Restriction map for
pF1KE2747
(Circular) (Six-base) MAPSORT of: pF1KE2747.seq Check: 1596 from: 1 to: 5711
pF1KE2747 5711 bp
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 193 193
Size: 5711
AccI GT'mk_AC
Cuts at: 1356 2749 1356
Size: 1393 4318
Acc65I G'GTAC_C
Cuts at: 1262 2727 1262
Size: 1465 4246
AclI AA'CG_TT
Cuts at: 5551 5551
Size: 5711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 3536 3968 4715 4983 251
Size: 3285 432 747 268 979
Fragments arranged by size:
3285 979 747 432 268
AflIII A'CryG_T
Cuts at: 966 3063 4167 5144 966
Size: 2097 1104 977 1533
Fragments arranged by size:
2097 1533 1104 977
AgeI A'CCGG_T
Cuts at: 4099 4099
Size: 5711
AlwNI CAG_nnn'CTG
Cuts at: 3016 4583 3016
Size: 1567 4144
ApaLI G'TGCA_C
Cuts at: 1088 4481 1088
Size: 3393 2318
ApoI r'AATT_y
Cuts at: 827 1372 2047 2715 5064 5320 827
Size: 545 675 668 2349 256 1218
Fragments arranged by size:
2349 1218 675 668 545 256
AseI AT'TA_AT
Cuts at: 20 541 694 2159 20
Size: 521 153 1465 3572
Fragments arranged by size:
3572 1465 521 153
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
AvaI C'yCGr_G
Cuts at: 2297 2518 2731 2297
Size: 221 213 5277
Fragments arranged by size:
5277 221 213
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1246 1279 1246
Size: 33 5678
BamHI G'GATC_C
Cuts at: 2736 2736
Size: 5711
BanI G'GyrC_C
Cuts at: 1262 2550 2727 3141 3389 3424 1262
Size: 1288 177 414 248 35 3549
Fragments arranged by size:
3549 1288 414 248 177 35
BanII G_rGCy'C
Cuts at: 2383 2725 3755 2383
Size: 342 1030 4339
Fragments arranged by size:
4339 1030 342
BbeI G_GCGC'C
Cuts at: 2554 3145 3393 2554
Size: 591 248 4872
Fragments arranged by size:
4872 591 248
BbvCI CC'TCA_GC
Cuts at: 1059 1059
Size: 5711
BciVI GTATCCnnnnn_n'
Cuts at: 1394 2014 3609 4370 5711 1394
Size: 620 1595 761 1341 1394
Fragments arranged by size:
1595 1394 1341 761 620
BclI T'GATC_A
Cuts at: 1472 3231 1472
Size: 1759 3952
BglI GCCn_nnn'nGGC
Cuts at: 2559 2603 2559
Size: 44 5667
BglII A'GATC_T
Cuts at: 3226 5263 3226
Size: 2037 3674
BlpI GC'TnA_GC
Cuts at: 2823 2823
Size: 5711
Bme1580I G_kGCm'C
Cuts at: 531 1092 3336 3429 4485 531
Size: 561 2244 93 1056 1757
Fragments arranged by size:
2244 1757 1056 561 93
BmgBI CAC'GTC
Cuts at: 2538 2538
Size: 5711
BmrI ACTGGGnnnn_n'
Cuts at: 2923 3004 3339 5483 2923
Size: 81 335 2144 3151
Fragments arranged by size:
3151 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4078 4078
Size: 5711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1931 1931
Size: 5711
Bpu10I CC'TnA_GC
Cuts at: 1059 1854 2106 1059
Size: 795 252 4664
Fragments arranged by size:
4664 795 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 783 2887 3766 4258 4556 783
Size: 2104 879 492 298 1938
Fragments arranged by size:
2104 1938 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2076 2231 5415 2076
Size: 155 3184 2372
Fragments arranged by size:
3184 2372 155
BsaAI yAC'GTr
Cuts at: 3064 3064
Size: 5711
BsaBI GATnn'nnATC
Cuts at: 6 519 3225 5262 6
Size: 513 2706 2037 455
Fragments arranged by size:
2706 2037 513 455
BsaHI Gr'CG_yC
Cuts at: 2551 3142 3390 4092 5588 2551
Size: 591 248 702 1496 2674
Fragments arranged by size:
2674 1496 702 591 248
BsaWI w'CCGG_w
Cuts at: 2900 3421 4099 4373 4520 4931 2900
Size: 521 678 274 147 411 3680
Fragments arranged by size:
3680 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 191 221 191
Size: 30 5681
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1115 2668 1115
Size: 1553 4158
BseYI C'CCAG_C
Cuts at: 1693 2439 3136 3894 4471 4967 1693
Size: 746 697 758 577 496 2437
Fragments arranged by size:
2437 758 746 697 577 496
BsiEI CG_ry'CG
Cuts at: 104 3253 3299 4507 104
Size: 3149 46 1208 1308
Fragments arranged by size:
3149 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1092 2725 3503 3693 4485 1092
Size: 1633 778 190 792 2318
Fragments arranged by size:
2318 1633 792 778 190
BsmI GAATG_Cn'
Cuts at: 1548 2366 1548
Size: 818 4893
Bsp1286I G_dGCh'C
Cuts at: 531 1092 2383 2725 3336 3429 3503 3693 3755
Size: 561 1291 342 611 93 74 190 62
Cuts at: 3755 4485 531
Size: 730 1757
Fragments arranged by size:
1757 1291 730 611 561 342 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4931 4931
Size: 5711
BspHI T'CATG_A
Cuts at: 731 1949 4887 731
Size: 1218 2938 1555
Fragments arranged by size:
2938 1555 1218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 193 2761 2975 3277 3658 193
Size: 2568 214 302 381 2246
Fragments arranged by size:
2568 2246 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4003 4057 5541 5706 50
Size: 3953 54 1484 165 55
Fragments arranged by size:
3953 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 548 3623 548
Size: 3075 2636
BsrFI r'CCGG_y
Cuts at: 3709 3890 4099 3709
Size: 181 209 5321
Fragments arranged by size:
5321 209 181
BsrGI T'GTAC_A
Cuts at: 746 746
Size: 5711
BssSI C'ACGA_G
Cuts at: 3982 4340 3982
Size: 358 5353
BstAPI GCAn_nnn'nTGC
Cuts at: 1342 3088 1342
Size: 1746 3965
BstBI TT'CG_AA
Cuts at: 2361 4072 2361
Size: 1711 4000
BstYI r'GATC_y
Cuts at: 1 324 631 2736 3203 3226 3561 3807 4819
Size: 323 307 2105 467 23 335 246 1012
Cuts at: 4819 4905 4917 5263 1
Size: 86 12 346 449
Fragments arranged by size:
2105 1012 467 449 346 335 323 307
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1258 1832 107
Size: 1151 574 3986
Fragments arranged by size:
3986 1151 574
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1698 3661 3842 4005 1698
Size: 1963 181 163 3404
Fragments arranged by size:
3404 1963 181 163
BtsI GCAGTG_nn'
Cuts at: 257 2458 2954 257
Size: 2201 496 3014
Fragments arranged by size:
3014 2201 496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1356 1391 1356
Size: 35 5676
DraI TTT'AAA
Cuts at: 1635 2710 1635
Size: 1075 4636
DrdI GACnn_nn'nnGTC
Cuts at: 3417 4275 3417
Size: 858 4853
EaeI y'GGCC_r
Cuts at: 533 1700 2347 3296 3470 3861 3888 5233 533
Size: 1167 647 949 174 391 27 1345 1011
Fragments arranged by size:
1345 1167 1011 949 647 391 174 27
EagI C'GGCC_G
Cuts at: 3296 3296
Size: 5711
EarI CTCTTCn'nnn_
Cuts at: 647 1115 1518 2229 2333 2666 3734 3944 647
Size: 468 403 711 104 333 1068 210 2414
Fragments arranged by size:
2414 1068 711 468 403 333 210 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3919 4229 4375 5521 3919
Size: 310 146 1146 4109
Fragments arranged by size:
4109 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2723 2723
Size: 5711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 1931 3536 3968 4715 4983 251
Size: 1680 1605 432 747 268 979
Fragments arranged by size:
1680 1605 979 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 577 793 1120 1980 2119 577
Size: 216 327 860 139 4169
Fragments arranged by size:
4169 860 327 216 139
EcoO109I rG'GnC_Cy
Cuts at: 715 955 2850 715
Size: 240 1895 3576
Fragments arranged by size:
3576 1895 240
EcoRI G'AATT_C
Cuts at: 827 2047 2715 827
Size: 1220 668 3823
Fragments arranged by size:
3823 1220 668
EcoRV GAT'ATC
Cuts at: 2581 2581
Size: 5711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3730 3730
Size: 5711
FspI TGC'GCA
Cuts at: 3492 3492
Size: 5711
HaeII r_GCGC'y
Cuts at: 2554 3145 3393 4415 5394 2554
Size: 591 248 1022 979 2871
Fragments arranged by size:
2871 1022 979 591 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 469 501 848 880 1033 1065 2171 2203 2213
Size: 32 347 32 153 32 1106 32 10
Cuts at: 2213 2245 3553 3583 3585 3615 469
Size: 32 1308 30 2 30 2565
Fragments arranged by size:
2565 1308 1106 347 153 32 32 32
32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 1357 2750 1357
Size: 1393 4318
HindIII A'AGCT_T
Cuts at: 2356 2356
Size: 5711
KasI G'GCGC_C
Cuts at: 2550 3141 3389 2550
Size: 591 248 4872
Fragments arranged by size:
4872 591 248
KpnI G_GTAC'C
Cuts at: 1266 2731 1266
Size: 1465 4246
MfeI C'AATT_G
Cuts at: 1094 2246 1094
Size: 1152 4559
MluI A'CGCG_T
Cuts at: 5144 5144
Size: 5711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 246 1947 4382 4566 246
Size: 1701 2435 184 1391
Fragments arranged by size:
2435 1701 1391 184
MscI TGG'CCA
Cuts at: 1702 3472 1702
Size: 1770 3941
MslI CAynn'nnrTG
Cuts at: 2407 3700 3827 2407
Size: 1293 127 4291
Fragments arranged by size:
4291 1293 127
MspA1I CmG'CkG
Cuts at: 123 1054 1063 2822 3136 3496 4509 4754 123
Size: 931 9 1759 314 360 1013 245 1080
Fragments arranged by size:
1759 1080 1013 931 360 314 245 9
NaeI GCC'GGC
Cuts at: 3892 3892
Size: 5711
NarI GG'CG_CC
Cuts at: 2551 3142 3390 2551
Size: 591 248 4872
Fragments arranged by size:
4872 591 248
NcoI C'CATG_G
Cuts at: 107 1258 1832 107
Size: 1151 574 3986
Fragments arranged by size:
3986 1151 574
NdeI CA'TA_TG
Cuts at: 360 810 2592 360
Size: 450 1782 3479
Fragments arranged by size:
3479 1782 450
NgoMIV G'CCGG_C
Cuts at: 3890 3890
Size: 5711
NspI r_CATG'y
Cuts at: 970 1853 2764 3264 4171 970
Size: 883 911 500 907 2510
Fragments arranged by size:
2510 911 907 883 500
PciI A'CATG_T
Cuts at: 966 4167 966
Size: 3201 2510
PflMI CCAn_nnn'nTGG
Cuts at: 352 1961 352
Size: 1609 4102
PmeI GTTT'AAAC
Cuts at: 2710 2710
Size: 5711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 109 141 4850 4882 109
Size: 32 4709 32 938
Fragments arranged by size:
4709 938 32 32
PpuMI rG'GwC_Cy
Cuts at: 715 955 715
Size: 240 5471
PshAI GACnn'nnGTC
Cuts at: 1362 1496 1362
Size: 134 5577
PsiI TTA'TAA
Cuts at: 2567 2567
Size: 5711
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 261 293 261
Size: 32 5679
PstI C_TGCA'G
Cuts at: 264 1068 1947 2758 3443 264
Size: 804 879 811 685 2532
Fragments arranged by size:
2532 879 811 804 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5711
PvuII CAG'CTG
Cuts at: 123 1054 1063 3136 3496 123
Size: 931 9 2073 360 2338
Fragments arranged by size:
2338 2073 931 360 9
RsrII CG'GwC_CG
Cuts at: 3906 3906
Size: 5711
SacI G_AGCT'C
Cuts at: 2725 2725
Size: 5711
SalI G'TCGA_C
Cuts at: 1355 2748 1355
Size: 1393 4318
SapI GCTCTTCn'nnn_
Cuts at: 3734 3944 3734
Size: 210 5501
SbfI CC_TGCA'GG
Cuts at: 2758 2758
Size: 5711
ScaI AGT'ACT
Cuts at: 5228 5228
Size: 5711
SfcI C'TryA_G
Cuts at: 33 141 260 1064 1076 1527 1943 2754 3439
Size: 108 119 804 12 451 416 811 685
Cuts at: 3439 4432 4623 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 811 804 685 451 416 191
119 108 12
SfoI GGC'GCC
Cuts at: 2552 3143 3391 2552
Size: 591 248 4872
Fragments arranged by size:
4872 591 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
SmaI CCC'GGG
Cuts at: 2733 2733
Size: 5711
SmlI C'TyrA_G
Cuts at: 762 2518 2866 3781 4273 4535 762
Size: 1756 348 915 492 262 1938
Fragments arranged by size:
1938 1756 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5711
SphI G_CATG'C
Cuts at: 1853 2764 3264 1853
Size: 911 500 4300
Fragments arranged by size:
4300 911 500
SspI AAT'ATT
Cuts at: 1341 1341
Size: 5711
StyI C'CwwG_G
Cuts at: 107 1258 1832 2546 2557 2845 3199 107
Size: 1151 574 714 11 288 354 2619
Fragments arranged by size:
2619 1151 714 574 354 288 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 375 3294 3427 3886 4095 375
Size: 2919 133 459 209 1991
Fragments arranged by size:
2919 1991 459 209 133
TatI w'GTAC_w
Cuts at: 746 5226 746
Size: 4480 1231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1712 1744 5228 5260 1712
Size: 32 3484 32 2163
Fragments arranged by size:
3484 2163 32 32
Tth111I GACn'n_nGTC
Cuts at: 3508 3508
Size: 5711
XbaI T'CTAG_A
Cuts at: 2742 2742
Size: 5711
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3206 3206
Size: 5711
XhoI C'TCGA_G
Cuts at: 2518 2518
Size: 5711
XmaI C'CCGG_G
Cuts at: 2731 2731
Size: 5711
XmnI GAAnn'nnTTC
Cuts at: 341 1277 341
Size: 936 4775
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BcgI BfrBI BmtI BsgI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI