Restriction map for
pF1KE2727
(Circular) (Six-base) MAPSORT of: pF1KE2727.seq Check: 2923 from: 1 to: 6755
pF1KE2727 6755 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2734 3145 2734
Size: 411 6344
AccI GT'mk_AC
Cuts at: 3793 3793
Size: 6755
Acc65I G'GTAC_C
Cuts at: 737 1661 3771 737
Size: 924 2110 3721
Fragments arranged by size:
3721 2110 924
AclI AA'CG_TT
Cuts at: 6595 6595
Size: 6755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2220 3571 4580 5012 5759 6027 2220
Size: 1351 1009 432 747 268 2948
Fragments arranged by size:
2948 1351 1009 747 432 268
AflIII A'CryG_T
Cuts at: 2894 4107 5211 6188 2894
Size: 1213 1104 977 3461
Fragments arranged by size:
3461 1213 1104 977
AgeI A'CCGG_T
Cuts at: 740 5143 740
Size: 4403 2352
AhdI GACnn_n'nnGTC
Cuts at: 1545 1545
Size: 6755
AleI CACnn'nnGTG
Cuts at: 652 2142 652
Size: 1490 5265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1738 1738
Size: 6755
AlwNI CAG_nnn'CTG
Cuts at: 516 2130 2604 2673 3083 3621 4060 5627 516
Size: 1614 474 69 410 538 439 1567 1644
Fragments arranged by size:
1644 1614 1567 538 474 439 410 69
ApaI G_GGCC'C
Cuts at: 490 2184 2641 2861 3126 490
Size: 1694 457 220 265 4119
Fragments arranged by size:
4119 1694 457 265 220
ApaLI G'TGCA_C
Cuts at: 5525 5525
Size: 6755
ApoI r'AATT_y
Cuts at: 1222 3310 3759 6108 6364 1222
Size: 2088 449 2349 256 1613
Fragments arranged by size:
2349 2088 1613 449 256
AscI GG'CGCG_CC
Cuts at: 2438 2438
Size: 6755
AseI AT'TA_AT
Cuts at: 20 2297 20
Size: 2277 4478
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6755
AvaI C'yCGr_G
Cuts at: 1100 1768 2641 2760 3424 3775 1100
Size: 668 873 119 664 351 4080
Fragments arranged by size:
4080 873 668 664 351 119
BamHI G'GATC_C
Cuts at: 137 399 3780 137
Size: 262 3381 3112
Fragments arranged by size:
3381 3112 262
BanI G'GyrC_C
Cuts at: 114 221 646 737 1506 1561 1661 1675 2402
Size: 107 425 91 769 55 100 14 727
Cuts at: 2402 2408 2529 3133 3771 4185 4433 4468 114
Size: 6 121 604 638 414 248 35 2401
Fragments arranged by size:
2401 769 727 638 604 425 414 248
121 107 100 91 55 35 14 6
BanII G_rGCy'C
Cuts at: 490 724 1187 1270 1601 2184 2258 2641 2861
Size: 234 463 83 331 583 74 383 220
Cuts at: 2861 2960 3098 3126 3403 3769 4799 490
Size: 99 138 28 277 366 1030 2446
Fragments arranged by size:
2446 1030 583 463 383 366 331 277
234 220 138 99 83 74 28
BbeI G_GCGC'C
Cuts at: 225 1510 1679 2406 2533 4189 4437 225
Size: 1285 169 727 127 1656 248 2543
Fragments arranged by size:
2543 1656 1285 727 248 169 127
BbsI GAAGACnn'nnnn_
Cuts at: 166 895 1061 2344 2997 166
Size: 729 166 1283 653 3924
Fragments arranged by size:
3924 1283 729 653 166
BbvCI CC'TCA_GC
Cuts at: 1278 1453 1627 1869 1925 1278
Size: 175 174 242 56 6108
Fragments arranged by size:
6108 242 175 174 56
BciVI GTATCCnnnnn_n'
Cuts at: 3001 4653 5414 6755 3001
Size: 1652 761 1341 3001
Fragments arranged by size:
3001 1652 1341 761
BclI T'GATC_A
Cuts at: 4275 4275
Size: 6755
BglI GCCn_nnn'nGGC
Cuts at: 268 839 985 2366 268
Size: 571 146 1381 4657
Fragments arranged by size:
4657 1381 571 146
BglII A'GATC_T
Cuts at: 146 3026 4270 6307 146
Size: 2880 1244 2037 594
Fragments arranged by size:
2880 2037 1244 594
BlpI GC'TnA_GC
Cuts at: 989 3867 989
Size: 2878 3877
Bme1580I G_kGCm'C
Cuts at: 450 490 649 1806 1824 2184 2641 2861 3111
Size: 40 159 1157 18 360 457 220 250
Cuts at: 3111 3126 3136 4380 4473 5529 450
Size: 15 10 1244 93 1056 1676
Fragments arranged by size:
1676 1244 1157 1056 457 360 250 220
159 93 40 18 15 10
BmgBI CAC'GTC
Cuts at: 1614 2895 3060 3560 1614
Size: 1281 165 500 4809
Fragments arranged by size:
4809 1281 500 165
BmrI ACTGGGnnnn_n'
Cuts at: 581 650 3141 3967 4048 4383 6527 581
Size: 69 2491 826 81 335 2144 809
Fragments arranged by size:
2491 2144 826 809 335 81 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 721 3555 5122 721
Size: 2834 1567 2354
Fragments arranged by size:
2834 2354 1567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 886 1803 1933 2056 2566 3652 481
Size: 405 917 130 123 510 1086 3584
Fragments arranged by size:
3584 1086 917 510 405 130 123
Bpu10I CC'TnA_GC
Cuts at: 1017 1278 1453 1627 1869 1925 2605 3373 3685
Size: 261 175 174 242 56 680 768 312
Cuts at: 3685 1017
Size: 4087
Fragments arranged by size:
4087 768 680 312 261 242 175 174
56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 3931 4810 5302 5600 1159
Size: 2772 879 492 298 2314
Fragments arranged by size:
2772 2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1039 1222 6459 1039
Size: 183 5237 1335
Fragments arranged by size:
5237 1335 183
BsaAI yAC'GTr
Cuts at: 4108 4108
Size: 6755
BsaBI GATnn'nnATC
Cuts at: 6 2869 4269 6306 6
Size: 2863 1400 2037 455
Fragments arranged by size:
2863 2037 1400 455
BsaHI Gr'CG_yC
Cuts at: 222 1400 1507 1546 1676 2093 2341 2403 2530
Size: 1178 107 39 130 417 248 62 127
Cuts at: 2530 4186 4434 5136 6632 222
Size: 1656 248 702 1496 345
Fragments arranged by size:
1656 1496 1178 702 417 345 248 248
130 127 107 62 39
BsaWI w'CCGG_w
Cuts at: 689 740 3944 4465 5143 5417 5564 5975 689
Size: 51 3204 521 678 274 147 411 1469
Fragments arranged by size:
3204 1469 678 521 411 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1702 1732 2260 2290 1702
Size: 30 528 30 6167
Fragments arranged by size:
6167 528 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 922 994 1942 2020 2023 2266 2281 3166 922
Size: 72 948 78 3 243 15 885 4511
Fragments arranged by size:
4511 948 885 243 78 72 15 3
BseYI C'CCAG_C
Cuts at: 471 1572 1638 2328 2346 2853 3404 3591 4180
Size: 1101 66 690 18 507 551 187 589
Cuts at: 4180 4938 5515 6011 471
Size: 758 577 496 1215
Fragments arranged by size:
1215 1101 758 690 589 577 551 507
496 187 66 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 565 3405 150
Size: 415 2840 3500
Fragments arranged by size:
3500 2840 415
BsiEI CG_ry'CG
Cuts at: 104 360 597 4297 4343 5551 104
Size: 256 237 3700 46 1208 1308
Fragments arranged by size:
3700 1308 1208 256 237 46
BsiHKAI G_wGCw'C
Cuts at: 553 1187 1270 1601 3077 3769 4547 4737 5529
Size: 634 83 331 1476 692 778 190 792
Cuts at: 5529 553
Size: 1779
Fragments arranged by size:
1779 1476 792 778 692 634 331 190
83
BsmBI CGTCTCn'nnnn_
Cuts at: 3147 3147
Size: 6755
Bsp1286I G_dGCh'C
Cuts at: 450 490 553 649 724 1187 1270 1601 1806
Size: 40 63 96 75 463 83 331 205
Cuts at: 1806 1824 2184 2258 2641 2861 2960 3077 3098
Size: 18 360 74 383 220 99 117 21
Cuts at: 3098 3111 3126 3136 3403 3769 4380 4473 4547
Size: 13 15 10 267 366 611 93 74
Cuts at: 4547 4737 4799 5529 450
Size: 190 62 730 1676
Fragments arranged by size:
1676 730 611 463 383 366 360 331
267 220 205 190 117 99 96 93
83 75 74 74 63 62 40 21
18 15 13 10
BspEI T'CCGG_A
Cuts at: 689 5975 689
Size: 5286 1469
BspHI T'CATG_A
Cuts at: 5931 5931
Size: 6755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 172 524 1799 2486 2734 3029 3145 3740 3805
Size: 352 1275 687 248 295 116 595 65
Cuts at: 3805 4019 4321 4702 172
Size: 214 302 381 2225
Fragments arranged by size:
2225 1275 687 595 381 352 302 295
248 214 116 65
BsrBI CCG'CTC
Cuts at: 50 1749 2049 2609 3462 3636 3692 5047 5101
Size: 1699 300 560 853 174 56 1355 54
Cuts at: 5101 6585 6750 50
Size: 1484 165 55
Fragments arranged by size:
1699 1484 1355 853 560 300 174 165
56 55 54
BsrDI GCAATG_nn'
Cuts at: 1328 3224 4667 1328
Size: 1896 1443 3416
Fragments arranged by size:
3416 1896 1443
BsrFI r'CCGG_y
Cuts at: 355 596 740 1415 2405 2442 2526 2750 4753
Size: 241 144 675 990 37 84 224 2003
Cuts at: 4753 4934 5143 355
Size: 181 209 1967
Fragments arranged by size:
2003 1967 990 675 241 224 209 181
144 84 37
BssHII G'CGCG_C
Cuts at: 1652 2438 1652
Size: 786 5969
BssSI C'ACGA_G
Cuts at: 1006 2512 3193 5026 5384 1006
Size: 1506 681 1833 358 2377
Fragments arranged by size:
2377 1833 1506 681 358
BstAPI GCAn_nnn'nTGC
Cuts at: 516 4132 516
Size: 3616 3139
BstBI TT'CG_AA
Cuts at: 5116 5116
Size: 6755
BstEII G'GTnAC_C
Cuts at: 279 751 2445 279
Size: 472 1694 4589
Fragments arranged by size:
4589 1694 472
BstXI CCAn_nnnn'nTGG
Cuts at: 657 669 2457 3162 657
Size: 12 1788 705 4250
Fragments arranged by size:
4250 1788 705 12
BstYI r'GATC_y
Cuts at: 1 137 146 192 399 3026 3780 4247 4270
Size: 136 9 46 207 2627 754 467 23
Cuts at: 4270 4605 4851 5863 5949 5961 6307 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2627 1012 754 467 449 346 335 246
207 136 86 46 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 2962 2962
Size: 6755
BtgI C'CryG_G
Cuts at: 107 251 275 308 662 1446 2948 2968 107
Size: 144 24 33 354 784 1502 20 3894
Fragments arranged by size:
3894 1502 784 354 144 33 24 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 311 4705 4886 5049 311
Size: 4394 181 163 2017
Fragments arranged by size:
4394 2017 181 163
BtsI GCAGTG_nn'
Cuts at: 251 2013 3655 3998 251
Size: 1762 1642 343 3008
Fragments arranged by size:
3008 1762 1642 343
DraI TTT'AAA
Cuts at: 3754 3754
Size: 6755
DrdI GACnn_nn'nnGTC
Cuts at: 922 1141 4461 5319 922
Size: 219 3320 858 2358
Fragments arranged by size:
3320 2358 858 219
EaeI y'GGCC_r
Cuts at: 248 357 594 632 659 1829 2078 2159 2649
Size: 109 237 38 27 1170 249 81 490
Cuts at: 2649 2945 3197 3674 4340 4514 4905 4932 6277
Size: 296 252 477 666 174 391 27 1345
Cuts at: 6277 248
Size: 726
Fragments arranged by size:
1345 1170 726 666 490 477 391 296
252 249 237 174 109 81 38 27
27
EagI C'GGCC_G
Cuts at: 357 594 4340 357
Size: 237 3746 2772
Fragments arranged by size:
3746 2772 237
EarI CTCTTCn'nnn_
Cuts at: 2243 2914 4778 4988 2243
Size: 671 1864 210 4010
Fragments arranged by size:
4010 1864 671 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1057 2338 4963 5273 5419 6565 1057
Size: 1281 2625 310 146 1146 1247
Fragments arranged by size:
2625 1281 1247 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1185 1268 1599 3767 1185
Size: 83 331 2168 4173
Fragments arranged by size:
4173 2168 331 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 886 1803 1933 2056 2220 2566 3571 3652
Size: 405 917 130 123 164 346 1005 81
Cuts at: 3652 4580 5012 5759 6027 481
Size: 928 432 747 268 1209
Fragments arranged by size:
1209 1005 928 917 747 432 405 346
268 164 130 123 81
EcoNI CCTnn'n_nnAGG
Cuts at: 1252 1691 1771 3448 1252
Size: 439 80 1677 4559
Fragments arranged by size:
4559 1677 439 80
EcoO109I rG'GnC_Cy
Cuts at: 203 486 487 513 1134 1143 1245 1683 1776
Size: 283 1 26 621 9 102 438 93
Cuts at: 1776 1880 2181 2308 2352 2601 2637 2638 2857
Size: 104 301 127 44 249 36 1 219
Cuts at: 2857 3122 3681 3894 203
Size: 265 559 213 3064
Fragments arranged by size:
3064 621 559 438 301 283 265 249
219 213 127 104 102 93 44 36
26 9 1 1
EcoRI G'AATT_C
Cuts at: 1222 3310 3759 1222
Size: 2088 449 4218
Fragments arranged by size:
4218 2088 449
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1153 3516 3591 4774 1153
Size: 2363 75 1183 3134
Fragments arranged by size:
3134 2363 1183 75
FseI GG_CCGG'CC
Cuts at: 359 359
Size: 6755
FspI TGC'GCA
Cuts at: 4536 4536
Size: 6755
HaeII r_GCGC'y
Cuts at: 225 1510 1679 2406 2533 2673 3621 4189 4437
Size: 1285 169 727 127 140 948 568 248
Cuts at: 4437 5459 6438 225
Size: 1022 979 542
Fragments arranged by size:
1285 1022 979 948 727 568 542 248
169 140 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1094 1126 1198 1230 1531 1563 1704 1736 4597
Size: 32 72 32 301 32 141 32 2861
Cuts at: 4597 4627 4629 4659 1094
Size: 30 2 30 3190
Fragments arranged by size:
3190 2861 301 141 72 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 183 962 1980 2844 3216 3794 183
Size: 779 1018 864 372 578 3144
Fragments arranged by size:
3144 1018 864 779 578 372
HindIII A'AGCT_T
Cuts at: 1385 1385
Size: 6755
KasI G'GCGC_C
Cuts at: 221 1506 1675 2402 2529 4185 4433 221
Size: 1285 169 727 127 1656 248 2543
Fragments arranged by size:
2543 1656 1285 727 248 169 127
KpnI G_GTAC'C
Cuts at: 741 1665 3775 741
Size: 924 2110 3721
Fragments arranged by size:
3721 2110 924
MfeI C'AATT_G
Cuts at: 3379 3379
Size: 6755
MluI A'CGCG_T
Cuts at: 6188 6188
Size: 6755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1538 3071 5426 5610 1538
Size: 1533 2355 184 2683
Fragments arranged by size:
2683 2355 1533 184
MscI TGG'CCA
Cuts at: 250 634 661 1831 2080 2161 2651 2947 3199
Size: 384 27 1170 249 81 490 296 252
Cuts at: 3199 4516 250
Size: 1317 2489
Fragments arranged by size:
2489 1317 1170 490 384 296 252 249
81 27
MslI CAynn'nnrTG
Cuts at: 112 432 605 652 667 2142 2455 3058 3229
Size: 320 173 47 15 1475 313 603 171
Cuts at: 3229 4744 4871 112
Size: 1515 127 1996
Fragments arranged by size:
1996 1515 1475 603 320 313 173 171
127 47 15
MspA1I CmG'CkG
Cuts at: 406 2076 2346 2393 2420 2633 2696 2853 3591
Size: 1670 270 47 27 213 63 157 738
Cuts at: 3591 3866 4180 4540 5553 5798 406
Size: 275 314 360 1013 245 1363
Fragments arranged by size:
1670 1363 1013 738 360 314 275 270
245 213 157 63 47 27
NaeI GCC'GGC
Cuts at: 357 598 2528 2752 4936 357
Size: 241 1930 224 2184 2176
Fragments arranged by size:
2184 2176 1930 241 224
NarI GG'CG_CC
Cuts at: 222 1507 1676 2403 2530 4186 4434 222
Size: 1285 169 727 127 1656 248 2543
Fragments arranged by size:
2543 1656 1285 727 248 169 127
NcoI C'CATG_G
Cuts at: 107 251 662 107
Size: 144 411 6200
Fragments arranged by size:
6200 411 144
NgoMIV G'CCGG_C
Cuts at: 355 596 2526 2750 4934 355
Size: 241 1930 224 2184 2176
Fragments arranged by size:
2184 2176 1930 241 224
NspI r_CATG'y
Cuts at: 604 1624 1719 3808 4308 5215 604
Size: 1020 95 2089 500 907 2144
Fragments arranged by size:
2144 2089 1020 907 500 95
PasI CC'CwG_GG
Cuts at: 677 1069 677
Size: 392 6363
PciI A'CATG_T
Cuts at: 5211 5211
Size: 6755
PflMI CCAn_nnn'nTGG
Cuts at: 630 1075 3570 630
Size: 445 2495 3815
Fragments arranged by size:
3815 2495 445
PfoI T'CCnGG_A
Cuts at: 195 3305 195
Size: 3110 3645
PmeI GTTT'AAAC
Cuts at: 3754 3754
Size: 6755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5894 5926 5894
Size: 32 6723
PpuMI rG'GwC_Cy
Cuts at: 203 513 1134 1143 1683 2352 203
Size: 310 621 9 540 669 4606
Fragments arranged by size:
4606 669 621 540 310 9
PspOMI G'GGCC_C
Cuts at: 486 2180 2637 2857 3122 486
Size: 1694 457 220 265 4119
Fragments arranged by size:
4119 1694 457 265 220
PspXI vC'TCGA_Gb
Cuts at: 3424 3424
Size: 6755
PstI C_TGCA'G
Cuts at: 411 512 1151 3026 3802 4487 411
Size: 101 639 1875 776 685 2679
Fragments arranged by size:
2679 1875 776 685 639 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6755
PvuII CAG'CTG
Cuts at: 406 2076 2696 2853 3591 4180 4540 406
Size: 1670 620 157 738 589 360 2621
Fragments arranged by size:
2621 1670 738 620 589 360 157
RsrII CG'GwC_CG
Cuts at: 230 4950 230
Size: 4720 2035
SacI G_AGCT'C
Cuts at: 1187 1270 1601 3769 1187
Size: 83 331 2168 4173
Fragments arranged by size:
4173 2168 331 83
SalI G'TCGA_C
Cuts at: 3792 3792
Size: 6755
SanDI GG'GwC_CC
Cuts at: 1683 2352 1683
Size: 669 6086
SapI GCTCTTCn'nnn_
Cuts at: 4778 4988 4778
Size: 210 6545
SbfI CC_TGCA'GG
Cuts at: 1151 3802 1151
Size: 2651 4104
ScaI AGT'ACT
Cuts at: 6272 6272
Size: 6755
SexAI A'CCwGG_T
Cuts at: 455 455
Size: 6755
SfcI C'TryA_G
Cuts at: 33 407 508 1147 1497 3022 3798 4483 5476
Size: 374 101 639 350 1525 776 685 993
Cuts at: 5476 5667 33
Size: 191 1121
Fragments arranged by size:
1525 1121 993 776 685 639 374 350
191 101
SfoI GGC'GCC
Cuts at: 223 1508 1677 2404 2531 4187 4435 223
Size: 1285 169 727 127 1656 248 2543
Fragments arranged by size:
2543 1656 1285 727 248 169 127
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6755
SgrAI Cr'CCGG_yG
Cuts at: 2405 2442 2405
Size: 37 6718
SmaI CCC'GGG
Cuts at: 2643 2762 3777 2643
Size: 119 1015 5621
Fragments arranged by size:
5621 1015 119
SmlI C'TyrA_G
Cuts at: 1138 3424 3910 4825 5317 5579 1138
Size: 2286 486 915 492 262 2314
Fragments arranged by size:
2314 2286 915 492 486 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6755
SphI G_CATG'C
Cuts at: 3808 4308 3808
Size: 500 6255
StuI AGG'CCT
Cuts at: 1258 1276 1258
Size: 18 6737
StyI C'CwwG_G
Cuts at: 107 251 650 662 716 3889 4243 107
Size: 144 399 12 54 3173 354 2619
Fragments arranged by size:
3173 2619 399 354 144 54 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 407 655 997 1706 2187 3660 4338 4471 4930
Size: 248 342 709 481 1473 678 133 459
Cuts at: 4930 5139 407
Size: 209 2023
Fragments arranged by size:
2023 1473 709 678 481 459 342 248
209 133
TatI w'GTAC_w
Cuts at: 1835 3255 6270 1835
Size: 1420 3015 2320
Fragments arranged by size:
3015 2320 1420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6272 6304 6272
Size: 32 6723
Tth111I GACn'n_nGTC
Cuts at: 766 4552 766
Size: 3786 2969
XbaI T'CTAG_A
Cuts at: 3786 3786
Size: 6755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2181 3162 3207 4250 2181
Size: 981 45 1043 4686
Fragments arranged by size:
4686 1043 981 45
XhoI C'TCGA_G
Cuts at: 3424 3424
Size: 6755
XmaI C'CCGG_G
Cuts at: 2641 2760 3775 2641
Size: 119 1015 5621
Fragments arranged by size:
5621 1015 119
XmnI GAAnn'nnTTC
Cuts at: 850 1346 850
Size: 496 6259
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsmI BsrGI BstZ17I ClaI CspCI DraIII
EcoRV FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PsrI SacII SfiI SnaBI
SrfI SspI SwaI ZraI