Restriction map for
pF1KE2719
(Circular) (Six-base) MAPSORT of: pF1KE2719.seq Check: 8259 from: 1 to: 10883
pF1KE2719 10883 bp
With 182 enzymes: SgfI *
February 25, 2018 13:08 ..
AccI GT'mk_AC
Cuts at: 611 1506 4689 7921 611
Size: 895 3183 3232 3573
Fragments arranged by size:
3573 3232 3183 895
Acc65I G'GTAC_C
Cuts at: 5945 7899 5945
Size: 1954 8929
AclI AA'CG_TT
Cuts at: 10723 10723
Size: 10883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1286 1615 4572 4634 5423 5509 6733 8708
Size: 1012 329 2957 62 789 86 1224 1975
Cuts at: 8708 9140 9887 10155 274
Size: 432 747 268 1002
Fragments arranged by size:
2957 1975 1224 1012 1002 789 747 432
329 268 86 62
AflII C'TTAA_G
Cuts at: 2307 4282 2307
Size: 1975 8908
AflIII A'CryG_T
Cuts at: 8235 9339 10316 8235
Size: 1104 977 8802
Fragments arranged by size:
8802 1104 977
AgeI A'CCGG_T
Cuts at: 9271 9271
Size: 10883
AleI CACnn'nnGTG
Cuts at: 3562 4916 5803 3562
Size: 1354 887 8642
Fragments arranged by size:
8642 1354 887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 291 1678 1781 291
Size: 1387 103 9393
Fragments arranged by size:
9393 1387 103
AlwNI CAG_nnn'CTG
Cuts at: 292 585 2374 3321 3693 4543 7225 7540 8188
Size: 293 1789 947 372 850 2682 315 648
Cuts at: 8188 9755 292
Size: 1567 1420
Fragments arranged by size:
2682 1789 1567 1420 947 850 648 372
315 293
ApaLI G'TGCA_C
Cuts at: 9653 9653
Size: 10883
ApoI r'AATT_y
Cuts at: 435 1005 1042 1458 1814 1885 3192 3376 3384
Size: 570 37 416 356 71 1307 184 8
Cuts at: 3384 3957 5183 5753 6023 7887 10236 10492 435
Size: 573 1226 570 270 1864 2349 256 826
Fragments arranged by size:
2349 1864 1307 1226 826 573 570 570
416 356 270 256 184 71 37 8
AseI AT'TA_AT
Cuts at: 20 7376 20
Size: 7356 3527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10883
AvaI C'yCGr_G
Cuts at: 168 1181 7903 168
Size: 1013 6722 3148
Fragments arranged by size:
6722 3148 1013
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4847 4880 4847
Size: 33 10850
BamHI G'GATC_C
Cuts at: 7908 7908
Size: 10883
BanI G'GyrC_C
Cuts at: 3183 5945 7843 7899 8313 8561 8596 3183
Size: 2762 1898 56 414 248 35 5470
Fragments arranged by size:
5470 2762 1898 414 248 56 35
BanII G_rGCy'C
Cuts at: 229 1052 7897 8927 229
Size: 823 6845 1030 2185
Fragments arranged by size:
6845 2185 1030 823
BbeI G_GCGC'C
Cuts at: 7847 8317 8565 7847
Size: 470 248 10165
Fragments arranged by size:
10165 470 248
BbsI GAAGACnn'nnnn_
Cuts at: 475 3350 4525 4703 6636 475
Size: 2875 1175 178 1933 4722
Fragments arranged by size:
4722 2875 1933 1175 178
BbvCI CC'TCA_GC
Cuts at: 1211 6509 1211
Size: 5298 5585
BciVI GTATCCnnnnn_n'
Cuts at: 2514 4074 5064 8781 9542 10883 2514
Size: 1560 990 3717 761 1341 2514
Fragments arranged by size:
3717 2514 1560 1341 990 761
BclI T'GATC_A
Cuts at: 698 4920 8403 698
Size: 4222 3483 3178
Fragments arranged by size:
4222 3483 3178
BfrBI ATG'CAT
Cuts at: 1758 1758
Size: 10883
BglI GCCn_nnn'nGGC
Cuts at: 2638 2638
Size: 10883
BglII A'GATC_T
Cuts at: 855 8398 10435 855
Size: 7543 2037 1303
Fragments arranged by size:
7543 2037 1303
BlpI GC'TnA_GC
Cuts at: 223 7499 7995 223
Size: 7276 496 3111
Fragments arranged by size:
7276 3111 496
Bme1580I G_kGCm'C
Cuts at: 2634 2814 8508 8601 9657 2634
Size: 180 5694 93 1056 3860
Fragments arranged by size:
5694 3860 1056 180 93
BmgBI CAC'GTC
Cuts at: 1471 1471
Size: 10883
BmrI ACTGGGnnnn_n'
Cuts at: 195 336 3896 4979 8095 8176 8511 10655 195
Size: 141 3560 1083 3116 81 335 2144 423
Fragments arranged by size:
3560 3116 2144 1083 423 335 141 81
BmtI G_CTAG'C
Cuts at: 5741 5741
Size: 10883
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9250 9250
Size: 10883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1735 1964 3308 3944 4325 5540 7190 562
Size: 1173 229 1344 636 381 1215 1650 4255
Fragments arranged by size:
4255 1650 1344 1215 1173 636 381 229
Bpu10I CC'TnA_GC
Cuts at: 1211 6509 6619 1211
Size: 5298 110 5475
Fragments arranged by size:
5475 5298 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5547 6902 8059 8938 9430 9728 5547
Size: 1355 1157 879 492 298 6702
Fragments arranged by size:
6702 1355 1157 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1417 5311 6172 6442 7579 10587 1417
Size: 3894 861 270 1137 3008 1713
Fragments arranged by size:
3894 3008 1713 1137 861 270
BsaAI yAC'GTr
Cuts at: 8236 8236
Size: 10883
BsaBI GATnn'nnATC
Cuts at: 6 2105 4598 8397 10434 6
Size: 2099 2493 3799 2037 455
Fragments arranged by size:
3799 2493 2099 2037 455
BsaHI Gr'CG_yC
Cuts at: 7844 8314 8562 9264 10760 7844
Size: 470 248 702 1496 7967
Fragments arranged by size:
7967 1496 702 470 248
BsaWI w'CCGG_w
Cuts at: 8072 8593 9271 9545 9692 10103 8072
Size: 521 678 274 147 411 8852
Fragments arranged by size:
8852 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1670 1700 3578 3608 5626 5656 7163 7193 1670
Size: 30 1878 30 2018 30 1507 30 5360
Fragments arranged by size:
5360 2018 1878 1507 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1390 3599 4946 7021 7712 1390
Size: 2209 1347 2075 691 4561
Fragments arranged by size:
4561 2209 2075 1347 691
BseYI C'CCAG_C
Cuts at: 463 768 5825 6624 7850 8308 9066 9643 10139
Size: 305 5057 799 1226 458 758 577 496
Cuts at: 10139 463
Size: 1207
Fragments arranged by size:
5057 1226 1207 799 758 577 496 458
305
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 657 1451 1758 5197 657
Size: 794 307 3439 6343
Fragments arranged by size:
6343 3439 794 307
BsiEI CG_ry'CG
Cuts at: 104 8425 8471 9679 104
Size: 8321 46 1208 1308
Fragments arranged by size:
8321 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4910 7897 8675 8865 9657 4910
Size: 2987 778 190 792 6136
Fragments arranged by size:
6136 2987 792 778 190
BsmI GAATG_Cn'
Cuts at: 280 948 1240 2958 5874 6225 7034 280
Size: 668 292 1718 2916 351 809 4129
Fragments arranged by size:
4129 2916 1718 809 668 351 292
BsmBI CGTCTCn'nnnn_
Cuts at: 1096 7153 7409 1096
Size: 6057 256 4570
Fragments arranged by size:
6057 4570 256
Bsp1286I G_dGCh'C
Cuts at: 229 1052 2634 2814 4910 7897 8508 8601 8675
Size: 823 1582 180 2096 2987 611 93 74
Cuts at: 8675 8865 8927 9657 229
Size: 190 62 730 1455
Fragments arranged by size:
2987 2096 1582 1455 823 730 611 190
180 93 74 62
BspEI T'CCGG_A
Cuts at: 10103 10103
Size: 10883
BspHI T'CATG_A
Cuts at: 3708 5247 6150 7385 10059 3708
Size: 1539 903 1235 2674 4532
Fragments arranged by size:
4532 2674 1539 1235 903
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1473 2018 3701 7933 8147 8449 8830 1351
Size: 122 545 1683 4232 214 302 381 3404
Fragments arranged by size:
4232 3404 1683 545 381 302 214 122
BsrBI CCG'CTC
Cuts at: 50 9175 9229 10713 10878 50
Size: 9125 54 1484 165 55
Fragments arranged by size:
9125 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1175 2027 2803 7190 8795 1175
Size: 852 776 4387 1605 3263
Fragments arranged by size:
4387 3263 1605 852 776
BsrFI r'CCGG_y
Cuts at: 8881 9062 9271 8881
Size: 181 209 10493
Fragments arranged by size:
10493 209 181
BssSI C'ACGA_G
Cuts at: 9154 9512 9154
Size: 358 10525
BstAPI GCAn_nnn'nTGC
Cuts at: 2569 8260 2569
Size: 5691 5192
BstBI TT'CG_AA
Cuts at: 1485 9244 1485
Size: 7759 3124
BstXI CCAn_nnnn'nTGG
Cuts at: 367 2412 3564 5667 367
Size: 2045 1152 2103 5583
Fragments arranged by size:
5583 2103 2045 1152
BstYI r'GATC_y
Cuts at: 1 729 855 3526 5913 6069 6096 7118 7908
Size: 728 126 2671 2387 156 27 1022 790
Cuts at: 7908 8375 8398 8733 8979 9991 10077 10089 10435
Size: 467 23 335 246 1012 86 12 346
Cuts at: 10435 1
Size: 449
Fragments arranged by size:
2671 2387 1022 1012 790 728 467 449
346 335 246 156 126 86 27 23
12
Bsu36I CC'TnA_GG
Cuts at: 5747 6074 5747
Size: 327 10556
BtgI C'CryG_G
Cuts at: 1336 6944 7611 1336
Size: 5608 667 4608
Fragments arranged by size:
5608 4608 667
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6489 8833 9014 9177 6489
Size: 2344 181 163 8195
Fragments arranged by size:
8195 2344 181 163
BtsI GCAGTG_nn'
Cuts at: 195 2648 4317 5854 6225 6815 8126 195
Size: 2453 1669 1537 371 590 1311 2952
Fragments arranged by size:
2952 2453 1669 1537 1311 590 371
ClaI AT'CG_AT
Cuts at: 2109 2109
Size: 10883
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2567 2602 2567
Size: 35 10848
DraI TTT'AAA
Cuts at: 4397 6659 7882 4397
Size: 2262 1223 7398
Fragments arranged by size:
7398 2262 1223
DraIII CAC_nnn'GTG
Cuts at: 1474 1474
Size: 10883
DrdI GACnn_nn'nnGTC
Cuts at: 8589 9447 8589
Size: 858 10025
EaeI y'GGCC_r
Cuts at: 1955 2246 6471 8468 8642 9033 9060 10405 1955
Size: 291 4225 1997 174 391 27 1345 2433
Fragments arranged by size:
4225 2433 1997 1345 391 291 174 27
EagI C'GGCC_G
Cuts at: 8468 8468
Size: 10883
EarI CTCTTCn'nnn_
Cuts at: 657 1026 2171 2648 4168 6125 6588 8906 9116
Size: 369 1145 477 1520 1957 463 2318 210
Cuts at: 9116 657
Size: 2424
Fragments arranged by size:
2424 2318 1957 1520 1145 477 463 369
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9091 9401 9547 10693 9091
Size: 310 146 1146 9281
Fragments arranged by size:
9281 1146 310 146
EcoICRI GAG'CTC
Cuts at: 7895 7895
Size: 10883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 562 1286 1615 1735 1964 3308 3944 4325
Size: 288 724 329 120 229 1344 636 381
Cuts at: 4325 4572 4634 5423 5509 5540 6733 7190 8708
Size: 247 62 789 86 31 1193 457 1518
Cuts at: 8708 9140 9887 10155 274
Size: 432 747 268 1002
Fragments arranged by size:
1518 1344 1193 1002 789 747 724 636
457 432 381 329 288 268 247 229
120 86 62 31
EcoNI CCTnn'n_nnAGG
Cuts at: 712 6686 7070 712
Size: 5974 384 4525
Fragments arranged by size:
5974 4525 384
EcoO109I rG'GnC_Cy
Cuts at: 8022 8022
Size: 10883
EcoRI G'AATT_C
Cuts at: 1814 1885 7887 1814
Size: 71 6002 4810
Fragments arranged by size:
6002 4810 71
EcoRV GAT'ATC
Cuts at: 1173 1386 4332 1173
Size: 213 2946 7724
Fragments arranged by size:
7724 2946 213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4660 5942 6929 6955 8902 4660
Size: 1282 987 26 1947 6641
Fragments arranged by size:
6641 1947 1282 987 26
FspI TGC'GCA
Cuts at: 8664 8664
Size: 10883
HaeII r_GCGC'y
Cuts at: 7847 8317 8565 9587 10566 7847
Size: 470 248 1022 979 8164
Fragments arranged by size:
8164 1022 979 470 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 1527 1559 2220 2252 4072 4104 5038
Size: 32 1344 32 661 32 1820 32 934
Cuts at: 5038 5070 5242 5274 5645 5677 7110 7142 7207
Size: 32 172 32 371 32 1433 32 65
Cuts at: 7207 7239 8725 8755 8757 8787 151
Size: 32 1486 30 2 30 2247
Fragments arranged by size:
2247 1820 1486 1433 1344 934 661 371
172 65 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1507 4751 5552 7566 7922 1507
Size: 3244 801 2014 356 4468
Fragments arranged by size:
4468 3244 2014 801 356
HindIII A'AGCT_T
Cuts at: 524 3829 4811 5514 524
Size: 3305 982 703 5893
Fragments arranged by size:
5893 3305 982 703
HpaI GTT'AAC
Cuts at: 5552 7566 5552
Size: 2014 8869
KasI G'GCGC_C
Cuts at: 7843 8313 8561 7843
Size: 470 248 10165
Fragments arranged by size:
10165 470 248
KpnI G_GTAC'C
Cuts at: 5949 7903 5949
Size: 1954 8929
MfeI C'AATT_G
Cuts at: 677 3813 6443 677
Size: 3136 2630 5117
Fragments arranged by size:
5117 3136 2630
MluI A'CGCG_T
Cuts at: 10316 10316
Size: 10883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 103 981 1963 2429 4209 5353 5790 9554 9738
Size: 878 982 466 1780 1144 437 3764 184
Cuts at: 9738 103
Size: 1248
Fragments arranged by size:
3764 1780 1248 1144 982 878 466 437
184
MscI TGG'CCA
Cuts at: 2248 6473 8644 2248
Size: 4225 2171 4487
Fragments arranged by size:
4487 4225 2171
MslI CAynn'nnrTG
Cuts at: 1763 3562 4673 4916 4995 5803 7156 8872 8999
Size: 1799 1111 243 79 808 1353 1716 127
Cuts at: 8999 1763
Size: 3647
Fragments arranged by size:
3647 1799 1716 1353 1111 808 243 127
79
MspA1I CmG'CkG
Cuts at: 265 1658 1984 2371 3922 6753 7540 7850 7994
Size: 1393 326 387 1551 2831 787 310 144
Cuts at: 7994 8308 8668 9681 9926 265
Size: 314 360 1013 245 1222
Fragments arranged by size:
2831 1551 1393 1222 1013 787 387 360
326 314 310 245 144
NaeI GCC'GGC
Cuts at: 9064 9064
Size: 10883
NarI GG'CG_CC
Cuts at: 7844 8314 8562 7844
Size: 470 248 10165
Fragments arranged by size:
10165 470 248
NcoI C'CATG_G
Cuts at: 1336 6944 7611 1336
Size: 5608 667 4608
Fragments arranged by size:
5608 4608 667
NdeI CA'TA_TG
Cuts at: 764 4996 764
Size: 4232 6651
NgoMIV G'CCGG_C
Cuts at: 9062 9062
Size: 10883
NheI G'CTAG_C
Cuts at: 5737 5737
Size: 10883
NsiI A_TGCA'T
Cuts at: 1760 1760
Size: 10883
NspI r_CATG'y
Cuts at: 2834 6086 7050 7057 7340 7936 8436 9343 2834
Size: 3252 964 7 283 596 500 907 4374
Fragments arranged by size:
4374 3252 964 907 596 500 283 7
PciI A'CATG_T
Cuts at: 9339 9339
Size: 10883
PflMI CCAn_nnn'nTGG
Cuts at: 3922 3922
Size: 10883
PfoI T'CCnGG_A
Cuts at: 422 1268 5025 422
Size: 846 3757 6280
Fragments arranged by size:
6280 3757 846
PmeI GTTT'AAAC
Cuts at: 7882 7882
Size: 10883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2516 2548 10022 10054 2516
Size: 32 7474 32 3345
Fragments arranged by size:
7474 3345 32 32
PsiI TTA'TAA
Cuts at: 1193 1529 2900 1193
Size: 336 1371 9176
Fragments arranged by size:
9176 1371 336
PstI C_TGCA'G
Cuts at: 270 2771 5861 6399 7020 7930 8615 270
Size: 2501 3090 538 621 910 685 2538
Fragments arranged by size:
3090 2538 2501 910 685 621 538
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10883
PvuII CAG'CTG
Cuts at: 265 1658 1984 2371 3922 7540 7850 8308 8668
Size: 1393 326 387 1551 3618 310 458 360
Cuts at: 8668 265
Size: 2480
Fragments arranged by size:
3618 2480 1551 1393 458 387 360 326
310
RsrII CG'GwC_CG
Cuts at: 9078 9078
Size: 10883
SacI G_AGCT'C
Cuts at: 7897 7897
Size: 10883
SalI G'TCGA_C
Cuts at: 1505 7920 1505
Size: 6415 4468
SapI GCTCTTCn'nnn_
Cuts at: 4168 8906 9116 4168
Size: 4738 210 5935
Fragments arranged by size:
5935 4738 210
SbfI CC_TGCA'GG
Cuts at: 7930 7930
Size: 10883
ScaI AGT'ACT
Cuts at: 3911 4898 6352 6393 10400 3911
Size: 987 1454 41 4007 4394
Fragments arranged by size:
4394 4007 1454 987 41
SexAI A'CCwGG_T
Cuts at: 2492 5409 2492
Size: 2917 7966
SfcI C'TryA_G
Cuts at: 33 266 284 2767 4289 4859 5857 6395 6821
Size: 233 18 2483 1522 570 998 538 426
Cuts at: 6821 6923 7016 7926 8611 9604 9795 33
Size: 102 93 910 685 993 191 1121
Fragments arranged by size:
2483 1522 1121 998 993 910 685 570
538 426 233 191 102 93 18
SfoI GGC'GCC
Cuts at: 7845 8315 8563 7845
Size: 470 248 10165
Fragments arranged by size:
10165 470 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10883
SmaI CCC'GGG
Cuts at: 7905 7905
Size: 10883
SmlI C'TyrA_G
Cuts at: 1181 2307 4282 5562 6917 8038 8953 9445 9707
Size: 1126 1975 1280 1355 1121 915 492 262
Cuts at: 9707 1181
Size: 2357
Fragments arranged by size:
2357 1975 1355 1280 1126 1121 915 492
262
SpeI A'CTAG_T
Cuts at: 67 3559 6643 6894 67
Size: 3492 3084 251 4056
Fragments arranged by size:
4056 3492 3084 251
SphI G_CATG'C
Cuts at: 6086 7050 7340 7936 8436 6086
Size: 964 290 596 500 8533
Fragments arranged by size:
8533 964 596 500 290
SspI AAT'ATT
Cuts at: 2583 2936 3969 6852 2583
Size: 353 1033 2883 6614
Fragments arranged by size:
6614 2883 1033 353
StuI AGG'CCT
Cuts at: 1858 6457 6935 1858
Size: 4599 478 5806
Fragments arranged by size:
5806 4599 478
StyI C'CwwG_G
Cuts at: 240 1336 4914 5383 5801 6050 6186 6561 6944
Size: 1096 3578 469 418 249 136 375 383
Cuts at: 6944 7527 7611 8017 8371 240
Size: 583 84 406 354 2752
Fragments arranged by size:
3578 2752 1096 583 469 418 406 383
375 354 249 136 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2685 5337 7658 8466 8599 9058 9267 2685
Size: 2652 2321 808 133 459 209 4301
Fragments arranged by size:
4301 2652 2321 808 459 209 133
TatI w'GTAC_w
Cuts at: 1741 1897 2827 3909 4896 6350 6391 10398 1741
Size: 156 930 1082 987 1454 41 4007 2226
Fragments arranged by size:
4007 2226 1454 1082 987 930 156 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2793 2825 3123 3155 5626 5658 10400 10432 2793
Size: 32 298 32 2471 32 4742 32 3244
Fragments arranged by size:
4742 3244 2471 298 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3321 8680 3321
Size: 5359 5524
XbaI T'CTAG_A
Cuts at: 1512 3973 5064 7914 1512
Size: 2461 1091 2850 4481
Fragments arranged by size:
4481 2850 2461 1091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1148 8378 1148
Size: 7230 3653
XhoI C'TCGA_G
Cuts at: 1181 1181
Size: 10883
XmaI C'CCGG_G
Cuts at: 7903 7903
Size: 10883
XmnI GAAnn'nnTTC
Cuts at: 3911 4532 4643 6311 6970 3911
Size: 621 111 1668 659 7824
Fragments arranged by size:
7824 1668 659 621 111
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BcgI BsiWI BsrGI BssHII BstEII BstZ17I FseI FspAI
NotI NruI PacI PasI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI ZraI