 5354 3685
Size: 879 492 298 4840
Fragments arranged by size:
4840 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6213 6213
Size: 6509
BsaAI yAC'GTr
Cuts at: 3862 3862
Size: 6509
BsaBI GATnn'nnATC
Cuts at: 6 1244 2015 2414 4023 6060 6
Size: 1238 771 399 1609 2037 455
Fragments arranged by size:
2037 1609 1238 771 455 399
BsaHI Gr'CG_yC
Cuts at: 3940 4188 4890 6386 3940
Size: 248 702 1496 4063
Fragments arranged by size:
4063 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3698 4219 4897 5171 5318 5729 3698
Size: 521 678 274 147 411 4478
Fragments arranged by size:
4478 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1940 1970 2310 2340 1940
Size: 30 340 30 6109
Fragments arranged by size:
6109 340 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 524 967 2369 524
Size: 443 1402 4664
Fragments arranged by size:
4664 1402 443
BseYI C'CCAG_C
Cuts at: 3934 4692 5269 5765 3934
Size: 758 577 496 4678
Fragments arranged by size:
4678 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2411
Size: 6509
BsiEI CG_ry'CG
Cuts at: 104 4051 4097 5305 104
Size: 3947 46 1208 1308
Fragments arranged by size:
3947 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2376 2780 3523 4301 4491 5283 2376
Size: 404 743 778 190 792 3602
Fragments arranged by size:
3602 792 778 743 404 190
BsiWI C'GTAC_G
Cuts at: 1794 1794
Size: 6509
BsmI GAATG_Cn'
Cuts at: 2278 3424 2278
Size: 1146 5363
BsmBI CGTCTCn'nnnn_
Cuts at: 1958 1958
Size: 6509
Bsp1286I G_dGCh'C
Cuts at: 2376 2595 2780 3523 4134 4227 4301 4491 4553
Size: 219 185 743 611 93 74 190 62
Cuts at: 4553 5283 2376
Size: 730 3602
Fragments arranged by size:
3602 743 730 611 219 190 185 93
74 62
BspEI T'CCGG_A
Cuts at: 5729 5729
Size: 6509
BspHI T'CATG_A
Cuts at: 621 3410 5685 621
Size: 2789 2275 1445
Fragments arranged by size:
2789 2275 1445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1345 3559 3773 4075 4456 1345
Size: 2214 214 302 381 3398
Fragments arranged by size:
3398 2214 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4801 4855 6339 6504 50
Size: 4751 54 1484 165 55
Fragments arranged by size:
4751 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3312 4421 3312
Size: 1109 5400
BsrFI r'CCGG_y
Cuts at: 2705 4507 4688 4897 2705
Size: 1802 181 209 4317
Fragments arranged by size:
4317 1802 209 181
BssSI C'ACGA_G
Cuts at: 4780 5138 4780
Size: 358 6151
BstAPI GCAn_nnn'nTGC
Cuts at: 3886 3886
Size: 6509
BstBI TT'CG_AA
Cuts at: 4870 4870
Size: 6509
BstXI CCAn_nnnn'nTGG
Cuts at: 114 247 114
Size: 133 6376
BstYI r'GATC_y
Cuts at: 1 213 386 758 1533 2517 2625 3033 3398
Size: 212 173 372 775 984 108 408 365
Cuts at: 3398 3459 3534 4001 4024 4359 4605 5617 5703
Size: 61 75 467 23 335 246 1012 86
Cuts at: 5703 5715 6061 1
Size: 12 346 449
Fragments arranged by size:
1012 984 775 467 449 408 372 365
346 335 246 212 173 108 86 75
61 23 12
Bsu36I CC'TnA_GG
Cuts at: 1394 1394
Size: 6509
BtgI C'CryG_G
Cuts at: 107 2257 107
Size: 2150 4359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4459 4640 4803 4459
Size: 181 163 6165
Fragments arranged by size:
6165 181 163
BtsI GCAGTG_nn'
Cuts at: 489 659 842 2355 3752 489
Size: 170 183 1513 1397 3246
Fragments arranged by size:
3246 1513 1397 183 170
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1482 1517 1482
Size: 35 6474
DraI TTT'AAA
Cuts at: 1788 1847 1940 2297 3508 1788
Size: 59 93 357 1211 4789
Fragments arranged by size:
4789 1211 357 93 59
DraIII CAC_nnn'GTG
Cuts at: 2336 2336
Size: 6509
DrdI GACnn_nn'nnGTC
Cuts at: 4215 5073 4215
Size: 858 5651
EaeI y'GGCC_r
Cuts at: 701 1761 4094 4268 4659 4686 6031 701
Size: 1060 2333 174 391 27 1345 1179
Fragments arranged by size:
2333 1345 1179 1060 391 174 27
EagI C'GGCC_G
Cuts at: 4094 4094
Size: 6509
EarI CTCTTCn'nnn_
Cuts at: 162 542 1481 1794 2636 2768 3259 3473 4532
Size: 380 939 313 842 132 491 214 1059
Cuts at: 4532 4742 162
Size: 210 1929
Fragments arranged by size:
1929 1059 939 842 491 380 313 214
210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4717 5027 5173 6319 4717
Size: 310 146 1146 4907
Fragments arranged by size:
4907 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3521 3521
Size: 6509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 548 929 1259 1366 1503 1626 2227 2522
Size: 340 381 330 107 137 123 601 295
Cuts at: 2522 2743 2792 3158 3521 4334 4766 5513 5781
Size: 221 49 366 363 813 432 747 268
Cuts at: 5781 208
Size: 936
Fragments arranged by size:
936 813 747 601 432 381 366 363
340 330 295 268 221 137 123 107
49
EcoNI CCTnn'n_nnAGG
Cuts at: 3453 3453
Size: 6509
EcoO109I rG'GnC_Cy
Cuts at: 3648 3648
Size: 6509
EcoRI G'AATT_C
Cuts at: 992 3513 992
Size: 2521 3988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 553 658 750 959 1208 1533 4528 553
Size: 105 92 209 249 325 2995 2534
Fragments arranged by size:
2995 2534 325 249 209 105 92
FspI TGC'GCA
Cuts at: 4290 4290
Size: 6509
HaeII r_GCGC'y
Cuts at: 3943 4191 5213 6192 3943
Size: 248 1022 979 4260
Fragments arranged by size:
4260 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 378 410 743 775 1427 1459 1940
Size: 32 213 32 333 32 652 32 481
Cuts at: 1940 1972 2163 2195 3258 3290 3311 3343 4351
Size: 32 191 32 1063 32 21 32 1008
Cuts at: 4351 4381 4383 4413 133
Size: 30 2 30 2229
Fragments arranged by size:
2229 1063 1008 652 481 333 213 191
32 32 32 32 32 32 32 32
30 30 21 2
HincII GTy'rAC
Cuts at: 428 1822 3098 3548 428
Size: 1394 1276 450 3389
Fragments arranged by size:
3389 1394 1276 450
HindIII A'AGCT_T
Cuts at: 326 1190 1915 2755 326
Size: 864 725 840 4080
Fragments arranged by size:
4080 864 840 725
HpaI GTT'AAC
Cuts at: 3098 3098
Size: 6509
KasI G'GCGC_C
Cuts at: 3939 4187 3939
Size: 248 6261
KpnI G_GTAC'C
Cuts at: 2266 2330 3529 2266
Size: 64 1199 5246
Fragments arranged by size:
5246 1199 64
MluI A'CGCG_T
Cuts at: 5942 5942
Size: 6509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5180 5364 5180
Size: 184 6325
MscI TGG'CCA
Cuts at: 703 1763 4270 703
Size: 1060 2507 2942
Fragments arranged by size:
2942 2507 1060
MslI CAynn'nnrTG
Cuts at: 112 343 419 2123 2271 2433 4498 4625 112
Size: 231 76 1704 148 162 2065 127 1996
Fragments arranged by size:
2065 1996 1704 231 162 148 127 76
MspA1I CmG'CkG
Cuts at: 3283 3620 3934 4294 5307 5552 3283
Size: 337 314 360 1013 245 4240
Fragments arranged by size:
4240 1013 360 337 314 245
NaeI GCC'GGC
Cuts at: 4690 4690
Size: 6509
NarI GG'CG_CC
Cuts at: 3940 4188 3940
Size: 248 6261
NcoI C'CATG_G
Cuts at: 107 2257 107
Size: 2150 4359
NdeI CA'TA_TG
Cuts at: 1700 2907 1700
Size: 1207 5302
NgoMIV G'CCGG_C
Cuts at: 4688 4688
Size: 6509
NsiI A_TGCA'T
Cuts at: 2093 2278 2093
Size: 185 6324
NspI r_CATG'y
Cuts at: 2571 3562 4062 4969 2571
Size: 991 500 907 4111
Fragments arranged by size:
4111 991 907 500
PciI A'CATG_T
Cuts at: 2567 4965 2567
Size: 2398 4111
PflMI CCAn_nnn'nTGG
Cuts at: 2336 3164 2336
Size: 828 5681
PmeI GTTT'AAAC
Cuts at: 3508 3508
Size: 6509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5648 5680 5648
Size: 32 6477
PshAI GACnn'nnGTC
Cuts at: 1817 1817
Size: 6509
PsiI TTA'TAA
Cuts at: 874 973 2458 874
Size: 99 1485 4925
Fragments arranged by size:
4925 1485 99
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1031 1063 1031
Size: 32 6477
PstI C_TGCA'G
Cuts at: 893 1239 2350 2972 3556 4241 893
Size: 346 1111 622 584 685 3161
Fragments arranged by size:
3161 1111 685 622 584 346
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6509
PvuII CAG'CTG
Cuts at: 3934 4294 3934
Size: 360 6149
RsrII CG'GwC_CG
Cuts at: 4704 4704
Size: 6509
SacI G_AGCT'C
Cuts at: 3523 3523
Size: 6509
SalI G'TCGA_C
Cuts at: 3546 3546
Size: 6509
SapI GCTCTTCn'nnn_
Cuts at: 2768 3473 4532 4742 2768
Size: 705 1059 210 4535
Fragments arranged by size:
4535 1059 705 210
SbfI CC_TGCA'GG
Cuts at: 3556 3556
Size: 6509
ScaI AGT'ACT
Cuts at: 2769 6026 2769
Size: 3257 3252
SexAI A'CCwGG_T
Cuts at: 3144 3144
Size: 6509
SfcI C'TryA_G
Cuts at: 33 447 889 1235 2346 2662 2719 2821 2859
Size: 414 442 346 1111 316 57 102 38
Cuts at: 2859 2968 3552 4237 5230 5421 33
Size: 109 584 685 993 191 1121
Fragments arranged by size:
1121 1111 993 685 584 442 414 346
316 191 109 102 57 38
SfoI GGC'GCC
Cuts at: 3941 4189 3941
Size: 248 6261
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6509
SmaI CCC'GGG
Cuts at: 3531 3531
Size: 6509
SmlI C'TyrA_G
Cuts at: 518 862 3664 4579 5071 5333 518
Size: 344 2802 915 492 262 1694
Fragments arranged by size:
2802 1694 915 492 344 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6509
SphI G_CATG'C
Cuts at: 3562 4062 3562
Size: 500 6009
SspI AAT'ATT
Cuts at: 1074 1411 1974 2142 2899 1074
Size: 337 563 168 757 4684
Fragments arranged by size:
4684 757 563 337 168
StuI AGG'CCT
Cuts at: 1692 2493 1692
Size: 801 5708
StyI C'CwwG_G
Cuts at: 107 246 1758 1764 2257 2595 3643 3997 107
Size: 139 1512 6 493 338 1048 354 2619
Fragments arranged by size:
2619 1512 1048 493 354 338 139 6
SwaI ATTT'AAAT
Cuts at: 2297 2297
Size: 6509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 414 4092 4225 4684 4893 414
Size: 3678 133 459 209 2030
Fragments arranged by size:
3678 2030 459 209 133
TatI w'GTAC_w
Cuts at: 441 1377 1490 2767 2843 2972 6024 441
Size: 936 113 1277 76 129 3052 926
Fragments arranged by size:
3052 1277 936 926 129 113 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2115 2147 6026 6058 2115
Size: 32 3879 32 2566
Fragments arranged by size:
3879 2566 32 32
Tth111I GACn'n_nGTC
Cuts at: 550 4306 550
Size: 3756 2753
XbaI T'CTAG_A
Cuts at: 2097 3540 2097
Size: 1443 5066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1147 4004 1147
Size: 2857 3652
XhoI C'TCGA_G
Cuts at: 518 518
Size: 6509
XmaI C'CCGG_G
Cuts at: 3529 3529
Size: 6509
XmnI GAAnn'nnTTC
Cuts at: 1516 1562 1875 1516
Size: 46 313 6150
Fragments arranged by size:
6150 313 46
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsrGI BssHII
BstEII BstZ17I ClaI EcoRV FseI FspAI MfeI NheI
NotI NruI PacI PasI PfoI PmlI PpuMI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI ZraI