Restriction map for
pF1KE2763
(Circular) (Six-base) MAPSORT of: pF1KE2763.seq Check: 2447 from: 1 to: 3467
pF1KE2763 3467 bp
With 182 enzymes: SgfI *
October 3, 2019 17:18 ..
AccI GT'mk_AC
Cuts at: 505 505
Size: 3467
Acc65I G'GTAC_C
Cuts at: 483 483
Size: 3467
AclI AA'CG_TT
Cuts at: 3307 3307
Size: 3467
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 472 1292 1724 2471 2739 361
Size: 111 820 432 747 268 1089
Fragments arranged by size:
1089 820 747 432 268 111
AflIII A'CryG_T
Cuts at: 819 1923 2900 819
Size: 1104 977 1386
Fragments arranged by size:
1386 1104 977
AgeI A'CCGG_T
Cuts at: 1855 1855
Size: 3467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 383 383
Size: 3467
AlwNI CAG_nnn'CTG
Cuts at: 772 2339 772
Size: 1567 1900
ApaLI G'TGCA_C
Cuts at: 2237 2237
Size: 3467
ApoI r'AATT_y
Cuts at: 471 2820 3076 471
Size: 2349 256 862
Fragments arranged by size:
2349 862 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
AvaI C'yCGr_G
Cuts at: 487 487
Size: 3467
BamHI G'GATC_C
Cuts at: 492 492
Size: 3467
BanI G'GyrC_C
Cuts at: 132 483 897 1145 1180 132
Size: 351 414 248 35 2419
Fragments arranged by size:
2419 414 351 248 35
BanII G_rGCy'C
Cuts at: 325 481 1511 325
Size: 156 1030 2281
Fragments arranged by size:
2281 1030 156
BbeI G_GCGC'C
Cuts at: 901 1149 901
Size: 248 3219
BbsI GAAGACnn'nnnn_
Cuts at: 150 186 460 150
Size: 36 274 3157
Fragments arranged by size:
3157 274 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3433
BciVI GTATCCnnnnn_n'
Cuts at: 1365 2126 3467 1365
Size: 761 1341 1365
Fragments arranged by size:
1365 1341 761
BclI T'GATC_A
Cuts at: 987 987
Size: 3467
BglI GCCn_nnn'nGGC
Cuts at: 263 263
Size: 3467
BglII A'GATC_T
Cuts at: 982 3019 982
Size: 2037 1430
BlpI GC'TnA_GC
Cuts at: 579 579
Size: 3467
Bme1580I G_kGCm'C
Cuts at: 241 1092 1185 2241 241
Size: 851 93 1056 1467
Fragments arranged by size:
1467 1056 851 93
BmgBI CAC'GTC
Cuts at: 354 354
Size: 3467
BmrI ACTGGGnnnn_n'
Cuts at: 679 760 1095 3239 679
Size: 81 335 2144 907
Fragments arranged by size:
2144 907 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1834 1834
Size: 3467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 419
Size: 3467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 643 1522 2014 2312 643
Size: 879 492 298 1798
Fragments arranged by size:
1798 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3171 3171
Size: 3467
BsaAI yAC'GTr
Cuts at: 820 820
Size: 3467
BsaBI GATnn'nnATC
Cuts at: 6 981 3018 6
Size: 975 2037 455
Fragments arranged by size:
2037 975 455
BsaHI Gr'CG_yC
Cuts at: 191 898 1146 1848 3344 191
Size: 707 248 702 1496 314
Fragments arranged by size:
1496 707 702 314 248
BsaWI w'CCGG_w
Cuts at: 656 1177 1855 2129 2276 2687 656
Size: 521 678 274 147 411 1436
Fragments arranged by size:
1436 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 307 337 417 447 307
Size: 30 80 30 3327
Fragments arranged by size:
3327 80 30 30
BseYI C'CCAG_C
Cuts at: 892 1650 2227 2723 892
Size: 758 577 496 1636
Fragments arranged by size:
1636 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 283
Size: 3467
BsiEI CG_ry'CG
Cuts at: 104 1009 1055 2263 104
Size: 905 46 1208 1308
Fragments arranged by size:
1308 1208 905 46
BsiHKAI G_wGCw'C
Cuts at: 481 1259 1449 2241 481
Size: 778 190 792 1707
Fragments arranged by size:
1707 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 241 325 481 1092 1185 1259 1449 1511 2241
Size: 84 156 611 93 74 190 62 730
Cuts at: 2241 241
Size: 1467
Fragments arranged by size:
1467 730 611 190 156 93 84 74
62
BspEI T'CCGG_A
Cuts at: 2687 2687
Size: 3467
BspHI T'CATG_A
Cuts at: 2643 2643
Size: 3467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 305 450 517 731 1033 1414 305
Size: 145 67 214 302 381 2358
Fragments arranged by size:
2358 381 302 214 145 67
BsrBI CCG'CTC
Cuts at: 50 1759 1813 3297 3462 50
Size: 1709 54 1484 165 55
Fragments arranged by size:
1709 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1379 1379
Size: 3467
BsrFI r'CCGG_y
Cuts at: 1465 1646 1855 1465
Size: 181 209 3077
Fragments arranged by size:
3077 209 181
BsrGI T'GTAC_A
Cuts at: 332 332
Size: 3467
BssSI C'ACGA_G
Cuts at: 1738 2096 1738
Size: 358 3109
BstAPI GCAn_nnn'nTGC
Cuts at: 844 844
Size: 3467
BstBI TT'CG_AA
Cuts at: 1828 1828
Size: 3467
BstYI r'GATC_y
Cuts at: 1 492 959 982 1317 1563 2575 2661 2673
Size: 491 467 23 335 246 1012 86 12
Cuts at: 2673 3019 1
Size: 346 449
Fragments arranged by size:
1012 491 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 224 224
Size: 3467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1417 1598 1761 1417
Size: 181 163 3123
Fragments arranged by size:
3123 181 163
BtsI GCAGTG_nn'
Cuts at: 710 710
Size: 3467
DraI TTT'AAA
Cuts at: 466 466
Size: 3467
DrdI GACnn_nn'nnGTC
Cuts at: 1173 2031 1173
Size: 858 2609
EaeI y'GGCC_r
Cuts at: 1052 1226 1617 1644 2989 1052
Size: 174 391 27 1345 1530
Fragments arranged by size:
1530 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1052 1052
Size: 3467
EarI CTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1675 1985 2131 3277 1675
Size: 310 146 1146 1865
Fragments arranged by size:
1865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 479 479
Size: 3467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 419 472 1292 1724 2471 2739 361
Size: 58 53 820 432 747 268 1089
Fragments arranged by size:
1089 820 747 432 268 58 53
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 3467
EcoO109I rG'GnC_Cy
Cuts at: 255 606 255
Size: 351 3116
EcoRI G'AATT_C
Cuts at: 471 471
Size: 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 381 1486 381
Size: 1105 2362
FspI TGC'GCA
Cuts at: 1248 1248
Size: 3467
HaeII r_GCGC'y
Cuts at: 901 1149 2171 3150 901
Size: 248 1022 979 1218
Fragments arranged by size:
1218 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 409 1309 1339 1341 1371 377
Size: 32 900 30 2 30 2473
Fragments arranged by size:
2473 900 32 30 30 2
HincII GTy'rAC
Cuts at: 506 506
Size: 3467
KasI G'GCGC_C
Cuts at: 897 1145 897
Size: 248 3219
KpnI G_GTAC'C
Cuts at: 487 487
Size: 3467
MluI A'CGCG_T
Cuts at: 2900 2900
Size: 3467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2322 2138
Size: 184 3283
MscI TGG'CCA
Cuts at: 1228 1228
Size: 3467
MslI CAynn'nnrTG
Cuts at: 1456 1583 1456
Size: 127 3340
MspA1I CmG'CkG
Cuts at: 578 892 1252 2265 2510 578
Size: 314 360 1013 245 1535
Fragments arranged by size:
1535 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1648 1648
Size: 3467
NarI GG'CG_CC
Cuts at: 898 1146 898
Size: 248 3219
NgoMIV G'CCGG_C
Cuts at: 1646 1646
Size: 3467
NspI r_CATG'y
Cuts at: 210 520 1020 1927 210
Size: 310 500 907 1750
Fragments arranged by size:
1750 907 500 310
PciI A'CATG_T
Cuts at: 1923 1923
Size: 3467
PmeI GTTT'AAAC
Cuts at: 466 466
Size: 3467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2606 2638 2606
Size: 32 3435
PstI C_TGCA'G
Cuts at: 131 514 1199 131
Size: 383 685 2399
Fragments arranged by size:
2399 685 383
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3467
PvuII CAG'CTG
Cuts at: 892 1252 892
Size: 360 3107
RsrII CG'GwC_CG
Cuts at: 1662 1662
Size: 3467
SacI G_AGCT'C
Cuts at: 481 481
Size: 3467
SalI G'TCGA_C
Cuts at: 504 504
Size: 3467
SapI GCTCTTCn'nnn_
Cuts at: 1490 1700 1490
Size: 210 3257
SbfI CC_TGCA'GG
Cuts at: 514 514
Size: 3467
ScaI AGT'ACT
Cuts at: 2984 2984
Size: 3467
SfcI C'TryA_G
Cuts at: 33 127 510 1195 2188 2379 33
Size: 94 383 685 993 191 1121
Fragments arranged by size:
1121 993 685 383 191 94
SfoI GGC'GCC
Cuts at: 899 1147 899
Size: 248 3219
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3467
SmaI CCC'GGG
Cuts at: 489 489
Size: 3467
SmlI C'TyrA_G
Cuts at: 622 1537 2029 2291 622
Size: 915 492 262 1798
Fragments arranged by size:
1798 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3467
SphI G_CATG'C
Cuts at: 210 520 1020 210
Size: 310 500 2657
Fragments arranged by size:
2657 500 310
StyI C'CwwG_G
Cuts at: 601 955 601
Size: 354 3113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1050 1183 1642 1851 1050
Size: 133 459 209 2666
Fragments arranged by size:
2666 459 209 133
TatI w'GTAC_w
Cuts at: 332 2982 332
Size: 2650 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 307 339 417 449 2984 3016 307
Size: 32 78 32 2535 32 758
Fragments arranged by size:
2535 758 78 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1264 1264
Size: 3467
XbaI T'CTAG_A
Cuts at: 498 498
Size: 3467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 962 962
Size: 3467
XmaI C'CCGG_G
Cuts at: 487 487
Size: 3467
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI