Restriction map for
pF1KE2742
(Circular) (Six-base) MAPSORT of: pF1KE2742.seq Check: 5645 from: 1 to: 7196
pF1KE2742 7196 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AatII G_ACGT'C
Cuts at: 2990 2990
Size: 7196
AccI GT'mk_AC
Cuts at: 1235 1802 2990 3680 4234 1235
Size: 567 1188 690 554 4197
Fragments arranged by size:
4197 1188 690 567 554
Acc65I G'GTAC_C
Cuts at: 4212 4212
Size: 7196
AclI AA'CG_TT
Cuts at: 7036 7036
Size: 7196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 932 1759 2446 3430 3547 3882 5021 5453
Size: 385 827 687 984 117 335 1139 432
Cuts at: 5453 6200 6468 547
Size: 747 268 1275
Fragments arranged by size:
1275 1139 984 827 747 687 432 385
335 268 117
AflIII A'CryG_T
Cuts at: 4548 5652 6629 4548
Size: 1104 977 5115
Fragments arranged by size:
5115 1104 977
AgeI A'CCGG_T
Cuts at: 722 3240 5584 722
Size: 2518 2344 2334
Fragments arranged by size:
2518 2344 2334
AleI CACnn'nnGTG
Cuts at: 617 1360 617
Size: 743 6453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 313 313
Size: 7196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 662 694 749 781 662
Size: 32 55 32 7077
Fragments arranged by size:
7077 55 32 32
AlwNI CAG_nnn'CTG
Cuts at: 205 1286 1490 1758 2176 3635 4501 6068 205
Size: 1081 204 268 418 1459 866 1567 1333
Fragments arranged by size:
1567 1459 1333 1081 866 418 268 204
ApaI G_GGCC'C
Cuts at: 2224 2677 2936 2224
Size: 453 259 6484
Fragments arranged by size:
6484 453 259
ApaLI G'TGCA_C
Cuts at: 5966 5966
Size: 7196
ApoI r'AATT_y
Cuts at: 1133 2275 2721 2738 4200 6549 6805 1133
Size: 1142 446 17 1462 2349 256 1524
Fragments arranged by size:
2349 1524 1462 1142 446 256 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 7196
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7196
AvaI C'yCGr_G
Cuts at: 998 2432 4216 998
Size: 1434 1784 3978
Fragments arranged by size:
3978 1784 1434
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1409 1442 1513 1546 1409
Size: 33 71 33 7059
Fragments arranged by size:
7059 71 33 33
BamHI G'GATC_C
Cuts at: 510 797 3338 4221 510
Size: 287 2541 883 3485
Fragments arranged by size:
3485 2541 883 287
BanI G'GyrC_C
Cuts at: 212 783 3053 3367 4212 4626 4874 4909 212
Size: 571 2270 314 845 414 248 35 2499
Fragments arranged by size:
2499 2270 845 571 414 314 248 35
BanII G_rGCy'C
Cuts at: 229 2224 2677 2936 3353 4210 5240 229
Size: 1995 453 259 417 857 1030 2185
Fragments arranged by size:
2185 1995 1030 857 453 417 259
BbeI G_GCGC'C
Cuts at: 4630 4878 4630
Size: 248 6948
BbsI GAAGACnn'nnnn_
Cuts at: 962 3426 962
Size: 2464 4732
BbvCI CC'TCA_GC
Cuts at: 1219 1219
Size: 7196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 369 403 2335 2369 369
Size: 34 1932 34 5196
Fragments arranged by size:
5196 1932 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5094 5855 7196 5094
Size: 761 1341 5094
Fragments arranged by size:
5094 1341 761
BclI T'GATC_A
Cuts at: 1904 2057 2318 4716 1904
Size: 153 261 2398 4384
Fragments arranged by size:
4384 2398 261 153
BglI GCCn_nnn'nGGC
Cuts at: 856 1329 2681 856
Size: 473 1352 5371
Fragments arranged by size:
5371 1352 473
BglII A'GATC_T
Cuts at: 4711 6748 4711
Size: 2037 5159
BlpI GC'TnA_GC
Cuts at: 4308 4308
Size: 7196
Bme1580I G_kGCm'C
Cuts at: 217 444 788 1732 2224 2677 2936 4163 4821
Size: 227 344 944 492 453 259 1227 658
Cuts at: 4821 4914 5970 217
Size: 93 1056 1443
Fragments arranged by size:
1443 1227 1056 944 658 492 453 344
259 227 93
BmgBI CAC'GTC
Cuts at: 1074 3684 1074
Size: 2610 4586
BmrI ACTGGGnnnn_n'
Cuts at: 2278 3285 3507 3821 4408 4489 4824 6968 2278
Size: 1007 222 314 587 81 335 2144 2506
Fragments arranged by size:
2506 2144 1007 587 335 314 222 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1234 3367 5563 1234
Size: 2133 2196 2867
Fragments arranged by size:
2867 2196 2133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1433 2821 3102 3217 3987 1433
Size: 1388 281 115 770 4642
Fragments arranged by size:
4642 1388 770 281 115
Bpu10I CC'TnA_GC
Cuts at: 1219 1219
Size: 7196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 4039 4372 5251 5743 6041 580
Size: 3459 333 879 492 298 1735
Fragments arranged by size:
3459 1735 879 492 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 4057 6900 4057
Size: 2843 4353
BsaAI yAC'GTr
Cuts at: 2211 4549 2211
Size: 2338 4858
BsaBI GATnn'nnATC
Cuts at: 6 4710 6747 6
Size: 4704 2037 455
Fragments arranged by size:
4704 2037 455
BsaHI Gr'CG_yC
Cuts at: 2414 2987 3431 3626 4627 4875 5577 7073 2414
Size: 573 444 195 1001 248 702 1496 2537
Fragments arranged by size:
2537 1496 1001 702 573 444 248 195
BsaWI w'CCGG_w
Cuts at: 722 3240 4385 4906 5584 5858 6005 6416 722
Size: 2518 1145 521 678 274 147 411 1502
Fragments arranged by size:
2518 1502 1145 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 662 692 1284 1314 2575 2605 3001 3031 662
Size: 30 592 30 1261 30 396 30 4827
Fragments arranged by size:
4827 1261 592 396 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 434 1561 2011 434
Size: 1127 450 5619
Fragments arranged by size:
5619 1127 450
BseYI C'CCAG_C
Cuts at: 118 208 433 656 3178 4621 5379 5956 6452
Size: 90 225 223 2522 1443 758 577 496
Cuts at: 6452 118
Size: 862
Fragments arranged by size:
2522 1443 862 758 577 496 225 223
90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1519 2760 3621 874
Size: 645 1241 861 4449
Fragments arranged by size:
4449 1241 861 645
BsiEI CG_ry'CG
Cuts at: 104 1275 2371 4738 4784 5992 104
Size: 1171 1096 2367 46 1208 1308
Fragments arranged by size:
2367 1308 1208 1171 1096 46
BsiHKAI G_wGCw'C
Cuts at: 2261 2295 3220 4210 4988 5178 5970 2261
Size: 34 925 990 778 190 792 3487
Fragments arranged by size:
3487 990 925 792 778 190 34
BsmI GAATG_Cn'
Cuts at: 4075 4075
Size: 7196
BsmBI CGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 7196
Bsp1286I G_dGCh'C
Cuts at: 217 229 444 788 1732 2224 2261 2295 2677
Size: 12 215 344 944 492 37 34 382
Cuts at: 2677 2936 3220 3353 4163 4210 4821 4914 4988
Size: 259 284 133 810 47 611 93 74
Cuts at: 4988 5178 5240 5970 217
Size: 190 62 730 1443
Fragments arranged by size:
1443 944 810 730 611 492 382 344
284 259 215 190 133 93 74 62
47 37 34 12
BspEI T'CCGG_A
Cuts at: 6416 6416
Size: 7196
BspHI T'CATG_A
Cuts at: 1098 6372 1098
Size: 5274 1922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 184 1541 3155 4246 4460 4762 5143 184
Size: 1357 1614 1091 214 302 381 2237
Fragments arranged by size:
2237 1614 1357 1091 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5488 5542 7026 7191 50
Size: 5438 54 1484 165 55
Fragments arranged by size:
5438 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1427 2115 2459 2879 5108 1427
Size: 688 344 420 2229 3515
Fragments arranged by size:
3515 2229 688 420 344
BsrFI r'CCGG_y
Cuts at: 722 1792 2439 2525 2807 2875 3240 5194 5375
Size: 1070 647 86 282 68 365 1954 181
Cuts at: 5375 5584 722
Size: 209 2334
Fragments arranged by size:
2334 1954 1070 647 365 282 209 181
86 68
BsrGI T'GTAC_A
Cuts at: 1395 3119 1395
Size: 1724 5472
BssSI C'ACGA_G
Cuts at: 3891 5467 5825 3891
Size: 1576 358 5262
Fragments arranged by size:
5262 1576 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1286 1490 2048 3104 4573 1286
Size: 204 558 1056 1469 3909
Fragments arranged by size:
3909 1469 1056 558 204
BstBI TT'CG_AA
Cuts at: 5557 5557
Size: 7196
BstXI CCAn_nnnn'nTGG
Cuts at: 1203 1993 2823 4026 4170 1203
Size: 790 830 1203 144 4229
Fragments arranged by size:
4229 1203 830 790 144
BstYI r'GATC_y
Cuts at: 1 510 739 797 1486 2173 2666 3338 4221
Size: 509 229 58 689 687 493 672 883
Cuts at: 4221 4688 4711 5046 5292 6304 6390 6402 6748
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6748 1
Size: 449
Fragments arranged by size:
1012 883 689 687 672 509 493 467
449 346 335 246 229 86 58 23
12
BtgI C'CryG_G
Cuts at: 107 317 666 1884 2228 2481 3925 4012 107
Size: 210 349 1218 344 253 1444 87 3291
Fragments arranged by size:
3291 1444 1218 349 344 253 210 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 874 5146 5327 5490 874
Size: 4272 181 163 2580
Fragments arranged by size:
4272 2580 181 163
BtsI GCAGTG_nn'
Cuts at: 295 818 1938 3105 4439 295
Size: 523 1120 1167 1334 3052
Fragments arranged by size:
3052 1334 1167 1120 523
DraI TTT'AAA
Cuts at: 4195 4195
Size: 7196
DraIII CAC_nnn'GTG
Cuts at: 273 273
Size: 7196
DrdI GACnn_nn'nnGTC
Cuts at: 2332 4902 5760 2332
Size: 2570 858 3768
Fragments arranged by size:
3768 2570 858
EaeI y'GGCC_r
Cuts at: 252 282 848 1790 2132 2368 2441 2558 4781
Size: 30 566 942 342 236 73 117 2223
Cuts at: 4781 4955 5346 5373 6718 252
Size: 174 391 27 1345 730
Fragments arranged by size:
2223 1345 942 730 566 391 342 236
174 117 73 30 27
EagI C'GGCC_G
Cuts at: 2368 4781 2368
Size: 2413 4783
EarI CTCTTCn'nnn_
Cuts at: 142 315 1457 5219 5429 142
Size: 173 1142 3762 210 1909
Fragments arranged by size:
3762 1909 1142 210 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1588 2821 5404 5714 5860 7006 1588
Size: 1233 2583 310 146 1146 1778
Fragments arranged by size:
2583 1778 1233 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4208 4208
Size: 7196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 932 1433 1759 2446 2821 3102 3217 3430
Size: 385 501 326 687 375 281 115 213
Cuts at: 3430 3547 3882 3987 5021 5453 6200 6468 547
Size: 117 335 105 1034 432 747 268 1275
Fragments arranged by size:
1275 1034 747 687 501 432 385 375
335 326 281 268 213 117 115 105
EcoNI CCTnn'n_nnAGG
Cuts at: 139 3256 139
Size: 3117 4079
EcoO109I rG'GnC_Cy
Cuts at: 1842 2221 2486 2673 2674 2702 2933 3443 4335
Size: 379 265 187 1 28 231 510 892
Cuts at: 4335 1842
Size: 4703
Fragments arranged by size:
4703 892 510 379 265 231 187 28
1
EcoRI G'AATT_C
Cuts at: 2738 4200 2738
Size: 1462 5734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2631 3189 3720 3786 5215 2631
Size: 558 531 66 1429 4612
Fragments arranged by size:
4612 1429 558 531 66
FspI TGC'GCA
Cuts at: 4977 4977
Size: 7196
HaeII r_GCGC'y
Cuts at: 557 2822 3208 4630 4878 5900 6879 557
Size: 2265 386 1422 248 1022 979 874
Fragments arranged by size:
2265 1422 1022 979 874 386 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 682 714 744 776 1093 1125 2121 2153 2575
Size: 32 30 32 317 32 996 32 422
Cuts at: 2575 2607 2956 2988 3493 3525 3588 3620 5038
Size: 32 349 32 505 32 63 32 1418
Cuts at: 5038 5068 5070 5100 682
Size: 30 2 30 2778
Fragments arranged by size:
2778 1418 996 505 422 349 317 63
32 32 32 32 32 32 32 32
30 30 30 2
HincII GTy'rAC
Cuts at: 1215 2991 4235 1215
Size: 1776 1244 4176
Fragments arranged by size:
4176 1776 1244
HindIII A'AGCT_T
Cuts at: 4033 4033
Size: 7196
HpaI GTT'AAC
Cuts at: 1215 1215
Size: 7196
KasI G'GCGC_C
Cuts at: 4626 4874 4626
Size: 248 6948
KpnI G_GTAC'C
Cuts at: 4216 4216
Size: 7196
MluI A'CGCG_T
Cuts at: 6629 6629
Size: 7196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 5867 6051 2324
Size: 3543 184 3469
Fragments arranged by size:
3543 3469 184
MscI TGG'CCA
Cuts at: 254 850 2134 2560 4957 254
Size: 596 1284 426 2397 2493
Fragments arranged by size:
2493 2397 1284 596 426
MslI CAynn'nnrTG
Cuts at: 617 1201 1360 1603 1606 3805 3847 4111 4168
Size: 584 159 243 3 2199 42 264 57
Cuts at: 4168 5185 5312 617
Size: 1017 127 2501
Fragments arranged by size:
2501 2199 1017 584 264 243 159 127
57 42 3
MspA1I CmG'CkG
Cuts at: 161 1758 2167 2424 2522 2949 3717 4307 4621
Size: 1597 409 257 98 427 768 590 314
Cuts at: 4621 4981 5994 6239 161
Size: 360 1013 245 1118
Fragments arranged by size:
1597 1118 1013 768 590 427 409 360
314 257 245 98
NaeI GCC'GGC
Cuts at: 2441 2809 5377 2441
Size: 368 2568 4260
Fragments arranged by size:
4260 2568 368
NarI GG'CG_CC
Cuts at: 4627 4875 4627
Size: 248 6948
NcoI C'CATG_G
Cuts at: 107 1884 3925 4012 107
Size: 1777 2041 87 3291
Fragments arranged by size:
3291 2041 1777 87
NdeI CA'TA_TG
Cuts at: 1745 1745
Size: 7196
NgoMIV G'CCGG_C
Cuts at: 2439 2807 5375 2439
Size: 368 2568 4260
Fragments arranged by size:
4260 2568 368
NspI r_CATG'y
Cuts at: 433 1569 4249 4749 5656 433
Size: 1136 2680 500 907 1973
Fragments arranged by size:
2680 1973 1136 907 500
PasI CC'CwG_GG
Cuts at: 184 2077 184
Size: 1893 5303
PciI A'CATG_T
Cuts at: 5652 5652
Size: 7196
PflMI CCAn_nnn'nTGG
Cuts at: 161 1061 2126 3275 3738 3852 161
Size: 900 1065 1149 463 114 3505
Fragments arranged by size:
3505 1149 1065 900 463 114
PfoI T'CCnGG_A
Cuts at: 586 586
Size: 7196
PmeI GTTT'AAAC
Cuts at: 4195 4195
Size: 7196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 662 694 6335 6367 662
Size: 32 5641 32 1491
Fragments arranged by size:
5641 1491 32 32
PpuMI rG'GwC_Cy
Cuts at: 1842 2702 3443 1842
Size: 860 741 5595
Fragments arranged by size:
5595 860 741
PshAI GACnn'nnGTC
Cuts at: 1988 3430 4049 1988
Size: 1442 619 5135
Fragments arranged by size:
5135 1442 619
PspOMI G'GGCC_C
Cuts at: 2220 2673 2932 2220
Size: 453 259 6484
Fragments arranged by size:
6484 453 259
PstI C_TGCA'G
Cuts at: 1486 2172 3257 4243 4928 1486
Size: 686 1085 986 685 3754
Fragments arranged by size:
3754 1085 986 686 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7196
PvuII CAG'CTG
Cuts at: 161 1758 2522 2949 3717 4621 4981 161
Size: 1597 764 427 768 904 360 2376
Fragments arranged by size:
2376 1597 904 768 764 427 360
RsrII CG'GwC_CG
Cuts at: 2031 5391 2031
Size: 3360 3836
SacI G_AGCT'C
Cuts at: 4210 4210
Size: 7196
SalI G'TCGA_C
Cuts at: 2989 4233 2989
Size: 1244 5952
SapI GCTCTTCn'nnn_
Cuts at: 5219 5429 5219
Size: 210 6986
SbfI CC_TGCA'GG
Cuts at: 4243 4243
Size: 7196
ScaI AGT'ACT
Cuts at: 1437 6713 1437
Size: 5276 1920
SexAI A'CCwGG_T
Cuts at: 1502 1502
Size: 7196
SfcI C'TryA_G
Cuts at: 33 735 1482 1638 2168 2711 3253 4239 4924
Size: 702 747 156 530 543 542 986 685
Cuts at: 4924 5917 6108 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 986 747 702 685 543 542
530 191 156
SfoI GGC'GCC
Cuts at: 4628 4876 4628
Size: 248 6948
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7196
SmaI CCC'GGG
Cuts at: 1000 2434 4218 1000
Size: 1434 1784 3978
Fragments arranged by size:
3978 1784 1434
SmlI C'TyrA_G
Cuts at: 559 4054 4351 5266 5758 6020 559
Size: 3495 297 915 492 262 1735
Fragments arranged by size:
3495 1735 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7196
SphI G_CATG'C
Cuts at: 433 4249 4749 433
Size: 3816 500 2880
Fragments arranged by size:
3816 2880 500
SspI AAT'ATT
Cuts at: 1036 1036
Size: 7196
StuI AGG'CCT
Cuts at: 2350 2350
Size: 7196
StyI C'CwwG_G
Cuts at: 107 894 910 1884 2357 3870 3925 4012 4330
Size: 787 16 974 473 1513 55 87 318
Cuts at: 4330 4684 107
Size: 354 2619
Fragments arranged by size:
2619 1513 974 787 473 354 318 87
55 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 200 629 1172 1842 3071 3092 3235 4101 4779
Size: 429 543 670 1229 21 143 866 678
Cuts at: 4779 4912 5371 5580 200
Size: 133 459 209 1816
Fragments arranged by size:
1816 1229 866 678 670 543 459 429
209 143 133 21
TatI w'GTAC_w
Cuts at: 530 1395 1435 2237 3119 6711 530
Size: 865 40 802 882 3592 1015
Fragments arranged by size:
3592 1015 882 865 802 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1284 1316 6713 6745 1284
Size: 32 5397 32 1735
Fragments arranged by size:
5397 1735 32 32
Tth111I GACn'n_nGTC
Cuts at: 4993 4993
Size: 7196
XbaI T'CTAG_A
Cuts at: 4227 4227
Size: 7196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 198 1024 1380 1891 2127 3975 4691 198
Size: 826 356 511 236 1848 716 2703
Fragments arranged by size:
2703 1848 826 716 511 356 236
XmaI C'CCGG_G
Cuts at: 998 2432 4216 998
Size: 1434 1784 3978
Fragments arranged by size:
3978 1784 1434
XmnI GAAnn'nnTTC
Cuts at: 500 3172 3790 500
Size: 2672 618 3906
Fragments arranged by size:
3906 2672 618
ZraI GAC'GTC
Cuts at: 2988 2988
Size: 7196
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AscI AvrII BfrBI BmtI
BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI MfeI NheI NotI NruI NsiI PacI
PmlI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XhoI