Restriction map for
pF1KE2805
(Circular) (Six-base) MAPSORT of: pF1KE2805.seq Check: 2968 from: 1 to: 3383
pF1KE2805 3383 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AccI GT'mk_AC
Cuts at: 421 421
Size: 3383
Acc65I G'GTAC_C
Cuts at: 399 399
Size: 3383
AclI AA'CG_TT
Cuts at: 3223 3223
Size: 3383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1640 2387 2655 1208
Size: 432 747 268 1936
Fragments arranged by size:
1936 747 432 268
AflIII A'CryG_T
Cuts at: 116 735 1839 2816 116
Size: 619 1104 977 683
Fragments arranged by size:
1104 977 683 619
AgeI A'CCGG_T
Cuts at: 1771 1771
Size: 3383
AhdI GACnn_n'nnGTC
Cuts at: 117 117
Size: 3383
AlwNI CAG_nnn'CTG
Cuts at: 688 2255 688
Size: 1567 1816
ApaLI G'TGCA_C
Cuts at: 2153 2153
Size: 3383
ApoI r'AATT_y
Cuts at: 387 2736 2992 387
Size: 2349 256 778
Fragments arranged by size:
2349 778 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3383
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3383
AvaI C'yCGr_G
Cuts at: 403 403
Size: 3383
BamHI G'GATC_C
Cuts at: 408 408
Size: 3383
BanI G'GyrC_C
Cuts at: 313 399 813 1061 1096 313
Size: 86 414 248 35 2600
Fragments arranged by size:
2600 414 248 86 35
BanII G_rGCy'C
Cuts at: 200 397 1427 200
Size: 197 1030 2156
Fragments arranged by size:
2156 1030 197
BbeI G_GCGC'C
Cuts at: 817 1065 817
Size: 248 3135
BciVI GTATCCnnnnn_n'
Cuts at: 1281 2042 3383 1281
Size: 761 1341 1281
Fragments arranged by size:
1341 1281 761
BclI T'GATC_A
Cuts at: 903 903
Size: 3383
BglII A'GATC_T
Cuts at: 898 2935 898
Size: 2037 1346
BlpI GC'TnA_GC
Cuts at: 495 495
Size: 3383
Bme1580I G_kGCm'C
Cuts at: 1008 1101 2157 1008
Size: 93 1056 2234
Fragments arranged by size:
2234 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 595 676 1011 3155 595
Size: 81 335 2144 823
Fragments arranged by size:
2144 823 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 157 1750 157
Size: 1593 1790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 559 1438 1930 2228 337
Size: 222 879 492 298 1492
Fragments arranged by size:
1492 879 492 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 3087 3087
Size: 3383
BsaAI yAC'GTr
Cuts at: 736 736
Size: 3383
BsaBI GATnn'nnATC
Cuts at: 6 897 2934 6
Size: 891 2037 455
Fragments arranged by size:
2037 891 455
BsaHI Gr'CG_yC
Cuts at: 814 1062 1764 3260 814
Size: 248 702 1496 937
Fragments arranged by size:
1496 937 702 248
BsaWI w'CCGG_w
Cuts at: 572 1093 1771 2045 2192 2603 572
Size: 521 678 274 147 411 1352
Fragments arranged by size:
1352 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 173 246 332 808 1566 2143 2639 173
Size: 73 86 476 758 577 496 917
Fragments arranged by size:
917 758 577 496 476 86 73
BsiEI CG_ry'CG
Cuts at: 104 925 971 2179 104
Size: 821 46 1208 1308
Fragments arranged by size:
1308 1208 821 46
BsiHKAI G_wGCw'C
Cuts at: 200 397 1175 1365 2157 200
Size: 197 778 190 792 1426
Fragments arranged by size:
1426 792 778 197 190
Bsp1286I G_dGCh'C
Cuts at: 200 397 1008 1101 1175 1365 1427 2157 200
Size: 197 611 93 74 190 62 730 1426
Fragments arranged by size:
1426 730 611 197 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2603 2603
Size: 3383
BspHI T'CATG_A
Cuts at: 2559 2559
Size: 3383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 433 647 949 1330 433
Size: 214 302 381 2486
Fragments arranged by size:
2486 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1675 1729 3213 3378 50
Size: 1625 54 1484 165 55
Fragments arranged by size:
1625 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1295 1295
Size: 3383
BsrFI r'CCGG_y
Cuts at: 1381 1562 1771 1381
Size: 181 209 2993
Fragments arranged by size:
2993 209 181
BssSI C'ACGA_G
Cuts at: 1654 2012 1654
Size: 358 3025
BstAPI GCAn_nnn'nTGC
Cuts at: 760 760
Size: 3383
BstBI TT'CG_AA
Cuts at: 1744 1744
Size: 3383
BstEII G'GTnAC_C
Cuts at: 213 213
Size: 3383
BstYI r'GATC_y
Cuts at: 1 408 875 898 1233 1479 2491 2577 2589
Size: 407 467 23 335 246 1012 86 12
Cuts at: 2589 2935 1
Size: 346 449
Fragments arranged by size:
1012 467 449 407 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3383
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1333 1514 1677 1333
Size: 181 163 3039
Fragments arranged by size:
3039 181 163
BtsI GCAGTG_nn'
Cuts at: 345 626 345
Size: 281 3102
DraI TTT'AAA
Cuts at: 382 382
Size: 3383
DraIII CAC_nnn'GTG
Cuts at: 323 323
Size: 3383
DrdI GACnn_nn'nnGTC
Cuts at: 1089 1947 1089
Size: 858 2525
EaeI y'GGCC_r
Cuts at: 968 1142 1533 1560 2905 968
Size: 174 391 27 1345 1446
Fragments arranged by size:
1446 1345 391 174 27
EagI C'GGCC_G
Cuts at: 968 968
Size: 3383
EarI CTCTTCn'nnn_
Cuts at: 1406 1616 1406
Size: 210 3173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1591 1901 2047 3193 1591
Size: 310 146 1146 1781
Fragments arranged by size:
1781 1146 310 146
EcoICRI GAG'CTC
Cuts at: 198 395 198
Size: 197 3186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1640 2387 2655 1208
Size: 432 747 268 1936
Fragments arranged by size:
1936 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 522 522
Size: 3383
EcoRI G'AATT_C
Cuts at: 387 387
Size: 3383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1402 1402
Size: 3383
FspI TGC'GCA
Cuts at: 1164 1164
Size: 3383
HaeII r_GCGC'y
Cuts at: 817 1065 2087 3066 817
Size: 248 1022 979 1134
Fragments arranged by size:
1134 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 1225 1255 1257 1287 103
Size: 32 1090 30 2 30 2199
Fragments arranged by size:
2199 1090 32 30 30 2
HincII GTy'rAC
Cuts at: 422 422
Size: 3383
KasI G'GCGC_C
Cuts at: 813 1061 813
Size: 248 3135
KpnI G_GTAC'C
Cuts at: 403 403
Size: 3383
MluI A'CGCG_T
Cuts at: 2816 2816
Size: 3383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2238 2054
Size: 184 3199
MscI TGG'CCA
Cuts at: 1144 1144
Size: 3383
MslI CAynn'nnrTG
Cuts at: 272 1372 1499 272
Size: 1100 127 2156
Fragments arranged by size:
2156 1100 127
MspA1I CmG'CkG
Cuts at: 494 808 1168 2181 2426 494
Size: 314 360 1013 245 1451
Fragments arranged by size:
1451 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1564 1564
Size: 3383
NarI GG'CG_CC
Cuts at: 814 1062 814
Size: 248 3135
NcoI C'CATG_G
Cuts at: 107 107
Size: 3383
NgoMIV G'CCGG_C
Cuts at: 1562 1562
Size: 3383
NruI TCG'CGA
Cuts at: 202 202
Size: 3383
NspI r_CATG'y
Cuts at: 120 436 936 1843 120
Size: 316 500 907 1660
Fragments arranged by size:
1660 907 500 316
PciI A'CATG_T
Cuts at: 116 1839 116
Size: 1723 1660
PmeI GTTT'AAAC
Cuts at: 382 382
Size: 3383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2522 2554 2522
Size: 32 3351
PstI C_TGCA'G
Cuts at: 430 1115 430
Size: 685 2698
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3383
PvuII CAG'CTG
Cuts at: 808 1168 808
Size: 360 3023
RsrII CG'GwC_CG
Cuts at: 1578 1578
Size: 3383
SacI G_AGCT'C
Cuts at: 200 397 200
Size: 197 3186
SalI G'TCGA_C
Cuts at: 420 420
Size: 3383
SapI GCTCTTCn'nnn_
Cuts at: 1406 1616 1406
Size: 210 3173
SbfI CC_TGCA'GG
Cuts at: 430 430
Size: 3383
ScaI AGT'ACT
Cuts at: 2900 2900
Size: 3383
SfcI C'TryA_G
Cuts at: 33 426 1111 2104 2295 33
Size: 393 685 993 191 1121
Fragments arranged by size:
1121 993 685 393 191
SfoI GGC'GCC
Cuts at: 815 1063 815
Size: 248 3135
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3383
SmaI CCC'GGG
Cuts at: 405 405
Size: 3383
SmlI C'TyrA_G
Cuts at: 352 538 1453 1945 2207 352
Size: 186 915 492 262 1528
Fragments arranged by size:
1528 915 492 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3383
SphI G_CATG'C
Cuts at: 436 936 436
Size: 500 2883
StuI AGG'CCT
Cuts at: 235 235
Size: 3383
StyI C'CwwG_G
Cuts at: 107 517 871 107
Size: 410 354 2619
Fragments arranged by size:
2619 410 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 165 966 1099 1558 1767 165
Size: 801 133 459 209 1781
Fragments arranged by size:
1781 801 459 209 133
TatI w'GTAC_w
Cuts at: 2898 2898
Size: 3383
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3351
Tth111I GACn'n_nGTC
Cuts at: 1180 1180
Size: 3383
XbaI T'CTAG_A
Cuts at: 414 414
Size: 3383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 878 878
Size: 3383
XmaI C'CCGG_G
Cuts at: 403 403
Size: 3383
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI