Restriction map for
pF1KE2751
(Circular) (Six-base) MAPSORT of: pF1KE2751.seq Check: 7964 from: 1 to: 3929
pF1KE2751 3929 bp
With 182 enzymes: SgfI *
June 30, 2019 14:46 ..
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 215 3769 215
Size: 3554 375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1754 2186 2933 3201 641
Size: 1113 432 747 268 1369
Fragments arranged by size:
1369 1113 747 432 268
AflIII A'CryG_T
Cuts at: 601 921 1281 2385 3362 601
Size: 320 360 1104 977 1168
Fragments arranged by size:
1168 1104 977 360 320
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 357 389 357
Size: 32 3897
AlwNI CAG_nnn'CTG
Cuts at: 1234 2801 1234
Size: 1567 2362
ApaLI G'TGCA_C
Cuts at: 544 2699 544
Size: 2155 1774
ApoI r'AATT_y
Cuts at: 432 933 3282 3538 432
Size: 501 2349 256 823
Fragments arranged by size:
2349 823 501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 182 949 182
Size: 767 3162
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 191 945 1359 1607 1642 191
Size: 754 414 248 35 2478
Fragments arranged by size:
2478 754 414 248 35
BanII G_rGCy'C
Cuts at: 943 1973 943
Size: 1030 2899
BbeI G_GCGC'C
Cuts at: 195 1363 1611 195
Size: 1168 248 2513
Fragments arranged by size:
2513 1168 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3895
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BfrBI ATG'CAT
Cuts at: 207 207
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 236 236
Size: 3929
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 548 1554 1647 2703 548
Size: 1006 93 1056 1774
Fragments arranged by size:
1774 1056 1006 93
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 352 2296 352
Size: 1944 1985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2476 2774 1105
Size: 879 492 298 2260
Fragments arranged by size:
2260 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3633 3633
Size: 3929
BsaAI yAC'GTr
Cuts at: 1282 1282
Size: 3929
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 192 1360 1608 2310 3806 192
Size: 1168 248 702 1496 315
Fragments arranged by size:
1496 1168 702 315 248
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2591 2738 3149 1118
Size: 521 678 274 147 411 1898
Fragments arranged by size:
1898 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1354 2112 2689 3185 1354
Size: 758 577 496 2098
Fragments arranged by size:
2098 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 423
Size: 3929
BsiEI CG_ry'CG
Cuts at: 104 1471 1517 2725 104
Size: 1367 46 1208 1308
Fragments arranged by size:
1367 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 548 943 1721 1911 2703 548
Size: 395 778 190 792 1774
Fragments arranged by size:
1774 792 778 395 190
BsmI GAATG_Cn'
Cuts at: 209 209
Size: 3929
Bsp1286I G_dGCh'C
Cuts at: 548 943 1554 1647 1721 1911 1973 2703 548
Size: 395 611 93 74 190 62 730 1774
Fragments arranged by size:
1774 730 611 395 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 979 1193 1495 1876 979
Size: 214 302 381 3032
Fragments arranged by size:
3032 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2221 2275 3759 3924 50
Size: 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 1927 2108 2317 1927
Size: 181 209 3539
Fragments arranged by size:
3539 209 181
BsrGI T'GTAC_A
Cuts at: 391 703 918 391
Size: 312 215 3402
Fragments arranged by size:
3402 312 215
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 1306 1306
Size: 3929
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstEII G'GTnAC_C
Cuts at: 667 667
Size: 3929
BstYI r'GATC_y
Cuts at: 1 954 1421 1444 1779 2025 3037 3123 3135
Size: 953 467 23 335 246 1012 86 12
Cuts at: 3135 3481 1
Size: 346 449
Fragments arranged by size:
1012 953 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 1172 1172
Size: 3929
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 1514 1688 2079 2106 3451 1514
Size: 174 391 27 1345 1992
Fragments arranged by size:
1992 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1514 1514
Size: 3929
EarI CTCTTCn'nnn_
Cuts at: 345 1952 2162 345
Size: 1607 210 2112
Fragments arranged by size:
2112 1607 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2447 2593 3739 2137
Size: 310 146 1146 2327
Fragments arranged by size:
2327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1754 2186 2933 3201 641
Size: 1113 432 747 268 1369
Fragments arranged by size:
1369 1113 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 779 779
Size: 3929
EcoO109I rG'GnC_Cy
Cuts at: 1068 1068
Size: 3929
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
EcoRV GAT'ATC
Cuts at: 373 373
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 195 1363 1611 2633 3612 195
Size: 1168 248 1022 979 512
Fragments arranged by size:
1168 1022 979 512 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1771 1801 1803 1833 1771
Size: 30 2 30 3867
Fragments arranged by size:
3867 30 30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 191 1359 1607 191
Size: 1168 248 2513
Fragments arranged by size:
2513 1168 248
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MfeI C'AATT_G
Cuts at: 489 489
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 2600 2784 485
Size: 2115 184 1630
Fragments arranged by size:
2115 1630 184
MscI TGG'CCA
Cuts at: 1690 1690
Size: 3929
MslI CAynn'nnrTG
Cuts at: 1918 2045 1918
Size: 127 3802
MspA1I CmG'CkG
Cuts at: 1040 1354 1714 2727 2972 1040
Size: 314 360 1013 245 1997
Fragments arranged by size:
1997 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2110 2110
Size: 3929
NarI GG'CG_CC
Cuts at: 192 1360 1608 192
Size: 1168 248 2513
Fragments arranged by size:
2513 1168 248
NgoMIV G'CCGG_C
Cuts at: 2108 2108
Size: 3929
NsiI A_TGCA'T
Cuts at: 209 209
Size: 3929
NspI r_CATG'y
Cuts at: 251 605 982 1482 2389 251
Size: 354 377 500 907 1791
Fragments arranged by size:
1791 907 500 377 354
PciI A'CATG_T
Cuts at: 601 2385 601
Size: 1784 2145
PflMI CCAn_nnn'nTGG
Cuts at: 525 525
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PstI C_TGCA'G
Cuts at: 976 1661 976
Size: 685 3244
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 1354 1714 1354
Size: 360 3569
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 972 1657 2650 2841 33
Size: 939 685 993 191 1121
Fragments arranged by size:
1121 993 939 685 191
SfoI GGC'GCC
Cuts at: 193 1361 1609 193
Size: 1168 248 2513
Fragments arranged by size:
2513 1168 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 184 951 184
Size: 767 3162
SmlI C'TyrA_G
Cuts at: 1084 1999 2491 2753 1084
Size: 915 492 262 2260
Fragments arranged by size:
2260 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 231 471 571 1063 1417 231
Size: 240 100 492 354 2743
Fragments arranged by size:
2743 492 354 240 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 1645 2104 2313 1512
Size: 133 459 209 3128
Fragments arranged by size:
3128 459 209 133
TatI w'GTAC_w
Cuts at: 391 703 918 3444 391
Size: 312 215 2526 876
Fragments arranged by size:
2526 876 312 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1424 1424
Size: 3929
XmaI C'CCGG_G
Cuts at: 182 949 182
Size: 767 3162
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BbvCI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmBI BssHII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII FseI FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI