Restriction map for
pF1KE2735
(Circular) (Six-base) MAPSORT of: pF1KE2735.seq Check: 3540 from: 1 to: 3869
pF1KE2735 3869 bp
With 182 enzymes: SgfI *
October 23, 2018 17:35 ..
AatII G_ACGT'C
Cuts at: 794 794
Size: 3869
AccI GT'mk_AC
Cuts at: 907 907
Size: 3869
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 1694 2126 2873 3141 115
Size: 1579 432 747 268 843
Fragments arranged by size:
1579 843 747 432 268
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 2257 2257
Size: 3869
AlwNI CAG_nnn'CTG
Cuts at: 138 1174 2741 138
Size: 1036 1567 1266
Fragments arranged by size:
1567 1266 1036
ApaI G_GGCC'C
Cuts at: 650 650
Size: 3869
ApaLI G'TGCA_C
Cuts at: 2639 2639
Size: 3869
ApoI r'AATT_y
Cuts at: 586 873 3222 3478 586
Size: 287 2349 256 977
Fragments arranged by size:
2349 977 287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 889 889
Size: 3869
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 885 1299 1547 1582 885
Size: 414 248 35 3172
Fragments arranged by size:
3172 414 248 35
BanII G_rGCy'C
Cuts at: 650 737 883 1913 650
Size: 87 146 1030 2606
Fragments arranged by size:
2606 1030 146 87
BbeI G_GCGC'C
Cuts at: 1303 1551 1303
Size: 248 3621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 770 736
Size: 34 3835
BciVI GTATCCnnnnn_n'
Cuts at: 1767 2528 3869 1767
Size: 761 1341 1767
Fragments arranged by size:
1767 1341 761
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglII A'GATC_T
Cuts at: 1384 3421 1384
Size: 2037 1832
BlpI GC'TnA_GC
Cuts at: 981 981
Size: 3869
Bme1580I G_kGCm'C
Cuts at: 650 759 1494 1587 2643 650
Size: 109 735 93 1056 1876
Fragments arranged by size:
1876 1056 735 109 93
BmgBI CAC'GTC
Cuts at: 569 569
Size: 3869
BmrI ACTGGGnnnn_n'
Cuts at: 471 1081 1162 1497 3641 471
Size: 610 81 335 2144 699
Fragments arranged by size:
2144 699 610 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 337 406 562 717 336
Size: 1 69 156 155 3488
Fragments arranged by size:
3488 156 155 69 1
Bpu10I CC'TnA_GC
Cuts at: 400 400
Size: 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 282 1045 1924 2416 2714 282
Size: 763 879 492 298 1437
Fragments arranged by size:
1437 879 763 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3573 3573
Size: 3869
BsaAI yAC'GTr
Cuts at: 369 1222 369
Size: 853 3016
BsaBI GATnn'nnATC
Cuts at: 6 1383 3420 6
Size: 1377 2037 455
Fragments arranged by size:
2037 1377 455
BsaHI Gr'CG_yC
Cuts at: 521 791 1300 1548 2250 3746 521
Size: 270 509 248 702 1496 644
Fragments arranged by size:
1496 702 644 509 270 248
BsaWI w'CCGG_w
Cuts at: 1058 1579 2257 2531 2678 3089 1058
Size: 521 678 274 147 411 1838
Fragments arranged by size:
1838 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 266 296 266
Size: 30 3839
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 208 592 755 163
Size: 45 384 163 3277
Fragments arranged by size:
3277 384 163 45
BseYI C'CCAG_C
Cuts at: 182 311 812 1294 2052 2629 3125 182
Size: 129 501 482 758 577 496 926
Fragments arranged by size:
926 758 577 501 496 482 129
BsiEI CG_ry'CG
Cuts at: 104 777 1411 1457 2665 104
Size: 673 634 46 1208 1308
Fragments arranged by size:
1308 1208 673 634 46
BsiHKAI G_wGCw'C
Cuts at: 241 268 473 883 1661 1851 2643 241
Size: 27 205 410 778 190 792 1467
Fragments arranged by size:
1467 792 778 410 205 190 27
BsmBI CGTCTCn'nnnn_
Cuts at: 208 673 208
Size: 465 3404
Bsp1286I G_dGCh'C
Cuts at: 241 268 473 650 737 759 883 1494 1587
Size: 27 205 177 87 22 124 611 93
Cuts at: 1587 1661 1851 1913 2643 241
Size: 74 190 62 730 1467
Fragments arranged by size:
1467 730 611 205 190 177 124 93
87 74 62 27 22
BspEI T'CCGG_A
Cuts at: 3089 3089
Size: 3869
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 398 454 919 1133 1435 1816 398
Size: 56 465 214 302 381 2451
Fragments arranged by size:
2451 465 381 302 214 56
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 285 435 1781 285
Size: 150 1346 2373
Fragments arranged by size:
2373 1346 150
BsrFI r'CCGG_y
Cuts at: 536 1867 2048 2257 536
Size: 1331 181 209 2148
Fragments arranged by size:
2148 1331 209 181
BssSI C'ACGA_G
Cuts at: 472 2140 2498 472
Size: 1668 358 1843
Fragments arranged by size:
1843 1668 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1246
Size: 3869
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstXI CCAn_nnnn'nTGG
Cuts at: 430 430
Size: 3869
BstYI r'GATC_y
Cuts at: 1 894 1361 1384 1719 1965 2977 3063 3075
Size: 893 467 23 335 246 1012 86 12
Cuts at: 3075 3421 1
Size: 346 449
Fragments arranged by size:
1012 893 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 729 729
Size: 3869
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1819 2000 2163 1819
Size: 181 163 3525
Fragments arranged by size:
3525 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1112
Size: 3869
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DraIII CAC_nnn'GTG
Cuts at: 558 558
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 726 774 1454 1628 2019 2046 3391 726
Size: 48 680 174 391 27 1345 1204
Fragments arranged by size:
1345 1204 680 391 174 48 27
EagI C'GGCC_G
Cuts at: 774 1454 774
Size: 680 3189
EarI CTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2077 2387 2533 3679 2077
Size: 310 146 1146 2267
Fragments arranged by size:
2267 1146 310 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 336 337 406 562 717 1694 2126 2873
Size: 221 1 69 156 155 977 432 747
Cuts at: 2873 3141 115
Size: 268 843
Fragments arranged by size:
977 843 747 432 268 221 156 155
69 1
EcoO109I rG'GnC_Cy
Cuts at: 204 333 443 607 647 803 1008 204
Size: 129 110 164 40 156 205 3065
Fragments arranged by size:
3065 205 164 156 129 110 40
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3869
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3869
HaeII r_GCGC'y
Cuts at: 421 1303 1551 2573 3552 421
Size: 882 248 1022 979 738
Fragments arranged by size:
1022 979 882 738 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 330 362 1711 1741 1743 1773 330
Size: 32 1349 30 2 30 2426
Fragments arranged by size:
2426 1349 32 30 30 2
HincII GTy'rAC
Cuts at: 459 908 459
Size: 449 3420
KasI G'GCGC_C
Cuts at: 1299 1547 1299
Size: 248 3621
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2540 2724 2540
Size: 184 3685
MscI TGG'CCA
Cuts at: 1630 1630
Size: 3869
MslI CAynn'nnrTG
Cuts at: 622 1858 1985 622
Size: 1236 127 2506
Fragments arranged by size:
2506 1236 127
MspA1I CmG'CkG
Cuts at: 186 315 482 779 812 980 1294 1654 2667
Size: 129 167 297 33 168 314 360 1013
Cuts at: 2667 2912 186
Size: 245 1143
Fragments arranged by size:
1143 1013 360 314 297 245 168 167
129 33
NaeI GCC'GGC
Cuts at: 538 2050 538
Size: 1512 2357
NarI GG'CG_CC
Cuts at: 1300 1548 1300
Size: 248 3621
NgoMIV G'CCGG_C
Cuts at: 536 2048 536
Size: 1512 2357
NspI r_CATG'y
Cuts at: 922 1422 2329 922
Size: 500 907 2462
Fragments arranged by size:
2462 907 500
PasI CC'CwG_GG
Cuts at: 270 270
Size: 3869
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PfoI T'CCnGG_A
Cuts at: 502 502
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PmlI CAC'GTG
Cuts at: 369 369
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PpuMI rG'GwC_Cy
Cuts at: 333 443 333
Size: 110 3759
PspOMI G'GGCC_C
Cuts at: 646 646
Size: 3869
PstI C_TGCA'G
Cuts at: 191 916 1601 191
Size: 725 685 2459
Fragments arranged by size:
2459 725 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 186 315 482 1294 1654 186
Size: 129 167 812 360 2401
Fragments arranged by size:
2401 812 360 167 129
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SexAI A'CCwGG_T
Cuts at: 554 554
Size: 3869
SfcI C'TryA_G
Cuts at: 33 187 912 1597 2590 2781 33
Size: 154 725 685 993 191 1121
Fragments arranged by size:
1121 993 725 685 191 154
SfoI GGC'GCC
Cuts at: 1301 1549 1301
Size: 248 3621
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 891 891
Size: 3869
SmlI C'TyrA_G
Cuts at: 261 1024 1939 2431 2693 261
Size: 763 915 492 262 1437
Fragments arranged by size:
1437 915 763 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3369
StuI AGG'CCT
Cuts at: 511 511
Size: 3869
StyI C'CwwG_G
Cuts at: 113 1003 1357 113
Size: 890 354 2625
Fragments arranged by size:
2625 890 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 453 648 1452 1585 2044 2253 283
Size: 170 195 804 133 459 209 1899
Fragments arranged by size:
1899 804 459 209 195 170 133
TatI w'GTAC_w
Cuts at: 3384 3384
Size: 3869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 3386 3418 266
Size: 32 3088 32 717
Fragments arranged by size:
3088 717 32 32
Tth111I GACn'n_nGTC
Cuts at: 790 1666 790
Size: 876 2993
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1364 1364
Size: 3869
XmaI C'CCGG_G
Cuts at: 889 889
Size: 3869
XmnI GAAnn'nnTTC
Cuts at: 376 376
Size: 3869
ZraI GAC'GTC
Cuts at: 792 792
Size: 3869
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI