Restriction map for
pF1KE2764
(Circular) (Six-base) MAPSORT of: pF1KE2764.seq Check: 2945 from: 1 to: 4685
pF1KE2764 4685 bp
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 1723 1723
Size: 4685
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4685
AclI AA'CG_TT
Cuts at: 1679 4525 1679
Size: 2846 1839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 2510 2942 3689 3957 358
Size: 2152 432 747 268 1086
Fragments arranged by size:
2152 1086 747 432 268
AflIII A'CryG_T
Cuts at: 410 780 1193 2037 3141 4118 410
Size: 370 413 844 1104 977 977
Fragments arranged by size:
1104 977 977 844 413 370
AgeI A'CCGG_T
Cuts at: 3073 3073
Size: 4685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 219 1276 1300 1450 1656 219
Size: 1057 24 150 206 3248
Fragments arranged by size:
3248 1057 206 150 24
AlwNI CAG_nnn'CTG
Cuts at: 951 1990 3557 951
Size: 1039 1567 2079
Fragments arranged by size:
2079 1567 1039
ApaI G_GGCC'C
Cuts at: 811 1603 811
Size: 792 3893
ApaLI G'TGCA_C
Cuts at: 3455 3455
Size: 4685
ApoI r'AATT_y
Cuts at: 1063 1689 4038 4294 1063
Size: 626 2349 256 1454
Fragments arranged by size:
2349 1454 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4685
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
AvaI C'yCGr_G
Cuts at: 1705 1705
Size: 4685
BamHI G'GATC_C
Cuts at: 1518 1710 1518
Size: 192 4493
BanI G'GyrC_C
Cuts at: 203 1613 1701 2115 2363 2398 203
Size: 1410 88 414 248 35 2490
Fragments arranged by size:
2490 1410 414 248 88 35
BanII G_rGCy'C
Cuts at: 193 811 1010 1487 1603 1699 2729 193
Size: 618 199 477 116 96 1030 2149
Fragments arranged by size:
2149 1030 618 477 199 116 96
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4437
BbsI GAAGACnn'nnnn_
Cuts at: 223 388 223
Size: 165 4520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1632 1666 1632
Size: 34 4651
BciVI GTATCCnnnnn_n'
Cuts at: 2583 3344 4685 2583
Size: 761 1341 2583
Fragments arranged by size:
2583 1341 761
BclI T'GATC_A
Cuts at: 1461 2205 1461
Size: 744 3941
BglI GCCn_nnn'nGGC
Cuts at: 140 967 140
Size: 827 3858
BglII A'GATC_T
Cuts at: 896 2200 4237 896
Size: 1304 2037 1344
Fragments arranged by size:
2037 1344 1304
BlpI GC'TnA_GC
Cuts at: 1797 1797
Size: 4685
Bme1580I G_kGCm'C
Cuts at: 811 1603 2310 2403 3459 811
Size: 792 707 93 1056 2037
Fragments arranged by size:
2037 1056 792 707 93
BmrI ACTGGGnnnn_n'
Cuts at: 1632 1897 1978 2313 4457 1632
Size: 265 81 335 2144 1860
Fragments arranged by size:
2144 1860 335 265 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 519 3052 519
Size: 2533 2152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 589 1246 1396 514
Size: 75 657 150 3803
Fragments arranged by size:
3803 657 150 75
Bpu10I CC'TnA_GC
Cuts at: 1241 1241
Size: 4685
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1435 1861 2740 3232 3530 1435
Size: 426 879 492 298 2590
Fragments arranged by size:
2590 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 4389 4389
Size: 4685
BsaAI yAC'GTr
Cuts at: 1196 1305 2038 1196
Size: 109 733 3843
Fragments arranged by size:
3843 733 109
BsaBI GATnn'nnATC
Cuts at: 6 1498 2199 4236 6
Size: 1492 701 2037 455
Fragments arranged by size:
2037 1492 701 455
BsaHI Gr'CG_yC
Cuts at: 223 2116 2364 3066 4562 223
Size: 1893 248 702 1496 346
Fragments arranged by size:
1893 1496 702 346 248
BsaWI w'CCGG_w
Cuts at: 1874 2395 3073 3347 3494 3905 1874
Size: 521 678 274 147 411 2654
Fragments arranged by size:
2654 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1189 1219 1189
Size: 30 4655
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 373 628 631 805 1243 370
Size: 3 255 3 174 438 3812
Fragments arranged by size:
3812 438 255 174 3 3
BseYI C'CCAG_C
Cuts at: 674 1029 2110 2868 3445 3941 674
Size: 355 1081 758 577 496 1418
Fragments arranged by size:
1418 1081 758 577 496 355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 1272 1274 1246
Size: 26 2 4657
Fragments arranged by size:
4657 26 2
BsiEI CG_ry'CG
Cuts at: 104 2227 2273 3481 104
Size: 2123 46 1208 1308
Fragments arranged by size:
2123 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 505 1147 1699 2477 2667 3459 505
Size: 642 552 778 190 792 1731
Fragments arranged by size:
1731 792 778 642 552 190
BsiWI C'GTAC_G
Cuts at: 1196 1196
Size: 4685
BsmI GAATG_Cn'
Cuts at: 184 710 184
Size: 526 4159
Bsp1286I G_dGCh'C
Cuts at: 193 505 811 1010 1147 1487 1603 1699 2310
Size: 312 306 199 137 340 116 96 611
Cuts at: 2310 2403 2477 2667 2729 3459 193
Size: 93 74 190 62 730 1419
Fragments arranged by size:
1419 730 611 340 312 306 199 190
137 116 96 93 74 62
BspEI T'CCGG_A
Cuts at: 3905 3905
Size: 4685
BspHI T'CATG_A
Cuts at: 3861 3861
Size: 4685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1735 1949 2251 2632 1735
Size: 214 302 381 3788
Fragments arranged by size:
3788 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2977 3031 4515 4680 50
Size: 2927 54 1484 165 55
Fragments arranged by size:
2927 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1650 1656 2597 1650
Size: 6 941 3738
Fragments arranged by size:
3738 941 6
BsrFI r'CCGG_y
Cuts at: 154 2683 2864 3073 154
Size: 2529 181 209 1766
Fragments arranged by size:
2529 1766 209 181
BssSI C'ACGA_G
Cuts at: 1021 2956 3314 1021
Size: 1935 358 2392
Fragments arranged by size:
2392 1935 358
BstAPI GCAn_nnn'nTGC
Cuts at: 252 2062 252
Size: 1810 2875
BstBI TT'CG_AA
Cuts at: 3046 3046
Size: 4685
BstEII G'GTnAC_C
Cuts at: 1080 1080
Size: 4685
BstXI CCAn_nnnn'nTGG
Cuts at: 138 1224 1610 138
Size: 1086 386 3213
Fragments arranged by size:
3213 1086 386
BstYI r'GATC_y
Cuts at: 1 362 581 896 1400 1499 1518 1710 2177
Size: 361 219 315 504 99 19 192 467
Cuts at: 2177 2200 2535 2781 3793 3879 3891 4237 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 504 467 449 361 346 335 315
246 219 192 99 86 23 19 12
Bsu36I CC'TnA_GG
Cuts at: 650 650
Size: 4685
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2635 2816 2979 2635
Size: 181 163 4341
Fragments arranged by size:
4341 181 163
BtsI GCAGTG_nn'
Cuts at: 203 245 246 1462 1928 203
Size: 42 1 1216 466 2960
Fragments arranged by size:
2960 1216 466 42 1
DraI TTT'AAA
Cuts at: 1684 1684
Size: 4685
DrdI GACnn_nn'nnGTC
Cuts at: 2391 3249 2391
Size: 858 3827
EaeI y'GGCC_r
Cuts at: 128 623 818 2270 2444 2835 2862 4207 128
Size: 495 195 1452 174 391 27 1345 606
Fragments arranged by size:
1452 1345 606 495 391 195 174 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4685
EarI CTCTTCn'nnn_
Cuts at: 124 1166 2708 2918 124
Size: 1042 1542 210 1891
Fragments arranged by size:
1891 1542 1042 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3203 3349 4495 2893
Size: 310 146 1146 3083
Fragments arranged by size:
3083 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1697 1697
Size: 4685
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 514 589 1246 1396 2510 2942 3689 3957
Size: 156 75 657 150 1114 432 747 268
Cuts at: 3957 358
Size: 1086
Fragments arranged by size:
1114 1086 747 657 432 268 156 150
75
EcoNI CCTnn'n_nnAGG
Cuts at: 1072 1225 1507 1072
Size: 153 282 4250
Fragments arranged by size:
4250 282 153
EcoO109I rG'GnC_Cy
Cuts at: 808 1026 1599 1824 808
Size: 218 573 225 3669
Fragments arranged by size:
3669 573 225 218
EcoRI G'AATT_C
Cuts at: 1063 1689 1063
Size: 626 4059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4685
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4685
HaeII r_GCGC'y
Cuts at: 2119 2367 3389 4368 2119
Size: 248 1022 979 2436
Fragments arranged by size:
2436 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2527 2557 2559 2589 2527
Size: 30 2 30 4623
Fragments arranged by size:
4623 30 30 2
HincII GTy'rAC
Cuts at: 1724 1724
Size: 4685
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4437
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4685
MluI A'CGCG_T
Cuts at: 4118 4118
Size: 4685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 251 1148 3356 3540 251
Size: 897 2208 184 1396
Fragments arranged by size:
2208 1396 897 184
MscI TGG'CCA
Cuts at: 130 625 2446 130
Size: 495 1821 2369
Fragments arranged by size:
2369 1821 495
MslI CAynn'nnrTG
Cuts at: 715 2674 2801 715
Size: 1959 127 2599
Fragments arranged by size:
2599 1959 127
MspA1I CmG'CkG
Cuts at: 325 567 1796 2110 2470 3483 3728 325
Size: 242 1229 314 360 1013 245 1282
Fragments arranged by size:
1282 1229 1013 360 314 245 242
NaeI GCC'GGC
Cuts at: 156 2866 156
Size: 2710 1975
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4437
NdeI CA'TA_TG
Cuts at: 301 826 301
Size: 525 4160
NgoMIV G'CCGG_C
Cuts at: 154 2864 154
Size: 2710 1975
NspI r_CATG'y
Cuts at: 414 784 885 1260 1738 2238 3145 414
Size: 370 101 375 478 500 907 1954
Fragments arranged by size:
1954 907 500 478 375 370 101
PciI A'CATG_T
Cuts at: 410 780 3141 410
Size: 370 2361 1954
Fragments arranged by size:
2361 1954 370
PflMI CCAn_nnn'nTGG
Cuts at: 771 1185 771
Size: 414 4271
PfoI T'CCnGG_A
Cuts at: 458 458
Size: 4685
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4685
PmlI CAC'GTG
Cuts at: 1305 1305
Size: 4685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 3824
Size: 32 4653
PpuMI rG'GwC_Cy
Cuts at: 1026 1026
Size: 4685
PshAI GACnn'nnGTC
Cuts at: 952 952
Size: 4685
PspOMI G'GGCC_C
Cuts at: 807 1599 807
Size: 792 3893
PstI C_TGCA'G
Cuts at: 257 1429 1732 2417 257
Size: 1172 303 685 2525
Fragments arranged by size:
2525 1172 685 303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4685
PvuII CAG'CTG
Cuts at: 325 567 2110 2470 325
Size: 242 1543 360 2540
Fragments arranged by size:
2540 1543 360 242
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4685
SacI G_AGCT'C
Cuts at: 1699 1699
Size: 4685
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4685
SapI GCTCTTCn'nnn_
Cuts at: 2708 2918 2708
Size: 210 4475
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4685
ScaI AGT'ACT
Cuts at: 4202 4202
Size: 4685
SfcI C'TryA_G
Cuts at: 33 253 1425 1728 2413 3406 3597 33
Size: 220 1172 303 685 993 191 1121
Fragments arranged by size:
1172 1121 993 685 303 220 191
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4437
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4685
SmaI CCC'GGG
Cuts at: 1707 1707
Size: 4685
SmlI C'TyrA_G
Cuts at: 1414 1840 2755 3247 3509 1414
Size: 426 915 492 262 2590
Fragments arranged by size:
2590 915 492 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4685
SphI G_CATG'C
Cuts at: 1260 1738 2238 1260
Size: 478 500 3707
Fragments arranged by size:
3707 500 478
StuI AGG'CCT
Cuts at: 769 769
Size: 4685
StyI C'CwwG_G
Cuts at: 165 770 890 1819 2173 165
Size: 605 120 929 354 2677
Fragments arranged by size:
2677 929 605 354 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2268 2401 2860 3069 2268
Size: 133 459 209 3884
Fragments arranged by size:
3884 459 209 133
TatI w'GTAC_w
Cuts at: 4200 4200
Size: 4685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4202 4234 4202
Size: 32 4653
Tth111I GACn'n_nGTC
Cuts at: 222 313 2482 222
Size: 91 2169 2425
Fragments arranged by size:
2425 2169 91
XbaI T'CTAG_A
Cuts at: 1716 1716
Size: 4685
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 948 2180 948
Size: 1232 3453
XmaI C'CCGG_G
Cuts at: 1705 1705
Size: 4685
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsmBI BsrGI
BssHII BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI