Restriction map for
pF1KE3182
(Circular) (Six-base) MAPSORT of: pF1KE3182.seq Check: 6093 from: 1 to: 4370
pF1KE3182 4370 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 151 1408 151
Size: 1257 3113
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2627 3374 3642 2195
Size: 432 747 268 2923
Fragments arranged by size:
2923 747 432 268
AfeI AGC'GCT
Cuts at: 475 475
Size: 4370
AflIII A'CryG_T
Cuts at: 146 1289 1722 2826 3803 146
Size: 1143 433 1104 977 713
Fragments arranged by size:
1143 1104 977 713 433
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AhdI GACnn_n'nnGTC
Cuts at: 765 765
Size: 4370
AleI CACnn'nnGTG
Cuts at: 715 715
Size: 4370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 832 832
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 588 852 1242 1675 3242 588
Size: 264 390 433 1567 1716
Fragments arranged by size:
1716 1567 433 390 264
ApaLI G'TGCA_C
Cuts at: 1093 3140 1093
Size: 2047 2323
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 182 396 541 1390 182
Size: 214 145 849 3162
Fragments arranged by size:
3162 849 214 145
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 178 226 1386 1800 2048 2083 178
Size: 48 1160 414 248 35 2465
Fragments arranged by size:
2465 1160 414 248 48 35
BanII G_rGCy'C
Cuts at: 200 353 548 1384 2414 200
Size: 153 195 836 1030 2156
Fragments arranged by size:
2156 1030 836 195 153
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 184 218 184
Size: 34 4336
BciVI GTATCCnnnnn_n'
Cuts at: 2268 3029 4370 2268
Size: 761 1341 2268
Fragments arranged by size:
2268 1341 761
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BglI GCCn_nnn'nGGC
Cuts at: 731 1106 731
Size: 375 3995
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 1267 1482 1267
Size: 215 4155
Bme1580I G_kGCm'C
Cuts at: 181 1097 1995 2088 3144 181
Size: 916 898 93 1056 1407
Fragments arranged by size:
1407 1056 916 898 93
BmgBI CAC'GTC
Cuts at: 900 900
Size: 4370
BmrI ACTGGGnnnn_n'
Cuts at: 1023 1582 1663 1998 4142 1023
Size: 559 81 335 2144 1251
Fragments arranged by size:
2144 1251 559 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 1032 1375 319
Size: 713 343 3314
Fragments arranged by size:
3314 713 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2425 2917 3215 1546
Size: 879 492 298 2701
Fragments arranged by size:
2701 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 661 4074 661
Size: 3413 957
BsaAI yAC'GTr
Cuts at: 1723 1723
Size: 4370
BsaBI GATnn'nnATC
Cuts at: 6 997 1884 3921 6
Size: 991 887 2037 455
Fragments arranged by size:
2037 991 887 455
BsaHI Gr'CG_yC
Cuts at: 1175 1801 2049 2751 4247 1175
Size: 626 248 702 1496 1298
Fragments arranged by size:
1496 1298 702 626 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 307
Size: 4370
BseYI C'CCAG_C
Cuts at: 132 172 720 784 1260 1795 2553 3130 3626
Size: 40 548 64 476 535 758 577 496
Cuts at: 3626 132
Size: 876
Fragments arranged by size:
876 758 577 548 535 496 476 64
40
BsiEI CG_ry'CG
Cuts at: 104 343 530 1912 1958 3166 104
Size: 239 187 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 239 187 46
BsiHKAI G_wGCw'C
Cuts at: 353 569 662 905 968 1097 1384 2162 2352
Size: 216 93 243 63 129 287 778 190
Cuts at: 2352 3144 353
Size: 792 1579
Fragments arranged by size:
1579 792 778 287 243 216 190 129
93 63
BsmBI CGTCTCn'nnnn_
Cuts at: 367 367
Size: 4370
Bsp1286I G_dGCh'C
Cuts at: 181 200 353 548 569 662 905 968 1097
Size: 19 153 195 21 93 243 63 129
Cuts at: 1097 1384 1995 2088 2162 2352 2414 3144 181
Size: 287 611 93 74 190 62 730 1407
Fragments arranged by size:
1407 730 611 287 243 195 190 153
129 93 93 74 63 62 21 19
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 3546 3546
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 440 1420 1634 1936 2317 440
Size: 980 214 302 381 2493
Fragments arranged by size:
2493 980 381 302 214
BsrBI CCG'CTC
Cuts at: 50 443 1070 1118 1254 2662 2716 4200 4365
Size: 393 627 48 136 1408 54 1484 165
Cuts at: 4365 50
Size: 55
Fragments arranged by size:
1484 1408 627 393 165 136 55 54
48
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 338 2368 2549 2758 338
Size: 2030 181 209 1950
Fragments arranged by size:
2030 1950 209 181
BssHII G'CGCG_C
Cuts at: 304 304
Size: 4370
BssSI C'ACGA_G
Cuts at: 346 2641 2999 346
Size: 2295 358 1717
Fragments arranged by size:
2295 1717 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1277 1747 1277
Size: 470 3900
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstXI CCAn_nnnn'nTGG
Cuts at: 387 894 387
Size: 507 3863
BstYI r'GATC_y
Cuts at: 1 848 992 1395 1862 1885 2220 2466 3478
Size: 847 144 403 467 23 335 246 1012
Cuts at: 3478 3564 3576 3922 1
Size: 86 12 346 449
Fragments arranged by size:
1012 847 467 449 403 346 335 246
144 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1040 1040
Size: 4370
BtgI C'CryG_G
Cuts at: 123 524 1361 123
Size: 401 837 3132
Fragments arranged by size:
3132 837 401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 660 795 1095 1613 660
Size: 135 300 518 3417
Fragments arranged by size:
3417 518 300 135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1062 1097 1062
Size: 35 4335
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 2076 2934 2076
Size: 858 3512
EaeI y'GGCC_r
Cuts at: 340 377 389 497 527 1955 2129 2520 2547
Size: 37 12 108 30 1428 174 391 27
Cuts at: 2547 3892 340
Size: 1345 818
Fragments arranged by size:
1428 1345 818 391 174 108 37 30
27 12
EagI C'GGCC_G
Cuts at: 340 527 1955 340
Size: 187 1428 2755
Fragments arranged by size:
2755 1428 187
EarI CTCTTCn'nnn_
Cuts at: 358 690 2393 2603 358
Size: 332 1703 210 2125
Fragments arranged by size:
2125 1703 332 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 351 1382 351
Size: 1031 3339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 1032 1375 2195 2627 3374 3642 319
Size: 713 343 820 432 747 268 1047
Fragments arranged by size:
1047 820 747 713 432 343 268
EcoNI CCTnn'n_nnAGG
Cuts at: 421 421
Size: 4370
EcoO109I rG'GnC_Cy
Cuts at: 732 1509 732
Size: 777 3593
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 492 2389 492
Size: 1897 2473
FspI TGC'GCA
Cuts at: 421 739 2151 421
Size: 318 1412 2640
Fragments arranged by size:
2640 1412 318
HaeII r_GCGC'y
Cuts at: 278 477 554 1804 2052 3074 4053 278
Size: 199 77 1250 248 1022 979 595
Fragments arranged by size:
1250 1022 979 595 248 199 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 751 783 2212 2242 2244 2274 751
Size: 32 1429 30 2 30 2847
Fragments arranged by size:
2847 1429 32 30 30 2
HincII GTy'rAC
Cuts at: 152 1409 152
Size: 1257 3113
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 1289 3803 1289
Size: 2514 1856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 749 782 1229 3041 3225 749
Size: 33 447 1812 184 1894
Fragments arranged by size:
1894 1812 447 184 33
MscI TGG'CCA
Cuts at: 391 2131 391
Size: 1740 2630
MslI CAynn'nnrTG
Cuts at: 715 2359 2486 715
Size: 1644 127 2599
Fragments arranged by size:
2599 1644 127
MspA1I CmG'CkG
Cuts at: 125 1239 1481 1795 2155 3168 3413 125
Size: 1114 242 314 360 1013 245 1082
Fragments arranged by size:
1114 1082 1013 360 314 245 242
NaeI GCC'GGC
Cuts at: 340 2551 340
Size: 2211 2159
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NgoMIV G'CCGG_C
Cuts at: 338 2549 338
Size: 2211 2159
NspI r_CATG'y
Cuts at: 150 1423 1923 2830 150
Size: 1273 500 907 1690
Fragments arranged by size:
1690 1273 907 500
PciI A'CATG_T
Cuts at: 146 2826 146
Size: 2680 1690
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PstI C_TGCA'G
Cuts at: 290 1417 2102 290
Size: 1127 685 2558
Fragments arranged by size:
2558 1127 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 1239 1795 2155 1239
Size: 556 360 3454
Fragments arranged by size:
3454 556 360
RsrII CG'GwC_CG
Cuts at: 771 2565 771
Size: 1794 2576
SacI G_AGCT'C
Cuts at: 353 1384 353
Size: 1031 3339
SacII CC_GC'GG
Cuts at: 126 126
Size: 4370
SalI G'TCGA_C
Cuts at: 150 1407 150
Size: 1257 3113
SapI GCTCTTCn'nnn_
Cuts at: 358 690 2393 2603 358
Size: 332 1703 210 2125
Fragments arranged by size:
2125 1703 332 210
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 652 3887 652
Size: 3235 1135
SfcI C'TryA_G
Cuts at: 33 286 580 1413 2098 3091 3282 33
Size: 253 294 833 685 993 191 1121
Fragments arranged by size:
1121 993 833 685 294 253 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 731 731
Size: 4370
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 184 398 1392 184
Size: 214 994 3162
Fragments arranged by size:
3162 994 214
SmlI C'TyrA_G
Cuts at: 1525 2440 2932 3194 1525
Size: 915 492 262 2701
Fragments arranged by size:
2701 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StuI AGG'CCT
Cuts at: 610 610
Size: 4370
StyI C'CwwG_G
Cuts at: 621 878 1504 1858 621
Size: 257 626 354 3133
Fragments arranged by size:
3133 626 354 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 723 788 957 1953 2086 2545 2754 723
Size: 65 169 996 133 459 209 2339
Fragments arranged by size:
2339 996 459 209 169 133 65
TatI w'GTAC_w
Cuts at: 650 3885 650
Size: 3235 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 1186 2167 1186
Size: 981 3389
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 717 1865 717
Size: 1148 3222
XmaI C'CCGG_G
Cuts at: 182 396 1390 182
Size: 214 994 3162
Fragments arranged by size:
3162 994 214
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BsaXI BsgI BsiWI
BsmI BsrGI BstEII BstZ17I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI