Restriction map for
pF1KE2692
(Circular) (Six-base) MAPSORT of: pF1KE2692.seq Check: 8166 from: 1 to: 5291
pF1KE2692 5291 bp
With 182 enzymes: SgfI *
June 23, 2017 13:27 ..
AccI GT'mk_AC
Cuts at: 2329 2329
Size: 5291
Acc65I G'GTAC_C
Cuts at: 463 2307 463
Size: 1844 3447
AclI AA'CG_TT
Cuts at: 5131 5131
Size: 5291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 1764 1957 3116 3548 4295 4563 507
Size: 1257 193 1159 432 747 268 1235
Fragments arranged by size:
1257 1235 1159 747 432 268 193
AflIII A'CryG_T
Cuts at: 2643 3747 4724 2643
Size: 1104 977 3210
Fragments arranged by size:
3210 1104 977
AgeI A'CCGG_T
Cuts at: 3679 3679
Size: 5291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1257 1257
Size: 5291
AlwNI CAG_nnn'CTG
Cuts at: 904 1861 2122 2596 4163 904
Size: 957 261 474 1567 2032
Fragments arranged by size:
2032 1567 957 474 261
ApaLI G'TGCA_C
Cuts at: 4061 4061
Size: 5291
ApoI r'AATT_y
Cuts at: 844 1542 2295 4644 4900 844
Size: 698 753 2349 256 1235
Fragments arranged by size:
2349 1235 753 698 256
AscI GG'CGCG_CC
Cuts at: 127 127
Size: 5291
AseI AT'TA_AT
Cuts at: 20 20
Size: 5291
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5291
AvaI C'yCGr_G
Cuts at: 138 2311 138
Size: 2173 3118
BamHI G'GATC_C
Cuts at: 2316 2316
Size: 5291
BanI G'GyrC_C
Cuts at: 156 463 2251 2307 2721 2969 3004 156
Size: 307 1788 56 414 248 35 2443
Fragments arranged by size:
2443 1788 414 307 248 56 35
BanII G_rGCy'C
Cuts at: 278 437 1742 2305 3335 278
Size: 159 1305 563 1030 2234
Fragments arranged by size:
2234 1305 1030 563 159
BbeI G_GCGC'C
Cuts at: 160 2725 2973 160
Size: 2565 248 2478
Fragments arranged by size:
2565 2478 248
BbsI GAAGACnn'nnnn_
Cuts at: 518 2066 518
Size: 1548 3743
BciVI GTATCCnnnnn_n'
Cuts at: 688 3189 3950 5291 688
Size: 2501 761 1341 688
Fragments arranged by size:
2501 1341 761 688
BclI T'GATC_A
Cuts at: 1923 2811 1923
Size: 888 4403
BglI GCCn_nnn'nGGC
Cuts at: 168 168
Size: 5291
BglII A'GATC_T
Cuts at: 2806 4843 2806
Size: 2037 3254
BlpI GC'TnA_GC
Cuts at: 2403 2403
Size: 5291
Bme1580I G_kGCm'C
Cuts at: 2254 2916 3009 4065 2254
Size: 662 93 1056 3480
Fragments arranged by size:
3480 1056 662 93
BmgBI CAC'GTC
Cuts at: 939 939
Size: 5291
BmrI ACTGGGnnnn_n'
Cuts at: 892 2503 2584 2919 5063 892
Size: 1611 81 335 2144 1120
Fragments arranged by size:
2144 1611 1120 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1758 2079 3658 1758
Size: 321 1579 3391
Fragments arranged by size:
3391 1579 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 1236 2185 2227 1207
Size: 29 949 42 4271
Fragments arranged by size:
4271 949 42 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2121 2467 3346 3838 4136 2121
Size: 346 879 492 298 3276
Fragments arranged by size:
3276 879 492 346 298
BsaI GGTCTCn'nnnn_
Cuts at: 403 2207 2222 4995 403
Size: 1804 15 2773 699
Fragments arranged by size:
2773 1804 699 15
BsaAI yAC'GTr
Cuts at: 2644 2644
Size: 5291
BsaBI GATnn'nnATC
Cuts at: 6 2805 4842 6
Size: 2799 2037 455
Fragments arranged by size:
2799 2037 455
BsaHI Gr'CG_yC
Cuts at: 157 1730 2722 2970 3672 5168 157
Size: 1573 992 248 702 1496 280
Fragments arranged by size:
1573 1496 992 702 280 248
BsaWI w'CCGG_w
Cuts at: 1874 2480 3001 3679 3953 4100 4511 1874
Size: 606 521 678 274 147 411 2654
Fragments arranged by size:
2654 678 606 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 956 986 1442 1472 1483 1513 2137 2167 956
Size: 30 456 30 11 30 624 30 4080
Fragments arranged by size:
4080 624 456 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 222 225 286 313 1700 1750 2124 222
Size: 3 61 27 1387 50 374 3389
Fragments arranged by size:
3389 1387 374 61 50 27 3
BseYI C'CCAG_C
Cuts at: 192 2716 3474 4051 4547 192
Size: 2524 758 577 496 936
Fragments arranged by size:
2524 936 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 486 1153 388
Size: 98 667 4526
Fragments arranged by size:
4526 667 98
BsiEI CG_ry'CG
Cuts at: 104 218 2833 2879 4087 104
Size: 114 2615 46 1208 1308
Fragments arranged by size:
2615 1308 1208 114 46
BsiHKAI G_wGCw'C
Cuts at: 278 1222 2305 3083 3273 4065 278
Size: 944 1083 778 190 792 1504
Fragments arranged by size:
1504 1083 944 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1183 1183
Size: 5291
Bsp1286I G_dGCh'C
Cuts at: 278 437 1222 1742 2254 2305 2916 3009 3083
Size: 159 785 520 512 51 611 93 74
Cuts at: 3083 3273 3335 4065 278
Size: 190 62 730 1504
Fragments arranged by size:
1504 785 730 611 520 512 190 159
93 74 62 51
BspEI T'CCGG_A
Cuts at: 4511 4511
Size: 5291
BspHI T'CATG_A
Cuts at: 815 4467 815
Size: 3652 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2341 2555 2857 3238 2341
Size: 214 302 381 4394
Fragments arranged by size:
4394 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1497 3583 3637 5121 5286 50
Size: 1447 2086 54 1484 165 55
Fragments arranged by size:
2086 1484 1447 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1589 3203 1589
Size: 1614 3677
BsrFI r'CCGG_y
Cuts at: 308 3289 3470 3679 308
Size: 2981 181 209 1920
Fragments arranged by size:
2981 1920 209 181
BssHII G'CGCG_C
Cuts at: 127 127
Size: 5291
BssSI C'ACGA_G
Cuts at: 406 2157 3562 3920 406
Size: 1751 1405 358 1777
Fragments arranged by size:
1777 1751 1405 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 2668 1621
Size: 1047 4244
BstBI TT'CG_AA
Cuts at: 560 3652 560
Size: 3092 2199
BstXI CCAn_nnnn'nTGG
Cuts at: 1470 2163 1470
Size: 693 4598
BstYI r'GATC_y
Cuts at: 1 733 942 2316 2783 2806 3141 3387 4399
Size: 732 209 1374 467 23 335 246 1012
Cuts at: 4399 4485 4497 4843 1
Size: 86 12 346 449
Fragments arranged by size:
1374 1012 732 467 449 346 335 246
209 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 454 1573 454
Size: 1119 4172
BtgI C'CryG_G
Cuts at: 107 166 218 266 1115 107
Size: 59 52 48 849 4283
Fragments arranged by size:
4283 849 59 52 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3241 3422 3585 3241
Size: 181 163 4947
Fragments arranged by size:
4947 181 163
BtsI GCAGTG_nn'
Cuts at: 1170 2534 1170
Size: 1364 3927
DraI TTT'AAA
Cuts at: 2290 2290
Size: 5291
DraIII CAC_nnn'GTG
Cuts at: 1957 1957
Size: 5291
DrdI GACnn_nn'nnGTC
Cuts at: 2997 3855 2997
Size: 858 4433
EaeI y'GGCC_r
Cuts at: 215 419 480 1598 2876 3050 3441 3468 4813
Size: 204 61 1118 1278 174 391 27 1345
Cuts at: 4813 215
Size: 693
Fragments arranged by size:
1345 1278 1118 693 391 204 174 61
27
EagI C'GGCC_G
Cuts at: 215 2876 215
Size: 2661 2630
EarI CTCTTCn'nnn_
Cuts at: 382 703 1742 2179 3314 3524 382
Size: 321 1039 437 1135 210 2149
Fragments arranged by size:
2149 1135 1039 437 321 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 265 310 3499 3809 3955 5101 265
Size: 45 3189 310 146 1146 455
Fragments arranged by size:
3189 1146 455 310 146 45
EcoICRI GAG'CTC
Cuts at: 276 2303 276
Size: 2027 3264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 1207 1236 1764 1957 2185 2227 3116 3548
Size: 700 29 528 193 228 42 889 432
Cuts at: 3548 4295 4563 507
Size: 747 268 1235
Fragments arranged by size:
1235 889 747 700 528 432 268 228
193 42 29
EcoO109I rG'GnC_Cy
Cuts at: 518 2430 518
Size: 1912 3379
EcoRI G'AATT_C
Cuts at: 2295 2295
Size: 5291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 732 1362 3310 732
Size: 630 1948 2713
Fragments arranged by size:
2713 1948 630
FspI TGC'GCA
Cuts at: 3072 3072
Size: 5291
HaeII r_GCGC'y
Cuts at: 160 266 1198 2725 2973 3995 4974 160
Size: 106 932 1527 248 1022 979 477
Fragments arranged by size:
1527 1022 979 932 477 248 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 866 898 956 988 1600 1632 1715 1747 3133
Size: 32 58 32 612 32 83 32 1386
Cuts at: 3133 3163 3165 3195 866
Size: 30 2 30 2962
Fragments arranged by size:
2962 1386 612 83 58 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1794 2330 1794
Size: 536 4755
KasI G'GCGC_C
Cuts at: 156 2721 2969 156
Size: 2565 248 2478
Fragments arranged by size:
2565 2478 248
KpnI G_GTAC'C
Cuts at: 467 2311 467
Size: 1844 3447
MluI A'CGCG_T
Cuts at: 4724 4724
Size: 5291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3962 4146 3962
Size: 184 5107
MscI TGG'CCA
Cuts at: 421 1600 3052 421
Size: 1179 1452 2660
Fragments arranged by size:
2660 1452 1179
MslI CAynn'nnrTG
Cuts at: 1558 3280 3407 1558
Size: 1722 127 3442
Fragments arranged by size:
3442 1722 127
MspA1I CmG'CkG
Cuts at: 168 186 220 500 548 1030 2402 2716 3076
Size: 18 34 280 48 482 1372 314 360
Cuts at: 3076 4089 4334 168
Size: 1013 245 1125
Fragments arranged by size:
1372 1125 1013 482 360 314 280 245
48 34 18
NaeI GCC'GGC
Cuts at: 3472 3472
Size: 5291
NarI GG'CG_CC
Cuts at: 157 2722 2970 157
Size: 2565 248 2478
Fragments arranged by size:
2565 2478 248
NcoI C'CATG_G
Cuts at: 107 266 1115 107
Size: 159 849 4283
Fragments arranged by size:
4283 849 159
NgoMIV G'CCGG_C
Cuts at: 3470 3470
Size: 5291
NotI GC'GGCC_GC
Cuts at: 215 215
Size: 5291
NspI r_CATG'y
Cuts at: 934 2344 2844 3751 934
Size: 1410 500 907 2474
Fragments arranged by size:
2474 1410 907 500
PciI A'CATG_T
Cuts at: 3747 3747
Size: 5291
PfoI T'CCnGG_A
Cuts at: 1213 1213
Size: 5291
PmeI GTTT'AAAC
Cuts at: 2290 2290
Size: 5291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4430 4462 4430
Size: 32 5259
PstI C_TGCA'G
Cuts at: 2338 3023 2338
Size: 685 4606
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5291
PvuII CAG'CTG
Cuts at: 500 2716 3076 500
Size: 2216 360 2715
Fragments arranged by size:
2715 2216 360
RsrII CG'GwC_CG
Cuts at: 3486 3486
Size: 5291
SacI G_AGCT'C
Cuts at: 278 2305 278
Size: 2027 3264
SacII CC_GC'GG
Cuts at: 169 221 169
Size: 52 5239
SalI G'TCGA_C
Cuts at: 2328 2328
Size: 5291
SapI GCTCTTCn'nnn_
Cuts at: 382 2179 3314 3524 382
Size: 1797 1135 210 2149
Fragments arranged by size:
2149 1797 1135 210
SbfI CC_TGCA'GG
Cuts at: 2338 2338
Size: 5291
ScaI AGT'ACT
Cuts at: 1777 4808 1777
Size: 3031 2260
SfcI C'TryA_G
Cuts at: 33 2334 3019 4012 4203 33
Size: 2301 685 993 191 1121
Fragments arranged by size:
2301 1121 993 685 191
SfoI GGC'GCC
Cuts at: 158 2723 2971 158
Size: 2565 248 2478
Fragments arranged by size:
2565 2478 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5291
SmaI CCC'GGG
Cuts at: 2313 2313
Size: 5291
SmlI C'TyrA_G
Cuts at: 2136 2446 3361 3853 4115 2136
Size: 310 915 492 262 3312
Fragments arranged by size:
3312 915 492 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5291
SphI G_CATG'C
Cuts at: 934 2344 2844 934
Size: 1410 500 3381
Fragments arranged by size:
3381 1410 500
StuI AGG'CCT
Cuts at: 1571 1571
Size: 5291
StyI C'CwwG_G
Cuts at: 107 266 1115 2425 2779 107
Size: 159 849 1310 354 2619
Fragments arranged by size:
2619 1310 849 354 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1713 1830 2095 2874 3007 3466 3675 1713
Size: 117 265 779 133 459 209 3329
Fragments arranged by size:
3329 779 459 265 209 133 117
TatI w'GTAC_w
Cuts at: 996 1775 4806 996
Size: 779 3031 1481
Fragments arranged by size:
3031 1481 779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2137 2169 4808 4840 2137
Size: 32 2639 32 2588
Fragments arranged by size:
2639 2588 32 32
Tth111I GACn'n_nGTC
Cuts at: 3088 3088
Size: 5291
XbaI T'CTAG_A
Cuts at: 2322 2322
Size: 5291
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2786 2786
Size: 5291
XmaI C'CCGG_G
Cuts at: 2311 2311
Size: 5291
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BsmI BsrGI BstEII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI