Restriction map for
pF1KE2758
(Circular) (Six-base) MAPSORT of: pF1KE2758.seq Check: 521 from: 1 to: 5075
pF1KE2758 5075 bp
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AccI GT'mk_AC
Cuts at: 2113 2113
Size: 5075
Acc65I G'GTAC_C
Cuts at: 2091 2091
Size: 5075
AclI AA'CG_TT
Cuts at: 4915 4915
Size: 5075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 437 2900 3332 4079 4347 136
Size: 301 2463 432 747 268 864
Fragments arranged by size:
2463 864 747 432 301 268
AflIII A'CryG_T
Cuts at: 2427 3531 4508 2427
Size: 1104 977 2994
Fragments arranged by size:
2994 1104 977
AgeI A'CCGG_T
Cuts at: 3463 3463
Size: 5075
AlwNI CAG_nnn'CTG
Cuts at: 2380 3947 2380
Size: 1567 3508
ApaI G_GGCC'C
Cuts at: 324 550 1859 324
Size: 226 1309 3540
Fragments arranged by size:
3540 1309 226
ApaLI G'TGCA_C
Cuts at: 3845 3845
Size: 5075
ApoI r'AATT_y
Cuts at: 863 2079 4428 4684 863
Size: 1216 2349 256 1254
Fragments arranged by size:
2349 1254 1216 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5075
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
AvaI C'yCGr_G
Cuts at: 895 2095 895
Size: 1200 3875
AvrII C'CTAG_G
Cuts at: 595 595
Size: 5075
BamHI G'GATC_C
Cuts at: 2100 2100
Size: 5075
BanI G'GyrC_C
Cuts at: 2091 2505 2753 2788 2091
Size: 414 248 35 4378
Fragments arranged by size:
4378 414 248 35
BanII G_rGCy'C
Cuts at: 152 324 550 896 1248 1338 1859 2089 3119
Size: 172 226 346 352 90 521 230 1030
Cuts at: 3119 152
Size: 2108
Fragments arranged by size:
2108 1030 521 352 346 230 226 172
90
BbeI G_GCGC'C
Cuts at: 2509 2757 2509
Size: 248 4827
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5041
BciVI GTATCCnnnnn_n'
Cuts at: 2973 3734 5075 2973
Size: 761 1341 2973
Fragments arranged by size:
2973 1341 761
BclI T'GATC_A
Cuts at: 585 639 2595 585
Size: 54 1956 3065
Fragments arranged by size:
3065 1956 54
BglI GCCn_nnn'nGGC
Cuts at: 217 319 473 716 1700 1727 1863 1997 217
Size: 102 154 243 984 27 136 134 3295
Fragments arranged by size:
3295 984 243 154 136 134 102 27
BglII A'GATC_T
Cuts at: 2590 4627 2590
Size: 2037 3038
BlpI GC'TnA_GC
Cuts at: 2187 2187
Size: 5075
Bme1580I G_kGCm'C
Cuts at: 192 324 550 1859 2700 2793 3849 192
Size: 132 226 1309 841 93 1056 1418
Fragments arranged by size:
1418 1309 1056 841 226 132 93
BmrI ACTGGGnnnn_n'
Cuts at: 2287 2368 2703 4847 2287
Size: 81 335 2144 2515
Fragments arranged by size:
2515 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5075
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 464 680 698 926 971 998 1262 1352
Size: 196 216 18 228 45 27 264 90
Cuts at: 1352 1496 268
Size: 144 3847
Fragments arranged by size:
3847 264 228 216 196 144 90 45
27 18
Bpu10I CC'TnA_GC
Cuts at: 1337 1337
Size: 5075
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 1136 1460 2251 3130 3622 3920 346
Size: 790 324 791 879 492 298 1501
Fragments arranged by size:
1501 879 791 790 492 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 4779 4779
Size: 5075
BsaAI yAC'GTr
Cuts at: 2428 2428
Size: 5075
BsaBI GATnn'nnATC
Cuts at: 6 2589 4626 6
Size: 2583 2037 455
Fragments arranged by size:
2583 2037 455
BsaHI Gr'CG_yC
Cuts at: 2506 2754 3456 4952 2506
Size: 248 702 1496 2629
Fragments arranged by size:
2629 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2264 2785 3463 3737 3884 4295 2264
Size: 521 678 274 147 411 3044
Fragments arranged by size:
3044 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 524 524
Size: 5075
BseYI C'CCAG_C
Cuts at: 468 2500 3258 3835 4331 468
Size: 2032 758 577 496 1212
Fragments arranged by size:
2032 1212 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2617 2663 3871 104
Size: 2513 46 1208 1308
Fragments arranged by size:
2513 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 858 1338 2089 2867 3057 3849 858
Size: 480 751 778 190 792 2084
Fragments arranged by size:
2084 792 778 751 480 190
BsmI GAATG_Cn'
Cuts at: 770 1667 770
Size: 897 4178
BsmBI CGTCTCn'nnnn_
Cuts at: 965 965
Size: 5075
Bsp1286I G_dGCh'C
Cuts at: 152 192 324 550 858 896 1248 1338 1859
Size: 40 132 226 308 38 352 90 521
Cuts at: 1859 2089 2700 2793 2867 3057 3119 3849 152
Size: 230 611 93 74 190 62 730 1378
Fragments arranged by size:
1378 730 611 521 352 308 230 226
190 132 93 90 74 62 40 38
BspEI T'CCGG_A
Cuts at: 4295 4295
Size: 5075
BspHI T'CATG_A
Cuts at: 4251 4251
Size: 5075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1789 2125 2339 2641 3022 1789
Size: 336 214 302 381 3842
Fragments arranged by size:
3842 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 301 3367 3421 4905 5070 50
Size: 251 3066 54 1484 165 55
Fragments arranged by size:
3066 1484 251 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2987 2987
Size: 5075
BsrFI r'CCGG_y
Cuts at: 3073 3254 3463 3073
Size: 181 209 4685
Fragments arranged by size:
4685 209 181
BssSI C'ACGA_G
Cuts at: 3346 3704 3346
Size: 358 4717
BstAPI GCAn_nnn'nTGC
Cuts at: 2452 2452
Size: 5075
BstBI TT'CG_AA
Cuts at: 3436 3436
Size: 5075
BstEII G'GTnAC_C
Cuts at: 673 1015 673
Size: 342 4733
BstYI r'GATC_y
Cuts at: 1 154 1507 2100 2567 2590 2925 3171 4183
Size: 153 1353 593 467 23 335 246 1012
Cuts at: 4183 4269 4281 4627 1
Size: 86 12 346 449
Fragments arranged by size:
1353 1012 593 467 449 346 335 246
153 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 542 938 542
Size: 396 4679
BtgI C'CryG_G
Cuts at: 242 759 1716 1733 1788 1912 1959 242
Size: 517 957 17 55 124 47 3358
Fragments arranged by size:
3358 957 517 124 55 47 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3025 3206 3369 3025
Size: 181 163 4731
Fragments arranged by size:
4731 181 163
BtsI GCAGTG_nn'
Cuts at: 2318 2318
Size: 5075
DraI TTT'AAA
Cuts at: 2074 2074
Size: 5075
DraIII CAC_nnn'GTG
Cuts at: 1918 1918
Size: 5075
DrdI GACnn_nn'nnGTC
Cuts at: 536 1349 2781 3639 536
Size: 813 1432 858 1972
Fragments arranged by size:
1972 1432 858 813
EaeI y'GGCC_r
Cuts at: 239 2660 2834 3225 3252 4597 239
Size: 2421 174 391 27 1345 717
Fragments arranged by size:
2421 1345 717 391 174 27
EagI C'GGCC_G
Cuts at: 2660 2660
Size: 5075
EarI CTCTTCn'nnn_
Cuts at: 501 1215 3098 3308 501
Size: 714 1883 210 2268
Fragments arranged by size:
2268 1883 714 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 300 3283 3593 3739 4885 300
Size: 2983 310 146 1146 490
Fragments arranged by size:
2983 1146 490 310 146
EcoICRI GAG'CTC
Cuts at: 1336 2087 1336
Size: 751 4324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 268 437 464 680 698 926 971 998
Size: 132 169 27 216 18 228 45 27
Cuts at: 998 1262 1352 1496 2900 3332 4079 4347 136
Size: 264 90 144 1404 432 747 268 864
Fragments arranged by size:
1404 864 747 432 268 264 228 216
169 144 132 90 45 27 27 18
EcoNI CCTnn'n_nnAGG
Cuts at: 667 1220 1425 667
Size: 553 205 4317
Fragments arranged by size:
4317 553 205
EcoO109I rG'GnC_Cy
Cuts at: 162 320 321 475 546 547 592 691 700
Size: 158 1 154 71 1 45 99 9
Cuts at: 700 817 826 934 1210 1453 1572 1678 1711
Size: 117 9 108 276 243 119 106 33
Cuts at: 1711 1738 1783 1801 1855 1856 2214 162
Size: 27 45 18 54 1 358 3023
Fragments arranged by size:
3023 358 276 243 158 154 119 117
108 106 99 71 54 45 45 33
27 18 9 9 1 1 1
EcoRI G'AATT_C
Cuts at: 863 2079 863
Size: 1216 3859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 5075
FspI TGC'GCA
Cuts at: 2856 2856
Size: 5075
HaeII r_GCGC'y
Cuts at: 2509 2757 3779 4758 2509
Size: 248 1022 979 2826
Fragments arranged by size:
2826 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 285 317 804 836 1009 1041 1492
Size: 32 114 32 487 32 173 32 451
Cuts at: 1492 1524 2917 2947 2949 2979 139
Size: 32 1393 30 2 30 2235
Fragments arranged by size:
2235 1393 487 451 173 114 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2114 2114
Size: 5075
KasI G'GCGC_C
Cuts at: 2505 2753 2505
Size: 248 4827
KpnI G_GTAC'C
Cuts at: 2095 2095
Size: 5075
MfeI C'AATT_G
Cuts at: 2026 2026
Size: 5075
MluI A'CGCG_T
Cuts at: 4508 4508
Size: 5075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3746 3930 3746
Size: 184 4891
MscI TGG'CCA
Cuts at: 2836 2836
Size: 5075
MslI CAynn'nnrTG
Cuts at: 3064 3191 3064
Size: 127 4948
MspA1I CmG'CkG
Cuts at: 2186 2500 2860 3873 4118 2186
Size: 314 360 1013 245 3143
Fragments arranged by size:
3143 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3256 3256
Size: 5075
NarI GG'CG_CC
Cuts at: 2506 2754 2506
Size: 248 4827
NcoI C'CATG_G
Cuts at: 1716 1733 1788 1716
Size: 17 55 5003
Fragments arranged by size:
5003 55 17
NdeI CA'TA_TG
Cuts at: 419 419
Size: 5075
NgoMIV G'CCGG_C
Cuts at: 3254 3254
Size: 5075
NspI r_CATG'y
Cuts at: 2128 2628 3535 2128
Size: 500 907 3668
Fragments arranged by size:
3668 907 500
PciI A'CATG_T
Cuts at: 3531 3531
Size: 5075
PfoI T'CCnGG_A
Cuts at: 525 696 831 1107 1215 1467 1512 1611 1752
Size: 171 135 276 108 252 45 99 141
Cuts at: 1752 525
Size: 3848
Fragments arranged by size:
3848 276 252 171 141 135 108 99
45
PmeI GTTT'AAAC
Cuts at: 2074 2074
Size: 5075
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 4214
Size: 32 5043
PpuMI rG'GwC_Cy
Cuts at: 162 592 700 817 934 1453 1572 1678 1711
Size: 430 108 117 117 519 119 106 33
Cuts at: 1711 1738 1783 1801 162
Size: 27 45 18 3436
Fragments arranged by size:
3436 519 430 119 117 117 108 106
45 33 27 18
PspOMI G'GGCC_C
Cuts at: 320 546 1855 320
Size: 226 1309 3540
Fragments arranged by size:
3540 1309 226
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1151 1183 1151
Size: 32 5043
PstI C_TGCA'G
Cuts at: 1038 1800 2122 2807 1038
Size: 762 322 685 3306
Fragments arranged by size:
3306 762 685 322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5075
PvuII CAG'CTG
Cuts at: 2500 2860 2500
Size: 360 4715
RsrII CG'GwC_CG
Cuts at: 3270 3270
Size: 5075
SacI G_AGCT'C
Cuts at: 1338 2089 1338
Size: 751 4324
SalI G'TCGA_C
Cuts at: 2112 2112
Size: 5075
SanDI GG'GwC_CC
Cuts at: 1453 1572 1783 1453
Size: 119 211 4745
Fragments arranged by size:
4745 211 119
SapI GCTCTTCn'nnn_
Cuts at: 3098 3308 3098
Size: 210 4865
SbfI CC_TGCA'GG
Cuts at: 1800 2122 1800
Size: 322 4753
ScaI AGT'ACT
Cuts at: 4592 4592
Size: 5075
SexAI A'CCwGG_T
Cuts at: 1134 1170 1356 1707 1134
Size: 36 186 351 4502
Fragments arranged by size:
4502 351 186 36
SfcI C'TryA_G
Cuts at: 33 1034 1796 2118 2803 3796 3987 33
Size: 1001 762 322 685 993 191 1121
Fragments arranged by size:
1121 1001 993 762 685 322 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 716 1700 1727 1997 716
Size: 984 27 270 3794
Fragments arranged by size:
3794 984 270 27
SfoI GGC'GCC
Cuts at: 2507 2755 2507
Size: 248 4827
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5075
SmaI CCC'GGG
Cuts at: 897 2097 897
Size: 1200 3875
SmlI C'TyrA_G
Cuts at: 361 1151 1475 2230 3145 3637 3899 361
Size: 790 324 755 915 492 262 1537
Fragments arranged by size:
1537 915 790 755 492 324 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5075
SphI G_CATG'C
Cuts at: 2128 2628 2128
Size: 500 4575
StyI C'CwwG_G
Cuts at: 595 678 992 1345 1716 1733 1788 2209 2563
Size: 83 314 353 371 17 55 421 354
Cuts at: 2563 595
Size: 3107
Fragments arranged by size:
3107 421 371 354 353 314 83 55
17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 2658 2791 3250 3459 253
Size: 2405 133 459 209 1869
Fragments arranged by size:
2405 1869 459 209 133
TatI w'GTAC_w
Cuts at: 4590 4590
Size: 5075
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1254 1286 4592 4624 1254
Size: 32 3306 32 1705
Fragments arranged by size:
3306 1705 32 32
Tth111I GACn'n_nGTC
Cuts at: 2872 2872
Size: 5075
XbaI T'CTAG_A
Cuts at: 2106 2106
Size: 5075
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1723 2570 1723
Size: 847 4228
XmaI C'CCGG_G
Cuts at: 895 2095 895
Size: 1200 3875
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspXI
SacII SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI