Restriction map for
pF1KE2729
(Circular) (Six-base) MAPSORT of: pF1KE2729.seq Check: 467 from: 1 to: 4508
pF1KE2729 4508 bp
With 182 enzymes: SgfI *
October 2, 2018 18:08 ..
AccI GT'mk_AC
Cuts at: 1546 1546
Size: 4508
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2765 3512 3780 2333
Size: 432 747 268 3061
Fragments arranged by size:
3061 747 432 268
AfeI AGC'GCT
Cuts at: 949 949
Size: 4508
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AlwNI CAG_nnn'CTG
Cuts at: 1813 3380 1813
Size: 1567 2941
ApaI G_GGCC'C
Cuts at: 1455 1455
Size: 4508
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 1512 3861 4117 1512
Size: 2349 256 1903
Fragments arranged by size:
2349 1903 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 1528 1528
Size: 4508
AvrII C'CTAG_G
Cuts at: 621 621
Size: 4508
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 825 858 825
Size: 33 4475
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 301 639 1524 1938 2186 2221 301
Size: 338 885 414 248 35 2588
Fragments arranged by size:
2588 885 414 338 248 35
BanII G_rGCy'C
Cuts at: 653 679 1455 1489 1522 2552 653
Size: 26 776 34 33 1030 2609
Fragments arranged by size:
2609 1030 776 34 33 26
BbeI G_GCGC'C
Cuts at: 643 1942 2190 643
Size: 1299 248 2961
Fragments arranged by size:
2961 1299 248
BbsI GAAGACnn'nnnn_
Cuts at: 909 1484 909
Size: 575 3933
BbvCI CC'TCA_GC
Cuts at: 696 1316 696
Size: 620 3888
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglI GCCn_nnn'nGGC
Cuts at: 648 648
Size: 4508
BglII A'GATC_T
Cuts at: 2023 4060 2023
Size: 2037 2471
BlpI GC'TnA_GC
Cuts at: 1620 1620
Size: 4508
Bme1580I G_kGCm'C
Cuts at: 365 1131 1455 2133 2226 3282 365
Size: 766 324 678 93 1056 1591
Fragments arranged by size:
1591 1056 766 678 324 93
BmrI ACTGGGnnnn_n'
Cuts at: 1134 1720 1801 2136 4280 1134
Size: 586 81 335 2144 1362
Fragments arranged by size:
2144 1362 586 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 663 755 770 1122 1226 1258 648
Size: 15 92 15 352 104 32 3898
Fragments arranged by size:
3898 352 104 92 32 15 15
Bpu10I CC'TnA_GC
Cuts at: 696 1316 696
Size: 620 3888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2563 3055 3353 1684
Size: 879 492 298 2839
Fragments arranged by size:
2839 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4212 4212
Size: 4508
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 640 1939 2187 2889 4385 640
Size: 1299 248 702 1496 763
Fragments arranged by size:
1496 1299 763 702 248
BsaWI w'CCGG_w
Cuts at: 1697 2218 2896 3170 3317 3728 1697
Size: 521 678 274 147 411 2477
Fragments arranged by size:
2477 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 385 415 1144 1174 385
Size: 30 729 30 3719
Fragments arranged by size:
3719 729 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 1387 201
Size: 1186 3322
BseYI C'CCAG_C
Cuts at: 712 1037 1933 2691 3268 3764 712
Size: 325 896 758 577 496 1456
Fragments arranged by size:
1456 896 758 577 496 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1249
Size: 4508
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3304 104
Size: 1946 46 1208 1308
Fragments arranged by size:
1946 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1522 2300 2490 3282 1522
Size: 778 190 792 2748
Fragments arranged by size:
2748 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 388 1440 388
Size: 1052 3456
Bsp1286I G_dGCh'C
Cuts at: 365 653 679 1131 1455 1489 1522 2133 2226
Size: 288 26 452 324 34 33 611 93
Cuts at: 2226 2300 2490 2552 3282 365
Size: 74 190 62 730 1591
Fragments arranged by size:
1591 730 611 452 324 288 190 93
74 62 34 33 26
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 4508
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1558 1772 2074 2455 1558
Size: 214 302 381 3611
Fragments arranged by size:
3611 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2800 2854 4338 4503 50
Size: 2750 54 1484 165 55
Fragments arranged by size:
2750 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 725 2420 725
Size: 1695 2813
BsrFI r'CCGG_y
Cuts at: 2506 2687 2896 2506
Size: 181 209 4118
Fragments arranged by size:
4118 209 181
BsrGI T'GTAC_A
Cuts at: 248 248
Size: 4508
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 1064 1885 1064
Size: 821 3687
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 555 712 1170 555
Size: 157 458 3893
Fragments arranged by size:
3893 458 157
BstYI r'GATC_y
Cuts at: 1 1533 2000 2023 2358 2604 3616 3702 3714
Size: 1532 467 23 335 246 1012 86 12
Cuts at: 3714 4060 1
Size: 346 449
Fragments arranged by size:
1532 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 902 902
Size: 4508
BtgI C'CryG_G
Cuts at: 107 1222 1470 107
Size: 1115 248 3145
Fragments arranged by size:
3145 1115 248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 153 1308 2458 2639 2802 153
Size: 1155 1150 181 163 1859
Fragments arranged by size:
1859 1155 1150 181 163
BtsI GCAGTG_nn'
Cuts at: 653 822 1017 1751 653
Size: 169 195 734 3410
Fragments arranged by size:
3410 734 195 169
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 2093 2267 2658 2685 4030 2093
Size: 174 391 27 1345 2571
Fragments arranged by size:
2571 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 1312 1349 2531 2741 1312
Size: 37 1182 210 3079
Fragments arranged by size:
3079 1182 210 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2716 3026 3172 4318 2716
Size: 310 146 1146 2906
Fragments arranged by size:
2906 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1520 1520
Size: 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 663 755 770 1122 1226 1258 2333 2765
Size: 15 92 15 352 104 32 1075 432
Cuts at: 2765 3512 3780 648
Size: 747 268 1376
Fragments arranged by size:
1376 1075 747 432 352 268 104 92
32 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1459 1459
Size: 4508
EcoO109I rG'GnC_Cy
Cuts at: 1452 1647 1452
Size: 195 4313
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4508
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 643 951 1202 1942 2190 3212 4191 643
Size: 308 251 740 248 1022 979 960
Fragments arranged by size:
1022 979 960 740 308 251 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 387 419 485 517 592 624 1144 1176 2350
Size: 32 66 32 75 32 520 32 1174
Cuts at: 2350 2380 2382 2412 387
Size: 30 2 30 2483
Fragments arranged by size:
2483 1174 520 75 66 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1547 1547
Size: 4508
HindIII A'AGCT_T
Cuts at: 1411 1411
Size: 4508
KasI G'GCGC_C
Cuts at: 639 1938 2186 639
Size: 1299 248 2961
Fragments arranged by size:
2961 1299 248
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 349 3179 3363 107
Size: 242 2830 184 1252
Fragments arranged by size:
2830 1252 242 184
MscI TGG'CCA
Cuts at: 2269 2269
Size: 4508
MslI CAynn'nnrTG
Cuts at: 533 2497 2624 533
Size: 1964 127 2417
Fragments arranged by size:
2417 1964 127
MspA1I CmG'CkG
Cuts at: 1315 1619 1933 2293 3306 3551 1315
Size: 304 314 360 1013 245 2272
Fragments arranged by size:
2272 1013 360 314 304 245
NaeI GCC'GGC
Cuts at: 2689 2689
Size: 4508
NarI GG'CG_CC
Cuts at: 640 1939 2187 640
Size: 1299 248 2961
Fragments arranged by size:
2961 1299 248
NcoI C'CATG_G
Cuts at: 107 1222 107
Size: 1115 3393
NgoMIV G'CCGG_C
Cuts at: 2687 2687
Size: 4508
NspI r_CATG'y
Cuts at: 1561 2061 2968 1561
Size: 500 907 3101
Fragments arranged by size:
3101 907 500
PasI CC'CwG_GG
Cuts at: 672 672
Size: 4508
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PfoI T'CCnGG_A
Cuts at: 1091 1091
Size: 4508
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4476
PspOMI G'GGCC_C
Cuts at: 1451 1451
Size: 4508
PstI C_TGCA'G
Cuts at: 660 888 1060 1217 1555 2240 660
Size: 228 172 157 338 685 2928
Fragments arranged by size:
2928 685 338 228 172 157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 1315 1933 2293 1315
Size: 618 360 3530
Fragments arranged by size:
3530 618 360
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1522 1522
Size: 4508
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 1312 2531 2741 1312
Size: 1219 210 3079
Fragments arranged by size:
3079 1219 210
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 137 313 656 719 884 1056 1213 1551
Size: 104 176 343 63 165 172 157 338
Cuts at: 1551 2236 3229 3420 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 343 338 191 176 172
165 157 104 63
SfoI GGC'GCC
Cuts at: 641 1940 2188 641
Size: 1299 248 2961
Fragments arranged by size:
2961 1299 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 1530 1530
Size: 4508
SmlI C'TyrA_G
Cuts at: 1663 2578 3070 3332 1663
Size: 915 492 262 2839
Fragments arranged by size:
2839 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
StyI C'CwwG_G
Cuts at: 107 621 1222 1642 1996 107
Size: 514 601 420 354 2619
Fragments arranged by size:
2619 601 514 420 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1215 1235 2091 2224 2683 2892 1215
Size: 20 856 133 459 209 2831
Fragments arranged by size:
2831 856 459 209 133 20
TatI w'GTAC_w
Cuts at: 248 4023 248
Size: 3775 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4025 4057 4025
Size: 32 4476
Tth111I GACn'n_nGTC
Cuts at: 604 2305 604
Size: 1701 2807
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1468 2003 1468
Size: 535 3973
XmaI C'CCGG_G
Cuts at: 1528 1528
Size: 4508
XmnI GAAnn'nnTTC
Cuts at: 161 161
Size: 4508
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI