Restriction map for
pF1KE3145
(Circular) (Six-base) MAPSORT of: pF1KE3145.seq Check: 7407 from: 1 to: 4175
pF1KE3145 4175 bp
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AccI GT'mk_AC
Cuts at: 1213 1213
Size: 4175
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 678 2000 2432 3179 3447 160
Size: 518 1322 432 747 268 888
Fragments arranged by size:
1322 888 747 518 432 268
AflIII A'CryG_T
Cuts at: 1527 2631 3608 1527
Size: 1104 977 2094
Fragments arranged by size:
2094 1104 977
AgeI A'CCGG_T
Cuts at: 2563 2563
Size: 4175
AlwNI CAG_nnn'CTG
Cuts at: 601 866 1480 3047 601
Size: 265 614 1567 1729
Fragments arranged by size:
1729 1567 614 265
ApaLI G'TGCA_C
Cuts at: 2945 2945
Size: 4175
ApoI r'AATT_y
Cuts at: 512 1038 1061 1179 3528 3784 512
Size: 526 23 118 2349 256 903
Fragments arranged by size:
2349 903 526 256 118 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 638 1195 638
Size: 557 3618
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 1191 1605 1853 1888 1191
Size: 414 248 35 3478
Fragments arranged by size:
3478 414 248 35
BanII G_rGCy'C
Cuts at: 1189 2219 1189
Size: 1030 3145
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 821 821
Size: 4175
BbvCI CC'TCA_GC
Cuts at: 479 479
Size: 4175
BciVI GTATCCnnnnn_n'
Cuts at: 2073 2834 4175 2073
Size: 761 1341 2073
Fragments arranged by size:
2073 1341 761
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BfrBI ATG'CAT
Cuts at: 305 767 837 305
Size: 462 70 3643
Fragments arranged by size:
3643 462 70
BglII A'GATC_T
Cuts at: 721 1690 3727 721
Size: 969 2037 1169
Fragments arranged by size:
2037 1169 969
BlpI GC'TnA_GC
Cuts at: 188 1287 188
Size: 1099 3076
Bme1580I G_kGCm'C
Cuts at: 1800 1893 2949 1800
Size: 93 1056 3026
Fragments arranged by size:
3026 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 989 1387 1468 1803 3947 989
Size: 398 81 335 2144 1217
Fragments arranged by size:
2144 1217 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4175
Bpu10I CC'TnA_GC
Cuts at: 479 479
Size: 4175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2722 3020 1351
Size: 879 492 298 2506
Fragments arranged by size:
2506 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3879 3879
Size: 4175
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 1606 1854 2556 4052 1606
Size: 248 702 1496 1729
Fragments arranged by size:
1729 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1364 1885 2563 2837 2984 3395 1364
Size: 521 678 274 147 411 2144
Fragments arranged by size:
2144 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1600 2358 2935 3431 1600
Size: 758 577 496 2344
Fragments arranged by size:
2344 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 767 428
Size: 339 3836
BsiEI CG_ry'CG
Cuts at: 104 1717 1763 2971 104
Size: 1613 46 1208 1308
Fragments arranged by size:
1613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 2949 1189
Size: 778 190 792 2415
Fragments arranged by size:
2415 792 778 190
BsmI GAATG_Cn'
Cuts at: 307 839 307
Size: 532 3643
Bsp1286I G_dGCh'C
Cuts at: 1189 1800 1893 1967 2157 2219 2949 1189
Size: 611 93 74 190 62 730 2415
Fragments arranged by size:
2415 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3395 3395
Size: 4175
BspHI T'CATG_A
Cuts at: 3351 3351
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1225 1439 1741 2122 1225
Size: 214 302 381 3278
Fragments arranged by size:
3278 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2467 2521 4005 4170 50
Size: 2417 54 1484 165 55
Fragments arranged by size:
2417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 337 440 2087 337
Size: 103 1647 2425
Fragments arranged by size:
2425 1647 103
BsrFI r'CCGG_y
Cuts at: 2173 2354 2563 2173
Size: 181 209 3785
Fragments arranged by size:
3785 209 181
BssSI C'ACGA_G
Cuts at: 808 2446 2804 808
Size: 1638 358 2179
Fragments arranged by size:
2179 1638 358
BstAPI GCAn_nnn'nTGC
Cuts at: 790 1552 790
Size: 762 3413
BstBI TT'CG_AA
Cuts at: 2536 2536
Size: 4175
BstEII G'GTnAC_C
Cuts at: 939 939
Size: 4175
BstXI CCAn_nnnn'nTGG
Cuts at: 283 283
Size: 4175
BstYI r'GATC_y
Cuts at: 1 504 721 1200 1667 1690 2025 2271 3283
Size: 503 217 479 467 23 335 246 1012
Cuts at: 3283 3369 3381 3727 1
Size: 86 12 346 449
Fragments arranged by size:
1012 503 479 467 449 346 335 246
217 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 744 1418 744
Size: 674 3501
DraI TTT'AAA
Cuts at: 681 1043 1174 681
Size: 362 131 3682
Fragments arranged by size:
3682 362 131
DraIII CAC_nnn'GTG
Cuts at: 790 790
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 2739 1881
Size: 858 3317
EaeI y'GGCC_r
Cuts at: 1760 1934 2325 2352 3697 1760
Size: 174 391 27 1345 2238
Fragments arranged by size:
2238 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2383 2693 2839 3985 2383
Size: 310 146 1146 2573
Fragments arranged by size:
2573 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 184 678 2000 2432 3179 3447 160
Size: 24 494 1322 432 747 268 888
Fragments arranged by size:
1322 888 747 494 432 268 24
EcoO109I rG'GnC_Cy
Cuts at: 1314 1314
Size: 4175
EcoRI G'AATT_C
Cuts at: 512 1179 512
Size: 667 3508
EcoRV GAT'ATC
Cuts at: 924 924
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 4175
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 1609 1857 2879 3858 1609
Size: 248 1022 979 1926
Fragments arranged by size:
1926 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2017 2047 2049 2079 2017
Size: 30 2 30 4113
Fragments arranged by size:
4113 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
HindIII A'AGCT_T
Cuts at: 172 172
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3030 2846
Size: 184 3991
MscI TGG'CCA
Cuts at: 1936 1936
Size: 4175
MslI CAynn'nnrTG
Cuts at: 281 2164 2291 281
Size: 1883 127 2165
Fragments arranged by size:
2165 1883 127
MspA1I CmG'CkG
Cuts at: 1286 1600 1960 2973 3218 1286
Size: 314 360 1013 245 2243
Fragments arranged by size:
2243 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 107 107
Size: 4175
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NsiI A_TGCA'T
Cuts at: 307 769 839 307
Size: 462 70 3643
Fragments arranged by size:
3643 462 70
NspI r_CATG'y
Cuts at: 767 1228 1728 2635 767
Size: 461 500 907 2307
Fragments arranged by size:
2307 907 500 461
PciI A'CATG_T
Cuts at: 2631 2631
Size: 4175
PfoI T'CCnGG_A
Cuts at: 507 507
Size: 4175
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 4143
PstI C_TGCA'G
Cuts at: 1222 1907 1222
Size: 685 3490
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 1600 1960 1600
Size: 360 3815
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 249 3692 249
Size: 3443 732
SfcI C'TryA_G
Cuts at: 33 1218 1903 2896 3087 33
Size: 1185 685 993 191 1121
Fragments arranged by size:
1185 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 1197 1197
Size: 4175
SmlI C'TyrA_G
Cuts at: 638 1330 2245 2737 2999 638
Size: 692 915 492 262 1814
Fragments arranged by size:
1814 915 692 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
StuI AGG'CCT
Cuts at: 520 520
Size: 4175
StyI C'CwwG_G
Cuts at: 107 378 944 1309 1663 107
Size: 271 566 365 354 2619
Fragments arranged by size:
2619 566 365 354 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1108 1758 1891 2350 2559 1108
Size: 650 133 459 209 2724
Fragments arranged by size:
2724 650 459 209 133
TatI w'GTAC_w
Cuts at: 247 3690 247
Size: 3443 732
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4143
Tth111I GACn'n_nGTC
Cuts at: 802 1972 802
Size: 1170 3005
XbaI T'CTAG_A
Cuts at: 1029 1206 1029
Size: 177 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1670 1670
Size: 4175
XhoI C'TCGA_G
Cuts at: 638 638
Size: 4175
XmaI C'CCGG_G
Cuts at: 1195 1195
Size: 4175
XmnI GAAnn'nnTTC
Cuts at: 123 845 123
Size: 722 3453
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BglI BmgBI BmtI
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI