Restriction map for
pF1KE2783
(Circular) (Six-base) MAPSORT of: pF1KE2783.seq Check: 4962 from: 1 to: 3893
pF1KE2783 3893 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 870 870
Size: 3893
AccI GT'mk_AC
Cuts at: 380 931 380
Size: 551 3342
Acc65I G'GTAC_C
Cuts at: 909 909
Size: 3893
AclI AA'CG_TT
Cuts at: 3733 3733
Size: 3893
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1718 2150 2897 3165 235
Size: 1483 432 747 268 963
Fragments arranged by size:
1483 963 747 432 268
AflIII A'CryG_T
Cuts at: 455 1245 2349 3326 455
Size: 790 1104 977 1022
Fragments arranged by size:
1104 1022 977 790
AgeI A'CCGG_T
Cuts at: 2281 2281
Size: 3893
AleI CACnn'nnGTG
Cuts at: 374 374
Size: 3893
AlwNI CAG_nnn'CTG
Cuts at: 213 488 1198 2765 213
Size: 275 710 1567 1341
Fragments arranged by size:
1567 1341 710 275
ApaI G_GGCC'C
Cuts at: 198 198
Size: 3893
ApaLI G'TGCA_C
Cuts at: 2663 2663
Size: 3893
ApoI r'AATT_y
Cuts at: 897 3246 3502 897
Size: 2349 256 1288
Fragments arranged by size:
2349 1288 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3893
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
AvaI C'yCGr_G
Cuts at: 165 191 289 793 913 165
Size: 26 98 504 120 3145
Fragments arranged by size:
3145 504 120 98 26
BamHI G'GATC_C
Cuts at: 918 918
Size: 3893
BanI G'GyrC_C
Cuts at: 909 1323 1571 1606 909
Size: 414 248 35 3196
Fragments arranged by size:
3196 414 248 35
BanII G_rGCy'C
Cuts at: 198 907 1937 198
Size: 709 1030 2154
Fragments arranged by size:
2154 1030 709
BbeI G_GCGC'C
Cuts at: 1327 1575 1327
Size: 248 3645
BbvCI CC'TCA_GC
Cuts at: 481 826 481
Size: 345 3548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 640 671 674 705 640
Size: 31 3 31 3828
Fragments arranged by size:
3828 31 31 3
BciVI GTATCCnnnnn_n'
Cuts at: 1791 2552 3893 1791
Size: 761 1341 1791
Fragments arranged by size:
1791 1341 761
BclI T'GATC_A
Cuts at: 1413 1413
Size: 3893
BglII A'GATC_T
Cuts at: 1408 3445 1408
Size: 2037 1856
BlpI GC'TnA_GC
Cuts at: 1005 1005
Size: 3893
Bme1580I G_kGCm'C
Cuts at: 198 542 1518 1611 2667 198
Size: 344 976 93 1056 1424
Fragments arranged by size:
1424 1056 976 344 93
BmrI ACTGGGnnnn_n'
Cuts at: 1105 1186 1521 3665 1105
Size: 81 335 2144 1333
Fragments arranged by size:
2144 1333 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 3893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 683
Size: 3893
Bpu10I CC'TnA_GC
Cuts at: 481 826 481
Size: 345 3548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1948 2440 2738 1069
Size: 879 492 298 2224
Fragments arranged by size:
2224 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3597 3597
Size: 3893
BsaAI yAC'GTr
Cuts at: 689 1246 689
Size: 557 3336
BsaBI GATnn'nnATC
Cuts at: 6 1407 3444 6
Size: 1401 2037 455
Fragments arranged by size:
2037 1401 455
BsaHI Gr'CG_yC
Cuts at: 1324 1572 2274 3770 1324
Size: 248 702 1496 1447
Fragments arranged by size:
1496 1447 702 248
BsaWI w'CCGG_w
Cuts at: 1082 1603 2281 2555 2702 3113 1082
Size: 521 678 274 147 411 1862
Fragments arranged by size:
1862 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 325 355 325
Size: 30 3863
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 268
Size: 3893
BseYI C'CCAG_C
Cuts at: 1318 2076 2653 3149 1318
Size: 758 577 496 2062
Fragments arranged by size:
2062 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 474 299
Size: 175 3718
BsiEI CG_ry'CG
Cuts at: 104 141 185 1435 1481 2689 104
Size: 37 44 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46 44 37
BsiHKAI G_wGCw'C
Cuts at: 907 1685 1875 2667 907
Size: 778 190 792 2133
Fragments arranged by size:
2133 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 198 542 907 1518 1611 1685 1875 1937 2667
Size: 344 365 611 93 74 190 62 730
Cuts at: 2667 198
Size: 1424
Fragments arranged by size:
1424 730 611 365 344 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3113 3113
Size: 3893
BspHI T'CATG_A
Cuts at: 3069 3069
Size: 3893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 870 943 1157 1459 1840 870
Size: 73 214 302 381 2923
Fragments arranged by size:
2923 381 302 214 73
BsrBI CCG'CTC
Cuts at: 50 129 522 2185 2239 3723 3888 50
Size: 79 393 1663 54 1484 165 55
Fragments arranged by size:
1663 1484 393 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 1805 1805
Size: 3893
BsrFI r'CCGG_y
Cuts at: 140 1891 2072 2281 140
Size: 1751 181 209 1752
Fragments arranged by size:
1752 1751 209 181
BssSI C'ACGA_G
Cuts at: 2164 2522 2164
Size: 358 3535
BstAPI GCAn_nnn'nTGC
Cuts at: 1270 1270
Size: 3893
BstBI TT'CG_AA
Cuts at: 2254 2254
Size: 3893
BstEII G'GTnAC_C
Cuts at: 882 882
Size: 3893
BstYI r'GATC_y
Cuts at: 1 918 1385 1408 1743 1989 3001 3087 3099
Size: 917 467 23 335 246 1012 86 12
Cuts at: 3099 3445 1
Size: 346 449
Fragments arranged by size:
1012 917 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 185 872 107
Size: 78 687 3128
Fragments arranged by size:
3128 687 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1843 2024 2187 1843
Size: 181 163 3549
Fragments arranged by size:
3549 181 163
BtsI GCAGTG_nn'
Cuts at: 626 1136 626
Size: 510 3383
DraI TTT'AAA
Cuts at: 892 892
Size: 3893
DraIII CAC_nnn'GTG
Cuts at: 576 576
Size: 3893
DrdI GACnn_nn'nnGTC
Cuts at: 1599 2457 1599
Size: 858 3035
EaeI y'GGCC_r
Cuts at: 110 182 567 803 1478 1652 2043 2070 3415
Size: 72 385 236 675 174 391 27 1345
Cuts at: 3415 110
Size: 588
Fragments arranged by size:
1345 675 588 391 385 236 174 72
27
EagI C'GGCC_G
Cuts at: 182 1478 182
Size: 1296 2597
EarI CTCTTCn'nnn_
Cuts at: 323 1916 2126 323
Size: 1593 210 2090
Fragments arranged by size:
2090 1593 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2101 2411 2557 3703 2101
Size: 310 146 1146 2291
Fragments arranged by size:
2291 1146 310 146
EcoICRI GAG'CTC
Cuts at: 905 905
Size: 3893
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 683 1718 2150 2897 3165 235
Size: 448 1035 432 747 268 963
Fragments arranged by size:
1035 963 747 448 432 268
EcoO109I rG'GnC_Cy
Cuts at: 161 195 744 1032 161
Size: 34 549 288 3022
Fragments arranged by size:
3022 549 288 34
EcoRI G'AATT_C
Cuts at: 897 897
Size: 3893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1912 1912
Size: 3893
FspI TGC'GCA
Cuts at: 1674 1674
Size: 3893
HaeII r_GCGC'y
Cuts at: 151 1327 1575 2597 3576 151
Size: 1176 248 1022 979 468
Fragments arranged by size:
1176 1022 979 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 1735 1765 1767 1797 325
Size: 32 1378 30 2 30 2421
Fragments arranged by size:
2421 1378 32 30 30 2
HincII GTy'rAC
Cuts at: 932 932
Size: 3893
KasI G'GCGC_C
Cuts at: 1323 1571 1323
Size: 248 3645
KpnI G_GTAC'C
Cuts at: 913 913
Size: 3893
MluI A'CGCG_T
Cuts at: 3326 3326
Size: 3893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 427 2564 2748 427
Size: 2137 184 1572
Fragments arranged by size:
2137 1572 184
MscI TGG'CCA
Cuts at: 112 569 1654 112
Size: 457 1085 2351
Fragments arranged by size:
2351 1085 457
MslI CAynn'nnrTG
Cuts at: 374 862 1882 2009 374
Size: 488 1020 127 2258
Fragments arranged by size:
2258 1020 488 127
MspA1I CmG'CkG
Cuts at: 374 760 1004 1318 1678 2691 2936 374
Size: 386 244 314 360 1013 245 1331
Fragments arranged by size:
1331 1013 386 360 314 245 244
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 3893
NarI GG'CG_CC
Cuts at: 1324 1572 1324
Size: 248 3645
NcoI C'CATG_G
Cuts at: 107 107
Size: 3893
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 3893
NspI r_CATG'y
Cuts at: 459 564 946 1446 2353 459
Size: 105 382 500 907 1999
Fragments arranged by size:
1999 907 500 382 105
PciI A'CATG_T
Cuts at: 455 2349 455
Size: 1894 1999
PflMI CCAn_nnn'nTGG
Cuts at: 446 446
Size: 3893
PmeI GTTT'AAAC
Cuts at: 892 892
Size: 3893
PmlI CAC'GTG
Cuts at: 689 689
Size: 3893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 3861
PpuMI rG'GwC_Cy
Cuts at: 744 744
Size: 3893
PspOMI G'GGCC_C
Cuts at: 194 194
Size: 3893
PstI C_TGCA'G
Cuts at: 940 1625 940
Size: 685 3208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3893
PvuII CAG'CTG
Cuts at: 760 1318 1678 760
Size: 558 360 2975
Fragments arranged by size:
2975 558 360
RsrII CG'GwC_CG
Cuts at: 2088 2088
Size: 3893
SacI G_AGCT'C
Cuts at: 907 907
Size: 3893
SalI G'TCGA_C
Cuts at: 930 930
Size: 3893
SapI GCTCTTCn'nnn_
Cuts at: 1916 2126 1916
Size: 210 3683
SbfI CC_TGCA'GG
Cuts at: 940 940
Size: 3893
ScaI AGT'ACT
Cuts at: 3410 3410
Size: 3893
SfcI C'TryA_G
Cuts at: 33 306 603 936 1621 2614 2805 33
Size: 273 297 333 685 993 191 1121
Fragments arranged by size:
1121 993 685 333 297 273 191
SfoI GGC'GCC
Cuts at: 1325 1573 1325
Size: 248 3645
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3893
SmaI CCC'GGG
Cuts at: 193 291 915 193
Size: 98 624 3171
Fragments arranged by size:
3171 624 98
SmlI C'TyrA_G
Cuts at: 1048 1963 2455 2717 1048
Size: 915 492 262 2224
Fragments arranged by size:
2224 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3893
SphI G_CATG'C
Cuts at: 946 1446 946
Size: 500 3393
SrfI GCCC'GGGC
Cuts at: 193 193
Size: 3893
StyI C'CwwG_G
Cuts at: 107 1027 1381 107
Size: 920 354 2619
Fragments arranged by size:
2619 920 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1476 1609 2068 2277 1476
Size: 133 459 209 3092
Fragments arranged by size:
3092 459 209 133
TatI w'GTAC_w
Cuts at: 3408 3408
Size: 3893
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 203 235 3410 3442 203
Size: 32 3175 32 654
Fragments arranged by size:
3175 654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1690 1690
Size: 3893
XbaI T'CTAG_A
Cuts at: 924 924
Size: 3893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 1388 120
Size: 1268 2625
XmaI C'CCGG_G
Cuts at: 191 289 913 191
Size: 98 624 3171
Fragments arranged by size:
3171 624 98
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BglI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI ZraI