Restriction map for
pF1KE3350
(Circular) (Six-base) MAPSORT of: pF1KE3350.seq Check: 7583 from: 1 to: 6221
pF1KE3350 6221 bp
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 507 1219 2877 507
Size: 712 1658 3851
Fragments arranged by size:
3851 1658 712
AccI GT'mk_AC
Cuts at: 3259 3259
Size: 6221
Acc65I G'GTAC_C
Cuts at: 3237 3237
Size: 6221
AclI AA'CG_TT
Cuts at: 6061 6061
Size: 6221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1640 1772 2372 4046 4478 5225 5493 505
Size: 1135 132 600 1674 432 747 268 1233
Fragments arranged by size:
1674 1233 1135 747 600 432 268 132
AfeI AGC'GCT
Cuts at: 728 740 728
Size: 12 6209
AflIII A'CryG_T
Cuts at: 459 2725 3573 4677 5654 459
Size: 2266 848 1104 977 1026
Fragments arranged by size:
2266 1104 1026 977 848
AgeI A'CCGG_T
Cuts at: 2455 4609 2455
Size: 2154 4067
AhdI GACnn_n'nnGTC
Cuts at: 900 900
Size: 6221
AleI CACnn'nnGTG
Cuts at: 145 145
Size: 6221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 931 931
Size: 6221
AlwNI CAG_nnn'CTG
Cuts at: 1506 2164 2188 2836 3526 5093 1506
Size: 658 24 648 690 1567 2634
Fragments arranged by size:
2634 1567 690 658 648 24
ApaLI G'TGCA_C
Cuts at: 2283 2579 4991 2283
Size: 296 2412 3513
Fragments arranged by size:
3513 2412 296
ApoI r'AATT_y
Cuts at: 1379 1840 1860 1893 3205 3225 5574 5830 1379
Size: 461 20 33 1312 20 2349 256 1770
Fragments arranged by size:
2349 1770 1312 461 256 33 20 20
AscI GG'CGCG_CC
Cuts at: 565 565
Size: 6221
AseI AT'TA_AT
Cuts at: 20 20
Size: 6221
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
AvaI C'yCGr_G
Cuts at: 201 1430 1448 1740 3241 201
Size: 1229 18 292 1501 3181
Fragments arranged by size:
3181 1501 1229 292 18
BamHI G'GATC_C
Cuts at: 3246 3246
Size: 6221
BanI G'GyrC_C
Cuts at: 121 368 644 1024 3237 3651 3899 3934 121
Size: 247 276 380 2213 414 248 35 2408
Fragments arranged by size:
2408 2213 414 380 276 248 247 35
BanII G_rGCy'C
Cuts at: 168 494 3235 4265 168
Size: 326 2741 1030 2124
Fragments arranged by size:
2741 2124 1030 326
BbeI G_GCGC'C
Cuts at: 125 372 3655 3903 125
Size: 247 3283 248 2443
Fragments arranged by size:
3283 2443 248 247
BbsI GAAGACnn'nnnn_
Cuts at: 1052 2699 1052
Size: 1647 4574
BbvCI CC'TCA_GC
Cuts at: 2936 2936
Size: 6221
BciVI GTATCCnnnnn_n'
Cuts at: 4119 4880 6221 4119
Size: 761 1341 4119
Fragments arranged by size:
4119 1341 761
BclI T'GATC_A
Cuts at: 1052 3741 1052
Size: 2689 3532
BglI GCCn_nnn'nGGC
Cuts at: 441 559 1039 441
Size: 118 480 5623
Fragments arranged by size:
5623 480 118
BglII A'GATC_T
Cuts at: 2328 2970 3736 5773 2328
Size: 642 766 2037 2776
Fragments arranged by size:
2776 2037 766 642
BlpI GC'TnA_GC
Cuts at: 177 277 3333 177
Size: 100 3056 3065
Fragments arranged by size:
3065 3056 100
Bme1580I G_kGCm'C
Cuts at: 881 2287 2502 2583 3846 3939 4995 881
Size: 1406 215 81 1263 93 1056 2107
Fragments arranged by size:
2107 1406 1263 1056 215 93 81
BmgBI CAC'GTC
Cuts at: 462 1527 3117 462
Size: 1065 1590 3566
Fragments arranged by size:
3566 1590 1065
BmrI ACTGGGnnnn_n'
Cuts at: 834 3433 3514 3849 5993 834
Size: 2599 81 335 2144 1062
Fragments arranged by size:
2599 2144 1062 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2202 4588 2202
Size: 2386 3835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 727 1110 1552 2858 3217 499
Size: 228 383 442 1306 359 3503
Fragments arranged by size:
3503 1306 442 383 359 228
Bpu10I CC'TnA_GC
Cuts at: 1217 2936 1217
Size: 1719 4502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 883 943 1322 3397 4276 4768 5066 883
Size: 60 379 2075 879 492 298 2038
Fragments arranged by size:
2075 2038 879 492 379 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 2904 5925 2904
Size: 3021 3200
BsaAI yAC'GTr
Cuts at: 396 813 3574 396
Size: 417 2761 3043
Fragments arranged by size:
3043 2761 417
BsaBI GATnn'nnATC
Cuts at: 6 1637 2798 3735 5772 6
Size: 1631 1161 937 2037 455
Fragments arranged by size:
2037 1631 1161 937 455
BsaHI Gr'CG_yC
Cuts at: 122 320 369 3652 3900 4602 6098 122
Size: 198 49 3283 248 702 1496 245
Fragments arranged by size:
3283 1496 702 248 245 198 49
BsaWI w'CCGG_w
Cuts at: 1123 2455 2613 3410 3931 4609 4883 5030 5441
Size: 1332 158 797 521 678 274 147 411
Cuts at: 5441 1123
Size: 1903
Fragments arranged by size:
1903 1332 797 678 521 411 274 158
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 295 325 295
Size: 30 6191
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 189 880 1453 1492 2191 2864 3177 189
Size: 691 573 39 699 673 313 3233
Fragments arranged by size:
3233 699 691 673 573 313 39
BseYI C'CCAG_C
Cuts at: 445 502 2171 3646 4404 4981 5477 445
Size: 57 1669 1475 758 577 496 1189
Fragments arranged by size:
1669 1475 1189 758 577 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 853 871 1702 2831 3174 382
Size: 471 18 831 1129 343 3429
Fragments arranged by size:
3429 1129 831 471 343 18
BsiEI CG_ry'CG
Cuts at: 104 137 158 416 3763 3809 5017 104
Size: 33 21 258 3347 46 1208 1308
Fragments arranged by size:
3347 1308 1208 258 46 33 21
BsiHKAI G_wGCw'C
Cuts at: 494 1018 2287 2583 3235 4013 4203 4995 494
Size: 524 1269 296 652 778 190 792 1720
Fragments arranged by size:
1720 1269 792 778 652 524 296 190
BsmI GAATG_Cn'
Cuts at: 1249 2199 1249
Size: 950 5271
Bsp1286I G_dGCh'C
Cuts at: 168 494 881 1018 2287 2502 2583 3235 3846
Size: 326 387 137 1269 215 81 652 611
Cuts at: 3846 3939 4013 4203 4265 4995 168
Size: 93 74 190 62 730 1394
Fragments arranged by size:
1394 1269 730 652 611 387 326 215
190 137 93 81 74 62
BspEI T'CCGG_A
Cuts at: 2613 5441 2613
Size: 2828 3393
BspHI T'CATG_A
Cuts at: 1235 5397 1235
Size: 4162 2059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 507 866 1219 2877 3271 3485 3787 4168 507
Size: 359 353 1658 394 214 302 381 2560
Fragments arranged by size:
2560 1658 394 381 359 353 302 214
BsrBI CCG'CTC
Cuts at: 50 474 1536 2966 4513 4567 6051 6216 50
Size: 424 1062 1430 1547 54 1484 165 55
Fragments arranged by size:
1547 1484 1430 1062 424 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4133 4133
Size: 6221
BsrFI r'CCGG_y
Cuts at: 2455 3176 4219 4400 4609 2455
Size: 721 1043 181 209 4067
Fragments arranged by size:
4067 1043 721 209 181
BssHII G'CGCG_C
Cuts at: 565 771 773 791 793 565
Size: 206 2 18 2 5993
Fragments arranged by size:
5993 206 18 2 2
BssSI C'ACGA_G
Cuts at: 4492 4850 4492
Size: 358 5863
BstAPI GCAn_nnn'nTGC
Cuts at: 2188 2347 3019 3598 2188
Size: 159 672 579 4811
Fragments arranged by size:
4811 672 579 159
BstBI TT'CG_AA
Cuts at: 4582 4582
Size: 6221
BstXI CCAn_nnnn'nTGG
Cuts at: 396 2004 2295 396
Size: 1608 291 4322
Fragments arranged by size:
4322 1608 291
BstYI r'GATC_y
Cuts at: 1 2242 2328 2562 2970 3246 3713 3736 4071
Size: 2241 86 234 408 276 467 23 335
Cuts at: 4071 4317 5329 5415 5427 5773 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2241 1012 467 449 408 346 335 276
246 234 86 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2978 2978
Size: 6221
BtgI C'CryG_G
Cuts at: 107 125 717 1160 107
Size: 18 592 443 5168
Fragments arranged by size:
5168 592 443 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4171 4352 4515 4171
Size: 181 163 5877
Fragments arranged by size:
5877 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 2627 2848 3005 3464 1325
Size: 1302 221 157 459 4082
Fragments arranged by size:
4082 1302 459 221 157
DraI TTT'AAA
Cuts at: 1622 3220 1622
Size: 1598 4623
DraIII CAC_nnn'GTG
Cuts at: 2537 2931 2537
Size: 394 5827
DrdI GACnn_nn'nnGTC
Cuts at: 3000 3927 4785 3000
Size: 927 858 4436
Fragments arranged by size:
4436 927 858
EaeI y'GGCC_r
Cuts at: 155 291 413 551 743 3178 3806 3980 4371
Size: 136 122 138 192 2435 628 174 391
Cuts at: 4371 4398 5743 155
Size: 27 1345 633
Fragments arranged by size:
2435 1345 633 628 391 192 174 138
136 122 27
EagI C'GGCC_G
Cuts at: 155 413 3806 155
Size: 258 3393 2570
Fragments arranged by size:
3393 2570 258
EarI CTCTTCn'nnn_
Cuts at: 634 1430 1585 2036 2366 4244 4454 634
Size: 796 155 451 330 1878 210 2401
Fragments arranged by size:
2401 1878 796 451 330 210 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 737 1725 4429 4739 4885 6031 737
Size: 988 2704 310 146 1146 927
Fragments arranged by size:
2704 1146 988 927 310 146
EcoICRI GAG'CTC
Cuts at: 492 3233 492
Size: 2741 3480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 505 727 1110 1552 1640 1772 2372 2858
Size: 6 222 383 442 88 132 600 486
Cuts at: 2858 3217 4046 4478 5225 5493 499
Size: 359 829 432 747 268 1227
Fragments arranged by size:
1227 829 747 600 486 442 432 383
359 268 222 132 88 6
EcoNI CCTnn'n_nnAGG
Cuts at: 862 862
Size: 6221
EcoO109I rG'GnC_Cy
Cuts at: 573 894 2033 3360 573
Size: 321 1139 1327 3434
Fragments arranged by size:
3434 1327 1139 321
EcoRI G'AATT_C
Cuts at: 3205 3225 3205
Size: 20 6201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1080 4240 1080
Size: 3160 3061
FspI TGC'GCA
Cuts at: 4002 4002
Size: 6221
HaeII r_GCGC'y
Cuts at: 125 372 503 730 742 3655 3903 4925 5904
Size: 247 131 227 12 2913 248 1022 979
Cuts at: 5904 125
Size: 442
Fragments arranged by size:
2913 1022 979 442 248 247 227 131
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 886 918 1411 1443 1628 1660 1926
Size: 32 559 32 493 32 185 32 266
Cuts at: 1926 1958 2136 2168 2306 2338 2378 2410 2595
Size: 32 178 32 138 32 40 32 185
Cuts at: 2595 2627 4063 4093 4095 4125 295
Size: 32 1436 30 2 30 2391
Fragments arranged by size:
2391 1436 559 493 266 185 185 178
138 40 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 822 1301 1784 1970 3260 822
Size: 479 483 186 1290 3783
Fragments arranged by size:
3783 1290 483 479 186
KasI G'GCGC_C
Cuts at: 121 368 3651 3899 121
Size: 247 3283 248 2443
Fragments arranged by size:
3283 2443 248 247
KpnI G_GTAC'C
Cuts at: 3241 3241
Size: 6221
MluI A'CGCG_T
Cuts at: 5654 5654
Size: 6221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2827 4892 5076 2827
Size: 2065 184 3972
Fragments arranged by size:
3972 2065 184
MscI TGG'CCA
Cuts at: 3982 3982
Size: 6221
MslI CAynn'nnrTG
Cuts at: 145 1009 1756 1903 2215 4210 4337 145
Size: 864 747 147 312 1995 127 2029
Fragments arranged by size:
2029 1995 864 747 312 147 127
MspA1I CmG'CkG
Cuts at: 127 297 366 719 748 1506 2403 3009 3332
Size: 170 69 353 29 758 897 606 323
Cuts at: 3332 3646 4006 5019 5264 127
Size: 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 897 758 606 360 353 323
314 245 170 69 29
NaeI GCC'GGC
Cuts at: 3178 4402 3178
Size: 1224 4997
NarI GG'CG_CC
Cuts at: 122 369 3652 3900 122
Size: 247 3283 248 2443
Fragments arranged by size:
3283 2443 248 247
NcoI C'CATG_G
Cuts at: 107 107
Size: 6221
NgoMIV G'CCGG_C
Cuts at: 3176 4400 3176
Size: 1224 4997
NspI r_CATG'y
Cuts at: 852 2729 3274 3774 4681 852
Size: 1877 545 500 907 2392
Fragments arranged by size:
2392 1877 907 545 500
PasI CC'CwG_GG
Cuts at: 2502 2502
Size: 6221
PciI A'CATG_T
Cuts at: 2725 4677 2725
Size: 1952 4269
PflMI CCAn_nnn'nTGG
Cuts at: 300 549 2641 300
Size: 249 2092 3880
Fragments arranged by size:
3880 2092 249
PmeI GTTT'AAAC
Cuts at: 3220 3220
Size: 6221
PmlI CAC'GTG
Cuts at: 813 813
Size: 6221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5360 5392 5360
Size: 32 6189
PpuMI rG'GwC_Cy
Cuts at: 573 894 2033 573
Size: 321 1139 4761
Fragments arranged by size:
4761 1139 321
PstI C_TGCA'G
Cuts at: 683 1463 2160 2549 3268 3953 683
Size: 780 697 389 719 685 2951
Fragments arranged by size:
2951 780 719 697 685 389
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6221
PvuII CAG'CTG
Cuts at: 297 1506 3009 3646 4006 297
Size: 1209 1503 637 360 2512
Fragments arranged by size:
2512 1503 1209 637 360
RsrII CG'GwC_CG
Cuts at: 4416 4416
Size: 6221
SacI G_AGCT'C
Cuts at: 494 3235 494
Size: 2741 3480
SacII CC_GC'GG
Cuts at: 128 720 128
Size: 592 5629
SalI G'TCGA_C
Cuts at: 3258 3258
Size: 6221
SapI GCTCTTCn'nnn_
Cuts at: 634 2036 4244 4454 634
Size: 1402 2208 210 2401
Fragments arranged by size:
2401 2208 1402 210
SbfI CC_TGCA'GG
Cuts at: 3268 3268
Size: 6221
ScaI AGT'ACT
Cuts at: 5738 5738
Size: 6221
SfcI C'TryA_G
Cuts at: 33 679 1459 2156 2545 2716 3264 3949 4942
Size: 646 780 697 389 171 548 685 993
Cuts at: 4942 5133 33
Size: 191 1121
Fragments arranged by size:
1121 993 780 697 685 646 548 389
191 171
SfoI GGC'GCC
Cuts at: 123 370 3653 3901 123
Size: 247 3283 248 2443
Fragments arranged by size:
3283 2443 248 247
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
SgrAI Cr'CCGG_yG
Cuts at: 2455 2455
Size: 6221
SmaI CCC'GGG
Cuts at: 1432 3243 1432
Size: 1811 4410
SmlI C'TyrA_G
Cuts at: 898 922 1337 3376 4291 4783 5045 898
Size: 24 415 2039 915 492 262 2074
Fragments arranged by size:
2074 2039 915 492 415 262 24
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6221
SphI G_CATG'C
Cuts at: 852 3274 3774 852
Size: 2422 500 3299
Fragments arranged by size:
3299 2422 500
StuI AGG'CCT
Cuts at: 871 1687 2110 871
Size: 816 423 4982
Fragments arranged by size:
4982 816 423
StyI C'CwwG_G
Cuts at: 107 1580 1963 2003 2366 2890 3071 3355 3709
Size: 1473 383 40 363 524 181 284 354
Cuts at: 3709 107
Size: 2619
Fragments arranged by size:
2619 1473 524 383 363 354 284 181
40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 151 2270 2919 3070 3804 3937 4396 4605 151
Size: 2119 649 151 734 133 459 209 1767
Fragments arranged by size:
2119 1767 734 649 459 209 151 133
TatI w'GTAC_w
Cuts at: 5736 5736
Size: 6221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5738 5770 5738
Size: 32 6189
Tth111I GACn'n_nGTC
Cuts at: 2787 4018 2787
Size: 1231 4990
XbaI T'CTAG_A
Cuts at: 3252 3252
Size: 6221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3716 3716
Size: 6221
XmaI C'CCGG_G
Cuts at: 1430 3241 1430
Size: 1811 4410
XmnI GAAnn'nnTTC
Cuts at: 1346 3205 1346
Size: 1859 4362
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AloI ApaI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsmBI BsrGI BstEII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SnaBI SrfI SspI
SwaI XhoI ZraI