Restriction map for
pF1KE2757
(Circular) (Six-base) MAPSORT of: pF1KE2757.seq Check: 8253 from: 1 to: 8396
pF1KE2757 8396 bp
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AccI GT'mk_AC
Cuts at: 5289 5434 5289
Size: 145 8251
Acc65I G'GTAC_C
Cuts at: 3508 5412 3508
Size: 1904 6492
AclI AA'CG_TT
Cuts at: 540 3432 8236 540
Size: 2892 4804 700
Fragments arranged by size:
4804 2892 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2801 3958 4547 6221 6653 7400 7668 2801
Size: 1157 589 1674 432 747 268 3529
Fragments arranged by size:
3529 1674 1157 747 589 432 268
AflIII A'CryG_T
Cuts at: 1277 1655 1695 2214 3776 5748 6852 7829 1277
Size: 378 40 519 1562 1972 1104 977 1844
Fragments arranged by size:
1972 1844 1562 1104 977 519 378 40
AgeI A'CCGG_T
Cuts at: 6784 6784
Size: 8396
AleI CACnn'nnGTG
Cuts at: 2309 2309
Size: 8396
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1847 1879 1847
Size: 32 8364
AlwNI CAG_nnn'CTG
Cuts at: 1127 1729 1867 2777 2898 5701 7268 1127
Size: 602 138 910 121 2803 1567 2255
Fragments arranged by size:
2803 2255 1567 910 602 138 121
ApaLI G'TGCA_C
Cuts at: 7166 7166
Size: 8396
ApoI r'AATT_y
Cuts at: 1752 2265 2990 3610 3826 3907 5101 5400 7749
Size: 513 725 620 216 81 1194 299 2349
Cuts at: 7749 8005 1752
Size: 256 2143
Fragments arranged by size:
2349 2143 1194 725 620 513 299 256
216 81
AseI AT'TA_AT
Cuts at: 20 4082 4103 20
Size: 4062 21 4313
Fragments arranged by size:
4313 4062 21
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8396
AvaI C'yCGr_G
Cuts at: 2349 3593 5416 2349
Size: 1244 1823 5329
Fragments arranged by size:
5329 1823 1244
AvrII C'CTAG_G
Cuts at: 3358 3358
Size: 8396
BamHI G'GATC_C
Cuts at: 717 2440 5421 717
Size: 1723 2981 3692
Fragments arranged by size:
3692 2981 1723
BanI G'GyrC_C
Cuts at: 184 1800 2353 3508 5412 5826 6074 6109 184
Size: 1616 553 1155 1904 414 248 35 2471
Fragments arranged by size:
2471 1904 1616 1155 553 414 248 35
BanII G_rGCy'C
Cuts at: 270 1813 2434 2908 4755 5410 6440 270
Size: 1543 621 474 1847 655 1030 2226
Fragments arranged by size:
2226 1847 1543 1030 655 621 474
BbeI G_GCGC'C
Cuts at: 1804 5830 6078 1804
Size: 4026 248 4122
Fragments arranged by size:
4122 4026 248
BbsI GAAGACnn'nnnn_
Cuts at: 1193 2920 3616 3870 3974 4604 1193
Size: 1727 696 254 104 630 4985
Fragments arranged by size:
4985 1727 696 630 254 104
BbvCI CC'TCA_GC
Cuts at: 1168 2325 4486 1168
Size: 1157 2161 5078
Fragments arranged by size:
5078 2161 1157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2097 2131 4594 4605 4628 4639 90
Size: 34 1973 34 2463 11 23 11 3847
Fragments arranged by size:
3847 2463 1973 34 34 23 11 11
BciVI GTATCCnnnnn_n'
Cuts at: 1512 1576 3301 6294 7055 8396 1512
Size: 64 1725 2993 761 1341 1512
Fragments arranged by size:
2993 1725 1512 1341 761 64
BclI T'GATC_A
Cuts at: 441 2369 3645 5304 5916 441
Size: 1928 1276 1659 612 2921
Fragments arranged by size:
2921 1928 1659 1276 612
BfrBI ATG'CAT
Cuts at: 3482 3482
Size: 8396
BglI GCCn_nnn'nGGC
Cuts at: 2663 2663
Size: 8396
BglII A'GATC_T
Cuts at: 3915 5911 7948 3915
Size: 1996 2037 4363
Fragments arranged by size:
4363 2037 1996
BlpI GC'TnA_GC
Cuts at: 259 5508 259
Size: 5249 3147
Bme1580I G_kGCm'C
Cuts at: 2617 3594 4265 4307 6021 6114 7170 2617
Size: 977 671 42 1714 93 1056 3843
Fragments arranged by size:
3843 1714 1056 977 671 93 42
BmrI ACTGGGnnnn_n'
Cuts at: 716 1596 1986 4507 5608 5689 6024 8168 716
Size: 880 390 2521 1101 81 335 2144 944
Fragments arranged by size:
2521 2144 1101 944 880 390 335 81
BmtI G_CTAG'C
Cuts at: 923 923
Size: 8396
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 6763 3247
Size: 3516 4880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1063 1104 2057 4577 4981 5008 5240 316
Size: 747 41 953 2520 404 27 232 3472
Fragments arranged by size:
3472 2520 953 747 404 232 41 27
Bpu10I CC'TnA_GC
Cuts at: 1168 2325 4486 1168
Size: 1157 2161 5078
Fragments arranged by size:
5078 2161 1157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 1240 1947 2324 2555 2844 4838 5113 5572
Size: 1091 707 377 231 289 1994 275 459
Cuts at: 5572 6451 6943 7241 149
Size: 879 492 298 1304
Fragments arranged by size:
1994 1304 1091 879 707 492 459 377
298 289 275 231
BsaI GGTCTCn'nnnn_
Cuts at: 3724 8100 3724
Size: 4376 4020
BsaAI yAC'GTr
Cuts at: 1148 5749 1148
Size: 4601 3795
BsaBI GATnn'nnATC
Cuts at: 6 2294 5279 5910 7947 6
Size: 2288 2985 631 2037 455
Fragments arranged by size:
2985 2288 2037 631 455
BsaHI Gr'CG_yC
Cuts at: 1801 5827 6075 6777 8273 1801
Size: 4026 248 702 1496 1924
Fragments arranged by size:
4026 1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1552 5585 6106 6784 7058 7205 7616 1552
Size: 4033 521 678 274 147 411 2332
Fragments arranged by size:
4033 2332 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 635 665 686 716 1847 1877 635
Size: 30 21 30 1131 30 7154
Fragments arranged by size:
7154 1131 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 4017 4017
Size: 8396
BseYI C'CCAG_C
Cuts at: 2680 5083 5821 6579 7156 7652 2680
Size: 2403 738 758 577 496 3424
Fragments arranged by size:
3424 2403 758 738 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1122 4272 1122
Size: 3150 5246
BsiEI CG_ry'CG
Cuts at: 104 1224 5938 5984 7192 104
Size: 1120 4714 46 1208 1308
Fragments arranged by size:
4714 1308 1208 1120 46
BsiHKAI G_wGCw'C
Cuts at: 1560 1663 2051 2348 2515 2908 3003 4284 5410
Size: 103 388 297 167 393 95 1281 1126
Cuts at: 5410 6188 6378 7170 1560
Size: 778 190 792 2786
Fragments arranged by size:
2786 1281 1126 792 778 393 388 297
190 167 103 95
BsmI GAATG_Cn'
Cuts at: 1283 1576 1690 2965 3482 4362 1283
Size: 293 114 1275 517 880 5317
Fragments arranged by size:
5317 1275 880 517 293 114
BsmBI CGTCTCn'nnnn_
Cuts at: 1810 1810
Size: 8396
Bsp1286I G_dGCh'C
Cuts at: 270 1560 1663 1813 2051 2348 2434 2515 2617
Size: 1290 103 150 238 297 86 81 102
Cuts at: 2617 2908 3003 3594 4265 4284 4307 4755 5410
Size: 291 95 591 671 19 23 448 655
Cuts at: 5410 6021 6114 6188 6378 6440 7170 270
Size: 611 93 74 190 62 730 1496
Fragments arranged by size:
1496 1290 730 671 655 611 591 448
297 291 238 190 150 103 102 95
93 86 81 74 62 23 19
BspEI T'CCGG_A
Cuts at: 7616 7616
Size: 8396
BspHI T'CATG_A
Cuts at: 3849 7572 3849
Size: 3723 4673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3169 3496 5446 5660 5962 6343 3169
Size: 327 1950 214 302 381 5222
Fragments arranged by size:
5222 1950 381 327 302 214
BsrBI CCG'CTC
Cuts at: 50 1254 6688 6742 8226 8391 50
Size: 1204 5434 54 1484 165 55
Fragments arranged by size:
5434 1484 1204 165 55 54
BsrDI GCAATG_nn'
Cuts at: 533 915 1970 2336 4016 6308 533
Size: 382 1055 366 1680 2292 2621
Fragments arranged by size:
2621 2292 1680 1055 382 366
BsrFI r'CCGG_y
Cuts at: 1366 6394 6575 6784 1366
Size: 5028 181 209 2978
Fragments arranged by size:
5028 2978 209 181
BsrGI T'GTAC_A
Cuts at: 2151 2151
Size: 8396
BssSI C'ACGA_G
Cuts at: 6667 7025 6667
Size: 358 8038
BstAPI GCAn_nnn'nTGC
Cuts at: 945 1100 3170 3443 4018 5773 945
Size: 155 2070 273 575 1755 3568
Fragments arranged by size:
3568 2070 1755 575 273 155
BstBI TT'CG_AA
Cuts at: 6757 6757
Size: 8396
BstXI CCAn_nnnn'nTGG
Cuts at: 814 814
Size: 8396
BstYI r'GATC_y
Cuts at: 1 717 1269 2440 3915 4239 5421 5888 5911
Size: 716 552 1171 1475 324 1182 467 23
Cuts at: 5911 6246 6492 7504 7590 7602 7948 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1475 1182 1171 1012 716 552 467 449
346 335 324 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 784 1353 784
Size: 569 7827
BtgI C'CryG_G
Cuts at: 1608 2622 3200 1608
Size: 1014 578 6804
Fragments arranged by size:
6804 1014 578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3492 6346 6527 6690 3492
Size: 2854 181 163 5198
Fragments arranged by size:
5198 2854 181 163
BtsI GCAGTG_nn'
Cuts at: 1215 1560 1684 2049 2559 3444 3905 5639 1215
Size: 345 124 365 510 885 461 1734 3972
Fragments arranged by size:
3972 1734 885 510 461 365 345 124
DraI TTT'AAA
Cuts at: 707 5395 707
Size: 4688 3708
DrdI GACnn_nn'nnGTC
Cuts at: 2011 2070 2828 5041 6102 6960 2011
Size: 59 758 2213 1061 858 3447
Fragments arranged by size:
3447 2213 1061 858 758 59
EaeI y'GGCC_r
Cuts at: 624 912 1364 1368 1704 1824 2637 3570 5981
Size: 288 452 4 336 120 813 933 2411
Cuts at: 5981 6155 6546 6573 7918 624
Size: 174 391 27 1345 1102
Fragments arranged by size:
2411 1345 1102 933 813 452 391 336
288 174 120 27 4
EagI C'GGCC_G
Cuts at: 5981 5981
Size: 8396
EarI CTCTTCn'nnn_
Cuts at: 1243 4945 6419 6629 1243
Size: 3702 1474 210 3010
Fragments arranged by size:
3702 3010 1474 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6604 6914 7060 8206 6604
Size: 310 146 1146 6794
Fragments arranged by size:
6794 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2906 5408 2906
Size: 2502 5894
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1063 1104 2057 2801 3958 4547 4577 4981
Size: 747 41 953 744 1157 589 30 404
Cuts at: 4981 5008 5240 6221 6653 7400 7668 316
Size: 27 232 981 432 747 268 1044
Fragments arranged by size:
1157 1044 981 953 747 747 744 589
432 404 268 232 41 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1352 2189 2361 2732 2816 3329 1352
Size: 837 172 371 84 513 6419
Fragments arranged by size:
6419 837 513 371 172 84
EcoO109I rG'GnC_Cy
Cuts at: 1248 1590 2335 3362 4321 5535 1248
Size: 342 745 1027 959 1214 4109
Fragments arranged by size:
4109 1214 1027 959 745 342
EcoRI G'AATT_C
Cuts at: 3610 5400 3610
Size: 1790 6606
EcoRV GAT'ATC
Cuts at: 4209 4209
Size: 8396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1059 2301 6415 1059
Size: 1242 4114 3040
Fragments arranged by size:
4114 3040 1242
FseI GG_CCGG'CC
Cuts at: 1370 1370
Size: 8396
FspI TGC'GCA
Cuts at: 6177 6177
Size: 8396
HaeII r_GCGC'y
Cuts at: 1445 1804 2499 5830 6078 7100 8079 1445
Size: 359 695 3331 248 1022 979 1762
Fragments arranged by size:
3331 1762 1022 979 695 359 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1922 1954 2497 2529 2722 2754 2842 2874 3037
Size: 32 543 32 193 32 88 32 163
Cuts at: 3037 3069 3134 3166 3567 3599 3979 4011 6238
Size: 32 65 32 401 32 380 32 2227
Cuts at: 6238 6268 6270 6300 1922
Size: 30 2 30 4018
Fragments arranged by size:
4018 2227 543 401 380 193 163 88
65 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1941 5435 1941
Size: 3494 4902
HindIII A'AGCT_T
Cuts at: 4759 4759
Size: 8396
KasI G'GCGC_C
Cuts at: 1800 5826 6074 1800
Size: 4026 248 4122
Fragments arranged by size:
4122 4026 248
KpnI G_GTAC'C
Cuts at: 3512 5416 3512
Size: 1904 6492
MfeI C'AATT_G
Cuts at: 2748 2922 2748
Size: 174 8222
MluI A'CGCG_T
Cuts at: 1277 7829 1277
Size: 6552 1844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1560 2418 5160 7067 7251 1560
Size: 858 2742 1907 184 2705
Fragments arranged by size:
2742 2705 1907 858 184
MscI TGG'CCA
Cuts at: 626 914 1826 6157 626
Size: 288 912 4331 2865
Fragments arranged by size:
4331 2865 912 288
MslI CAynn'nnrTG
Cuts at: 1330 1607 2213 2309 2377 3541 6385 6512 1330
Size: 277 606 96 68 1164 2844 127 3214
Fragments arranged by size:
3214 2844 1164 606 277 127 96 68
MspA1I CmG'CkG
Cuts at: 1069 1106 1127 1172 1226 2002 2101 2447 2645
Size: 37 21 45 54 776 99 346 198
Cuts at: 2645 2684 3551 3569 4485 4783 4884 5507 5821
Size: 39 867 18 916 298 101 623 314
Cuts at: 5821 6181 7194 7439 1069
Size: 360 1013 245 2026
Fragments arranged by size:
2026 1013 916 867 776 623 360 346
314 298 245 198 101 99 54 45
39 37 21 18
NaeI GCC'GGC
Cuts at: 1368 6577 1368
Size: 5209 3187
NarI GG'CG_CC
Cuts at: 1801 5827 6075 1801
Size: 4026 248 4122
Fragments arranged by size:
4122 4026 248
NcoI C'CATG_G
Cuts at: 1608 2622 3200 1608
Size: 1014 578 6804
Fragments arranged by size:
6804 1014 578
NdeI CA'TA_TG
Cuts at: 339 1545 4691 339
Size: 1206 3146 4044
Fragments arranged by size:
4044 3146 1206
NgoMIV G'CCGG_C
Cuts at: 1366 6575 1366
Size: 5209 3187
NheI G'CTAG_C
Cuts at: 919 919
Size: 8396
NsiI A_TGCA'T
Cuts at: 3484 3484
Size: 8396
NspI r_CATG'y
Cuts at: 1699 2218 2382 3257 3546 5449 5949 6856 1699
Size: 519 164 875 289 1903 500 907 3239
Fragments arranged by size:
3239 1903 907 875 519 500 289 164
PasI CC'CwG_GG
Cuts at: 633 1455 1915 2427 633
Size: 822 460 512 6602
Fragments arranged by size:
6602 822 512 460
PciI A'CATG_T
Cuts at: 1695 2214 6852 1695
Size: 519 4638 3239
Fragments arranged by size:
4638 3239 519
PflMI CCAn_nnn'nTGG
Cuts at: 639 1729 1797 1867 2022 639
Size: 1090 68 70 155 7013
Fragments arranged by size:
7013 1090 155 70 68
PfoI T'CCnGG_A
Cuts at: 969 1046 1079 4242 969
Size: 77 33 3163 5123
Fragments arranged by size:
5123 3163 77 33
PmeI GTTT'AAAC
Cuts at: 5395 5395
Size: 8396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1847 1879 7535 7567 1847
Size: 32 5656 32 2676
Fragments arranged by size:
5656 2676 32 32
PpuMI rG'GwC_Cy
Cuts at: 1248 2335 3362 4321 1248
Size: 1087 1027 959 5323
Fragments arranged by size:
5323 1087 1027 959
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2135 2167 2135
Size: 32 8364
PstI C_TGCA'G
Cuts at: 1074 1231 2001 2275 2452 3061 3166 4925 5012
Size: 157 770 274 177 609 105 1759 87
Cuts at: 5012 5443 6128 1074
Size: 431 685 3342
Fragments arranged by size:
3342 1759 770 685 609 431 274 177
157 105 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8396
PvuII CAG'CTG
Cuts at: 1069 1106 2002 2447 2684 4485 4783 4884 5821
Size: 37 896 445 237 1801 298 101 937
Cuts at: 5821 6181 1069
Size: 360 3284
Fragments arranged by size:
3284 1801 937 896 445 360 298 237
101 37
RsrII CG'GwC_CG
Cuts at: 6591 6591
Size: 8396
SacI G_AGCT'C
Cuts at: 2908 5410 2908
Size: 2502 5894
SalI G'TCGA_C
Cuts at: 5433 5433
Size: 8396
SanDI GG'GwC_CC
Cuts at: 1248 4321 1248
Size: 3073 5323
SapI GCTCTTCn'nnn_
Cuts at: 6419 6629 6419
Size: 210 8186
SbfI CC_TGCA'GG
Cuts at: 5443 5443
Size: 8396
ScaI AGT'ACT
Cuts at: 7913 7913
Size: 8396
SexAI A'CCwGG_T
Cuts at: 3129 3129
Size: 8396
SfcI C'TryA_G
Cuts at: 33 1070 1227 1242 1997 2068 2271 2448 3057
Size: 1037 157 15 755 71 203 177 609
Cuts at: 3057 3162 4921 5008 5153 5385 5439 6124 7117
Size: 105 1759 87 145 232 54 685 993
Cuts at: 7117 7308 33
Size: 191 1121
Fragments arranged by size:
1759 1121 1037 993 755 685 609 232
203 191 177 157 145 105 87 71
54 15
SfoI GGC'GCC
Cuts at: 1802 5828 6076 1802
Size: 4026 248 4122
Fragments arranged by size:
4122 4026 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8396
SmaI CCC'GGG
Cuts at: 2351 3595 5418 2351
Size: 1244 1823 5329
Fragments arranged by size:
5329 1823 1244
SmlI C'TyrA_G
Cuts at: 164 1255 1926 2339 2570 2859 4853 5092 5551
Size: 1091 671 413 231 289 1994 239 459
Cuts at: 5551 6466 6958 7220 164
Size: 915 492 262 1340
Fragments arranged by size:
1994 1340 1091 915 671 492 459 413
289 262 239 231
SnaBI TAC'GTA
Cuts at: 1148 1148
Size: 8396
SpeI A'CTAG_T
Cuts at: 67 4090 67
Size: 4023 4373
SphI G_CATG'C
Cuts at: 5449 5949 5449
Size: 500 7896
SspI AAT'ATT
Cuts at: 580 2158 4048 4217 4800 5318 580
Size: 1578 1890 169 583 518 3658
Fragments arranged by size:
3658 1890 1578 583 518 169
StuI AGG'CCT
Cuts at: 3281 3281
Size: 8396
StyI C'CwwG_G
Cuts at: 138 813 1057 1485 1608 1791 2622 3200 3358
Size: 675 244 428 123 183 831 578 158
Cuts at: 3358 5530 5884 138
Size: 2172 354 2650
Fragments arranged by size:
2650 2172 831 675 578 428 354 244
183 158 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 131 1601 1680 2145 2567 5979 6112 6571 6780
Size: 1470 79 465 422 3412 133 459 209
Cuts at: 6780 131
Size: 1747
Fragments arranged by size:
3412 1747 1470 465 459 422 209 133
79
TatI w'GTAC_w
Cuts at: 2151 7911 2151
Size: 5760 2636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 591 623 2327 2359 7913 7945 591
Size: 32 1704 32 5554 32 1042
Fragments arranged by size:
5554 1704 1042 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2736 3241 6193 2736
Size: 505 2952 4939
Fragments arranged by size:
4939 2952 505
XbaI T'CTAG_A
Cuts at: 328 5427 328
Size: 5099 3297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1452 5080 5891 1452
Size: 3628 811 3957
Fragments arranged by size:
3957 3628 811
XmaI C'CCGG_G
Cuts at: 2349 3593 5416 2349
Size: 1244 1823 5329
Fragments arranged by size:
5329 1823 1244
XmnI GAAnn'nnTTC
Cuts at: 571 2811 3971 571
Size: 2240 1160 4996
Fragments arranged by size:
4996 2240 1160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
BaeI BmgBI BsiWI BssHII BstEII BstZ17I ClaI CspCI
DraIII FspAI HpaI NotI NruI PacI PmlI PshAI
PsiI PspOMI PspXI SacII SfiI SgrAI SrfI SwaI
XhoI ZraI