Restriction map for
pF1KE2754
(Circular) (Six-base) MAPSORT of: pF1KE2754.seq Check: 2314 from: 1 to: 3956
pF1KE2754 3956 bp
With 182 enzymes: SgfI *
June 30, 2019 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 595 595
Size: 3956
AccI GT'mk_AC
Cuts at: 994 994
Size: 3956
Acc65I G'GTAC_C
Cuts at: 878 972 878
Size: 94 3862
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 1781 2213 2960 3228 99
Size: 1682 432 747 268 827
Fragments arranged by size:
1682 827 747 432 268
AflIII A'CryG_T
Cuts at: 648 1308 2412 3389 648
Size: 660 1104 977 1215
Fragments arranged by size:
1215 1104 977 660
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 471 471
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 1261 2828 1261
Size: 1567 2389
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 478 595 960 3309 3565 478
Size: 117 365 2349 256 869
Fragments arranged by size:
2349 869 365 256 117
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 976 976
Size: 3956
AvrII C'CTAG_G
Cuts at: 228 228
Size: 3956
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 878 972 1386 1634 1669 878
Size: 94 414 248 35 3165
Fragments arranged by size:
3165 414 248 94 35
BanII G_rGCy'C
Cuts at: 970 2000 970
Size: 1030 2926
BbeI G_GCGC'C
Cuts at: 1390 1638 1390
Size: 248 3708
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 120 154 120
Size: 34 3922
BciVI GTATCCnnnnn_n'
Cuts at: 1854 2615 3956 1854
Size: 761 1341 1854
Fragments arranged by size:
1854 1341 761
BclI T'GATC_A
Cuts at: 1476 1476
Size: 3956
BglII A'GATC_T
Cuts at: 1471 3508 1471
Size: 2037 1919
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 1581 1674 2730 1581
Size: 93 1056 2807
Fragments arranged by size:
2807 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1168 1249 1584 3728 1168
Size: 81 335 2144 1396
Fragments arranged by size:
2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1132 2011 2503 2801 283
Size: 849 879 492 298 1438
Fragments arranged by size:
1438 879 849 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3660 3660
Size: 3956
BsaAI yAC'GTr
Cuts at: 451 1309 451
Size: 858 3098
BsaBI GATnn'nnATC
Cuts at: 6 170 1470 3507 6
Size: 164 1300 2037 455
Fragments arranged by size:
2037 1300 455 164
BsaHI Gr'CG_yC
Cuts at: 1387 1635 2337 3833 1387
Size: 248 702 1496 1510
Fragments arranged by size:
1510 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 3956
BseYI C'CCAG_C
Cuts at: 377 1381 2139 2716 3212 377
Size: 1004 758 577 496 1121
Fragments arranged by size:
1121 1004 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1498 1544 2752 104
Size: 1394 46 1208 1308
Fragments arranged by size:
1394 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 970 1748 1938 2730 970
Size: 778 190 792 2196
Fragments arranged by size:
2196 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 970 1581 1674 1748 1938 2000 2730 970
Size: 611 93 74 190 62 730 2196
Fragments arranged by size:
2196 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 463 595 1006 1220 1522 1903 463
Size: 132 411 214 302 381 2516
Fragments arranged by size:
2516 411 381 302 214 132
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 314 1868 314
Size: 1554 2402
BsrFI r'CCGG_y
Cuts at: 1954 2135 2344 1954
Size: 181 209 3566
Fragments arranged by size:
3566 209 181
BsrGI T'GTAC_A
Cuts at: 645 645
Size: 3956
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 1333
Size: 3956
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstEII G'GTnAC_C
Cuts at: 425 425
Size: 3956
BstYI r'GATC_y
Cuts at: 1 981 1448 1471 1806 2052 3064 3150 3162
Size: 980 467 23 335 246 1012 86 12
Cuts at: 3162 3508 1
Size: 346 449
Fragments arranged by size:
1012 980 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 571 107
Size: 464 3492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1906 2087 2250 1906
Size: 181 163 3612
Fragments arranged by size:
3612 181 163
BtsI GCAGTG_nn'
Cuts at: 1199 1199
Size: 3956
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 1541 1715 2106 2133 3478 1541
Size: 174 391 27 1345 2019
Fragments arranged by size:
2019 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1541 1541
Size: 3956
EarI CTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2164 2474 2620 3766 2164
Size: 310 146 1146 2354
Fragments arranged by size:
2354 1146 310 146
EcoICRI GAG'CTC
Cuts at: 968 968
Size: 3956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 1781 2213 2960 3228 99
Size: 1682 432 747 268 827
Fragments arranged by size:
1682 827 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1095 1095
Size: 3956
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3956
FspI TGC'GCA
Cuts at: 517 1737 517
Size: 1220 2736
HaeII r_GCGC'y
Cuts at: 1390 1638 2660 3639 1390
Size: 248 1022 979 1707
Fragments arranged by size:
1707 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 126 144 158 224 254 256 286 1798
Size: 14 18 14 66 30 2 30 1512
Cuts at: 1798 1828 1830 1860 112
Size: 30 2 30 2208
Fragments arranged by size:
2208 1512 66 30 30 30 30 18
14 14 2 2
HincII GTy'rAC
Cuts at: 414 995 414
Size: 581 3375
KasI G'GCGC_C
Cuts at: 1386 1634 1386
Size: 248 3708
KpnI G_GTAC'C
Cuts at: 882 976 882
Size: 94 3862
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 1717 1717
Size: 3956
MslI CAynn'nnrTG
Cuts at: 146 399 832 1945 2072 146
Size: 253 433 1113 127 2030
Fragments arranged by size:
2030 1113 433 253 127
MspA1I CmG'CkG
Cuts at: 643 1067 1381 1741 2754 2999 643
Size: 424 314 360 1013 245 1600
Fragments arranged by size:
1600 1013 424 360 314 245
NaeI GCC'GGC
Cuts at: 2137 2137
Size: 3956
NarI GG'CG_CC
Cuts at: 1387 1635 1387
Size: 248 3708
NcoI C'CATG_G
Cuts at: 107 107
Size: 3956
NgoMIV G'CCGG_C
Cuts at: 2135 2135
Size: 3956
NspI r_CATG'y
Cuts at: 652 1009 1509 2416 652
Size: 357 500 907 2192
Fragments arranged by size:
2192 907 500 357
PciI A'CATG_T
Cuts at: 648 2412 648
Size: 1764 2192
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PstI C_TGCA'G
Cuts at: 606 1003 1688 606
Size: 397 685 2874
Fragments arranged by size:
2874 685 397
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 643 1381 1741 643
Size: 738 360 2858
Fragments arranged by size:
2858 738 360
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 970 970
Size: 3956
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 335 3473 335
Size: 3138 818
SexAI A'CCwGG_T
Cuts at: 577 577
Size: 3956
SfcI C'TryA_G
Cuts at: 33 602 999 1684 2677 2868 33
Size: 569 397 685 993 191 1121
Fragments arranged by size:
1121 993 685 569 397 191
SfoI GGC'GCC
Cuts at: 1388 1636 1388
Size: 248 3708
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 978 978
Size: 3956
SmlI C'TyrA_G
Cuts at: 298 1111 2026 2518 2780 298
Size: 813 915 492 262 1474
Fragments arranged by size:
1474 915 813 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
SspI AAT'ATT
Cuts at: 724 724
Size: 3956
StyI C'CwwG_G
Cuts at: 107 228 257 1090 1444 107
Size: 121 29 833 354 2619
Fragments arranged by size:
2619 833 354 121 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 444 1539 1672 2131 2340 444
Size: 1095 133 459 209 2060
Fragments arranged by size:
2060 1095 459 209 133
TatI w'GTAC_w
Cuts at: 333 645 3471 333
Size: 312 2826 818
Fragments arranged by size:
2826 818 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 3473 3505 206
Size: 32 3235 32 657
Fragments arranged by size:
3235 657 32 32
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1451 1451
Size: 3956
XmaI C'CCGG_G
Cuts at: 976 976
Size: 3956
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI