Restriction map for
pF1KE3319
(Circular) (Six-base) MAPSORT of: pF1KE3319.seq Check: 347 from: 1 to: 5780
pF1KE3319 5780 bp
With 182 enzymes: SgfI *
March 2, 2011 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1140 2509 2700 1140
Size: 1369 191 4220
Fragments arranged by size:
4220 1369 191
AccI GT'mk_AC
Cuts at: 2818 2818
Size: 5780
Acc65I G'GTAC_C
Cuts at: 1317 2796 1317
Size: 1479 4301
AclI AA'CG_TT
Cuts at: 5620 5620
Size: 5780
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 481 589 893 1759 1897 2182 2221 2544
Size: 261 108 304 866 138 285 39 323
Cuts at: 2544 3605 4037 4784 5052 220
Size: 1061 432 747 268 948
Fragments arranged by size:
1061 948 866 747 432 323 304 285
268 261 138 108 39
AflIII A'CryG_T
Cuts at: 494 3132 4236 5213 494
Size: 2638 1104 977 1061
Fragments arranged by size:
2638 1104 1061 977
AgeI A'CCGG_T
Cuts at: 4168 4168
Size: 5780
AhdI GACnn_n'nnGTC
Cuts at: 2751 2751
Size: 5780
AleI CACnn'nnGTG
Cuts at: 1067 1067
Size: 5780
AlwNI CAG_nnn'CTG
Cuts at: 273 276 552 634 792 825 981 1329 3085
Size: 3 276 82 158 33 156 348 1756
Cuts at: 3085 4652 273
Size: 1567 1401
Fragments arranged by size:
1756 1567 1401 348 276 158 156 82
33 3
ApaLI G'TGCA_C
Cuts at: 4550 4550
Size: 5780
ApoI r'AATT_y
Cuts at: 2784 5133 5389 2784
Size: 2349 256 3175
Fragments arranged by size:
3175 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5780
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5780
AvaI C'yCGr_G
Cuts at: 869 1509 2077 2290 2512 2800 869
Size: 640 568 213 222 288 3849
Fragments arranged by size:
3849 640 568 288 222 213
AvrII C'CTAG_G
Cuts at: 1717 1717
Size: 5780
BamHI G'GATC_C
Cuts at: 1934 2805 1934
Size: 871 4909
BanI G'GyrC_C
Cuts at: 120 422 508 694 888 1317 1472 1961 2269
Size: 302 86 186 194 429 155 489 308
Cuts at: 2269 2796 3210 3458 3493 120
Size: 527 414 248 35 2407
Fragments arranged by size:
2407 527 489 429 414 308 302 248
194 186 155 86 35
BanII G_rGCy'C
Cuts at: 420 1203 2084 2794 3824 420
Size: 783 881 710 1030 2376
Fragments arranged by size:
2376 1030 881 783 710
BbeI G_GCGC'C
Cuts at: 124 698 1476 3214 3462 124
Size: 574 778 1738 248 2442
Fragments arranged by size:
2442 1738 778 574 248
BbsI GAAGACnn'nnnn_
Cuts at: 1612 2064 1612
Size: 452 5328
BbvCI CC'TCA_GC
Cuts at: 689 755 1309 689
Size: 66 554 5160
Fragments arranged by size:
5160 554 66
BciVI GTATCCnnnnn_n'
Cuts at: 889 3678 4439 5780 889
Size: 2789 761 1341 889
Fragments arranged by size:
2789 1341 889 761
BclI T'GATC_A
Cuts at: 3300 3300
Size: 5780
BglI GCCn_nnn'nGGC
Cuts at: 257 965 1018 257
Size: 708 53 5019
Fragments arranged by size:
5019 708 53
BglII A'GATC_T
Cuts at: 3295 5332 3295
Size: 2037 3743
BlpI GC'TnA_GC
Cuts at: 371 1791 1894 2892 371
Size: 1420 103 998 3259
Fragments arranged by size:
3259 1420 998 103
Bme1580I G_kGCm'C
Cuts at: 1964 2380 2500 3405 3498 4554 1964
Size: 416 120 905 93 1056 3190
Fragments arranged by size:
3190 1056 905 416 120 93
BmgBI CAC'GTC
Cuts at: 1729 1729
Size: 5780
BmrI ACTGGGnnnn_n'
Cuts at: 1580 2992 3073 3408 5552 1580
Size: 1412 81 335 2144 1808
Fragments arranged by size:
2144 1808 1412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4147 4147
Size: 5780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 625 847 1213 1231 1960 1986 2473 319
Size: 306 222 366 18 729 26 487 3626
Fragments arranged by size:
3626 729 487 366 306 222 26 18
Bpu10I CC'TnA_GC
Cuts at: 412 635 689 755 1309 412
Size: 223 54 66 554 4883
Fragments arranged by size:
4883 554 223 66 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 2488 2956 3835 4327 4625 1291
Size: 1197 468 879 492 298 2446
Fragments arranged by size:
2446 1197 879 492 468 298
BsaI GGTCTCn'nnnn_
Cuts at: 5484 5484
Size: 5780
BsaAI yAC'GTr
Cuts at: 497 2100 3133 497
Size: 1603 1033 3144
Fragments arranged by size:
3144 1603 1033
BsaBI GATnn'nnATC
Cuts at: 6 592 2056 3294 5331 6
Size: 586 1464 1238 2037 455
Fragments arranged by size:
2037 1464 1238 586 455
BsaHI Gr'CG_yC
Cuts at: 121 695 1473 2432 3211 3459 4161 5657 121
Size: 574 778 959 779 248 702 1496 244
Fragments arranged by size:
1496 959 779 778 702 574 248 244
BsaWI w'CCGG_w
Cuts at: 437 2249 2969 3490 4168 4442 4589 5000 437
Size: 1812 720 521 678 274 147 411 1217
Fragments arranged by size:
1812 1217 720 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 782 812 782
Size: 30 5750
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 664 1696 2476 2551 2698 127
Size: 537 1032 780 75 147 3209
Fragments arranged by size:
3209 1032 780 537 147 75
BseYI C'CCAG_C
Cuts at: 366 542 767 902 1005 1142 1800 2340 3205
Size: 176 225 135 103 137 658 540 865
Cuts at: 3205 3963 4540 5036 366
Size: 758 577 496 1110
Fragments arranged by size:
1110 865 758 658 577 540 496 225
176 137 135 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 313 652 1938 2678 286
Size: 27 339 1286 740 3388
Fragments arranged by size:
3388 1286 740 339 27
BsiEI CG_ry'CG
Cuts at: 104 397 1013 3322 3368 4576 104
Size: 293 616 2309 46 1208 1308
Fragments arranged by size:
2309 1308 1208 616 293 46
BsiHKAI G_wGCw'C
Cuts at: 1516 1895 2794 3572 3762 4554 1516
Size: 379 899 778 190 792 2742
Fragments arranged by size:
2742 899 792 778 379 190
Bsp1286I G_dGCh'C
Cuts at: 420 1203 1516 1895 1964 2084 2380 2500 2794
Size: 783 313 379 69 120 296 120 294
Cuts at: 2794 3405 3498 3572 3762 3824 4554 420
Size: 611 93 74 190 62 730 1646
Fragments arranged by size:
1646 783 730 611 379 313 296 294
190 120 120 93 74 69 62
BspEI T'CCGG_A
Cuts at: 437 5000 437
Size: 4563 1217
BspHI T'CATG_A
Cuts at: 1481 2048 4956 1481
Size: 567 2908 2305
Fragments arranged by size:
2908 2305 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 942 1140 1496 2509 2648 2700 2830 3044
Size: 709 198 356 1013 139 52 130 214
Cuts at: 3044 3346 3727 233
Size: 302 381 2286
Fragments arranged by size:
2286 1013 709 381 356 302 214 198
139 130 52
BsrBI CCG'CTC
Cuts at: 50 4072 4126 5610 5775 50
Size: 4022 54 1484 165 55
Fragments arranged by size:
4022 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3692 3692
Size: 5780
BsrFI r'CCGG_y
Cuts at: 3778 3959 4168 3778
Size: 181 209 5390
Fragments arranged by size:
5390 209 181
BssSI C'ACGA_G
Cuts at: 2062 2134 4051 4409 2062
Size: 72 1917 358 3433
Fragments arranged by size:
3433 1917 358 72
BstAPI GCAn_nnn'nTGC
Cuts at: 273 276 552 666 1329 1890 2583 3157 273
Size: 3 276 114 663 561 693 574 2896
Fragments arranged by size:
2896 693 663 574 561 276 114 3
BstBI TT'CG_AA
Cuts at: 4141 4141
Size: 5780
BstEII G'GTnAC_C
Cuts at: 1344 2041 1344
Size: 697 5083
BstXI CCAn_nnnn'nTGG
Cuts at: 705 705
Size: 5780
BstYI r'GATC_y
Cuts at: 1 1934 2057 2805 3272 3295 3630 3876 4888
Size: 1933 123 748 467 23 335 246 1012
Cuts at: 4888 4974 4986 5332 1
Size: 86 12 346 449
Fragments arranged by size:
1933 1012 748 467 449 346 335 246
123 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 384 1432 384
Size: 1048 4732
BtgI C'CryG_G
Cuts at: 107 124 397 107
Size: 17 273 5490
Fragments arranged by size:
5490 273 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3730 3911 4074 3730
Size: 181 163 5436
Fragments arranged by size:
5436 181 163
BtsI GCAGTG_nn'
Cuts at: 946 1555 2711 3023 946
Size: 609 1156 312 3703
Fragments arranged by size:
3703 1156 609 312
DraI TTT'AAA
Cuts at: 2779 2779
Size: 5780
DrdI GACnn_nn'nnGTC
Cuts at: 2419 3486 4344 2419
Size: 1067 858 3855
Fragments arranged by size:
3855 1067 858
EaeI y'GGCC_r
Cuts at: 236 394 1010 1019 1071 1154 3365 3539 3930
Size: 158 616 9 52 83 2211 174 391
Cuts at: 3930 3957 5302 236
Size: 27 1345 714
Fragments arranged by size:
2211 1345 714 616 391 174 158 83
52 27 9
EagI C'GGCC_G
Cuts at: 394 1010 3365 394
Size: 616 2355 2809
Fragments arranged by size:
2809 2355 616
EarI CTCTTCn'nnn_
Cuts at: 1616 2396 3803 4013 1616
Size: 780 1407 210 3383
Fragments arranged by size:
3383 1407 780 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 661 946 3988 4298 4444 5590 166
Size: 495 285 3042 310 146 1146 356
Fragments arranged by size:
3042 1146 495 356 310 285 146
EcoICRI GAG'CTC
Cuts at: 2792 2792
Size: 5780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 319 481 589 625 847 893 1213 1231
Size: 99 162 108 36 222 46 320 18
Cuts at: 1231 1759 1897 1960 1986 2182 2221 2473 2544
Size: 528 138 63 26 196 39 252 71
Cuts at: 2544 3605 4037 4784 5052 220
Size: 1061 432 747 268 948
Fragments arranged by size:
1061 948 747 528 432 320 268 252
222 196 162 138 108 99 71 63
46 39 36 26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1663 1663
Size: 5780
EcoO109I rG'GnC_Cy
Cuts at: 388 617 631 1824 2157 2919 388
Size: 229 14 1193 333 762 3249
Fragments arranged by size:
3249 1193 762 333 229 14
EcoRI G'AATT_C
Cuts at: 2784 2784
Size: 5780
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1629 3799 1629
Size: 2170 3610
FspI TGC'GCA
Cuts at: 856 3561 856
Size: 2705 3075
HaeII r_GCGC'y
Cuts at: 124 698 1002 1476 2511 3214 3462 4484 5463
Size: 574 304 474 1035 703 248 1022 979
Cuts at: 5463 124
Size: 441
Fragments arranged by size:
1035 1022 979 703 574 474 441 304
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1660 1692 2737 2769 3622 3652 3654 3684 1660
Size: 32 1045 32 853 30 2 30 3756
Fragments arranged by size:
3756 1045 853 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2424 2819 2424
Size: 395 5385
KasI G'GCGC_C
Cuts at: 120 694 1472 3210 3458 120
Size: 574 778 1738 248 2442
Fragments arranged by size:
2442 1738 778 574 248
KpnI G_GTAC'C
Cuts at: 1321 2800 1321
Size: 1479 4301
MluI A'CGCG_T
Cuts at: 5213 5213
Size: 5780
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 2147 4451 4635 311
Size: 1836 2304 184 1456
Fragments arranged by size:
2304 1836 1456 184
MscI TGG'CCA
Cuts at: 238 1021 3541 238
Size: 783 2520 2477
Fragments arranged by size:
2520 2477 783
MslI CAynn'nnrTG
Cuts at: 1067 2066 3769 3896 1067
Size: 999 1703 127 2951
Fragments arranged by size:
2951 1703 999 127
MspA1I CmG'CkG
Cuts at: 126 273 306 370 447 538 567 682 822
Size: 147 33 64 77 91 29 115 140
Cuts at: 822 1009 1787 2891 3205 3565 4578 4823 126
Size: 187 778 1104 314 360 1013 245 1083
Fragments arranged by size:
1104 1083 1013 778 360 314 245 187
147 140 115 91 77 64 33 29
NaeI GCC'GGC
Cuts at: 3961 3961
Size: 5780
NarI GG'CG_CC
Cuts at: 121 695 1473 3211 3459 121
Size: 574 778 1738 248 2442
Fragments arranged by size:
2442 1738 778 574 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5780
NdeI CA'TA_TG
Cuts at: 1709 1709
Size: 5780
NgoMIV G'CCGG_C
Cuts at: 3959 3959
Size: 5780
NotI GC'GGCC_GC
Cuts at: 1010 1010
Size: 5780
NspI r_CATG'y
Cuts at: 454 2833 3333 4240 454
Size: 2379 500 907 1994
Fragments arranged by size:
2379 1994 907 500
PasI CC'CwG_GG
Cuts at: 355 2190 2386 355
Size: 1835 196 3749
Fragments arranged by size:
3749 1835 196
PciI A'CATG_T
Cuts at: 4236 4236
Size: 5780
PflMI CCAn_nnn'nTGG
Cuts at: 2573 2573
Size: 5780
PfoI T'CCnGG_A
Cuts at: 1280 1937 2735 2765 1280
Size: 657 798 30 4295
Fragments arranged by size:
4295 798 657 30
PmeI GTTT'AAAC
Cuts at: 2779 2779
Size: 5780
PmlI CAC'GTG
Cuts at: 497 497
Size: 5780
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2397 2644 2676 4919 4951 2365
Size: 32 247 32 2243 32 3194
Fragments arranged by size:
3194 2243 247 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 388 617 1824 2157 388
Size: 229 1207 333 4011
Fragments arranged by size:
4011 1207 333 229
PshAI GACnn'nnGTC
Cuts at: 1180 1180
Size: 5780
PstI C_TGCA'G
Cuts at: 953 1388 1504 2126 2827 3512 953
Size: 435 116 622 701 685 3221
Fragments arranged by size:
3221 701 685 622 435 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5780
PvuII CAG'CTG
Cuts at: 273 306 370 447 538 567 682 822 3205
Size: 33 64 77 91 29 115 140 2383
Cuts at: 3205 3565 273
Size: 360 2488
Fragments arranged by size:
2488 2383 360 140 115 91 77 64
33 29
RsrII CG'GwC_CG
Cuts at: 3975 3975
Size: 5780
SacI G_AGCT'C
Cuts at: 2794 2794
Size: 5780
SacII CC_GC'GG
Cuts at: 127 127
Size: 5780
SalI G'TCGA_C
Cuts at: 2817 2817
Size: 5780
SanDI GG'GwC_CC
Cuts at: 617 617
Size: 5780
SapI GCTCTTCn'nnn_
Cuts at: 3803 4013 3803
Size: 210 5570
SbfI CC_TGCA'GG
Cuts at: 2827 2827
Size: 5780
ScaI AGT'ACT
Cuts at: 5297 5297
Size: 5780
SfcI C'TryA_G
Cuts at: 33 655 772 949 1384 1500 2122 2823 3508
Size: 622 117 177 435 116 622 701 685
Cuts at: 3508 4501 4692 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 701 685 622 622 435 191
177 117 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 1018 1018
Size: 5780
SfoI GGC'GCC
Cuts at: 122 696 1474 3212 3460 122
Size: 574 778 1738 248 2442
Fragments arranged by size:
2442 1738 778 574 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5780
SmaI CCC'GGG
Cuts at: 2802 2802
Size: 5780
SmlI C'TyrA_G
Cuts at: 1270 2467 2935 3850 4342 4604 1270
Size: 1197 468 915 492 262 2446
Fragments arranged by size:
2446 1197 915 492 468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5780
SphI G_CATG'C
Cuts at: 2833 3333 2833
Size: 500 5280
StuI AGG'CCT
Cuts at: 1567 1567
Size: 5780
StyI C'CwwG_G
Cuts at: 107 515 812 1052 1613 1717 2914 3268 107
Size: 408 297 240 561 104 1197 354 2619
Fragments arranged by size:
2619 1197 561 408 354 297 240 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1606 1653 2332 2359 3363 3496 3955 4164 1606
Size: 47 679 27 1004 133 459 209 3222
Fragments arranged by size:
3222 1004 679 459 209 133 47 27
TatI w'GTAC_w
Cuts at: 1919 5295 1919
Size: 3376 2404
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 938 970 1288 1320 1686 1713 1718 1745 5297
Size: 32 318 32 366 27 5 27 3552
Cuts at: 5297 5329 938
Size: 32 1389
Fragments arranged by size:
3552 1389 366 318 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 3577 3577
Size: 5780
XbaI T'CTAG_A
Cuts at: 2811 2811
Size: 5780
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 294 1095 3275 294
Size: 801 2180 2799
Fragments arranged by size:
2799 2180 801
XmaI C'CCGG_G
Cuts at: 2800 2800
Size: 5780
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI BaeI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NruI NsiI PacI PsiI PspOMI
PspXI PsrI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI