Restriction map for
pF1KE3328
(Circular) (Six-base) MAPSORT of: pF1KE3328.seq Check: 1420 from: 1 to: 5921
pF1KE3328 5921 bp
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AccI GT'mk_AC
Cuts at: 2959 2959
Size: 5921
Acc65I G'GTAC_C
Cuts at: 2937 2937
Size: 5921
AclI AA'CG_TT
Cuts at: 1881 5761 1881
Size: 3880 2041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 558 1305 1633 1768 2340 2549 2570 3746 4178
Size: 747 328 135 572 209 21 1176 432
Cuts at: 4178 4925 5193 558
Size: 747 268 1286
Fragments arranged by size:
1286 1176 747 747 572 432 328 268
209 135 21
AflIII A'CryG_T
Cuts at: 1561 3273 4377 5354 1561
Size: 1712 1104 977 2128
Fragments arranged by size:
2128 1712 1104 977
AgeI A'CCGG_T
Cuts at: 4309 4309
Size: 5921
AlwNI CAG_nnn'CTG
Cuts at: 241 2256 2425 3226 4793 241
Size: 2015 169 801 1567 1369
Fragments arranged by size:
2015 1567 1369 801 169
ApaLI G'TGCA_C
Cuts at: 2325 4691 2325
Size: 2366 3555
ApoI r'AATT_y
Cuts at: 211 420 973 1255 1378 2381 2396 2542 2925
Size: 209 553 282 123 1003 15 146 383
Cuts at: 2925 5274 5530 211
Size: 2349 256 602
Fragments arranged by size:
2349 1003 602 553 383 282 256 209
146 123 15
AseI AT'TA_AT
Cuts at: 20 439 2101 20
Size: 419 1662 3840
Fragments arranged by size:
3840 1662 419
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5921
AvaI C'yCGr_G
Cuts at: 2941 2941
Size: 5921
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2497 2530 2497
Size: 33 5888
BamHI G'GATC_C
Cuts at: 336 2946 336
Size: 2610 3311
BanI G'GyrC_C
Cuts at: 2937 3351 3599 3634 2937
Size: 414 248 35 5224
Fragments arranged by size:
5224 414 248 35
BanII G_rGCy'C
Cuts at: 686 2866 2935 3965 686
Size: 2180 69 1030 2642
Fragments arranged by size:
2642 2180 1030 69
BbeI G_GCGC'C
Cuts at: 3355 3603 3355
Size: 248 5673
BbsI GAAGACnn'nnnn_
Cuts at: 1118 2270 2346 2541 2806 1118
Size: 1152 76 195 265 4233
Fragments arranged by size:
4233 1152 265 195 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2311 2345 2311
Size: 34 5887
BciVI GTATCCnnnnn_n'
Cuts at: 796 2859 3819 4580 5921 796
Size: 2063 960 761 1341 796
Fragments arranged by size:
2063 1341 960 796 761
BclI T'GATC_A
Cuts at: 758 1389 3441 758
Size: 631 2052 3238
Fragments arranged by size:
3238 2052 631
BfrBI ATG'CAT
Cuts at: 2871 2871
Size: 5921
BglII A'GATC_T
Cuts at: 3436 5473 3436
Size: 2037 3884
BlpI GC'TnA_GC
Cuts at: 3033 3033
Size: 5921
Bme1580I G_kGCm'C
Cuts at: 384 1433 2329 3546 3639 4695 384
Size: 1049 896 1217 93 1056 1610
Fragments arranged by size:
1610 1217 1056 1049 896 93
BmrI ACTGGGnnnn_n'
Cuts at: 3133 3214 3549 5693 3133
Size: 81 335 2144 3361
Fragments arranged by size:
3361 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4288 4288
Size: 5921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 1688 365
Size: 1323 4598
Bpu10I CC'TnA_GC
Cuts at: 1821 1821
Size: 5921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 2398 2765 3097 3976 4468 4766 1085
Size: 1313 367 332 879 492 298 2240
Fragments arranged by size:
2240 1313 879 492 367 332 298
BsaI GGTCTCn'nnnn_
Cuts at: 1620 2630 5625 1620
Size: 1010 2995 1916
Fragments arranged by size:
2995 1916 1010
BsaAI yAC'GTr
Cuts at: 205 3274 205
Size: 3069 2852
BsaBI GATnn'nnATC
Cuts at: 6 1508 3435 5472 6
Size: 1502 1927 2037 455
Fragments arranged by size:
2037 1927 1502 455
BsaHI Gr'CG_yC
Cuts at: 3352 3600 4302 5798 3352
Size: 248 702 1496 3475
Fragments arranged by size:
3475 1496 702 248
BsaWI w'CCGG_w
Cuts at: 129 3110 3631 4309 4583 4730 5141 129
Size: 2981 521 678 274 147 411 909
Fragments arranged by size:
2981 909 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 872 902 872
Size: 30 5891
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1685 1738 2812 1685
Size: 53 1074 4794
Fragments arranged by size:
4794 1074 53
BseYI C'CCAG_C
Cuts at: 155 1528 2005 3346 4104 4681 5177 155
Size: 1373 477 1341 758 577 496 899
Fragments arranged by size:
1373 1341 899 758 577 496 477
BsiEI CG_ry'CG
Cuts at: 104 3463 3509 4717 104
Size: 3359 46 1208 1308
Fragments arranged by size:
3359 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2329 2866 2935 3713 3903 4695 2329
Size: 537 69 778 190 792 3555
Fragments arranged by size:
3555 792 778 537 190 69
BsmI GAATG_Cn'
Cuts at: 1101 1101
Size: 5921
Bsp1286I G_dGCh'C
Cuts at: 384 686 1433 2329 2866 2935 3546 3639 3713
Size: 302 747 896 537 69 611 93 74
Cuts at: 3713 3903 3965 4695 384
Size: 190 62 730 1610
Fragments arranged by size:
1610 896 747 730 611 537 302 190
93 74 69 62
BspEI T'CCGG_A
Cuts at: 129 5141 129
Size: 5012 909
BspHI T'CATG_A
Cuts at: 2064 2558 5097 2064
Size: 494 2539 2888
Fragments arranged by size:
2888 2539 494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 521 786 1523 2971 3185 3487 3868 521
Size: 265 737 1448 214 302 381 2574
Fragments arranged by size:
2574 1448 737 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 4213 4267 5751 5916 50
Size: 4163 54 1484 165 55
Fragments arranged by size:
4163 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 919 3833 919
Size: 2914 3007
BsrFI r'CCGG_y
Cuts at: 3919 4100 4309 3919
Size: 181 209 5531
Fragments arranged by size:
5531 209 181
BssSI C'ACGA_G
Cuts at: 4192 4550 4192
Size: 358 5563
BstAPI GCAn_nnn'nTGC
Cuts at: 3298 3298
Size: 5921
BstBI TT'CG_AA
Cuts at: 663 2711 4282 663
Size: 2048 1571 2302
Fragments arranged by size:
2302 2048 1571
BstXI CCAn_nnnn'nTGG
Cuts at: 2130 2764 2130
Size: 634 5287
BstYI r'GATC_y
Cuts at: 1 336 2946 3413 3436 3771 4017 5029 5115
Size: 335 2610 467 23 335 246 1012 86
Cuts at: 5115 5127 5473 1
Size: 12 346 449
Fragments arranged by size:
2610 1012 467 449 346 335 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2309 2309
Size: 5921
BtgI C'CryG_G
Cuts at: 107 1458 107
Size: 1351 4570
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3871 4052 4215 3871
Size: 181 163 5577
Fragments arranged by size:
5577 181 163
BtsI GCAGTG_nn'
Cuts at: 815 2329 2625 3164 815
Size: 1514 296 539 3572
Fragments arranged by size:
3572 1514 539 296
DraI TTT'AAA
Cuts at: 590 2920 590
Size: 2330 3591
DraIII CAC_nnn'GTG
Cuts at: 379 2324 379
Size: 1945 3976
DrdI GACnn_nn'nnGTC
Cuts at: 3627 4485 3627
Size: 858 5063
EaeI y'GGCC_r
Cuts at: 843 2676 3506 3680 4071 4098 5443 843
Size: 1833 830 174 391 27 1345 1321
Fragments arranged by size:
1833 1345 1321 830 391 174 27
EagI C'GGCC_G
Cuts at: 3506 3506
Size: 5921
EarI CTCTTCn'nnn_
Cuts at: 2068 2564 3944 4154 2068
Size: 496 1380 210 3835
Fragments arranged by size:
3835 1380 496 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 676 4129 4439 4585 5731 676
Size: 3453 310 146 1146 866
Fragments arranged by size:
3453 1146 866 310 146
EcoICRI GAG'CTC
Cuts at: 2864 2933 2864
Size: 69 5852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 558 1305 1633 1688 1768 2340 2549 2570
Size: 193 747 328 55 80 572 209 21
Cuts at: 2570 3746 4178 4925 5193 365
Size: 1176 432 747 268 1093
Fragments arranged by size:
1176 1093 747 747 572 432 328 268
209 193 80 55 21
EcoO109I rG'GnC_Cy
Cuts at: 123 2671 2738 3060 123
Size: 2548 67 322 2984
Fragments arranged by size:
2984 2548 322 67
EcoRI G'AATT_C
Cuts at: 211 420 2925 211
Size: 209 2505 3207
Fragments arranged by size:
3207 2505 209
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3940 3940
Size: 5921
FspI TGC'GCA
Cuts at: 3702 3702
Size: 5921
HaeII r_GCGC'y
Cuts at: 3355 3603 4625 5604 3355
Size: 248 1022 979 3672
Fragments arranged by size:
3672 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 750 782 872 904 2077 2109 2581 2613 3763
Size: 32 90 32 1173 32 472 32 1150
Cuts at: 3763 3793 3795 3825 750
Size: 30 2 30 2846
Fragments arranged by size:
2846 1173 1150 472 90 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2960 2960
Size: 5921
KasI G'GCGC_C
Cuts at: 3351 3599 3351
Size: 248 5673
KpnI G_GTAC'C
Cuts at: 2941 2941
Size: 5921
MluI A'CGCG_T
Cuts at: 5354 5354
Size: 5921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 780 987 2278 4592 4776 780
Size: 207 1291 2314 184 1925
Fragments arranged by size:
2314 1925 1291 207 184
MscI TGG'CCA
Cuts at: 845 3682 845
Size: 2837 3084
MslI CAynn'nnrTG
Cuts at: 449 3910 4037 449
Size: 3461 127 2333
Fragments arranged by size:
3461 2333 127
MspA1I CmG'CkG
Cuts at: 115 238 276 2422 3032 3346 3706 4719 4964
Size: 123 38 2146 610 314 360 1013 245
Cuts at: 4964 115
Size: 1072
Fragments arranged by size:
2146 1072 1013 610 360 314 245 123
38
NaeI GCC'GGC
Cuts at: 4102 4102
Size: 5921
NarI GG'CG_CC
Cuts at: 3352 3600 3352
Size: 248 5673
NcoI C'CATG_G
Cuts at: 107 107
Size: 5921
NdeI CA'TA_TG
Cuts at: 480 480
Size: 5921
NgoMIV G'CCGG_C
Cuts at: 4100 4100
Size: 5921
NsiI A_TGCA'T
Cuts at: 2873 2873
Size: 5921
NspI r_CATG'y
Cuts at: 1244 1565 2215 2525 2974 3474 4381 1244
Size: 321 650 310 449 500 907 2784
Fragments arranged by size:
2784 907 650 500 449 321 310
PciI A'CATG_T
Cuts at: 1561 4377 1561
Size: 2816 3105
PmeI GTTT'AAAC
Cuts at: 2920 2920
Size: 5921
PmlI CAC'GTG
Cuts at: 205 205
Size: 5921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5060 5092 5060
Size: 32 5889
PpuMI rG'GwC_Cy
Cuts at: 123 2671 2738 123
Size: 2548 67 3306
Fragments arranged by size:
3306 2548 67
PsiI TTA'TAA
Cuts at: 1622 1622
Size: 5921
PstI C_TGCA'G
Cuts at: 518 2968 3653 518
Size: 2450 685 2786
Fragments arranged by size:
2786 2450 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5921
PvuII CAG'CTG
Cuts at: 115 276 3346 3706 115
Size: 161 3070 360 2330
Fragments arranged by size:
3070 2330 360 161
RsrII CG'GwC_CG
Cuts at: 4116 4116
Size: 5921
SacI G_AGCT'C
Cuts at: 2866 2935 2866
Size: 69 5852
SalI G'TCGA_C
Cuts at: 2958 2958
Size: 5921
SanDI GG'GwC_CC
Cuts at: 123 123
Size: 5921
SapI GCTCTTCn'nnn_
Cuts at: 3944 4154 3944
Size: 210 5711
SbfI CC_TGCA'GG
Cuts at: 2968 2968
Size: 5921
ScaI AGT'ACT
Cuts at: 5438 5438
Size: 5921
SfcI C'TryA_G
Cuts at: 33 514 2248 2964 3649 4642 4833 33
Size: 481 1734 716 685 993 191 1121
Fragments arranged by size:
1734 1121 993 716 685 481 191
SfoI GGC'GCC
Cuts at: 3353 3601 3353
Size: 248 5673
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5921
SmaI CCC'GGG
Cuts at: 2943 2943
Size: 5921
SmlI C'TyrA_G
Cuts at: 1100 2413 2780 3076 3991 4483 4745 1100
Size: 1313 367 296 915 492 262 2276
Fragments arranged by size:
2276 1313 915 492 367 296 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5921
SphI G_CATG'C
Cuts at: 1244 2215 2974 3474 1244
Size: 971 759 500 3691
Fragments arranged by size:
3691 971 759 500
SspI AAT'ATT
Cuts at: 894 1053 1657 2190 2773 894
Size: 159 604 533 583 4042
Fragments arranged by size:
4042 604 583 533 159
StyI C'CwwG_G
Cuts at: 107 309 1268 1416 1703 3055 3409 107
Size: 202 959 148 287 1352 354 2619
Fragments arranged by size:
2619 1352 959 354 287 202 148
SwaI ATTT'AAAT
Cuts at: 590 590
Size: 5921
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 169 1975 2841 3504 3637 4096 4305 169
Size: 1806 866 663 133 459 209 1785
Fragments arranged by size:
1806 1785 866 663 459 209 133
TatI w'GTAC_w
Cuts at: 5436 5436
Size: 5921
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5438 5470 5438
Size: 32 5889
Tth111I GACn'n_nGTC
Cuts at: 1117 1372 3718 1117
Size: 255 2346 3320
Fragments arranged by size:
3320 2346 255
XbaI T'CTAG_A
Cuts at: 2952 2952
Size: 5921
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1423 1813 1914 3416 1423
Size: 390 101 1502 3928
Fragments arranged by size:
3928 1502 390 101
XmaI C'CCGG_G
Cuts at: 2941 2941
Size: 5921
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BglI BmgBI BmtI BsgI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PflMI PfoI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
StuI XhoI XmnI ZraI