Restriction map for
pF1KE3218
(Circular) (Six-base) MAPSORT of: pF1KE3218.seq Check: 8890 from: 1 to: 4598
pF1KE3218 4598 bp
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 1614 1614
Size: 4598
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 748 990 1253 2423 2855 3602 3870 212
Size: 536 242 263 1170 432 747 268 940
Fragments arranged by size:
1170 940 747 536 432 268 263 242
AflIII A'CryG_T
Cuts at: 1950 3054 4031 1950
Size: 1104 977 2517
Fragments arranged by size:
2517 1104 977
AgeI A'CCGG_T
Cuts at: 2986 2986
Size: 4598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 439 1017 439
Size: 578 4020
AlwNI CAG_nnn'CTG
Cuts at: 1903 3470 1903
Size: 1567 3031
ApaLI G'TGCA_C
Cuts at: 1372 3368 1372
Size: 1996 2602
ApoI r'AATT_y
Cuts at: 789 1602 3951 4207 789
Size: 813 2349 256 1180
Fragments arranged by size:
2349 1180 813 256
AscI GG'CGCG_CC
Cuts at: 1538 1538
Size: 4598
AseI AT'TA_AT
Cuts at: 20 20
Size: 4598
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 209 1532 1618 209
Size: 1323 86 3189
Fragments arranged by size:
3189 1323 86
BamHI G'GATC_C
Cuts at: 600 950 1238 1623 600
Size: 350 288 385 3575
Fragments arranged by size:
3575 385 350 288
BanI G'GyrC_C
Cuts at: 958 1614 2028 2276 2311 958
Size: 656 414 248 35 3245
Fragments arranged by size:
3245 656 414 248 35
BanII G_rGCy'C
Cuts at: 1531 1612 2642 1531
Size: 81 1030 3487
Fragments arranged by size:
3487 1030 81
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BbsI GAAGACnn'nnnn_
Cuts at: 141 200 449 736 141
Size: 59 249 287 4003
Fragments arranged by size:
4003 287 249 59
BciVI GTATCCnnnnn_n'
Cuts at: 2496 3257 4598 2496
Size: 761 1341 2496
Fragments arranged by size:
2496 1341 761
BclI T'GATC_A
Cuts at: 2118 2118
Size: 4598
BfrBI ATG'CAT
Cuts at: 481 481
Size: 4598
BglI GCCn_nnn'nGGC
Cuts at: 1486 1486
Size: 4598
BglII A'GATC_T
Cuts at: 2113 4150 2113
Size: 2037 2561
BlpI GC'TnA_GC
Cuts at: 1710 1710
Size: 4598
Bme1580I G_kGCm'C
Cuts at: 1376 2223 2316 3372 1376
Size: 847 93 1056 2602
Fragments arranged by size:
2602 1056 847 93
BmrI ACTGGGnnnn_n'
Cuts at: 1493 1810 1891 2226 4370 1493
Size: 317 81 335 2144 1721
Fragments arranged by size:
2144 1721 335 317 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 848 1449 418
Size: 430 601 3567
Fragments arranged by size:
3567 601 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1774 2653 3145 3443 727
Size: 1047 879 492 298 1882
Fragments arranged by size:
1882 1047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4302 4302
Size: 4598
BsaAI yAC'GTr
Cuts at: 1341 1951 1341
Size: 610 3988
BsaBI GATnn'nnATC
Cuts at: 6 2112 4149 6
Size: 2106 2037 455
Fragments arranged by size:
2106 2037 455
BsaHI Gr'CG_yC
Cuts at: 2029 2277 2979 4475 2029
Size: 248 702 1496 2152
Fragments arranged by size:
2152 1496 702 248
BsaWI w'CCGG_w
Cuts at: 779 947 1787 2308 2986 3260 3407 3818 779
Size: 168 840 521 678 274 147 411 1559
Fragments arranged by size:
1559 840 678 521 411 274 168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1465 1495 1567 1597 1465
Size: 30 72 30 4466
Fragments arranged by size:
4466 72 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 279 1106 279
Size: 827 3771
BseYI C'CCAG_C
Cuts at: 280 507 800 885 1094 1521 2023 2781 3358
Size: 227 293 85 209 427 502 758 577
Cuts at: 3358 3854 280
Size: 496 1024
Fragments arranged by size:
1024 758 577 502 496 427 293 227
209 85
BsiEI CG_ry'CG
Cuts at: 104 624 2140 2186 3394 104
Size: 520 1516 46 1208 1308
Fragments arranged by size:
1516 1308 1208 520 46
BsiHKAI G_wGCw'C
Cuts at: 1376 1612 2390 2580 3372 1376
Size: 236 778 190 792 2602
Fragments arranged by size:
2602 792 778 236 190
BsmBI CGTCTCn'nnnn_
Cuts at: 562 562
Size: 4598
Bsp1286I G_dGCh'C
Cuts at: 1376 1531 1612 2223 2316 2390 2580 2642 3372
Size: 155 81 611 93 74 190 62 730
Cuts at: 3372 1376
Size: 2602
Fragments arranged by size:
2602 730 611 190 155 93 81 74
62
BspEI T'CCGG_A
Cuts at: 947 3818 947
Size: 2871 1727
BspHI T'CATG_A
Cuts at: 1212 3774 1212
Size: 2562 2036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 725 1367 1648 1862 2164 2545 725
Size: 642 281 214 302 381 2778
Fragments arranged by size:
2778 642 381 302 281 214
BsrBI CCG'CTC
Cuts at: 50 226 1139 2890 2944 4428 4593 50
Size: 176 913 1751 54 1484 165 55
Fragments arranged by size:
1751 1484 913 176 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2510 2510
Size: 4598
BsrFI r'CCGG_y
Cuts at: 659 923 1058 2596 2777 2986 659
Size: 264 135 1538 181 209 2271
Fragments arranged by size:
2271 1538 264 209 181 135
BssHII G'CGCG_C
Cuts at: 1538 1538
Size: 4598
BssSI C'ACGA_G
Cuts at: 547 1375 2869 3227 547
Size: 828 1494 358 1918
Fragments arranged by size:
1918 1494 828 358
BstAPI GCAn_nnn'nTGC
Cuts at: 938 1975 938
Size: 1037 3561
BstBI TT'CG_AA
Cuts at: 591 2959 591
Size: 2368 2230
BstYI r'GATC_y
Cuts at: 1 600 950 1238 1623 2090 2113 2448 2694
Size: 599 350 288 385 467 23 335 246
Cuts at: 2694 3706 3792 3804 4150 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 599 467 449 385 350 346 335
288 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1303 1303
Size: 4598
BtgI C'CryG_G
Cuts at: 240 240
Size: 4598
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2548 2729 2892 2548
Size: 181 163 4254
Fragments arranged by size:
4254 181 163
BtsI GCAGTG_nn'
Cuts at: 1229 1406 1841 1229
Size: 177 435 3986
Fragments arranged by size:
3986 435 177
DraI TTT'AAA
Cuts at: 1597 1597
Size: 4598
DraIII CAC_nnn'GTG
Cuts at: 1316 1316
Size: 4598
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3162 2304
Size: 858 3740
EaeI y'GGCC_r
Cuts at: 237 2183 2357 2748 2775 4120 237
Size: 1946 174 391 27 1345 715
Fragments arranged by size:
1946 1345 715 391 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 670 2621 2831 670
Size: 1951 210 2437
Fragments arranged by size:
2437 1951 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 597 2806 3116 3262 4408 597
Size: 2209 310 146 1146 787
Fragments arranged by size:
2209 1146 787 310 146
EcoICRI GAG'CTC
Cuts at: 1610 1610
Size: 4598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 418 748 848 990 1253 1449 2423 2855
Size: 206 330 100 142 263 196 974 432
Cuts at: 2855 3602 3870 212
Size: 747 268 940
Fragments arranged by size:
974 940 747 432 330 268 263 206
196 142 100
EcoO109I rG'GnC_Cy
Cuts at: 284 1029 1299 1569 1737 284
Size: 745 270 270 168 3145
Fragments arranged by size:
3145 745 270 270 168
EcoRI G'AATT_C
Cuts at: 789 1602 789
Size: 813 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 4598
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 2032 2280 3302 4281 2032
Size: 248 1022 979 2349
Fragments arranged by size:
2349 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 244 270 276 617 649 2440 2470 2472
Size: 6 26 6 341 32 1791 30 2
Cuts at: 2472 2502 238
Size: 30 2334
Fragments arranged by size:
2334 1791 341 32 30 30 26 6
6 2
HincII GTy'rAC
Cuts at: 263 1037 1637 263
Size: 774 600 3224
Fragments arranged by size:
3224 774 600
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 1618 1618
Size: 4598
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 669 3269 3453 669
Size: 2600 184 1814
Fragments arranged by size:
2600 1814 184
MscI TGG'CCA
Cuts at: 2359 2359
Size: 4598
MslI CAynn'nnrTG
Cuts at: 245 1343 2587 2714 245
Size: 1098 1244 127 2129
Fragments arranged by size:
2129 1244 1098 127
MspA1I CmG'CkG
Cuts at: 280 507 563 1709 2023 2383 3396 3641 280
Size: 227 56 1146 314 360 1013 245 1237
Fragments arranged by size:
1237 1146 1013 360 314 245 227 56
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NsiI A_TGCA'T
Cuts at: 483 483
Size: 4598
NspI r_CATG'y
Cuts at: 936 1372 1651 2151 3058 936
Size: 436 279 500 907 2476
Fragments arranged by size:
2476 907 500 436 279
PciI A'CATG_T
Cuts at: 3054 3054
Size: 4598
PfoI T'CCnGG_A
Cuts at: 434 434
Size: 4598
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PmlI CAC'GTG
Cuts at: 1341 1341
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4566
PpuMI rG'GwC_Cy
Cuts at: 284 1029 1299 1569 284
Size: 745 270 270 3313
Fragments arranged by size:
3313 745 270 270
PstI C_TGCA'G
Cuts at: 943 1645 2330 943
Size: 702 685 3211
Fragments arranged by size:
3211 702 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 280 507 563 2023 2383 280
Size: 227 56 1460 360 2495
Fragments arranged by size:
2495 1460 360 227 56
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 1612 1612
Size: 4598
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 670 2621 2831 670
Size: 1951 210 2437
Fragments arranged by size:
2437 1951 210
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 4115 4115
Size: 4598
SfcI C'TryA_G
Cuts at: 33 939 1587 1641 2326 3319 3510 33
Size: 906 648 54 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 648 191 54
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 211 1534 1620 211
Size: 1323 86 3189
Fragments arranged by size:
3189 1323 86
SmlI C'TyrA_G
Cuts at: 742 1753 2668 3160 3422 742
Size: 1011 915 492 262 1918
Fragments arranged by size:
1918 1011 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 936 1651 2151 936
Size: 715 500 3383
Fragments arranged by size:
3383 715 500
StyI C'CwwG_G
Cuts at: 169 611 1484 1732 2086 169
Size: 442 873 248 354 2681
Fragments arranged by size:
2681 873 442 354 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 638 926 2181 2314 2773 2982 638
Size: 288 1255 133 459 209 2254
Fragments arranged by size:
2254 1255 459 288 209 133
TatI w'GTAC_w
Cuts at: 1009 4113 1009
Size: 3104 1494
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 808 840 942 974 1048 1065 1080 1097 1379
Size: 32 102 32 74 17 15 17 282
Cuts at: 1379 1411 4115 4147 808
Size: 32 2704 32 1259
Fragments arranged by size:
2704 1259 282 102 74 32 32 32
32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2093 2093
Size: 4598
XmaI C'CCGG_G
Cuts at: 209 1532 1618 209
Size: 1323 86 3189
Fragments arranged by size:
3189 1323 86
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsgI
BsiWI BsmI BsrGI BstEII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI