Restriction map for
pF1KE3123
(Circular) (Six-base) MAPSORT of: pF1KE3123.seq Check: 1799 from: 1 to: 4043
pF1KE3123 4043 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1081 1081
Size: 4043
Acc65I G'GTAC_C
Cuts at: 1059 1059
Size: 4043
AclI AA'CG_TT
Cuts at: 3883 3883
Size: 4043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 784 1868 2300 3047 3315 570
Size: 214 1084 432 747 268 1298
Fragments arranged by size:
1298 1084 747 432 268 214
AflIII A'CryG_T
Cuts at: 966 1395 2499 3476 966
Size: 429 1104 977 1533
Fragments arranged by size:
1533 1104 977 429
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 1348 2915 1348
Size: 1567 2476
ApaLI G'TGCA_C
Cuts at: 2813 2813
Size: 4043
ApoI r'AATT_y
Cuts at: 286 762 1047 3396 3652 286
Size: 476 285 2349 256 677
Fragments arranged by size:
2349 677 476 285 256
AseI AT'TA_AT
Cuts at: 20 682 20
Size: 662 3381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 1063 1063
Size: 4043
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1013 1046 1013
Size: 33 4010
BamHI G'GATC_C
Cuts at: 543 1068 543
Size: 525 3518
BanI G'GyrC_C
Cuts at: 1059 1473 1721 1756 1059
Size: 414 248 35 3346
Fragments arranged by size:
3346 414 248 35
BanII G_rGCy'C
Cuts at: 1057 2087 1057
Size: 1030 3013
BbeI G_GCGC'C
Cuts at: 1477 1725 1477
Size: 248 3795
BciVI GTATCCnnnnn_n'
Cuts at: 1014 1941 2702 4043 1014
Size: 927 761 1341 1014
Fragments arranged by size:
1341 1014 927 761
BclI T'GATC_A
Cuts at: 593 1563 593
Size: 970 3073
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 1155 1155
Size: 4043
Bme1580I G_kGCm'C
Cuts at: 1668 1761 2817 1668
Size: 93 1056 2894
Fragments arranged by size:
2894 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 157 1255 1336 1671 3815 157
Size: 1098 81 335 2144 385
Fragments arranged by size:
2144 1098 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 269
Size: 4043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2098 2590 2888 1219
Size: 879 492 298 2374
Fragments arranged by size:
2374 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3747 3747
Size: 4043
BsaAI yAC'GTr
Cuts at: 1396 1396
Size: 4043
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 1474 1722 2424 3920 1474
Size: 248 702 1496 1597
Fragments arranged by size:
1597 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 156 186 275 305 156
Size: 30 89 30 3894
Fragments arranged by size:
3894 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 146 146
Size: 4043
BseYI C'CCAG_C
Cuts at: 1468 2226 2803 3299 1468
Size: 758 577 496 2212
Fragments arranged by size:
2212 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1585 1631 2839 104
Size: 1481 46 1208 1308
Fragments arranged by size:
1481 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1057 1835 2025 2817 1057
Size: 778 190 792 2283
Fragments arranged by size:
2283 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1057 1668 1761 1835 2025 2087 2817 1057
Size: 611 93 74 190 62 730 2283
Fragments arranged by size:
2283 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1093 1307 1609 1990 1093
Size: 214 302 381 3146
Fragments arranged by size:
3146 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1955 1955
Size: 4043
BsrFI r'CCGG_y
Cuts at: 2041 2222 2431 2041
Size: 181 209 3653
Fragments arranged by size:
3653 209 181
BssSI C'ACGA_G
Cuts at: 2314 2672 2314
Size: 358 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 1420
Size: 4043
BstBI TT'CG_AA
Cuts at: 643 2404 643
Size: 1761 2282
BstYI r'GATC_y
Cuts at: 1 543 1068 1535 1558 1893 2139 3151 3237
Size: 542 525 467 23 335 246 1012 86
Cuts at: 3237 3249 3595 1
Size: 12 346 449
Fragments arranged by size:
1012 542 525 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4043
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1993 2174 2337 1993
Size: 181 163 3699
Fragments arranged by size:
3699 181 163
BtsI GCAGTG_nn'
Cuts at: 185 539 1286 185
Size: 354 747 2942
Fragments arranged by size:
2942 747 354
DraI TTT'AAA
Cuts at: 1035 1042 1035
Size: 7 4036
DrdI GACnn_nn'nnGTC
Cuts at: 1749 2607 1749
Size: 858 3185
EaeI y'GGCC_r
Cuts at: 240 1628 1802 2193 2220 3565 240
Size: 1388 174 391 27 1345 718
Fragments arranged by size:
1388 1345 718 391 174 27
EagI C'GGCC_G
Cuts at: 1628 1628
Size: 4043
EarI CTCTTCn'nnn_
Cuts at: 213 769 2066 2276 213
Size: 556 1297 210 1980
Fragments arranged by size:
1980 1297 556 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2251 2561 2707 3853 2251
Size: 310 146 1146 2441
Fragments arranged by size:
2441 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1055 1055
Size: 4043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 570 784 1868 2300 3047 3315 269
Size: 301 214 1084 432 747 268 997
Fragments arranged by size:
1084 997 747 432 301 268 214
EcoO109I rG'GnC_Cy
Cuts at: 942 1182 942
Size: 240 3803
EcoRI G'AATT_C
Cuts at: 1047 1047
Size: 4043
EcoRV GAT'ATC
Cuts at: 486 486
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 4043
FspI TGC'GCA
Cuts at: 1824 1824
Size: 4043
HaeII r_GCGC'y
Cuts at: 1477 1725 2747 3726 1477
Size: 248 1022 979 1794
Fragments arranged by size:
1794 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1885 1915 1917 1947 1885
Size: 30 2 30 3981
Fragments arranged by size:
3981 30 30 2
HincII GTy'rAC
Cuts at: 1082 1082
Size: 4043
KasI G'GCGC_C
Cuts at: 1473 1721 1473
Size: 248 3795
KpnI G_GTAC'C
Cuts at: 1063 1063
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 374 497 760 833 998 2714 2898 374
Size: 123 263 73 165 1716 184 1519
Fragments arranged by size:
1716 1519 263 184 165 123 73
MscI TGG'CCA
Cuts at: 1804 1804
Size: 4043
MslI CAynn'nnrTG
Cuts at: 242 971 2032 2159 242
Size: 729 1061 127 2126
Fragments arranged by size:
2126 1061 729 127
MspA1I CmG'CkG
Cuts at: 915 1154 1468 1828 2841 3086 915
Size: 239 314 360 1013 245 1872
Fragments arranged by size:
1872 1013 360 314 245 239
NaeI GCC'GGC
Cuts at: 2224 2224
Size: 4043
NarI GG'CG_CC
Cuts at: 1474 1722 1474
Size: 248 3795
NcoI C'CATG_G
Cuts at: 107 107
Size: 4043
NgoMIV G'CCGG_C
Cuts at: 2222 2222
Size: 4043
NspI r_CATG'y
Cuts at: 970 1096 1596 2503 970
Size: 126 500 907 2510
Fragments arranged by size:
2510 907 500 126
PciI A'CATG_T
Cuts at: 966 2499 966
Size: 1533 2510
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PpuMI rG'GwC_Cy
Cuts at: 942 942
Size: 4043
PstI C_TGCA'G
Cuts at: 180 1090 1775 180
Size: 910 685 2448
Fragments arranged by size:
2448 910 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 915 1468 1828 915
Size: 553 360 3130
Fragments arranged by size:
3130 553 360
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 1057 1057
Size: 4043
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
SbfI CC_TGCA'GG
Cuts at: 1090 1090
Size: 4043
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SfcI C'TryA_G
Cuts at: 33 164 176 807 881 1086 1771 2764 2955
Size: 131 12 631 74 205 685 993 191
Cuts at: 2955 33
Size: 1121
Fragments arranged by size:
1121 993 685 631 205 191 131 74
12
SfoI GGC'GCC
Cuts at: 1475 1723 1475
Size: 248 3795
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 1065 1065
Size: 4043
SmlI C'TyrA_G
Cuts at: 1198 2113 2605 2867 1198
Size: 915 492 262 2374
Fragments arranged by size:
2374 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1096 1596 1096
Size: 500 3543
SspI AAT'ATT
Cuts at: 993 993
Size: 4043
StyI C'CwwG_G
Cuts at: 107 1177 1531 107
Size: 1070 354 2619
Fragments arranged by size:
2619 1070 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1626 1759 2218 2427 1626
Size: 133 459 209 3242
Fragments arranged by size:
3242 459 209 133
TatI w'GTAC_w
Cuts at: 975 3558 975
Size: 2583 1460
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 4011
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 954 1538 954
Size: 584 3459
XmaI C'CCGG_G
Cuts at: 1063 1063
Size: 4043
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI