Restriction map for
pF1KE3268
(Circular) (Six-base) MAPSORT of: pF1KE3268.seq Check: 9197 from: 1 to: 5111
pF1KE3268 5111 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1542 2149 1542
Size: 607 4504
Acc65I G'GTAC_C
Cuts at: 2127 2127
Size: 5111
AclI AA'CG_TT
Cuts at: 1731 4951 1731
Size: 3220 1891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1563 2936 3368 4115 4383 1058
Size: 505 1373 432 747 268 1786
Fragments arranged by size:
1786 1373 747 505 432 268
AflIII A'CryG_T
Cuts at: 2463 3567 4544 2463
Size: 1104 977 3030
Fragments arranged by size:
3030 1104 977
AgeI A'CCGG_T
Cuts at: 3499 3499
Size: 5111
AhdI GACnn_n'nnGTC
Cuts at: 1555 1555
Size: 5111
AleI CACnn'nnGTG
Cuts at: 1015 1015
Size: 5111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1471 1471
Size: 5111
AlwNI CAG_nnn'CTG
Cuts at: 193 925 2416 3983 193
Size: 732 1491 1567 1321
Fragments arranged by size:
1567 1491 1321 732
ApaLI G'TGCA_C
Cuts at: 3881 3881
Size: 5111
ApoI r'AATT_y
Cuts at: 588 639 789 840 1297 2115 4464 4720 588
Size: 51 150 51 457 818 2349 256 979
Fragments arranged by size:
2349 979 818 457 256 150 51 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 5111
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5111
AvaI C'yCGr_G
Cuts at: 2131 2131
Size: 5111
BamHI G'GATC_C
Cuts at: 2136 2136
Size: 5111
BanI G'GyrC_C
Cuts at: 1017 2127 2541 2789 2824 1017
Size: 1110 414 248 35 3304
Fragments arranged by size:
3304 1110 414 248 35
BanII G_rGCy'C
Cuts at: 1265 2125 3155 1265
Size: 860 1030 3221
Fragments arranged by size:
3221 1030 860
BbeI G_GCGC'C
Cuts at: 2545 2793 2545
Size: 248 4863
BbsI GAAGACnn'nnnn_
Cuts at: 464 1097 464
Size: 633 4478
BbvCI CC'TCA_GC
Cuts at: 689 689
Size: 5111
BciVI GTATCCnnnnn_n'
Cuts at: 1370 3009 3770 5111 1370
Size: 1639 761 1341 1370
Fragments arranged by size:
1639 1370 1341 761
BclI T'GATC_A
Cuts at: 195 243 2075 2631 195
Size: 48 1832 556 2675
Fragments arranged by size:
2675 1832 556 48
BglII A'GATC_T
Cuts at: 2626 4663 2626
Size: 2037 3074
BlpI GC'TnA_GC
Cuts at: 155 1801 2223 155
Size: 1646 422 3043
Fragments arranged by size:
3043 1646 422
Bme1580I G_kGCm'C
Cuts at: 612 2736 2829 3885 612
Size: 2124 93 1056 1838
Fragments arranged by size:
2124 1838 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2323 2404 2739 4883 2323
Size: 81 335 2144 2551
Fragments arranged by size:
2551 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3478 3478
Size: 5111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1091 1091
Size: 5111
Bpu10I CC'TnA_GC
Cuts at: 689 689
Size: 5111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2287 3166 3658 3956 2287
Size: 879 492 298 3442
Fragments arranged by size:
3442 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1165 4815 1165
Size: 3650 1461
BsaAI yAC'GTr
Cuts at: 1750 2464 1750
Size: 714 4397
BsaBI GATnn'nnATC
Cuts at: 6 194 419 1663 2625 4662 6
Size: 188 225 1244 962 2037 455
Fragments arranged by size:
2037 1244 962 455 225 188
BsaHI Gr'CG_yC
Cuts at: 140 2542 2790 3492 4988 140
Size: 2402 248 702 1496 263
Fragments arranged by size:
2402 1496 702 263 248
BsaWI w'CCGG_w
Cuts at: 2017 2300 2821 3499 3773 3920 4331 2017
Size: 283 521 678 274 147 411 2797
Fragments arranged by size:
2797 678 521 411 283 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 694 739 1171 1852 1855 1867 616
Size: 78 45 432 681 3 12 3860
Fragments arranged by size:
3860 681 432 78 45 12 3
BseYI C'CCAG_C
Cuts at: 2536 3294 3871 4367 2536
Size: 758 577 496 3280
Fragments arranged by size:
3280 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1079 1079
Size: 5111
BsiEI CG_ry'CG
Cuts at: 104 757 2653 2699 3907 104
Size: 653 1896 46 1208 1308
Fragments arranged by size:
1896 1308 1208 653 46
BsiHKAI G_wGCw'C
Cuts at: 2125 2903 3093 3885 2125
Size: 778 190 792 3351
Fragments arranged by size:
3351 792 778 190
BsmI GAATG_Cn'
Cuts at: 210 885 981 1699 1898 210
Size: 675 96 718 199 3423
Fragments arranged by size:
3423 718 675 199 96
Bsp1286I G_dGCh'C
Cuts at: 612 1265 2125 2736 2829 2903 3093 3155 3885
Size: 653 860 611 93 74 190 62 730
Cuts at: 3885 612
Size: 1838
Fragments arranged by size:
1838 860 730 653 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2017 4331 2017
Size: 2314 2797
BspHI T'CATG_A
Cuts at: 4287 4287
Size: 5111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2161 2375 2677 3058 2161
Size: 214 302 381 4214
Fragments arranged by size:
4214 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3403 3457 4941 5106 50
Size: 3353 54 1484 165 55
Fragments arranged by size:
3353 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1422 1473 3023 1422
Size: 51 1550 3510
Fragments arranged by size:
3510 1550 51
BsrFI r'CCGG_y
Cuts at: 756 3109 3290 3499 756
Size: 2353 181 209 2368
Fragments arranged by size:
2368 2353 209 181
BsrGI T'GTAC_A
Cuts at: 1484 1484
Size: 5111
BssSI C'ACGA_G
Cuts at: 3382 3740 3382
Size: 358 4753
BstAPI GCAn_nnn'nTGC
Cuts at: 2488 2488
Size: 5111
BstBI TT'CG_AA
Cuts at: 3472 3472
Size: 5111
BstXI CCAn_nnnn'nTGG
Cuts at: 1815 1866 1815
Size: 51 5060
BstYI r'GATC_y
Cuts at: 1 123 178 616 2136 2603 2626 2961 3207
Size: 122 55 438 1520 467 23 335 246
Cuts at: 3207 4219 4305 4317 4663 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1520 1012 467 449 438 346 335 246
122 86 55 23 12
Bsu36I CC'TnA_GG
Cuts at: 1139 1139
Size: 5111
BtgI C'CryG_G
Cuts at: 107 1040 107
Size: 933 4178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2096 3061 3242 3405 2096
Size: 965 181 163 3802
Fragments arranged by size:
3802 965 181 163
BtsI GCAGTG_nn'
Cuts at: 942 1495 2354 942
Size: 553 859 3699
Fragments arranged by size:
3699 859 553
ClaI AT'CG_AT
Cuts at: 1827 1827
Size: 5111
DraI TTT'AAA
Cuts at: 1563 2110 1563
Size: 547 4564
DrdI GACnn_nn'nnGTC
Cuts at: 2817 3675 2817
Size: 858 4253
EaeI y'GGCC_r
Cuts at: 754 2696 2870 3261 3288 4633 754
Size: 1942 174 391 27 1345 1232
Fragments arranged by size:
1942 1345 1232 391 174 27
EagI C'GGCC_G
Cuts at: 754 2696 754
Size: 1942 3169
EarI CTCTTCn'nnn_
Cuts at: 133 821 893 1077 1148 2081 3134 3344 133
Size: 688 72 184 71 933 1053 210 1900
Fragments arranged by size:
1900 1053 933 688 210 184 72 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3319 3629 3775 4921 127
Size: 3192 310 146 1146 317
Fragments arranged by size:
3192 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 2123 2123
Size: 5111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1091 1563 2936 3368 4115 4383 1058
Size: 33 472 1373 432 747 268 1786
Fragments arranged by size:
1786 1373 747 472 432 268 33
EcoNI CCTnn'n_nnAGG
Cuts at: 1106 1106
Size: 5111
EcoO109I rG'GnC_Cy
Cuts at: 2250 2250
Size: 5111
EcoRI G'AATT_C
Cuts at: 639 789 2115 639
Size: 150 1326 3635
Fragments arranged by size:
3635 1326 150
EcoRV GAT'ATC
Cuts at: 1068 1068
Size: 5111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3130 3130
Size: 5111
FspI TGC'GCA
Cuts at: 2892 2892
Size: 5111
HaeII r_GCGC'y
Cuts at: 1770 1808 2545 2793 3815 4794 1770
Size: 38 737 248 1022 979 2087
Fragments arranged by size:
2087 1022 979 737 248 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 331 352 363 384 901 933 1054
Size: 32 136 21 11 21 517 32 121
Cuts at: 1054 1086 1541 1573 1904 1930 1936 1962 2953
Size: 32 455 32 331 26 6 26 991
Cuts at: 2953 2983 2985 3015 163
Size: 30 2 30 2259
Fragments arranged by size:
2259 991 517 455 331 136 121 32
32 32 32 30 30 26 26 21
21 11 6 2
HincII GTy'rAC
Cuts at: 2150 2150
Size: 5111
HindIII A'AGCT_T
Cuts at: 374 374
Size: 5111
KasI G'GCGC_C
Cuts at: 2541 2789 2541
Size: 248 4863
KpnI G_GTAC'C
Cuts at: 2131 2131
Size: 5111
MluI A'CGCG_T
Cuts at: 4544 4544
Size: 5111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3782 3966 3782
Size: 184 4927
MscI TGG'CCA
Cuts at: 2872 2872
Size: 5111
MslI CAynn'nnrTG
Cuts at: 329 527 890 1015 1708 3100 3227 329
Size: 198 363 125 693 1392 127 2213
Fragments arranged by size:
2213 1392 693 363 198 127 125
MspA1I CmG'CkG
Cuts at: 816 1794 2222 2536 2896 3909 4154 816
Size: 978 428 314 360 1013 245 1773
Fragments arranged by size:
1773 1013 978 428 360 314 245
NaeI GCC'GGC
Cuts at: 3292 3292
Size: 5111
NarI GG'CG_CC
Cuts at: 2542 2790 2542
Size: 248 4863
NcoI C'CATG_G
Cuts at: 107 1040 107
Size: 933 4178
NgoMIV G'CCGG_C
Cuts at: 3290 3290
Size: 5111
NspI r_CATG'y
Cuts at: 1452 2164 2664 3571 1452
Size: 712 500 907 2992
Fragments arranged by size:
2992 907 712 500
PciI A'CATG_T
Cuts at: 3567 3567
Size: 5111
PflMI CCAn_nnn'nTGG
Cuts at: 1678 1678
Size: 5111
PfoI T'CCnGG_A
Cuts at: 1107 1107
Size: 5111
PmeI GTTT'AAAC
Cuts at: 2110 2110
Size: 5111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4282 4250
Size: 32 5079
PshAI GACnn'nnGTC
Cuts at: 464 464
Size: 5111
PsiI TTA'TAA
Cuts at: 1525 1525
Size: 5111
PstI C_TGCA'G
Cuts at: 638 1502 2158 2843 638
Size: 864 656 685 2906
Fragments arranged by size:
2906 864 685 656
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5111
PvuII CAG'CTG
Cuts at: 816 2536 2896 816
Size: 1720 360 3031
Fragments arranged by size:
3031 1720 360
RsrII CG'GwC_CG
Cuts at: 3306 3306
Size: 5111
SacI G_AGCT'C
Cuts at: 2125 2125
Size: 5111
SalI G'TCGA_C
Cuts at: 2148 2148
Size: 5111
SapI GCTCTTCn'nnn_
Cuts at: 3134 3344 3134
Size: 210 4901
SbfI CC_TGCA'GG
Cuts at: 2158 2158
Size: 5111
ScaI AGT'ACT
Cuts at: 4628 4628
Size: 5111
SexAI A'CCwGG_T
Cuts at: 1336 1336
Size: 5111
SfcI C'TryA_G
Cuts at: 33 634 1498 1543 2154 2839 3832 4023 33
Size: 601 864 45 611 685 993 191 1121
Fragments arranged by size:
1121 993 864 685 611 601 191 45
SfoI GGC'GCC
Cuts at: 2543 2791 2543
Size: 248 4863
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5111
SmaI CCC'GGG
Cuts at: 2133 2133
Size: 5111
SmlI C'TyrA_G
Cuts at: 2266 3181 3673 3935 2266
Size: 915 492 262 3442
Fragments arranged by size:
3442 915 492 262
SnaBI TAC'GTA
Cuts at: 1750 1750
Size: 5111
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5111
SphI G_CATG'C
Cuts at: 2164 2664 2164
Size: 500 4611
StuI AGG'CCT
Cuts at: 687 865 687
Size: 178 4933
StyI C'CwwG_G
Cuts at: 107 1040 2245 2599 107
Size: 933 1205 354 2619
Fragments arranged by size:
2619 1205 933 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2694 2827 3286 3495 2694
Size: 133 459 209 4310
Fragments arranged by size:
4310 459 209 133
TatI w'GTAC_w
Cuts at: 1484 4626 1484
Size: 3142 1969
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4628 4660 4628
Size: 32 5079
Tth111I GACn'n_nGTC
Cuts at: 1623 2908 1623
Size: 1285 3826
XbaI T'CTAG_A
Cuts at: 2142 2142
Size: 5111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2606 114
Size: 2492 2619
XmaI C'CCGG_G
Cuts at: 2131 2131
Size: 5111
XmnI GAAnn'nnTTC
Cuts at: 1923 1923
Size: 5111
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BssHII BstEII BstZ17I CspCI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI