Restriction map for
pF1KE2770
(Circular) (Six-base) MAPSORT of: pF1KE2770.seq Check: 9255 from: 1 to: 6767
pF1KE2770 6767 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 3182 3805 3182
Size: 623 6144
Acc65I G'GTAC_C
Cuts at: 3783 3783
Size: 6767
AclI AA'CG_TT
Cuts at: 6607 6607
Size: 6767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1162 1228 1393 4592 5024 5771 6039 1162
Size: 66 165 3199 432 747 268 1890
Fragments arranged by size:
3199 1890 747 432 268 165 66
AflII C'TTAA_G
Cuts at: 2773 2773
Size: 6767
AflIII A'CryG_T
Cuts at: 305 4119 5223 6200 305
Size: 3814 1104 977 872
Fragments arranged by size:
3814 1104 977 872
AgeI A'CCGG_T
Cuts at: 5155 5155
Size: 6767
AleI CACnn'nnGTG
Cuts at: 455 3667 455
Size: 3212 3555
AlwNI CAG_nnn'CTG
Cuts at: 603 3148 3193 4072 5639 603
Size: 2545 45 879 1567 1731
Fragments arranged by size:
2545 1731 1567 879 45
ApaI G_GGCC'C
Cuts at: 1723 1848 2263 1723
Size: 125 415 6227
Fragments arranged by size:
6227 415 125
ApaLI G'TGCA_C
Cuts at: 3672 3709 5537 3672
Size: 37 1828 4902
Fragments arranged by size:
4902 1828 37
ApoI r'AATT_y
Cuts at: 1368 3208 3643 3771 6120 6376 1368
Size: 1840 435 128 2349 256 1759
Fragments arranged by size:
2349 1840 1759 435 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 6767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6767
AvaI C'yCGr_G
Cuts at: 1841 1967 2030 2081 2458 3787 1841
Size: 126 63 51 377 1329 4821
Fragments arranged by size:
4821 1329 377 126 63 51
BamHI G'GATC_C
Cuts at: 3792 3792
Size: 6767
BanI G'GyrC_C
Cuts at: 1748 3396 3783 4197 4445 4480 1748
Size: 1648 387 414 248 35 4035
Fragments arranged by size:
4035 1648 414 387 248 35
BanII G_rGCy'C
Cuts at: 260 1619 1723 1739 1848 1910 2152 2263 2539
Size: 1359 104 16 109 62 242 111 276
Cuts at: 2539 2896 3781 4811 260
Size: 357 885 1030 2216
Fragments arranged by size:
2216 1359 1030 885 357 276 242 111
109 104 62 16
BbeI G_GCGC'C
Cuts at: 4201 4449 4201
Size: 248 6519
BbsI GAAGACnn'nnnn_
Cuts at: 1406 2071 1406
Size: 665 6102
BciVI GTATCCnnnnn_n'
Cuts at: 2438 4665 5426 6767 2438
Size: 2227 761 1341 2438
Fragments arranged by size:
2438 2227 1341 761
BclI T'GATC_A
Cuts at: 4287 4287
Size: 6767
BfrBI ATG'CAT
Cuts at: 3281 3410 3281
Size: 129 6638
BglI GCCn_nnn'nGGC
Cuts at: 1743 1843 2688 1743
Size: 100 845 5822
Fragments arranged by size:
5822 845 100
BglII A'GATC_T
Cuts at: 3198 4282 6319 3198
Size: 1084 2037 3646
Fragments arranged by size:
3646 2037 1084
BlpI GC'TnA_GC
Cuts at: 1268 3658 3879 1268
Size: 2390 221 4156
Fragments arranged by size:
4156 2390 221
Bme1580I G_kGCm'C
Cuts at: 1723 1848 2120 2263 3676 3713 4392 4485 5541
Size: 125 272 143 1413 37 679 93 1056
Cuts at: 5541 1723
Size: 2949
Fragments arranged by size:
2949 1413 1056 679 272 143 125 93
37
BmrI ACTGGGnnnn_n'
Cuts at: 742 3474 3529 3979 4060 4395 6539 742
Size: 2732 55 450 81 335 2144 970
Fragments arranged by size:
2732 2144 970 450 335 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5134 5134
Size: 6767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1812 2109 2635 2670 2880 692
Size: 1120 297 526 35 210 4579
Fragments arranged by size:
4579 1120 526 297 210 35
Bpu10I CC'TnA_GC
Cuts at: 1643 2222 2383 1643
Size: 579 161 6027
Fragments arranged by size:
6027 579 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 2532 2978 3943 4822 5314 5612 890
Size: 1642 446 965 879 492 298 2045
Fragments arranged by size:
2045 1642 965 879 492 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 671 892 2027 6471 671
Size: 221 1135 4444 967
Fragments arranged by size:
4444 1135 967 221
BsaAI yAC'GTr
Cuts at: 4120 4120
Size: 6767
BsaBI GATnn'nnATC
Cuts at: 6 4281 6318 6
Size: 4275 2037 455
Fragments arranged by size:
4275 2037 455
BsaHI Gr'CG_yC
Cuts at: 4198 4446 5148 6644 4198
Size: 248 702 1496 4321
Fragments arranged by size:
4321 1496 702 248
BsaWI w'CCGG_w
Cuts at: 588 1635 2926 3956 4477 5155 5429 5576 5987
Size: 1047 1291 1030 521 678 274 147 411
Cuts at: 5987 588
Size: 1368
Fragments arranged by size:
1368 1291 1047 1030 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1617 1647 2134 2164 1617
Size: 30 487 30 6220
Fragments arranged by size:
6220 487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1455 1890 2384 2547 2685 2895 1455
Size: 435 494 163 138 210 5327
Fragments arranged by size:
5327 494 435 210 163 138
BseYI C'CCAG_C
Cuts at: 852 929 1629 2450 4192 4950 5527 6023 852
Size: 77 700 821 1742 758 577 496 1596
Fragments arranged by size:
1742 1596 821 758 700 577 496 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1553 2753 1457
Size: 96 1200 5471
Fragments arranged by size:
5471 1200 96
BsiEI CG_ry'CG
Cuts at: 104 4309 4355 5563 104
Size: 4205 46 1208 1308
Fragments arranged by size:
4205 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1488 1732 2152 2228 2539 2915 3676 3713 3781
Size: 244 420 76 311 376 761 37 68
Cuts at: 3781 4559 4749 5541 1488
Size: 778 190 792 2714
Fragments arranged by size:
2714 792 778 761 420 376 311 244
190 76 68 37
BsmI GAATG_Cn'
Cuts at: 431 1794 431
Size: 1363 5404
Bsp1286I G_dGCh'C
Cuts at: 260 1488 1619 1723 1732 1739 1848 1910 2120
Size: 1228 131 104 9 7 109 62 210
Cuts at: 2120 2152 2228 2263 2539 2896 2915 3676 3713
Size: 32 76 35 276 357 19 761 37
Cuts at: 3713 3781 4392 4485 4559 4749 4811 5541 260
Size: 68 611 93 74 190 62 730 1486
Fragments arranged by size:
1486 1228 761 730 611 357 276 210
190 131 109 104 93 76 74 68
62 62 37 35 32 19 9 7
BspEI T'CCGG_A
Cuts at: 1635 2926 5987 1635
Size: 1291 3061 2415
Fragments arranged by size:
3061 2415 1291
BspHI T'CATG_A
Cuts at: 5943 5943
Size: 6767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 1963 2441 3817 4031 4333 4714 176
Size: 1787 478 1376 214 302 381 2229
Fragments arranged by size:
2229 1787 1376 478 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2382 5059 5113 6597 6762 50
Size: 2332 2677 54 1484 165 55
Fragments arranged by size:
2677 2332 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 989 2347 3410 4679 989
Size: 1358 1063 1269 3077
Fragments arranged by size:
3077 1358 1269 1063
BsrFI r'CCGG_y
Cuts at: 4765 4946 5155 4765
Size: 181 209 6377
Fragments arranged by size:
6377 209 181
BsrGI T'GTAC_A
Cuts at: 149 149
Size: 6767
BssSI C'ACGA_G
Cuts at: 1782 3627 5038 5396 1782
Size: 1845 1411 358 3153
Fragments arranged by size:
3153 1845 1411 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3287 4144 3287
Size: 857 5910
BstBI TT'CG_AA
Cuts at: 174 5128 174
Size: 4954 1813
BstEII G'GTnAC_C
Cuts at: 658 658
Size: 6767
BstYI r'GATC_y
Cuts at: 1 1809 3198 3792 4259 4282 4617 4863 5875
Size: 1808 1389 594 467 23 335 246 1012
Cuts at: 5875 5961 5973 6319 1
Size: 86 12 346 449
Fragments arranged by size:
1808 1389 1012 594 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 998 1016 1741 2309 107
Size: 891 18 725 568 4565
Fragments arranged by size:
4565 891 725 568 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 346 3325 4717 4898 5061 346
Size: 2979 1392 181 163 2052
Fragments arranged by size:
2979 2052 1392 181 163
BtsI GCAGTG_nn'
Cuts at: 141 884 1063 1536 2120 3501 3513 4010 141
Size: 743 179 473 584 1381 12 497 2898
Fragments arranged by size:
2898 1381 743 584 497 473 179 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 117 152 2390 2425 117
Size: 35 2238 35 4459
Fragments arranged by size:
4459 2238 35 35
DraI TTT'AAA
Cuts at: 3766 3766
Size: 6767
DrdI GACnn_nn'nnGTC
Cuts at: 4473 5331 4473
Size: 858 5909
EaeI y'GGCC_r
Cuts at: 1228 2558 2805 4352 4526 4917 4944 6289 1228
Size: 1330 247 1547 174 391 27 1345 1706
Fragments arranged by size:
1706 1547 1345 1330 391 247 174 27
EagI C'GGCC_G
Cuts at: 4352 4352
Size: 6767
EarI CTCTTCn'nnn_
Cuts at: 660 794 1193 2524 2862 2871 4790 5000 660
Size: 134 399 1331 338 9 1919 210 2427
Fragments arranged by size:
2427 1919 1331 399 338 210 134 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4975 5285 5431 6577 4975
Size: 310 146 1146 5165
Fragments arranged by size:
5165 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2150 2537 3779 2150
Size: 387 1242 5138
Fragments arranged by size:
5138 1242 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1162 1228 1393 1812 2109 2635 2670 2880
Size: 470 66 165 419 297 526 35 210
Cuts at: 2880 4592 5024 5771 6039 692
Size: 1712 432 747 268 1420
Fragments arranged by size:
1712 1420 747 526 470 432 419 297
268 210 165 66 35
EcoNI CCTnn'n_nnAGG
Cuts at: 419 607 622 419
Size: 188 15 6564
Fragments arranged by size:
6564 188 15
EcoO109I rG'GnC_Cy
Cuts at: 870 1035 1719 1720 1845 2216 2259 2690 2811
Size: 165 684 1 125 371 43 431 121
Cuts at: 2811 3476 3621 3906 870
Size: 665 145 285 3731
Fragments arranged by size:
3731 684 665 431 371 285 165 145
125 121 43 1
EcoRI G'AATT_C
Cuts at: 3643 3771 3643
Size: 128 6639
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1423 3040 4786 1423
Size: 1617 1746 3404
Fragments arranged by size:
3404 1746 1617
FspI TGC'GCA
Cuts at: 4548 4548
Size: 6767
HaeII r_GCGC'y
Cuts at: 3250 4201 4449 5471 6450 3250
Size: 951 248 1022 979 3567
Fragments arranged by size:
3567 1022 979 951 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 597 629 1137 1169 1552 1584 1866
Size: 32 294 32 508 32 383 32 282
Cuts at: 1866 1898 2422 2454 4609 4639 4641 4671 271
Size: 32 524 32 2155 30 2 30 2367
Fragments arranged by size:
2367 2155 524 508 383 294 282 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 129 3806 129
Size: 3677 3090
HindIII A'AGCT_T
Cuts at: 2406 2406
Size: 6767
KasI G'GCGC_C
Cuts at: 4197 4445 4197
Size: 248 6519
KpnI G_GTAC'C
Cuts at: 3787 3787
Size: 6767
MluI A'CGCG_T
Cuts at: 6200 6200
Size: 6767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 800 1675 1908 3063 5438 5622 800
Size: 875 233 1155 2375 184 1945
Fragments arranged by size:
2375 1945 1155 875 233 184
MscI TGG'CCA
Cuts at: 2560 2807 4528 2560
Size: 247 1721 4799
Fragments arranged by size:
4799 1721 247
MslI CAynn'nnrTG
Cuts at: 455 3310 3667 4756 4883 455
Size: 2855 357 1089 127 2339
Fragments arranged by size:
2855 2339 1089 357 127
MspA1I CmG'CkG
Cuts at: 1267 1671 1705 2279 3878 4192 4552 5565 5810
Size: 404 34 574 1599 314 360 1013 245
Cuts at: 5810 1267
Size: 2224
Fragments arranged by size:
2224 1599 1013 574 404 360 314 245
34
NaeI GCC'GGC
Cuts at: 4948 4948
Size: 6767
NarI GG'CG_CC
Cuts at: 4198 4446 4198
Size: 248 6519
NcoI C'CATG_G
Cuts at: 107 1016 107
Size: 909 5858
NgoMIV G'CCGG_C
Cuts at: 4946 4946
Size: 6767
NsiI A_TGCA'T
Cuts at: 3283 3412 3283
Size: 129 6638
NspI r_CATG'y
Cuts at: 309 1312 3285 3820 4320 5227 309
Size: 1003 1973 535 500 907 1849
Fragments arranged by size:
1973 1849 1003 907 535 500
PciI A'CATG_T
Cuts at: 305 5223 305
Size: 4918 1849
PflMI CCAn_nnn'nTGG
Cuts at: 2310 2310
Size: 6767
PfoI T'CCnGG_A
Cuts at: 2318 2318
Size: 6767
PmeI GTTT'AAAC
Cuts at: 3766 3766
Size: 6767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5906 5938 5906
Size: 32 6735
PpuMI rG'GwC_Cy
Cuts at: 870 2216 2811 3476 870
Size: 1346 595 665 4161
Fragments arranged by size:
4161 1346 665 595
PshAI GACnn'nnGTC
Cuts at: 1690 1690
Size: 6767
PspOMI G'GGCC_C
Cuts at: 1719 1844 2259 1719
Size: 125 415 6227
Fragments arranged by size:
6227 415 125
PstI C_TGCA'G
Cuts at: 1994 3814 4499 1994
Size: 1820 685 4262
Fragments arranged by size:
4262 1820 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6767
PvuII CAG'CTG
Cuts at: 1267 1671 1705 4192 4552 1267
Size: 404 34 2487 360 3482
Fragments arranged by size:
3482 2487 404 360 34
RsrII CG'GwC_CG
Cuts at: 4962 4962
Size: 6767
SacI G_AGCT'C
Cuts at: 2152 2539 3781 2152
Size: 387 1242 5138
Fragments arranged by size:
5138 1242 387
SalI G'TCGA_C
Cuts at: 3804 3804
Size: 6767
SanDI GG'GwC_CC
Cuts at: 3476 3476
Size: 6767
SapI GCTCTTCn'nnn_
Cuts at: 660 4790 5000 660
Size: 4130 210 2427
Fragments arranged by size:
4130 2427 210
SbfI CC_TGCA'GG
Cuts at: 1994 3814 1994
Size: 1820 4947
ScaI AGT'ACT
Cuts at: 345 6284 345
Size: 5939 828
SexAI A'CCwGG_T
Cuts at: 3704 3704
Size: 6767
SfcI C'TryA_G
Cuts at: 33 1990 3194 3810 4495 5488 5679 33
Size: 1957 1204 616 685 993 191 1121
Fragments arranged by size:
1957 1204 1121 993 685 616 191
SfoI GGC'GCC
Cuts at: 4199 4447 4199
Size: 248 6519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6767
SmaI CCC'GGG
Cuts at: 1843 1969 2083 2460 3789 1843
Size: 126 114 377 1329 4821
Fragments arranged by size:
4821 1329 377 126 114
SmlI C'TyrA_G
Cuts at: 905 2547 2773 2993 3922 4837 5329 5591 905
Size: 1642 226 220 929 915 492 262 2081
Fragments arranged by size:
2081 1642 929 915 492 262 226 220
SpeI A'CTAG_T
Cuts at: 67 454 2290 67
Size: 387 1836 4544
Fragments arranged by size:
4544 1836 387
SphI G_CATG'C
Cuts at: 3285 3820 4320 3285
Size: 535 500 5732
Fragments arranged by size:
5732 535 500
SrfI GCCC'GGGC
Cuts at: 1969 1969
Size: 6767
SspI AAT'ATT
Cuts at: 3266 3266
Size: 6767
StuI AGG'CCT
Cuts at: 1960 1960
Size: 6767
StyI C'CwwG_G
Cuts at: 107 618 866 1016 2057 3677 3901 4255 107
Size: 511 248 150 1041 1620 224 354 2619
Fragments arranged by size:
2619 1620 1041 511 354 248 224 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 677 1766 4350 4483 4942 5151 677
Size: 1089 2584 133 459 209 2293
Fragments arranged by size:
2584 2293 1089 459 209 133
TatI w'GTAC_w
Cuts at: 149 343 1261 6282 149
Size: 194 918 5021 634
Fragments arranged by size:
5021 918 634 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 454 486 2134 2166 6284 6316 454
Size: 32 1648 32 4118 32 905
Fragments arranged by size:
4118 1648 905 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4564 4564
Size: 6767
XbaI T'CTAG_A
Cuts at: 3798 3798
Size: 6767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 616 3303 3487 4262 616
Size: 2687 184 775 3121
Fragments arranged by size:
3121 2687 775 184
XmaI C'CCGG_G
Cuts at: 1841 1967 2081 2458 3787 1841
Size: 126 114 377 1329 4821
Fragments arranged by size:
4821 1329 377 126 114
XmnI GAAnn'nnTTC
Cuts at: 239 334 1202 239
Size: 95 868 5804
Fragments arranged by size:
5804 868 95
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BsiWI BsmBI BssHII
BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PmlI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI
SwaI XhoI ZraI