Restriction map for
pF1KE3355
(Circular) (Six-base) MAPSORT of: pF1KE3355.seq Check: 653 from: 1 to: 6254
pF1KE3355 6254 bp
With 182 enzymes: SgfI *
August 27, 2018 19:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 139 139
Size: 6254
AatII G_ACGT'C
Cuts at: 2228 2228
Size: 6254
AccI GT'mk_AC
Cuts at: 2776 2813 3292 2776
Size: 37 479 5738
Fragments arranged by size:
5738 479 37
Acc65I G'GTAC_C
Cuts at: 3270 3270
Size: 6254
AclI AA'CG_TT
Cuts at: 6094 6094
Size: 6254
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1163 2618 2682 3043 3118 4079 4511 5258
Size: 358 1455 64 361 75 961 432 747
Cuts at: 5258 5526 805
Size: 268 1533
Fragments arranged by size:
1533 1455 961 747 432 361 358 268
75 64
AfeI AGC'GCT
Cuts at: 357 3095 357
Size: 2738 3516
AflIII A'CryG_T
Cuts at: 907 1761 3606 4710 5687 907
Size: 854 1845 1104 977 1474
Fragments arranged by size:
1845 1474 1104 977 854
AgeI A'CCGG_T
Cuts at: 1726 4642 1726
Size: 2916 3338
AleI CACnn'nnGTG
Cuts at: 1634 1634
Size: 6254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2761 2761
Size: 6254
AlwNI CAG_nnn'CTG
Cuts at: 1800 3559 5126 1800
Size: 1759 1567 2928
Fragments arranged by size:
2928 1759 1567
ApaI G_GGCC'C
Cuts at: 736 1087 736
Size: 351 5903
ApaLI G'TGCA_C
Cuts at: 5024 5024
Size: 6254
ApoI r'AATT_y
Cuts at: 1030 3258 5607 5863 1030
Size: 2228 2349 256 1421
Fragments arranged by size:
2349 2228 1421 256
AseI AT'TA_AT
Cuts at: 20 1979 20
Size: 1959 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6254
AvaI C'yCGr_G
Cuts at: 166 719 736 944 1058 1178 1341 3274 166
Size: 553 17 208 114 120 163 1933 3146
Fragments arranged by size:
3146 1933 553 208 163 120 114 17
BamHI G'GATC_C
Cuts at: 1127 1669 3279 1127
Size: 542 1610 4102
Fragments arranged by size:
4102 1610 542
BanI G'GyrC_C
Cuts at: 325 345 452 1174 1858 2488 3270 3684 3932
Size: 20 107 722 684 630 782 414 248
Cuts at: 3932 3967 325
Size: 35 2612
Fragments arranged by size:
2612 782 722 684 630 414 248 107
35 20
BanII G_rGCy'C
Cuts at: 736 1059 1087 1401 2418 3268 4298 736
Size: 323 28 314 1017 850 1030 2692
Fragments arranged by size:
2692 1030 1017 850 323 314 28
BbeI G_GCGC'C
Cuts at: 329 456 1862 3688 3936 329
Size: 127 1406 1826 248 2647
Fragments arranged by size:
2647 1826 1406 248 127
BbsI GAAGACnn'nnnn_
Cuts at: 852 1070 852
Size: 218 6036
BbvCI CC'TCA_GC
Cuts at: 2299 2669 2968 2299
Size: 370 299 5585
Fragments arranged by size:
5585 370 299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 919 953 919
Size: 34 6220
BciVI GTATCCnnnnn_n'
Cuts at: 4152 4913 6254 4152
Size: 761 1341 4152
Fragments arranged by size:
4152 1341 761
BclI T'GATC_A
Cuts at: 992 3774 992
Size: 2782 3472
BglI GCCn_nnn'nGGC
Cuts at: 430 2864 430
Size: 2434 3820
BglII A'GATC_T
Cuts at: 1712 3769 5806 1712
Size: 2057 2037 2160
Fragments arranged by size:
2160 2057 2037
BlpI GC'TnA_GC
Cuts at: 352 2429 3366 352
Size: 2077 937 3240
Fragments arranged by size:
3240 2077 937
Bme1580I G_kGCm'C
Cuts at: 285 584 736 1087 1495 3879 3972 5028 285
Size: 299 152 351 408 2384 93 1056 1511
Fragments arranged by size:
2384 1511 1056 408 351 299 152 93
BmrI ACTGGGnnnn_n'
Cuts at: 1247 3002 3466 3547 3882 6026 1247
Size: 1755 464 81 335 2144 1475
Fragments arranged by size:
2144 1755 1475 464 335 81
BmtI G_CTAG'C
Cuts at: 1574 1574
Size: 6254
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4621 4621
Size: 6254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1576 2599 817
Size: 759 1023 4472
Fragments arranged by size:
4472 1023 759
Bpu10I CC'TnA_GC
Cuts at: 214 2299 2417 2669 2968 214
Size: 2085 118 252 299 3500
Fragments arranged by size:
3500 2085 299 252 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 2026 3430 4309 4801 5099 352
Size: 1674 1404 879 492 298 1507
Fragments arranged by size:
1674 1507 1404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1295 5958 1295
Size: 4663 1591
BsaAI yAC'GTr
Cuts at: 910 998 3607 910
Size: 88 2609 3557
Fragments arranged by size:
3557 2609 88
BsaBI GATnn'nnATC
Cuts at: 6 3768 5805 6
Size: 3762 2037 455
Fragments arranged by size:
3762 2037 455
BsaHI Gr'CG_yC
Cuts at: 326 453 665 1859 2225 3685 3933 4635 6131
Size: 127 212 1194 366 1460 248 702 1496
Cuts at: 6131 326
Size: 449
Fragments arranged by size:
1496 1460 1194 702 449 366 248 212
127
BsaWI w'CCGG_w
Cuts at: 1726 2366 3443 3964 4642 4916 5063 5474 1726
Size: 640 1077 521 678 274 147 411 2506
Fragments arranged by size:
2506 1077 678 640 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2645 2675 3041 3071 2645
Size: 30 366 30 5828
Fragments arranged by size:
5828 366 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1391 3102 1391
Size: 1711 4543
BseYI C'CCAG_C
Cuts at: 552 1080 1592 2424 3007 3679 4437 5014 5510
Size: 528 512 832 583 672 758 577 496
Cuts at: 5510 552
Size: 1296
Fragments arranged by size:
1296 832 758 672 583 577 528 512
496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 917 1658 721
Size: 196 741 5317
Fragments arranged by size:
5317 741 196
BsiEI CG_ry'CG
Cuts at: 104 3796 3842 5050 104
Size: 3692 46 1208 1308
Fragments arranged by size:
3692 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2081 2740 3268 4046 4236 5028 2081
Size: 659 528 778 190 792 3307
Fragments arranged by size:
3307 792 778 659 528 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2818 2818
Size: 6254
Bsp1286I G_dGCh'C
Cuts at: 285 584 736 1059 1087 1401 1495 2081 2418
Size: 299 152 323 28 314 94 586 337
Cuts at: 2418 2740 3268 3879 3972 4046 4236 4298 5028
Size: 322 528 611 93 74 190 62 730
Cuts at: 5028 285
Size: 1511
Fragments arranged by size:
1511 730 611 586 528 337 323 322
314 299 190 152 94 93 74 62
28
BspEI T'CCGG_A
Cuts at: 5474 5474
Size: 6254
BspHI T'CATG_A
Cuts at: 1688 5430 1688
Size: 3742 2512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 139 305 398 1785 2739 2788 3304 3518 3820
Size: 166 93 1387 954 49 516 214 302
Cuts at: 3820 4201 139
Size: 381 2192
Fragments arranged by size:
2192 1387 954 516 381 302 214 166
93 49
BsrBI CCG'CTC
Cuts at: 50 2433 3225 4546 4600 6084 6249 50
Size: 2383 792 1321 54 1484 165 55
Fragments arranged by size:
2383 1484 1321 792 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4166 4166
Size: 6254
BsrFI r'CCGG_y
Cuts at: 638 920 1726 4252 4433 4642 638
Size: 282 806 2526 181 209 2250
Fragments arranged by size:
2526 2250 806 282 209 181
BsrGI T'GTAC_A
Cuts at: 2661 2661
Size: 6254
BssSI C'ACGA_G
Cuts at: 1907 4525 4883 1907
Size: 2618 358 3278
Fragments arranged by size:
3278 2618 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3631 3631
Size: 6254
BstBI TT'CG_AA
Cuts at: 1194 4615 1194
Size: 3421 2833
BstXI CCAn_nnnn'nTGG
Cuts at: 2058 2499 2541 2058
Size: 441 42 5771
Fragments arranged by size:
5771 441 42
BstYI r'GATC_y
Cuts at: 1 812 1127 1669 1712 3101 3279 3746 3769
Size: 811 315 542 43 1389 178 467 23
Cuts at: 3769 4104 4350 5362 5448 5460 5806 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1389 1012 811 542 467 449 346 335
315 246 178 86 43 23 12
BstZ17I GTA'TAC
Cuts at: 2777 2777
Size: 6254
BtgI C'CryG_G
Cuts at: 107 235 290 1295 1698 107
Size: 128 55 1005 403 4663
Fragments arranged by size:
4663 1005 403 128 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1089 2012 2421 4204 4385 4548 1089
Size: 923 409 1783 181 163 2795
Fragments arranged by size:
2795 1783 923 409 181 163
BtsI GCAGTG_nn'
Cuts at: 283 2274 3062 3497 283
Size: 1991 788 435 3040
Fragments arranged by size:
3040 1991 788 435
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2466 2501 2466
Size: 35 6219
DraI TTT'AAA
Cuts at: 3253 3253
Size: 6254
DraIII CAC_nnn'GTG
Cuts at: 711 711
Size: 6254
DrdI GACnn_nn'nnGTC
Cuts at: 3960 4818 3960
Size: 858 5396
EaeI y'GGCC_r
Cuts at: 232 2102 2341 2353 2434 3839 4013 4404 4431
Size: 1870 239 12 81 1405 174 391 27
Cuts at: 4431 5776 232
Size: 1345 710
Fragments arranged by size:
1870 1405 1345 710 391 239 174 81
27 12
EagI C'GGCC_G
Cuts at: 3839 3839
Size: 6254
EarI CTCTTCn'nnn_
Cuts at: 2378 4277 4487 2378
Size: 1899 210 4145
Fragments arranged by size:
4145 1899 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 4462 4772 4918 6064 2935
Size: 1527 310 146 1146 3125
Fragments arranged by size:
3125 1527 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3266 3266
Size: 6254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 817 1163 1576 2599 2618 2682 3043 3118
Size: 12 346 413 1023 19 64 361 75
Cuts at: 3118 4079 4511 5258 5526 805
Size: 961 432 747 268 1533
Fragments arranged by size:
1533 1023 961 747 432 413 361 346
268 75 64 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 820 967 1990 820
Size: 147 1023 5084
Fragments arranged by size:
5084 1023 147
EcoO109I rG'GnC_Cy
Cuts at: 244 400 675 732 733 901 960 1084 2289
Size: 156 275 57 1 168 59 124 1205
Cuts at: 2289 2421 3232 3393 244
Size: 132 811 161 3105
Fragments arranged by size:
3105 1205 811 275 168 161 156 132
124 59 57 1
EcoRI G'AATT_C
Cuts at: 1030 3258 1030
Size: 2228 4026
EcoRV GAT'ATC
Cuts at: 1225 1225
Size: 6254
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4273 4273
Size: 6254
FseI GG_CCGG'CC
Cuts at: 924 924
Size: 6254
FspI TGC'GCA
Cuts at: 2518 4035 2518
Size: 1517 4737
HaeII r_GCGC'y
Cuts at: 115 329 359 456 1862 2472 3097 3688 3936
Size: 214 30 97 1406 610 625 591 248
Cuts at: 3936 4958 5937 115
Size: 1022 979 432
Fragments arranged by size:
1406 1022 979 625 610 591 432 248
214 97 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 822 854 1429 1461 4096 4126 4128 4158 822
Size: 32 575 32 2635 30 2 30 2918
Fragments arranged by size:
2918 2635 575 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2878 3293 2878
Size: 415 5839
KasI G'GCGC_C
Cuts at: 325 452 1858 3684 3932 325
Size: 127 1406 1826 248 2647
Fragments arranged by size:
2647 1826 1406 248 127
KpnI G_GTAC'C
Cuts at: 3274 3274
Size: 6254
MfeI C'AATT_G
Cuts at: 588 1478 588
Size: 890 5364
MluI A'CGCG_T
Cuts at: 5687 5687
Size: 6254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2075 4925 5109 2075
Size: 2850 184 3220
Fragments arranged by size:
3220 2850 184
MscI TGG'CCA
Cuts at: 234 2104 4015 234
Size: 1870 1911 2473
Fragments arranged by size:
2473 1911 1870
MslI CAynn'nnrTG
Cuts at: 1634 2209 2743 4243 4370 1634
Size: 575 534 1500 127 3518
Fragments arranged by size:
3518 1500 575 534 127
MspA1I CmG'CkG
Cuts at: 250 351 2340 2428 2967 3365 3679 4039 5052
Size: 101 1989 88 539 398 314 360 1013
Cuts at: 5052 5297 250
Size: 245 1207
Fragments arranged by size:
1989 1207 1013 539 398 360 314 245
101 88
NaeI GCC'GGC
Cuts at: 922 4435 922
Size: 3513 2741
NarI GG'CG_CC
Cuts at: 326 453 1859 3685 3933 326
Size: 127 1406 1826 248 2647
Fragments arranged by size:
2647 1826 1406 248 127
NcoI C'CATG_G
Cuts at: 107 1295 1698 107
Size: 1188 403 4663
Fragments arranged by size:
4663 1188 403
NdeI CA'TA_TG
Cuts at: 1817 1817
Size: 6254
NgoMIV G'CCGG_C
Cuts at: 920 4433 920
Size: 3513 2741
NheI G'CTAG_C
Cuts at: 1570 1570
Size: 6254
NspI r_CATG'y
Cuts at: 1578 1765 2214 3307 3807 4714 1578
Size: 187 449 1093 500 907 3118
Fragments arranged by size:
3118 1093 907 500 449 187
PasI CC'CwG_GG
Cuts at: 1385 1385
Size: 6254
PciI A'CATG_T
Cuts at: 1761 4710 1761
Size: 2949 3305
PflMI CCAn_nnn'nTGG
Cuts at: 2443 2631 2443
Size: 188 6066
PfoI T'CCnGG_A
Cuts at: 3237 3237
Size: 6254
PmeI GTTT'AAAC
Cuts at: 3253 3253
Size: 6254
PmlI CAC'GTG
Cuts at: 910 910
Size: 6254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5393 5425 5393
Size: 32 6222
PpuMI rG'GwC_Cy
Cuts at: 244 400 2421 3232 244
Size: 156 2021 811 3266
Fragments arranged by size:
3266 2021 811 156
PspOMI G'GGCC_C
Cuts at: 732 1083 732
Size: 351 5903
PstI C_TGCA'G
Cuts at: 683 1332 1607 2252 3301 3986 683
Size: 649 275 645 1049 685 2951
Fragments arranged by size:
2951 1049 685 649 645 275
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6254
PvuII CAG'CTG
Cuts at: 2428 2967 3679 4039 2428
Size: 539 712 360 4643
Fragments arranged by size:
4643 712 539 360
RsrII CG'GwC_CG
Cuts at: 4449 4449
Size: 6254
SacI G_AGCT'C
Cuts at: 3268 3268
Size: 6254
SalI G'TCGA_C
Cuts at: 3291 3291
Size: 6254
SanDI GG'GwC_CC
Cuts at: 244 244
Size: 6254
SapI GCTCTTCn'nnn_
Cuts at: 2378 4277 4487 2378
Size: 1899 210 4145
Fragments arranged by size:
4145 1899 210
SbfI CC_TGCA'GG
Cuts at: 683 2252 3301 683
Size: 1569 1049 3636
Fragments arranged by size:
3636 1569 1049
ScaI AGT'ACT
Cuts at: 5771 5771
Size: 6254
SexAI A'CCwGG_T
Cuts at: 707 707
Size: 6254
SfcI C'TryA_G
Cuts at: 33 679 914 1328 1603 2229 2248 3297 3982
Size: 646 235 414 275 626 19 1049 685
Cuts at: 3982 4975 5166 33
Size: 993 191 1121
Fragments arranged by size:
1121 1049 993 685 646 626 414 275
235 191 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 2864 2864
Size: 6254
SfoI GGC'GCC
Cuts at: 327 454 1860 3686 3934 327
Size: 127 1406 1826 248 2647
Fragments arranged by size:
2647 1826 1406 248 127
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6254
SmaI CCC'GGG
Cuts at: 168 946 1060 1180 3276 168
Size: 778 114 120 2096 3146
Fragments arranged by size:
3146 2096 778 120 114
SmlI C'TyrA_G
Cuts at: 367 2041 3409 4324 4816 5078 367
Size: 1674 1368 915 492 262 1543
Fragments arranged by size:
1674 1543 1368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6254
SphI G_CATG'C
Cuts at: 2214 3307 3807 2214
Size: 1093 500 4661
Fragments arranged by size:
4661 1093 500
StuI AGG'CCT
Cuts at: 973 2572 2674 973
Size: 1599 102 4553
Fragments arranged by size:
4553 1599 102
StyI C'CwwG_G
Cuts at: 107 1295 1698 3122 3388 3742 107
Size: 1188 403 1424 266 354 2619
Fragments arranged by size:
2619 1424 1188 403 354 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 544 763 1283 1377 3837 3970 4429 4638 544
Size: 219 520 94 2460 133 459 209 2160
Fragments arranged by size:
2460 2160 520 459 219 209 133 94
TatI w'GTAC_w
Cuts at: 2661 5769 2661
Size: 3108 3146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 236 268 585 617 3041 3073 5771 5803 236
Size: 32 317 32 2424 32 2698 32 687
Fragments arranged by size:
2698 2424 687 317 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1282 4051 1282
Size: 2769 3485
XbaI T'CTAG_A
Cuts at: 1929 3285 1929
Size: 1356 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1302 2058 3749 1302
Size: 756 1691 3807
Fragments arranged by size:
3807 1691 756
XmaI C'CCGG_G
Cuts at: 166 944 1058 1178 3274 166
Size: 778 114 120 2096 3146
Fragments arranged by size:
3146 2096 778 120 114
ZraI GAC'GTC
Cuts at: 2226 2226
Size: 6254
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BfrBI BmgBI
BsiWI BsmI BssHII BstEII Bsu36I ClaI FspAI HindIII
HpaI NotI NruI NsiI PacI PshAI PsiI PspXI
PsrI SacII SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI