Restriction map for
pF1KE2730
(Circular) (Six-base) MAPSORT of: pF1KE2730.seq Check: 2728 from: 1 to: 3770
pF1KE2730 3770 bp
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 247 247
Size: 3770
AccI GT'mk_AC
Cuts at: 808 808
Size: 3770
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 1595 2027 2774 3042 572
Size: 1023 432 747 268 1300
Fragments arranged by size:
1300 1023 747 432 268
AflIII A'CryG_T
Cuts at: 1122 2226 3203 1122
Size: 1104 977 1689
Fragments arranged by size:
1689 1104 977
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 1075 2642 1075
Size: 1567 2203
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 281 774 3123 3379 281
Size: 493 2349 256 672
Fragments arranged by size:
2349 672 493 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 790 790
Size: 3770
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 177 235 786 1200 1448 1483 177
Size: 58 551 414 248 35 2464
Fragments arranged by size:
2464 551 414 248 58 35
BanII G_rGCy'C
Cuts at: 743 784 1814 743
Size: 41 1030 2699
Fragments arranged by size:
2699 1030 41
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 149 1290 149
Size: 1141 2629
BfrBI ATG'CAT
Cuts at: 711 711
Size: 3770
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 882 882
Size: 3770
Bme1580I G_kGCm'C
Cuts at: 503 1395 1488 2544 503
Size: 892 93 1056 1729
Fragments arranged by size:
1729 1056 892 93
BmrI ACTGGGnnnn_n'
Cuts at: 982 1063 1398 3542 982
Size: 81 335 2144 1210
Fragments arranged by size:
2144 1210 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 586 471
Size: 115 3655
Bpu10I CC'TnA_GC
Cuts at: 433 433
Size: 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 946 1825 2317 2615 727
Size: 219 879 492 298 1882
Fragments arranged by size:
1882 879 492 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 169 1123 169
Size: 954 2816
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 1201 1449 2151 3647 1201
Size: 248 702 1496 1324
Fragments arranged by size:
1496 1324 702 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 671 671
Size: 3770
BseYI C'CCAG_C
Cuts at: 1195 1953 2530 3026 1195
Size: 758 577 496 1939
Fragments arranged by size:
1939 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 531 531
Size: 3770
BsiEI CG_ry'CG
Cuts at: 104 1312 1358 2566 104
Size: 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 46
BsiHKAI G_wGCw'C
Cuts at: 743 784 1562 1752 2544 743
Size: 41 778 190 792 1969
Fragments arranged by size:
1969 792 778 190 41
Bsp1286I G_dGCh'C
Cuts at: 503 743 784 1395 1488 1562 1752 1814 2544
Size: 240 41 611 93 74 190 62 730
Cuts at: 2544 503
Size: 1729
Fragments arranged by size:
1729 730 611 240 190 93 74 62
41
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 2946 2946
Size: 3770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 247 820 1034 1336 1717 247
Size: 573 214 302 381 2300
Fragments arranged by size:
2300 573 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2062 2116 3600 3765 50
Size: 2012 54 1484 165 55
Fragments arranged by size:
2012 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1682 1682
Size: 3770
BsrFI r'CCGG_y
Cuts at: 1768 1949 2158 1768
Size: 181 209 3380
Fragments arranged by size:
3380 209 181
BssSI C'ACGA_G
Cuts at: 653 2041 2399 653
Size: 1388 358 2024
Fragments arranged by size:
2024 1388 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1147 1147
Size: 3770
BstBI TT'CG_AA
Cuts at: 252 644 2131 252
Size: 392 1487 1891
Fragments arranged by size:
1891 1487 392
BstYI r'GATC_y
Cuts at: 1 795 1262 1285 1620 1866 2878 2964 2976
Size: 794 467 23 335 246 1012 86 12
Cuts at: 2976 3322 1
Size: 346 449
Fragments arranged by size:
1012 794 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3770
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 621 1720 1901 2064 621
Size: 1099 181 163 2327
Fragments arranged by size:
2327 1099 181 163
BtsI GCAGTG_nn'
Cuts at: 1013 1013
Size: 3770
DraI TTT'AAA
Cuts at: 534 769 534
Size: 235 3535
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 1355 1529 1920 1947 3292 1355
Size: 174 391 27 1345 1833
Fragments arranged by size:
1833 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1355 1355
Size: 3770
EarI CTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 2288 2434 3580 1978
Size: 310 146 1146 2168
Fragments arranged by size:
2168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 741 782 741
Size: 41 3729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 572 586 1595 2027 2774 3042 471
Size: 101 14 1009 432 747 268 1199
Fragments arranged by size:
1199 1009 747 432 268 101 14
EcoO109I rG'GnC_Cy
Cuts at: 909 909
Size: 3770
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 1204 1452 2474 3453 1204
Size: 248 1022 979 1521
Fragments arranged by size:
1521 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1612 1642 1644 1674 1612
Size: 30 2 30 3708
Fragments arranged by size:
3708 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2625 2441
Size: 184 3586
MscI TGG'CCA
Cuts at: 1531 1531
Size: 3770
MslI CAynn'nnrTG
Cuts at: 588 1759 1886 588
Size: 1171 127 2472
Fragments arranged by size:
2472 1171 127
MspA1I CmG'CkG
Cuts at: 226 564 881 1195 1555 2568 2813 226
Size: 338 317 314 360 1013 245 1183
Fragments arranged by size:
1183 1013 360 338 317 314 245
NaeI GCC'GGC
Cuts at: 1951 1951
Size: 3770
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NcoI C'CATG_G
Cuts at: 107 107
Size: 3770
NgoMIV G'CCGG_C
Cuts at: 1949 1949
Size: 3770
NsiI A_TGCA'T
Cuts at: 713 713
Size: 3770
NspI r_CATG'y
Cuts at: 432 544 711 823 1323 2230 432
Size: 112 167 112 500 907 1972
Fragments arranged by size:
1972 907 500 167 112 112
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PfoI T'CCnGG_A
Cuts at: 487 487
Size: 3770
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 715 747 2909 2941 715
Size: 32 2162 32 1544
Fragments arranged by size:
2162 1544 32 32
PstI C_TGCA'G
Cuts at: 817 1502 817
Size: 685 3085
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 226 564 1195 1555 226
Size: 338 631 360 2441
Fragments arranged by size:
2441 631 360 338
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 743 784 743
Size: 41 3729
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SexAI A'CCwGG_T
Cuts at: 572 572
Size: 3770
SfcI C'TryA_G
Cuts at: 33 813 1498 2491 2682 33
Size: 780 685 993 191 1121
Fragments arranged by size:
1121 993 780 685 191
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 742 925 1840 2332 2594 742
Size: 183 915 492 262 1918
Fragments arranged by size:
1918 915 492 262 183
SnaBI TAC'GTA
Cuts at: 169 169
Size: 3770
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 544 823 1323 544
Size: 279 500 2991
Fragments arranged by size:
2991 500 279
StyI C'CwwG_G
Cuts at: 107 904 1258 107
Size: 797 354 2619
Fragments arranged by size:
2619 797 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1353 1486 1945 2154 1353
Size: 133 459 209 2969
Fragments arranged by size:
2969 459 209 133
TatI w'GTAC_w
Cuts at: 3285 3285
Size: 3770
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 3738
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1265 1265
Size: 3770
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
XmnI GAAnn'nnTTC
Cuts at: 281 281
Size: 3770
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI