Restriction map for
pF1KE2698
(Circular) (Six-base) MAPSORT of: pF1KE2698.seq Check: 8226 from: 1 to: 5423
pF1KE2698 5423 bp
With 182 enzymes: SgfI *
June 29, 2017 17:53 ..
AccI GT'mk_AC
Cuts at: 2461 2461
Size: 5423
Acc65I G'GTAC_C
Cuts at: 146 2439 146
Size: 2293 3130
AclI AA'CG_TT
Cuts at: 5263 5263
Size: 5423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 535 916 2213 2245 3248 3680 4427 4695
Size: 174 381 1297 32 1003 432 747 268
Cuts at: 4695 361
Size: 1089
Fragments arranged by size:
1297 1089 1003 747 432 381 268 174
32
AfeI AGC'GCT
Cuts at: 2211 2211
Size: 5423
AflIII A'CryG_T
Cuts at: 2048 2775 3879 4856 2048
Size: 727 1104 977 2615
Fragments arranged by size:
2615 1104 977 727
AgeI A'CCGG_T
Cuts at: 3811 3811
Size: 5423
AleI CACnn'nnGTG
Cuts at: 271 1282 1975 271
Size: 1011 693 3719
Fragments arranged by size:
3719 1011 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 869 1114 1942 869
Size: 245 828 4350
Fragments arranged by size:
4350 828 245
AlwNI CAG_nnn'CTG
Cuts at: 207 469 537 714 2040 2728 4295 207
Size: 262 68 177 1326 688 1567 1335
Fragments arranged by size:
1567 1335 1326 688 262 177 68
ApaLI G'TGCA_C
Cuts at: 4193 4193
Size: 5423
ApoI r'AATT_y
Cuts at: 1168 1255 1327 1750 2427 4776 5032 1168
Size: 87 72 423 677 2349 256 1559
Fragments arranged by size:
2349 1559 677 423 256 87 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 5423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
AvaI C'yCGr_G
Cuts at: 2443 2443
Size: 5423
BamHI G'GATC_C
Cuts at: 1246 2448 1246
Size: 1202 4221
BanI G'GyrC_C
Cuts at: 146 428 1918 2439 2853 3101 3136 146
Size: 282 1490 521 414 248 35 2433
Fragments arranged by size:
2433 1490 521 414 282 248 35
BanII G_rGCy'C
Cuts at: 2437 3467 2437
Size: 1030 4393
BbeI G_GCGC'C
Cuts at: 2857 3105 2857
Size: 248 5175
BbsI GAAGACnn'nnnn_
Cuts at: 2233 2233
Size: 5423
BbvCI CC'TCA_GC
Cuts at: 2346 2346
Size: 5423
BciVI GTATCCnnnnn_n'
Cuts at: 3321 4082 5423 3321
Size: 761 1341 3321
Fragments arranged by size:
3321 1341 761
BclI T'GATC_A
Cuts at: 1421 2943 1421
Size: 1522 3901
BfrBI ATG'CAT
Cuts at: 1101 1101
Size: 5423
BglI GCCn_nnn'nGGC
Cuts at: 472 472
Size: 5423
BglII A'GATC_T
Cuts at: 666 2938 4975 666
Size: 2272 2037 1114
Fragments arranged by size:
2272 2037 1114
BlpI GC'TnA_GC
Cuts at: 2535 2535
Size: 5423
Bme1580I G_kGCm'C
Cuts at: 1273 3048 3141 4197 1273
Size: 1775 93 1056 2499
Fragments arranged by size:
2499 1775 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2635 2716 3051 5195 2635
Size: 81 335 2144 2863
Fragments arranged by size:
2863 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1494 2409 3790 1494
Size: 915 1381 3127
Fragments arranged by size:
3127 1381 915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 559 595 979 1042 1898 172
Size: 387 36 384 63 856 3697
Fragments arranged by size:
3697 856 387 384 63 36
Bpu10I CC'TnA_GC
Cuts at: 1630 2346 1630
Size: 716 4707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 628 1441 1920 2599 3478 3970 4268 412
Size: 216 813 479 679 879 492 298 1567
Fragments arranged by size:
1567 879 813 679 492 479 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 1426 1465 5127 1426
Size: 39 3662 1722
Fragments arranged by size:
3662 1722 39
BsaAI yAC'GTr
Cuts at: 798 2776 798
Size: 1978 3445
BsaBI GATnn'nnATC
Cuts at: 6 2937 4974 6
Size: 2931 2037 455
Fragments arranged by size:
2931 2037 455
BsaHI Gr'CG_yC
Cuts at: 1133 1531 1961 2854 3102 3804 5300 1133
Size: 398 430 893 248 702 1496 1256
Fragments arranged by size:
1496 1256 893 702 430 398 248
BsaWI w'CCGG_w
Cuts at: 560 2612 3133 3811 4085 4232 4643 560
Size: 2052 521 678 274 147 411 1340
Fragments arranged by size:
2052 1340 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1896 1926 1896
Size: 30 5393
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 835 976 1039 1423 1635 2161 835
Size: 141 63 384 212 526 4097
Fragments arranged by size:
4097 526 384 212 141 63
BseYI C'CCAG_C
Cuts at: 476 2848 3606 4183 4679 476
Size: 2372 758 577 496 1220
Fragments arranged by size:
2372 1220 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 820 1399 592
Size: 228 579 4616
Fragments arranged by size:
4616 579 228
BsiEI CG_ry'CG
Cuts at: 104 1015 2965 3011 4219 104
Size: 911 1950 46 1208 1308
Fragments arranged by size:
1950 1308 1208 911 46
BsiHKAI G_wGCw'C
Cuts at: 1906 2437 3215 3405 4197 1906
Size: 531 778 190 792 3132
Fragments arranged by size:
3132 792 778 531 190
BsmI GAATG_Cn'
Cuts at: 2152 2152
Size: 5423
Bsp1286I G_dGCh'C
Cuts at: 1273 1906 2437 3048 3141 3215 3405 3467 4197
Size: 633 531 611 93 74 190 62 730
Cuts at: 4197 1273
Size: 2499
Fragments arranged by size:
2499 730 633 611 531 190 93 74
62
BspEI T'CCGG_A
Cuts at: 560 4643 560
Size: 4083 1340
BspHI T'CATG_A
Cuts at: 4599 4599
Size: 5423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 2266 2285 2473 2687 2989 3370 374
Size: 1892 19 188 214 302 381 2427
Fragments arranged by size:
2427 1892 381 302 214 188 19
BsrBI CCG'CTC
Cuts at: 50 1080 1884 1890 3715 3769 5253 5418 50
Size: 1030 804 6 1825 54 1484 165 55
Fragments arranged by size:
1825 1484 1030 804 165 55 54 6
BsrDI GCAATG_nn'
Cuts at: 3335 3335
Size: 5423
BsrFI r'CCGG_y
Cuts at: 1010 1109 1129 3421 3602 3811 1010
Size: 99 20 2292 181 209 2622
Fragments arranged by size:
2622 2292 209 181 99 20
BsrGI T'GTAC_A
Cuts at: 1002 1002
Size: 5423
BssSI C'ACGA_G
Cuts at: 3694 4052 3694
Size: 358 5065
BstAPI GCAn_nnn'nTGC
Cuts at: 469 534 705 2800 469
Size: 65 171 2095 3092
Fragments arranged by size:
3092 2095 171 65
BstBI TT'CG_AA
Cuts at: 3784 3784
Size: 5423
BstXI CCAn_nnnn'nTGG
Cuts at: 1526 2103 1526
Size: 577 4846
BstYI r'GATC_y
Cuts at: 1 576 666 1246 2006 2448 2915 2938 3273
Size: 575 90 580 760 442 467 23 335
Cuts at: 3273 3519 4531 4617 4629 4975 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 760 580 575 467 449 442 346
335 246 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1345 1345
Size: 5423
BtgI C'CryG_G
Cuts at: 107 512 2015 2414 107
Size: 405 1503 399 3116
Fragments arranged by size:
3116 1503 405 399
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 802 3373 3554 3717 802
Size: 2571 181 163 2508
Fragments arranged by size:
2571 2508 181 163
BtsI GCAGTG_nn'
Cuts at: 467 728 2666 467
Size: 261 1938 3224
Fragments arranged by size:
3224 1938 261
DraI TTT'AAA
Cuts at: 2422 2422
Size: 5423
DrdI GACnn_nn'nnGTC
Cuts at: 3129 3987 3129
Size: 858 4565
EaeI y'GGCC_r
Cuts at: 245 1012 1619 2170 3008 3182 3573 3600 4945
Size: 767 607 551 838 174 391 27 1345
Cuts at: 4945 245
Size: 723
Fragments arranged by size:
1345 838 767 723 607 551 391 174
27
EagI C'GGCC_G
Cuts at: 1012 3008 1012
Size: 1996 3427
EarI CTCTTCn'nnn_
Cuts at: 559 832 3446 3656 559
Size: 273 2614 210 2326
Fragments arranged by size:
2614 2326 273 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3631 3941 4087 5233 3631
Size: 310 146 1146 3821
Fragments arranged by size:
3821 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2435 2435
Size: 5423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 361 535 559 595 916 979 1042 1898
Size: 189 174 24 36 321 63 63 856
Cuts at: 1898 2213 2245 3248 3680 4427 4695 172
Size: 315 32 1003 432 747 268 900
Fragments arranged by size:
1003 900 856 747 432 321 315 268
189 174 63 63 36 32 24
EcoO109I rG'GnC_Cy
Cuts at: 993 1626 1708 2562 993
Size: 633 82 854 3854
Fragments arranged by size:
3854 854 633 82
EcoRI G'AATT_C
Cuts at: 1327 2427 1327
Size: 1100 4323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5423
FspI TGC'GCA
Cuts at: 3204 3204
Size: 5423
HaeII r_GCGC'y
Cuts at: 2213 2857 3105 4127 5106 2213
Size: 644 248 1022 979 2530
Fragments arranged by size:
2530 1022 979 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 372 404 1204 1236 1548 1580 1655
Size: 32 130 32 800 32 312 32 75
Cuts at: 1655 1687 3265 3295 3297 3327 210
Size: 32 1578 30 2 30 2306
Fragments arranged by size:
2306 1578 800 312 130 75 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 943 2390 2462 943
Size: 1447 72 3904
Fragments arranged by size:
3904 1447 72
KasI G'GCGC_C
Cuts at: 2853 3101 2853
Size: 248 5175
KpnI G_GTAC'C
Cuts at: 150 2443 150
Size: 2293 3130
MfeI C'AATT_G
Cuts at: 1380 1598 1380
Size: 218 5205
MluI A'CGCG_T
Cuts at: 4856 4856
Size: 5423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1328 1526 4094 4278 1328
Size: 198 2568 184 2473
Fragments arranged by size:
2568 2473 198 184
MscI TGG'CCA
Cuts at: 3184 3184
Size: 5423
MslI CAynn'nnrTG
Cuts at: 271 1096 1282 1975 3412 3539 271
Size: 825 186 693 1437 127 2155
Fragments arranged by size:
2155 1437 825 693 186 127
MspA1I CmG'CkG
Cuts at: 714 1680 1994 2040 2534 2848 3208 4221 4466
Size: 966 314 46 494 314 360 1013 245
Cuts at: 4466 714
Size: 1671
Fragments arranged by size:
1671 1013 966 494 360 314 314 245
46
NaeI GCC'GGC
Cuts at: 3604 3604
Size: 5423
NarI GG'CG_CC
Cuts at: 2854 3102 2854
Size: 248 5175
NcoI C'CATG_G
Cuts at: 107 2414 107
Size: 2307 3116
NdeI CA'TA_TG
Cuts at: 240 240
Size: 5423
NgoMIV G'CCGG_C
Cuts at: 3602 3602
Size: 5423
NsiI A_TGCA'T
Cuts at: 1103 1103
Size: 5423
NspI r_CATG'y
Cuts at: 265 663 1095 1554 2052 2476 2976 3883 265
Size: 398 432 459 498 424 500 907 1805
Fragments arranged by size:
1805 907 500 498 459 432 424 398
PciI A'CATG_T
Cuts at: 2048 3879 2048
Size: 1831 3592
PflMI CCAn_nnn'nTGG
Cuts at: 1541 2016 1541
Size: 475 4948
PfoI T'CCnGG_A
Cuts at: 611 1249 611
Size: 638 4785
PmeI GTTT'AAAC
Cuts at: 2422 2422
Size: 5423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 710 742 4562 4594 710
Size: 32 3820 32 1539
Fragments arranged by size:
3820 1539 32 32
PpuMI rG'GwC_Cy
Cuts at: 993 1626 1708 993
Size: 633 82 4708
Fragments arranged by size:
4708 633 82
PshAI GACnn'nnGTC
Cuts at: 1132 1132
Size: 5423
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1840 1872 1840
Size: 32 5391
PstI C_TGCA'G
Cuts at: 465 1326 2263 2470 3155 465
Size: 861 937 207 685 2733
Fragments arranged by size:
2733 937 861 685 207
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5423
PvuII CAG'CTG
Cuts at: 714 1680 2040 2848 3208 714
Size: 966 360 808 360 2929
Fragments arranged by size:
2929 966 808 360 360
RsrII CG'GwC_CG
Cuts at: 3618 3618
Size: 5423
SacI G_AGCT'C
Cuts at: 2437 2437
Size: 5423
SalI G'TCGA_C
Cuts at: 2460 2460
Size: 5423
SanDI GG'GwC_CC
Cuts at: 993 993
Size: 5423
SapI GCTCTTCn'nnn_
Cuts at: 3446 3656 3446
Size: 210 5213
SbfI CC_TGCA'GG
Cuts at: 2470 2470
Size: 5423
ScaI AGT'ACT
Cuts at: 4940 4940
Size: 5423
SexAI A'CCwGG_T
Cuts at: 1710 1710
Size: 5423
SfcI C'TryA_G
Cuts at: 33 127 171 454 461 1322 2259 2466 3151
Size: 94 44 283 7 861 937 207 685
Cuts at: 3151 4144 4335 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 937 861 685 283 207 191
94 44 7
SfoI GGC'GCC
Cuts at: 2855 3103 2855
Size: 248 5175
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
SmaI CCC'GGG
Cuts at: 2445 2445
Size: 5423
SmlI C'TyrA_G
Cuts at: 391 643 1456 1899 2578 3493 3985 4247 391
Size: 252 813 443 679 915 492 262 1567
Fragments arranged by size:
1567 915 813 679 492 443 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5423
SphI G_CATG'C
Cuts at: 1095 2476 2976 1095
Size: 1381 500 3542
Fragments arranged by size:
3542 1381 500
StuI AGG'CCT
Cuts at: 1454 1454
Size: 5423
StyI C'CwwG_G
Cuts at: 107 214 1449 2141 2414 2557 2911 107
Size: 107 1235 692 273 143 354 2619
Fragments arranged by size:
2619 1235 692 354 273 143 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1450 1965 3006 3139 3598 3807 1450
Size: 515 1041 133 459 209 3066
Fragments arranged by size:
3066 1041 515 459 209 133
TatI w'GTAC_w
Cuts at: 1002 1829 4938 1002
Size: 827 3109 1487
Fragments arranged by size:
3109 1487 827
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1700 1732 1896 1928 4940 4972 1700
Size: 32 164 32 3012 32 2151
Fragments arranged by size:
3012 2151 164 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3220 3220
Size: 5423
XbaI T'CTAG_A
Cuts at: 579 2454 579
Size: 1875 3548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 510 1604 2918 510
Size: 1094 1314 3015
Fragments arranged by size:
3015 1314 1094
XmaI C'CCGG_G
Cuts at: 2443 2443
Size: 5423
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI NheI NotI NruI PacI PasI PmlI
PsiI PspOMI PspXI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI