Restriction map for
pF1KE3209
(Circular) (Six-base) MAPSORT of: pF1KE3209.seq Check: 1622 from: 1 to: 4547
pF1KE3209 4547 bp
With 182 enzymes: SgfI *
June 13, 2018 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 629 1155 629
Size: 526 4021
AccI GT'mk_AC
Cuts at: 434 1585 434
Size: 1151 3396
Acc65I G'GTAC_C
Cuts at: 1244 1563 1244
Size: 319 4228
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 396 563 1270 1519 2372 2804 3551 3819 396
Size: 167 707 249 853 432 747 268 1124
Fragments arranged by size:
1124 853 747 707 432 268 249 167
AfeI AGC'GCT
Cuts at: 542 542
Size: 4547
AflIII A'CryG_T
Cuts at: 1514 1899 3003 3980 1514
Size: 385 1104 977 2081
Fragments arranged by size:
2081 1104 977 385
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AlwNI CAG_nnn'CTG
Cuts at: 156 621 1170 1852 3419 156
Size: 465 549 682 1567 1284
Fragments arranged by size:
1567 1284 682 549 465
ApaI G_GGCC'C
Cuts at: 149 219 313 604 998 149
Size: 70 94 291 394 3698
Fragments arranged by size:
3698 394 291 94 70
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 135 142 536 544 801 928 1567 135
Size: 7 394 8 257 127 639 3115
Fragments arranged by size:
3115 639 394 257 127 8 7
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 281 687 807 1244 1563 1977 2225 2260 281
Size: 406 120 437 319 414 248 35 2568
Fragments arranged by size:
2568 437 414 406 319 248 120 35
BanII G_rGCy'C
Cuts at: 149 219 313 604 875 935 998 1430 1561
Size: 70 94 291 271 60 63 432 131
Cuts at: 1561 2591 149
Size: 1030 2105
Fragments arranged by size:
2105 1030 432 291 271 131 94 70
63 60
BbeI G_GCGC'C
Cuts at: 691 1981 2229 691
Size: 1290 248 3009
Fragments arranged by size:
3009 1290 248
BbsI GAAGACnn'nnnn_
Cuts at: 1192 1192
Size: 4547
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3206 4547 2445
Size: 761 1341 2445
Fragments arranged by size:
2445 1341 761
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 374 531 755 985 374
Size: 157 224 230 3936
Fragments arranged by size:
3936 230 224 157
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 1659 1659
Size: 4547
Bme1580I G_kGCm'C
Cuts at: 149 219 313 604 812 998 2172 2265 3321
Size: 70 94 291 208 186 1174 93 1056
Cuts at: 3321 149
Size: 1375
Fragments arranged by size:
1375 1174 1056 291 208 186 94 93
70
BmrI ACTGGGnnnn_n'
Cuts at: 1001 1759 1840 2175 4319 1001
Size: 758 81 335 2144 1229
Fragments arranged by size:
2144 1229 758 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 4547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 985 259
Size: 726 3821
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1723 2602 3094 3392 1723
Size: 879 492 298 2878
Fragments arranged by size:
2878 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 917 4251 917
Size: 3334 1213
BsaAI yAC'GTr
Cuts at: 1900 1900
Size: 4547
BsaBI GATnn'nnATC
Cuts at: 6 1481 2061 4098 6
Size: 1475 580 2037 455
Fragments arranged by size:
2037 1475 580 455
BsaHI Gr'CG_yC
Cuts at: 688 1978 2226 2928 4424 688
Size: 1290 248 702 1496 811
Fragments arranged by size:
1496 1290 811 702 248
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3209 3356 3767 1736
Size: 521 678 274 147 411 2516
Fragments arranged by size:
2516 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 805 757
Size: 48 4499
BseYI C'CCAG_C
Cuts at: 149 313 776 817 1081 1972 2730 3307 3803
Size: 164 463 41 264 891 758 577 496
Cuts at: 3803 149
Size: 893
Fragments arranged by size:
893 891 758 577 496 463 264 164
41
BsiEI CG_ry'CG
Cuts at: 104 245 299 385 2089 2135 3343 104
Size: 141 54 86 1704 46 1208 1308
Fragments arranged by size:
1704 1308 1208 141 86 54 46
BsiHKAI G_wGCw'C
Cuts at: 1237 1561 2339 2529 3321 1237
Size: 324 778 190 792 2463
Fragments arranged by size:
2463 792 778 324 190
Bsp1286I G_dGCh'C
Cuts at: 149 219 313 604 812 875 935 998 1237
Size: 70 94 291 208 63 60 63 239
Cuts at: 1237 1430 1561 2172 2265 2339 2529 2591 3321
Size: 193 131 611 93 74 190 62 730
Cuts at: 3321 149
Size: 1375
Fragments arranged by size:
1375 730 611 291 239 208 193 190
131 94 93 74 70 63 63 62
60
BspEI T'CCGG_A
Cuts at: 3767 3767
Size: 4547
BspHI T'CATG_A
Cuts at: 3723 3723
Size: 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 629 1155 1597 1811 2113 2494 629
Size: 526 442 214 302 381 2682
Fragments arranged by size:
2682 526 442 381 302 214
BsrBI CCG'CTC
Cuts at: 50 124 2839 2893 4377 4542 50
Size: 74 2715 54 1484 165 55
Fragments arranged by size:
2715 1484 165 74 55 54
BsrDI GCAATG_nn'
Cuts at: 2459 2459
Size: 4547
BsrFI r'CCGG_y
Cuts at: 202 368 2545 2726 2935 202
Size: 166 2177 181 209 1814
Fragments arranged by size:
2177 1814 209 181 166
BssHII G'CGCG_C
Cuts at: 377 1041 377
Size: 664 3883
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 1924 1924
Size: 4547
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 684 858 882 684
Size: 174 24 4349
Fragments arranged by size:
4349 174 24
BstYI r'GATC_y
Cuts at: 1 270 1572 2039 2062 2397 2643 3655 3741
Size: 269 1302 467 23 335 246 1012 86
Cuts at: 3741 3753 4099 1
Size: 12 346 449
Fragments arranged by size:
1302 1012 467 449 346 335 269 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 239 562 669 677 1520 107
Size: 132 323 107 8 843 3134
Fragments arranged by size:
3134 843 323 132 107 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2497 2678 2841 2497
Size: 181 163 4203
Fragments arranged by size:
4203 181 163
BtsI GCAGTG_nn'
Cuts at: 232 893 1219 1790 232
Size: 661 326 571 2989
Fragments arranged by size:
2989 661 571 326
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 200 242 296 382 497 650 860 1108 1154
Size: 42 54 86 115 153 210 248 46
Cuts at: 1154 2132 2306 2697 2724 4069 200
Size: 978 174 391 27 1345 678
Fragments arranged by size:
1345 978 678 391 248 210 174 153
115 86 54 46 42 27
EagI C'GGCC_G
Cuts at: 242 296 382 2132 242
Size: 54 86 1750 2657
Fragments arranged by size:
2657 1750 86 54
EarI CTCTTCn'nnn_
Cuts at: 631 1474 2570 2780 631
Size: 843 1096 210 2398
Fragments arranged by size:
2398 1096 843 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 762 2755 3065 3211 4357 762
Size: 1993 310 146 1146 952
Fragments arranged by size:
1993 1146 952 310 146
EcoICRI GAG'CTC
Cuts at: 1559 1559
Size: 4547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 396 563 985 1270 1519 2372 2804 3551
Size: 137 167 422 285 249 853 432 747
Cuts at: 3551 3819 259
Size: 268 987
Fragments arranged by size:
987 853 747 432 422 285 268 249
167 137
EcoNI CCTnn'n_nnAGG
Cuts at: 709 709
Size: 4547
EcoO109I rG'GnC_Cy
Cuts at: 139 146 215 216 310 798 995 1464 1686
Size: 7 69 1 94 488 197 469 222
Cuts at: 1686 139
Size: 3000
Fragments arranged by size:
3000 488 469 222 197 94 69 7
1
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1275 2566 1275
Size: 1291 3256
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 320 544 691 1099 1981 2229 3251 4230 320
Size: 224 147 408 882 248 1022 979 637
Fragments arranged by size:
1022 979 882 637 408 248 224 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1344 1376 2389 2419 2421 2451 1344
Size: 32 1013 30 2 30 3440
Fragments arranged by size:
3440 1013 32 30 30 2
HincII GTy'rAC
Cuts at: 435 1586 435
Size: 1151 3396
KasI G'GCGC_C
Cuts at: 687 1977 2225 687
Size: 1290 248 3009
Fragments arranged by size:
3009 1290 248
KpnI G_GTAC'C
Cuts at: 1248 1567 1248
Size: 319 4228
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1391 3218 3402 1391
Size: 1827 184 2536
Fragments arranged by size:
2536 1827 184
MscI TGG'CCA
Cuts at: 862 2308 862
Size: 1446 3101
MslI CAynn'nnrTG
Cuts at: 1519 2536 2663 1519
Size: 1017 127 3403
Fragments arranged by size:
3403 1017 127
MspA1I CmG'CkG
Cuts at: 153 241 279 564 591 671 780 958 1074
Size: 88 38 285 27 80 109 178 116
Cuts at: 1074 1658 1972 2332 3345 3590 153
Size: 584 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 584 360 314 285 245 178
116 109 88 80 38 27
NaeI GCC'GGC
Cuts at: 204 370 2728 204
Size: 166 2358 2023
Fragments arranged by size:
2358 2023 166
NarI GG'CG_CC
Cuts at: 688 1978 2226 688
Size: 1290 248 3009
Fragments arranged by size:
3009 1290 248
NcoI C'CATG_G
Cuts at: 107 677 107
Size: 570 3977
NgoMIV G'CCGG_C
Cuts at: 202 368 2726 202
Size: 166 2358 2023
Fragments arranged by size:
2358 2023 166
NotI GC'GGCC_GC
Cuts at: 242 296 242
Size: 54 4493
NspI r_CATG'y
Cuts at: 345 1334 1518 1600 2100 3007 345
Size: 989 184 82 500 907 1885
Fragments arranged by size:
1885 989 907 500 184 82
PasI CC'CwG_GG
Cuts at: 286 597 286
Size: 311 4236
PciI A'CATG_T
Cuts at: 1514 3003 1514
Size: 1489 3058
PflMI CCAn_nnn'nTGG
Cuts at: 327 327
Size: 4547
PfoI T'CCnGG_A
Cuts at: 191 1001 1237 1472 191
Size: 810 236 235 3266
Fragments arranged by size:
3266 810 236 235
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4515
PpuMI rG'GwC_Cy
Cuts at: 139 798 1464 139
Size: 659 666 3222
Fragments arranged by size:
3222 666 659
PspOMI G'GGCC_C
Cuts at: 145 215 309 600 994 145
Size: 70 94 291 394 3698
Fragments arranged by size:
3698 394 291 94 70
PspXI vC'TCGA_Gb
Cuts at: 536 544 536
Size: 8 4539
PstI C_TGCA'G
Cuts at: 260 899 1594 2279 260
Size: 639 695 685 2528
Fragments arranged by size:
2528 695 685 639
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 153 780 1074 1972 2332 153
Size: 627 294 898 360 2368
Fragments arranged by size:
2368 898 627 360 294
RsrII CG'GwC_CG
Cuts at: 402 2742 402
Size: 2340 2207
SacI G_AGCT'C
Cuts at: 1561 1561
Size: 4547
SacII CC_GC'GG
Cuts at: 242 565 672 242
Size: 323 107 4117
Fragments arranged by size:
4117 323 107
SalI G'TCGA_C
Cuts at: 433 1584 433
Size: 1151 3396
SanDI GG'GwC_CC
Cuts at: 139 798 139
Size: 659 3888
SapI GCTCTTCn'nnn_
Cuts at: 631 2570 2780 631
Size: 1939 210 2398
Fragments arranged by size:
2398 1939 210
SbfI CC_TGCA'GG
Cuts at: 260 1594 260
Size: 1334 3213
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SfcI C'TryA_G
Cuts at: 33 256 895 1590 2275 3268 3459 33
Size: 223 639 695 685 993 191 1121
Fragments arranged by size:
1121 993 695 685 639 223 191
SfoI GGC'GCC
Cuts at: 689 1979 2227 689
Size: 1290 248 3009
Fragments arranged by size:
3009 1290 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 137 144 803 1569 137
Size: 7 659 766 3115
Fragments arranged by size:
3115 766 659 7
SmlI C'TyrA_G
Cuts at: 536 544 1702 2617 3109 3371 536
Size: 8 1158 915 492 262 1712
Fragments arranged by size:
1712 1158 915 492 262 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StyI C'CwwG_G
Cuts at: 107 677 716 1458 1681 2035 107
Size: 570 39 742 223 354 2619
Fragments arranged by size:
2619 742 570 354 223 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2130 2263 2722 2931 2130
Size: 133 459 209 3746
Fragments arranged by size:
3746 459 209 133
TatI w'GTAC_w
Cuts at: 362 4062 362
Size: 3700 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1043 1075 4064 4096 1043
Size: 32 2989 32 1494
Fragments arranged by size:
2989 1494 32 32
Tth111I GACn'n_nGTC
Cuts at: 328 2344 328
Size: 2016 2531
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 283 426 1518 2042 283
Size: 143 1092 524 2788
Fragments arranged by size:
2788 1092 524 143
XhoI C'TCGA_G
Cuts at: 536 544 536
Size: 8 4539
XmaI C'CCGG_G
Cuts at: 135 142 801 1567 135
Size: 7 659 766 3115
Fragments arranged by size:
3115 766 659 7
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PmlI PshAI
PsiI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI