Restriction map for
pF1KE2700
(Circular) (Six-base) MAPSORT of: pF1KE2700.seq Check: 4814 from: 1 to: 4673
pF1KE2700 4673 bp
With 182 enzymes: SgfI *
July 6, 2017 13:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1313 1313
Size: 4673
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1398 1689 1398
Size: 291 4382
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 2930 3677 3945 2498
Size: 432 747 268 3226
Fragments arranged by size:
3226 747 432 268
AfeI AGC'GCT
Cuts at: 834 834
Size: 4673
AflIII A'CryG_T
Cuts at: 2025 3129 4106 2025
Size: 1104 977 2592
Fragments arranged by size:
2592 1104 977
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 361 496 1450 1978 3545 361
Size: 135 954 528 1567 1489
Fragments arranged by size:
1567 1489 954 528 135
ApaI G_GGCC'C
Cuts at: 828 828
Size: 4673
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 1350 1693 1350
Size: 343 4330
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 451 469 1186 1398 1689 2103 2351 2386 451
Size: 18 717 212 291 414 248 35 2738
Fragments arranged by size:
2738 717 414 291 248 212 35 18
BanII G_rGCy'C
Cuts at: 828 934 1177 1687 2717 828
Size: 106 243 510 1030 2784
Fragments arranged by size:
2784 1030 510 243 106
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 1203 1338 1203
Size: 135 4538
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 472 574 828 2298 2391 3447 472
Size: 102 254 1470 93 1056 1698
Fragments arranged by size:
1698 1470 1056 254 102 93
BmrI ACTGGGnnnn_n'
Cuts at: 1493 1885 1966 2301 4445 1493
Size: 392 81 335 2144 1721
Fragments arranged by size:
2144 1721 392 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 3040 195
Size: 2845 1828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 362 409 727 776 962 1006 305
Size: 57 47 318 49 186 44 3972
Fragments arranged by size:
3972 318 186 57 49 47 44
Bpu10I CC'TnA_GC
Cuts at: 928 928
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 2728 3220 3518 1849
Size: 879 492 298 3004
Fragments arranged by size:
3004 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 4377 522
Size: 3855 818
BsaAI yAC'GTr
Cuts at: 2026 2026
Size: 4673
BsaBI GATnn'nnATC
Cuts at: 6 2187 4224 6
Size: 2181 2037 455
Fragments arranged by size:
2181 2037 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1526 1862 2383 3061 3335 3482 3893 1526
Size: 336 521 678 274 147 411 2306
Fragments arranged by size:
2306 678 521 411 336 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 811 436
Size: 375 4298
BseYI C'CCAG_C
Cuts at: 1147 2098 2856 3433 3929 1147
Size: 951 758 577 496 1891
Fragments arranged by size:
1891 951 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1687 2465 2655 3447 1687
Size: 778 190 792 2913
Fragments arranged by size:
2913 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 472 574 828 934 1177 1687 2298 2391 2465
Size: 102 254 106 243 510 611 93 74
Cuts at: 2465 2655 2717 3447 472
Size: 190 62 730 1698
Fragments arranged by size:
1698 730 611 510 254 243 190 106
102 93 74 62
BspEI T'CCGG_A
Cuts at: 1526 3893 1526
Size: 2367 2306
BspHI T'CATG_A
Cuts at: 407 3849 407
Size: 3442 1231
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1313 1723 1937 2239 2620 1313
Size: 410 214 302 381 3366
Fragments arranged by size:
3366 410 381 302 214
BsrBI CCG'CTC
Cuts at: 50 153 330 2965 3019 4503 4668 50
Size: 103 177 2635 54 1484 165 55
Fragments arranged by size:
2635 1484 177 165 103 55 54
BsrDI GCAATG_nn'
Cuts at: 230 1347 2585 230
Size: 1117 1238 2318
Fragments arranged by size:
2318 1238 1117
BsrFI r'CCGG_y
Cuts at: 2671 2852 3061 2671
Size: 181 209 4283
Fragments arranged by size:
4283 209 181
BssSI C'ACGA_G
Cuts at: 1153 2944 3302 1153
Size: 1791 358 2524
Fragments arranged by size:
2524 1791 358
BstAPI GCAn_nnn'nTGC
Cuts at: 496 2050 496
Size: 1554 3119
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 868 868
Size: 4673
BstYI r'GATC_y
Cuts at: 1 551 1610 1698 2165 2188 2523 2769 3781
Size: 550 1059 88 467 23 335 246 1012
Cuts at: 3781 3867 3879 4225 1
Size: 86 12 346 449
Fragments arranged by size:
1059 1012 550 467 449 346 335 246
88 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 464 464
Size: 4673
BtgI C'CryG_G
Cuts at: 238 415 590 761 1544 238
Size: 177 175 171 783 3367
Fragments arranged by size:
3367 783 177 175 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 572 1916 572
Size: 1344 3329
DraI TTT'AAA
Cuts at: 1672 1672
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 756 1381 2379 3237 756
Size: 625 998 858 2192
Fragments arranged by size:
2192 998 858 625
EaeI y'GGCC_r
Cuts at: 440 1491 1552 1664 2258 2432 2823 2850 4195
Size: 1051 61 112 594 174 391 27 1345
Cuts at: 4195 440
Size: 918
Fragments arranged by size:
1345 1051 918 594 391 174 112 61
27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 263 1471 2696 2906 263
Size: 1208 1225 210 2030
Fragments arranged by size:
2030 1225 1208 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1685 1685
Size: 4673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 305 362 409 727 776 962 1006 2498 2930
Size: 57 47 318 49 186 44 1492 432
Cuts at: 2930 3677 3945 305
Size: 747 268 1033
Fragments arranged by size:
1492 1033 747 432 318 268 186 57
49 47 44
EcoO109I rG'GnC_Cy
Cuts at: 433 1447 1812 433
Size: 1014 365 3294
Fragments arranged by size:
3294 1014 365
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 836 2107 2355 3377 4356 836
Size: 1271 248 1022 979 1153
Fragments arranged by size:
1271 1153 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2515 2545 2547 2577 2515
Size: 30 2 30 4611
Fragments arranged by size:
4611 30 30 2
HincII GTy'rAC
Cuts at: 1386 1712 1386
Size: 326 4347
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1402 1693 1402
Size: 291 4382
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 376 683 3344 3528 376
Size: 307 2661 184 1521
Fragments arranged by size:
2661 1521 307 184
MscI TGG'CCA
Cuts at: 1493 2434 1493
Size: 941 3732
MslI CAynn'nnrTG
Cuts at: 766 2662 2789 766
Size: 1896 127 2650
Fragments arranged by size:
2650 1896 127
MspA1I CmG'CkG
Cuts at: 1560 1784 2098 2458 3471 3716 1560
Size: 224 314 360 1013 245 2517
Fragments arranged by size:
2517 1013 360 314 245 224
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 238 590 761 1544 238
Size: 352 171 783 3367
Fragments arranged by size:
3367 783 352 171
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NspI r_CATG'y
Cuts at: 1726 2226 3133 1726
Size: 500 907 3266
Fragments arranged by size:
3266 907 500
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PflMI CCAn_nnn'nTGG
Cuts at: 1290 1512 1290
Size: 222 4451
PfoI T'CCnGG_A
Cuts at: 908 1070 1370 908
Size: 162 300 4211
Fragments arranged by size:
4211 300 162
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PpuMI rG'GwC_Cy
Cuts at: 1447 1447
Size: 4673
PshAI GACnn'nnGTC
Cuts at: 1091 1244 1091
Size: 153 4520
PspOMI G'GGCC_C
Cuts at: 824 824
Size: 4673
PstI C_TGCA'G
Cuts at: 492 1112 1720 2405 492
Size: 620 608 685 2760
Fragments arranged by size:
2760 685 620 608
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 1560 2098 2458 1560
Size: 538 360 3775
Fragments arranged by size:
3775 538 360
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 1687 1687
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 2696 2906 2696
Size: 210 4463
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 1195 4190 1195
Size: 2995 1678
SexAI A'CCwGG_T
Cuts at: 1449 1449
Size: 4673
SfcI C'TryA_G
Cuts at: 33 488 1098 1108 1716 2401 3394 3585 33
Size: 455 610 10 608 685 993 191 1121
Fragments arranged by size:
1121 993 685 610 608 455 191 10
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1352 1695 1352
Size: 343 4330
SmlI C'TyrA_G
Cuts at: 1828 2743 3235 3497 1828
Size: 915 492 262 3004
Fragments arranged by size:
3004 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
StyI C'CwwG_G
Cuts at: 238 506 590 761 1511 1544 1807 2161 238
Size: 268 84 171 750 33 263 354 2750
Fragments arranged by size:
2750 750 354 268 263 171 84 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 519 2256 2389 2848 3057 519
Size: 1737 133 459 209 2135
Fragments arranged by size:
2135 1737 459 209 133
TatI w'GTAC_w
Cuts at: 983 1193 1649 4188 983
Size: 210 456 2539 1468
Fragments arranged by size:
2539 1468 456 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 908 940 1352 1384 1556 1588 4190 4222 908
Size: 32 412 32 172 32 2602 32 1359
Fragments arranged by size:
2602 1359 412 172 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 125 598 2470 125
Size: 473 1872 2328
Fragments arranged by size:
2328 1872 473
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 199 2168 199
Size: 1969 2704
XmaI C'CCGG_G
Cuts at: 1350 1693 1350
Size: 343 4330
XmnI GAAnn'nnTTC
Cuts at: 1591 1591
Size: 4673
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI